Developing a measure of blind patients\u27 interactions with their healthcare providers

Background: People who are blind (PWB) are often perceived as being incompetent with common tasks and functions. When patients detect that their healthcare providers (HCPs) have negative perceptions of them, they often report less satisfaction with their healthcare and disengage in their own healthcare. Objective: A scale assessing the experiences of PWB interacting with HCPs was developed and validated across two studies. Methods: In Study 1, 144 participants completed the scale and provided feedback. In Study 2, 214 participants completed the scale and 4 additional scales to assess construct validity. Results: An exploratory factor analysis in Study 1 revealed a two-factor model consisting of General Quality of Health Care (30.5% variance explained) and Stereotype Content-related items (9.4% variance explained). Study 2 confirmed and validated this two-factor structure (RMSEA (90% CI) = 0.068 (0.057, 0.079), CFI = 0.898, SRMR = 0.066, AIC = 14568.902). Conclusions: This scale is one of the first tools developed from the perspectives of PWB. Results from these studies highlight and elaborate on how PWB perceive that they are viewed by their HCPs in terms of competence and how they perceive to be treated by these HCPs. This scale can be used in training HCPs to better serve their patients with disabilities

Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment

Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death

Important Considerations in Conducting Statistical Mediation Analyses

An investigator wishes to examine mediation in a randomized control trial of the effectiveness of an intervention, which consists of a computerized decision aid for promoting colorectal cancer screening. Mediation is a naturally occurring process, and in any given instance, research investigators seek to ascertain whether it has occurred. In the case of a prevention intervention for a specific chain of events, mediation occurs (1) when the prevention intervention effects a change on a targeted intermediate condition: a mediator, for example, a person’s intentions to get a colorectal screening examination; and (2) when, at a later point in time, this condition effects a change on a targeted outcome, for example, the actual behavior of getting a colorectal screening exam. Full mediation is said to occur when the effectiveness of the intervention on the targeted outcome only takes place through the intermediate condition and does not directly affect the targeted outcome. Partial mediation is said to occur when the intervention causes changes in both the intermediate condition and the targeted outcome. The assessment of mediation is important because conclusions about the efficacy of a public health intervention may depend on how these indirect influences are assessed

The Spanish Version of the Mindful Attention Awareness Scale (MAAS): Measurement Invariance and Psychometric Properties

We examined the measurement invariance and psychometric properties of a Spanish-language version of the Mindful Attention Awareness Scale (MAAS) in a U.S. sample. A total of 609 undergraduate students, including 325 bilingual (Spanish-speaking) participants, from a Hispanic-majority institution completed the assessment battery and were included in the analyses. The MAAS and the Spanish-language Mindful Attention Awareness Scale (MAAS-SP) were shown to be equivalent via multigroup confirmatory factor analysis models testing configural, metric, and scalar invariance. A test of latent mean differences indicated a small, but statistically significant cross-language difference in trait mindfulness as construed by the MAAS, as the latent mean of the MAAS-SP was slightly higher compared with the latent mean of the MAAS. The MAAS-SP evidenced good psychometric properties and concurrent validity. Taken together, findings support the reliability and validity of the MAAS and MAAS-SP in measuring mindfulness across languages. Future studies could continue efforts aimed at examining the structure of mindfulness and its facets across languages