Beta-Lactamase Repressor BlaI Modulates Staphylococcus aureus Cathelicidin Antimicrobial Peptide Resistance and Virulence.

BlaI is a repressor of BlaZ, the beta-lactamase responsible for penicillin resistance in Staphylococcus aureus. Through screening a transposon library in S. aureus Newman for susceptibility to cathelicidin antimicrobial peptide, we discovered BlaI as a novel cathelicidin resistance factor. Additionally, through integrational mutagenesis in S. aureus Newman and MRSA Sanger 252 strains, we confirmed the role of BlaI in resistance to human and murine cathelidicin and showed that it contributes to virulence in human whole blood and murine infection models. We further demonstrated that BlaI could be a target for innate immune-based antimicrobial therapies; by removing BlaI through subinhibitory concentrations of 6-aminopenicillanic acid, we were able to sensitize S. aureus to LL-37 killing

Transitions to religious adulthood : relational geographies of youth, religion and international volunteering

This paper draws on data from an Arts and HumanitiesResearch Council and Economic and Social Research Council funded Religion and Society Youth Call project (AH/G016461/1).This paper offers important insights into the contemporary nature of youth transitions and the ways in which religious affiliation and engagement with international volunteering influences and interplays with negotiations of the life course. Situated within interdisciplinary debates about youth transitions, as well as discussions about relational geographies of age, religion and voluntarism, we demonstrate the multiple relationalities that are at play as young religious international volunteers negotiate the transition to religious adulthood. We focus on religious, vocational and aged relationalities to explore how these are engaged with, and experienced by, young people 'over there' in Latin America and 'back home' in the UK. Additionally, we explore how these relationalities shape identities within and between religiosity, age (youth) and volunteering, and how these are (re)organised through networks, flows and mobilities. To do so, we draw on our analysis of the experiences of young volunteers who participated in faith-based international volunteering with an evangelical Christian organisation that sends teams of young people on short-term missions to Latin America. We draw on our analyses of two sets of interviews with 22 young volunteers, 14 of whom also completed a diary about their experiences, supplemented by four focus groups. Our analysis points to the ways in which the spatial emphasis of geographical engagements with youth transitions can be well informed through considerations of religion; a key challenge being how young volunteers renegotiate their sense of religious self on returning 'back home'. In addition, although our participants displayed some self-reflexivity about the social construction of age, they did not necessarily exhibit an understanding of the power underpinning their encounters with others. Finally, our findings open up possibilities for appreciating the diversity of volunteering experiences and the role that volunteer organisations have in shaping the expectations and aspirations of those who participate in their programmes.Publisher PDFPeer reviewe

Diagnosis of latent tuberculosis infection is associated with reduced HIV viral load and lower risk for opportunistic infections in people living with HIV.

Approximately 28% of the human population have been exposed to Mycobacterium tuberculosis (MTB), with the overwhelming majority of infected individuals not developing disease (latent TB infection (LTBI)). While it is known that uncontrolled HIV infection is a major risk factor for the development of TB, the effect of underlying LTBI on HIV disease progression is less well characterized, in part because longitudinal data are lacking. We sorted all participants of the Swiss HIV Cohort Study (SHCS) with at least 1 documented MTB test into one of the 3 groups: MTB uninfected, LTBI, or active TB. To detect differences in the HIV set point viral load (SPVL), linear regression was used; the frequency of the most common opportunistic infections (OIs) in the SHCS between MTB uninfected patients, patients with LTBI, and patients with active TB were compared using logistic regression and time-to-event analyses. In adjusted models, we corrected for baseline demographic characteristics, i.e., HIV transmission risk group and gender, geographic region, year of HIV diagnosis, and CD4 nadir. A total of 13,943 SHCS patients had at least 1 MTB test documented, of whom 840 (6.0%) had LTBI and 770 (5.5%) developed active TB. Compared to MTB uninfected patients, LTBI was associated with a 0.24 decreased log HIV SPVL in the adjusted model (p < 0.0001). Patients with LTBI had lower odds of having candida stomatitis (adjusted odds ratio (OR) = 0.68, p = 0.0035) and oral hairy leukoplakia (adjusted OR = 0.67, p = 0.033) when compared to MTB uninfected patients. The association of LTBI with a reduced HIV set point virus load and fewer unrelated infections in HIV/TB coinfected patients suggests a more complex interaction between LTBI and HIV than previously assumed

The Lantern Vol. 39, No. 2, Spring 1973

• Days of Rain • Reflections On Clifton, New Jersey • Interlude • Window Scene • Eh! • Odyssey of Malcolm • Tuna on Toast • The Second Avenue Bus • Salutation of the Dawn • So Say Something • Mood • Moriarty\u27s Lament • I\u27ve Been a Lonely Gypsy • Change • Cool Ray • The Thinker • A Southern Sunsethttps://digitalcommons.ursinus.edu/lantern/1102/thumbnail.jp

Evaluating the effectiveness of road mitigation measures.

The last 20 years have seen a dramatic increase in efforts to mitigate the negative effects of roads and traffic on wildlife, including fencing to prevent wildlife- vehicle collisions and wildlife crossing structures to facilitate landscape connectivity. While not necessarily explicitly articulated, the fundamental drivers behind road mitigation are human safety, animal welfare, and/or wildlife conservation. Concomitant with the increased effort to mitigate has been a focus on evaluating road mitigation. So far, research has mainly focussed on assessing the use of wildlife crossing structures, demonstrating that a broad range of species use them. However, this research has done little to address the question of the effectiveness of crossing structures, because use of a wildlife crossing structure does not necessarily equate to its effectiveness. The paucity of studies directly examining the effectiveness of crossing structures is exacerbated by the fact that such studies are often poorly designed, which limits the level of inference that can be made. Without well performed evaluations of the effectiveness of road mitigation measures, we may endanger the viability of wildlife populations and inefficiently use financial resources by installing structures that are not as effective as we think they are. In this paper we outline the essential elements of a good experimental design for such assessments and prioritize the parameters to be measured. The framework we propose will facilitate col- laboration between road agencies and scientists to undertake research programs that fully evaluate effectiveness of road mitigation measures. We discuss the added value of road mitigation evaluations for policy makers and transportation agencies and provide recom- mendations on how to incorporate such evaluations in road planning practices

Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation

We present a new map depicting the first global biogeographic regionalization of Earth's freshwater systems. This map of freshwater ecoregions is based on the distributions and compositions of freshwater fish species and incorporates major ecological and evolutionary patterns. Covering virtually all freshwater habitats on Earth, this ecoregion map, together with associated species data, is a useful tool for underpinning global and regional conservation planning efforts (particularly to identify outstanding and imperiled freshwater systems); for serving as a logical framework for large-scale conservation strategies; and for providing a global-scale knowledge base for increasing freshwater biogeographic literacy. Preliminary data for fish species compiled by ecoregion reveal some previously unrecognized areas of high biodiversity, highlighting the benefit of looking at the world's freshwaters through a new framework.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Naturales y Muse

Freshwater ecoregions of the world: A new map of biogeographic units for freshwater biodiversity conservation

We present a new map depicting the first global biogeographic regionalization of Earth's freshwater systems. This map of freshwater ecoregions is based on the distributions and compositions of freshwater fish species and incorporates major ecological and evolutionary patterns. Covering virtually all freshwater habitats on Earth, this ecoregion map, together with associated species data, is a useful tool for underpinning global and regional conservation planning efforts (particularly to identify outstanding and imperiled freshwater systems); for serving as a logical framework for large-scale conservation strategies; and for providing a global-scale knowledge base for increasing freshwater biogeographic literacy. Preliminary data for fish species compiled by ecoregion reveal some previously unrecognized areas of high biodiversity, highlighting the benefit of looking at the world's freshwaters through a new framework.La lista completa de autores que integran el documento puede consultarse en el archivo.Facultad de Ciencias Naturales y Muse