Primer reporte de bacterias termo alcalófilas del manantial “Los Baños del Carmen”, Venustiano Carranza, Chiapas, México

Objective: To isolate and to characterize extremophile microoorganism from hot spring manantial “Baños del Carmen” Venustiano Carranza, Chiapas, México. Design/methodology/approach: The samples were collected in three points of the main pit of the site called "Los Baños del Carmen". Serial dilutions were made and plated in LB medium and nutrient agar. Reseed colonies were isolated with different colonial morphology to obtain axenic cultures. Micro and macroscopic morphological characterization of the isolates and a biochemical characterization with the API 20E kit were carried out. Results: Three alkaline thermophilic bacterial strains were successfully found: YEFP-C, YEPF-J, and YEPF-P. All strains were gram-negative an ability to grow at pH 8-10 and 50?C. Also, all them have a fermentative metabolism of carbohydrates and beta-galactosidase activity. Limitations on study/implications: The strategy used was limited to heterotrophic aerobic organisms; however, it is likely that there is a diversity of other types of prokaryotes and eukaryotes on the site. Findings/conclusions: There are extremophile organisms in hot spring “Los Baños del Carmen”, and this work is the first report with scientific evidence.Objetivo: Aislar y caracterizar microbiológicamente bacterias extremófilas del manantial “Baños del Carmen” Venustiano Carranza, Chiapas, México. Diseño/metodología/aproximación: Las muestras fueron recolectadas en tres puntos de la fosa principal del sitio. Se realizaron diluciones seriadas y se sembraron en medio LB y agar nutritivo. Se llevaron a cabo resiembras de colonias aisladas con diferente morfología colonial hasta obtener cultivos axénicos. Se llevó a cabo una caracterización morfológica micro y macroscópica de los aislados y una caracterización bioquímica con el kit API 20E Resultados: Tres cepas bacterianas termo alcalófilas fueron encontradas: YEFP-C, YEPF-J, y YEPF-P. Todas las cepas fueron Gram negativas y registraron crecimiento en medios con pH de 8.0 a 10.0 y 50 °C. Además, todas tienen metabolismo fermentativo de carbohidratos y actividad beta galactosidase.  Limitaciones del estudio/implicaciones: La estrategia utilizada se limitó a organismos aerobios heterotróficos; sin embargo, es probable que exista biodiversidad de otro tipo de organismos procariotes y eucariotes en el sitio. Hallazgos/conclusiones: Existe vida bacteriana extremofila en el manantial “Los Baños del Carmen”. Este es el primer reporte con evidencia científica

In Silico Analyses of Immune System Protein Interactome Network, Single-Cell RNA Sequencing of Human Tissues, and Artificial Neural Networks Reveal Potential Therapeutic Targets for Drug Repurposing Against COVID-19

There is pressing urgency to better understand the immunological underpinnings of the coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) in order to identify potential therapeutic targets and drugs that allow treating patients effectively. To fill in this gap, we performed in silico analyses of immune system protein interactome network, single-cell RNA sequencing of human tissues, and artificial neural networks to reveal potential therapeutic targets for drug repurposing against COVID-19. As results, the high-confidence protein interactome network was conformed by 1,588 nodes between immune system proteins and human proteins physically associated with SARS-CoV-2. Subsequently, we screened all these nodes in ACE2 and TMPRSS2 co-expressing cells according to the Alexandria Project, finding 75 potential therapeutic targets significantly overexpressed (Z score > 2) in nasal goblet secretory cells, lung type II pneumocytes, and ileal absorptive enterocytes of patients with several immunopathologies. Then, we performed fully connected deep neural networks to find the best multitask classification model to predict the activity of 10,672 drugs for 25 of the 75 aforementioned proteins. On one hand, we obtained 45 approved drugs, 16 compounds under investigation, and 35 experimental compounds with the highest area under the receiver operating characteristic (AUROCs) for 15 immune system proteins. On the other hand, we obtained 4 approved drugs, 9 compounds under investigation, and 16 experimental compounds with the highest multi-target affinities for 9 immune system proteins. In conclusion, computational structure-based drug discovery focused on immune system proteins is imperative to select potential drugs that, after being effectively analyzed in cell lines and clinical trials, these can be considered for treatment of complex symptoms of COVID-19 patients, and for co-therapies with drugs directly targeting SARS-CoV-2

COVID-19 Infection in Pregnancy: PCR Cycle Thresholds, Placental Pathology, and Perinatal Outcomes

(1) This study aimed to evaluate characteristics, perinatal outcomes, and placental pathology of pregnant women with or without SARS-CoV-2 infection in the context of maternal PCR cycle threshold (CT) values. (2) This was a retrospective case-control study in a third-level health center in Mexico City with universal screening by RT-qPCR. The association of COVID-19 manifestations, preeclampsia, and preterm birth with maternal variables and CT values were assessed by logistic regression models and decision trees. (3) Accordingly, 828 and 298 women had a negative and positive test, respectively. Of those positive, only 2.6% of them presented mild to moderate symptoms. Clinical characteristics between both groups of women were similar. No associations between CT values were found for maternal features, such as pre-gestational BMI, age, and symptomatology. A significantly higher percentage of placental fibrinoid was seen with women with low CTs (<25; p < 0.01). Regarding perinatal outcomes, preeclampsia was found to be significantly associated with symptomatology but not with risk factors or CT values (p < 0.01, aOR = 14.72). Moreover, 88.9% of women diagnosed with COVID-19 at <35 gestational weeks and symptomatic developed preeclampsia. (4) The data support strong guidance for pregnancies with SARS-CoV-2 infection, in particular preeclampsia and placental pathology, which need further investigation

La investigación en atención a la diversidad : propuestas de trabajo

Obra colectiva entorno a la educación especial, con una variedad de temas entre los que destacan: la investigación, educación compensatoria, dificultades, ocio, protección, mejoras, el rendimiento escolar, mujeres, diversificación curricular etc. La obra esta dirigida a los profesores, profesionales de la materia y familia.AndalucíaBiblioteca de Educación del Ministerio de Educación, Cultura y Deporte; Calle San Agustín 5 -3 Planta; 28014 Madrid; Tel. +34917748000; [email protected]

Diminishing benefits of urban living for children and adolescents’ growth and development

Optimal growth and development in childhood and adolescence is crucial for lifelong health and well-being1–6. Here we used data from 2,325 population-based studies, with measurements of height and weight from 71 million participants, to report the height and body-mass index (BMI) of children and adolescents aged 5–19 years on the basis of rural and urban place of residence in 200 countries and territories from 1990 to 2020. In 1990, children and adolescents residing in cities were taller than their rural counterparts in all but a few high-income countries. By 2020, the urban height advantage became smaller in most countries, and in many high-income western countries it reversed into a small urban-based disadvantage. The exception was for boys in most countries in sub-Saharan Africa and in some countries in Oceania, south Asia and the region of central Asia, Middle East and north Africa. In these countries, successive cohorts of boys from rural places either did not gain height or possibly became shorter, and hence fell further behind their urban peers. The difference between the age-standardized mean BMI of children in urban and rural areas was <1.1 kg m–2 in the vast majority of countries. Within this small range, BMI increased slightly more in cities than in rural areas, except in south Asia, sub-Saharan Africa and some countries in central and eastern Europe. Our results show that in much of the world, the growth and developmental advantages of living in cities have diminished in the twenty-first century, whereas in much of sub-Saharan Africa they have amplified