Social Bioclimatic Urban House Prototype in Arid Area Energy and Economic Assessment

The energetic and economic evaluation of an evolutionary bioclimatic social house prototype designed in the research unit, for urban arid zones, is presented, calculating their respective costs. The performance of passive systems is evaluated with the Optimix Program, using the Collector Load Rate Method (Los Álamos National Laboratory). The investment alternatives are adopted from the calculation from the Net Present and Marginal Value. The results quantify the use of passive solar systems to make more efficient the thermal and economic benefits of the executed prototypes. The winter thermal load, to which the homes are exposed, is reduced by 64.1%, with technics of energy conservation and the improvement of solar systems and variants applied. Energy savings result in 20% and 22% and the amortization of 2.4 years and 2.3 years, depending on the orientation of the prototype (azimuth 0° N and ± 15° N respectively.Fil: Kurban, Alejandra Silvia. Universidad Nacional de San Juan. Facultad de Arquitectura, Urbanismo y Diseño. Instituto de Estudios en Arquitectura Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Cunsulo, Mario Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina. Universidad Nacional de San Juan. Facultad de Arquitectura, Urbanismo y Diseño. Instituto de Estudios en Arquitectura Ambiental; ArgentinaFil: Matar, M.. Universidad Nacional de San Juan. Facultad de Arquitectura, Urbanismo y Diseño. Instituto de Estudios en Arquitectura Ambiental; ArgentinaFil: Ripoll, V.. Universidad Nacional de San Juan. Facultad de Arquitectura, Urbanismo y Diseño. Instituto de Estudios en Arquitectura Ambiental; ArgentinaFil: Ortega, A.. Universidad Nacional de San Juan. Facultad de Arquitectura, Urbanismo y Diseño. Instituto de Estudios en Arquitectura Ambiental; Argentin

Comparative study of real-time pcr (Taqman probe and sybr green), serological techniques (elisa, ifa and dat) and clinical signs evaluation, for the diagnosis of canine leishmaniasis in experimentally infected dogs

Canine leishmaniasis (CanL) diagnosis is not fully resolved. Currently, two specific methodologies are in continuous development, the detection of the parasite DNA or RNA in target organs and the detection of specific antibodies against Leishmania sp. For a correct diagnosis, it has been shown that the joint use of this type of test is necessary. In this work, a Sybr Green and a TaqMan Probe based on real time PCRs (qPCR) was performed for the detection of Leishmania sp. in order to correlate the results with clinicopathological and serological evaluations (IFA, ELISA and DAT) to propose an optimal biological sample to be used to detect the parasite in both early and late stages of the infection. A total of four samples were processed: conjunctival swabs, popliteal lymph node aspirates, bone marrow aspirates, and peripheral blood from experimentally infected dogs belonging to a larger study. Our results indicated that a single non-invasive sample (conjunctival swab) and the application of both types of qPCR would be reliable for determining Leishmania infection as well as the disease stage in dogs, thus avoiding bone marrow, lymph node aspirate or blood samples collection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)

Software público, libre, abierto, gratuito, privativo y comercial para la innovación y mejora de la calidad en la docencia, investigación, gestión y asistencia

The public free software (now renamed as open software) is the basis of the open source software (OSS) project. This initiative promotes the use of open standards and platform-independent accessibility. Many OSS products are free or low cost, which allows their deployment even in the absence of large economic resources (developing countries, schools, etc.). Besides, their source code can be modified, adapted and optimized by anyone, since it is public. Nevertheless, the OSS may also have drawbacks. Thus, its development may not be as orderly and hierarchical as the privative software is, and may not have an appropriate funding. This may jeopardize the ease of use and coherence of the final product, increasing the transition, training and technical support costs. Besides, it must be taken into account that there is also commercial open software, as well as free privative software. In any case, the excellence of the software may be independent of such variables. Thus, the best strategy is the coexistence and free competition of all kinds of software. This will allow a quality improvement and an innovation enhancement, which will benefit the education, research, management and assistance at the university in particular and at the whole society in general.El software público libre es el fundamento del movimiento de software de fuente abierta (OSS). Éste puede facilitar el uso de estándares abiertos, con accesibilidad independiente de plataforma informática. El bajo coste o gratuidad de muchos productos OSS permite su implantación cuando los recursos económicos son escasos (países en desarrollo, escuelas, etc.). Además, su código se puede modificar, adaptar y mejorar por cualquiera, ya que es público. No obstante, el OSS también puede presentar inconvenientes. Así, su desarrollo puede no ser tan ordenado y jerárquico como el del software privativo y puede no tener una financiación apropiada. De este modo, puede verse comprometida la facilidad de uso y coherencia del producto final. Ello puede incrementar los costes de transición, entrenamiento y soporte técnico. Por otra parte, conviene recordar que existe software libre comercial y software privativo gratuito. En cualquier caso, la excelencia del software puede ser independiente de dichas variables. Por tanto, la mejor estrategia es la coexistencia y libre competencia de todo tipo de software. Así se puede conseguir una mejora de calidad y una potenciación de la innovación, que beneficie a la docencia, investigación, gestión y asistencia universitarias en particular, así como a la sociedad en general

Extinction of Hepatitis C Virus by Ribavirin in Hepatoma Cells Involves Lethal Mutagenesis

Lethal mutagenesis, or virus extinction produced by enhanced mutation rates, is under investigation as an antiviral strategy that aims at counteracting the adaptive capacity of viral quasispecies, and avoiding selection of antiviral-escape mutants. To explore lethal mutagenesis of hepatitis C virus (HCV), it is important to establish whether ribavirin, the purine nucleoside analogue used in anti-HCV therapy, acts as a mutagenic agent during virus replication in cell culture. Here we report the effect of ribavirin during serial passages of HCV in human hepatoma Huh-7.5 cells, regarding viral progeny production and complexity of mutant spectra. Ribavirin produced an increase of mutant spectrum complexity and of the transition types associated with ribavirin mutagenesis, resulting in HCV extinction. Ribavirin-mediated depletion of intracellular GTP was not the major contributory factor to mutagenesis since mycophenolic acid evoked a similar decrease in GTP without an increase in mutant spectrum complexity. The intracellular concentration of the other nucleoside-triphosphates was elevated as a result of ribavirin treatment. Mycophenolic acid extinguished HCV without an intervening mutagenic activity. Ribavirin-mediated, but not mycophenolic acid-mediated, extinction of HCV occurred via a decrease of specific infectivity, a feature typical of lethal mutagenesis. We discuss some possibilities to explain disparate results on ribavirin mutagenesis of HCV

Monitoring dynamics of human adenovirus disassembly induced by mechanical fatigue

The standard pathway for virus infection of eukaryotic cells requires disassembly of the viral shell to facilitate release of the viral genome into the host cell. Here we use mechanical fatigue, well below rupture strength, to induce stepwise disruption of individual human adenovirus particles under physiological conditions, and simultaneously monitor disassembly in real time. Our data show the sequence of dismantling events in individual mature (infectious) and immature (noninfectious) virions, starting with consecutive release of vertex structures followed by capsid cracking and core exposure. Further, our experiments demonstrate that vertex resilience depends inextricably on maturation, and establish the relevance of penton vacancies as seeding loci for virus shell disruption. The mechanical fatigue disruption route recapitulates the adenovirus disassembly pathway in vivo, as well as the stability differences between mature and immature virionsWe acknowledge funding by grants from the Ministry of Science and Innovation of Spain, PIB2010US-00233, FIS2011-29493, Consolider CSD2010-00024 and CAM project and the Comunidad de Madrid No. S2009/MAT-1467 to P. J. P.; BFU2010-16382/BMC to C.S.M.; and FIS2011-16090-E to C.S.M. and P.J.P. S.J.F acknowledges funding from the National Institutes of Health, USA (GM037705 and AI1058172). A.J.P.-B. holds a Juan de la Cierva postdoctoral contract from the Ministry of Science and Innovation of Spain; A.O.-E. and R.M.-C. are recipients of predoctoral fellowships from the Ministry of Education and the Instituto de Salud Carlos III of Spain, respectivel

Linking Chronic Otitis Media and Nasal Obstruction: A CFD Approach

Objectives To investigate a possible relationship between altered nasal flow and chronic otitis media (COM) using computational fluid dynamics (CFD). Study Design Retrospective case series. Methods Retrospective cohort sample of CT scans from patients with COM and controls without COM to compare the results of various nasal airflow parameters determined by CFD between a group of patients with COM (N = 60) and a control group of subjects without any evidence of ear disease (N = 81). The CT were subjected to various procedures to carry out CFD studies, determining the resistance to nasal flow, the proportion of flow through the right and left nasal cavity, and two nondimensional estimators. The results of CFD studies between patients with COM and controls were compared. Results Whereas only 12.3% of the controls had CFD alteration (10 out of 81), 43.3% of the patients suffering COM displayed alterations of our nondimensional parameters urn:x-wiley:0023852X:media:lary29882:lary29882-math-0001 (26 out of 60). Conclusions According to our results, the incidence of alterations in nasal airflow by studying with CFD is significantly higher in patients with COM than in controls. To our knowledge, this is the first article linking nasal cavity and COM using a CFD approach. Our results support the hypothesis that nasal flow alterations could be implicated in the etiopathogenesis of the COM

Eliminating Error in the Chemical Abundance Scale for Extragalactic HII Regions

In an attempt to remove the systematic errors which have plagued the calibration of the HII region abundance sequence, we have theoretically modeled the extragalactic HII region sequence. We then used the theoretical spectra so generated in a double blind experiment to recover the chemical abundances using both the classical electron temperature + ionization correction factor technique, and the technique which depends on the use of strong emission lines (SELs) in the nebular spectrum to estimate the abundance of oxygen. We find a number of systematic trends, and we provide correction formulae which should remove systematic errors in the electron temperature + ionization correction factor technique. We also provide a critical evaluation of the various semi-empirical SEL techniques. Finally, we offer a scheme which should help to eliminate systematic errors in the SEL-derived chemical abundance scale for extragalactic HII regions.Comment: 24 pages, 9 Tables, 13 figures, accepted for publication in MNRAS. Updated considering minor changes during the final edition process and some few missing reference

Physical fitness and white matter microstructure in children with overweight or obesity: the ActiveBrains project

Recent studies investigated the association of cardiorespiratory fitness with white matter microstructure in children, yet little work has explored to what extent other components of physical fitness (i.e., muscular or motor fitness) are associated with white matter microstructure. Indeed, this association has not been previously explored in children with overweight/obesity who present a different white matter development. Therefore, we aimed to examine associations between physical fitness components and white matter microstructure in children with overweight/obesity. In total, 104 (10.04 +/- 1.15 years old; 43 girls) children were included in this cross-sectional study. Physical fitness was assessed using the ALPHA-fitness test battery. Fractional anisotropy (FA) and mean diffusivity were derived from diffusion tensor imaging (DTI). No association was found between physical fitness and global DTI metrics (all P>0.082). Within individual tracts, all associations became non-significant when analyses were adjusted for multiple comparisons. Using the voxel-wise approach, we identified a small cluster in the left lateral frontal lobe where children with greater upper-body muscular fitness showed higher FA (PFWE-corrected=0.042). Although our results cannot conclude physical fitness is related to white matter microstructure in children with overweight/obesity; those findings indicate that the association of muscular fitness with white matter microstructure might be more focal on frontal areas of the brain, as opposed to global differences

Besnoitiosis bovina: Estudio de los factores de riesgo asociados a la infección en una explotación del Pirineo aragonés (abstract)

La Besnoitiosis bovina es una enfermedad parasitaria de carácter reemergente causada por el protozoo Besnoitia besnoiti que en los últimos años ha experimentado un aumento en su prevalencia y una expansión geográfica en Europa (EFSA, 2010).Publishe

Educación y cooperación para el desarrollo mediante estándares universales de accesibilidad

II FORO «IBEROAMÉRICA EN LA ESCENA ECONÓMICA INTERNACIONAL: INVERSIONES Y EDUCACIÓN PARA EL DESARROLLO». UNIVERSIDAD REY JUAN CARLOS, MADRID (ESPAÑA), 14 DE DICIEMBRE DE 200