Synthesis Of Structured Triacylglycerols Enriched In N-3 Fatty Acids By Immobilized Microbial Lipase

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The search for new biocatalysts has aroused great interest due to the variety of microorganisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 degrees C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine their effects on n-3 long-chain polyunsaturated fatty acids incorporation into soybean oil. Structured triacylglycerols with 11.7 and 7.2% of eicosapentaenoic acid + docosahexaenoic acid were obtained using Aspergillus niger lipase and Rhizopus javanicus lipase, decreasing the n-6/n-3 fatty acids ratio of soybean oil (11:1 to 3.5:1 and 4.7:1, respectively). The best reaction conditions were: initial water content of lipase of 0.86% (w/w), sardine-free faty acids:soybean oil mole ratio of 3:1 and reaction time of 36 h, at 40 degrees C. The significant factors for the acidolysis reaction were the sardine-free fatty acids:soybean oil mole ratio and reaction time. The characterization of structured triacylglycerols was obtained using easy ambient sonic-spray ionization mass spectrometry. The enzymatic reaction led to the formation of many structured triacylglycerols containing eicosapentaenoic acid, docosahexaenoic acid or both polyunsaturated fatty acids. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.47410061013Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Diretrizes para avaliação e tratamento fisioterapêutico da Síndrome de Pusher: estudo de caso

The Pusher´s Syndrome (PS) is a perceptual disorder that occurs in 10% of the cases of hemiparesis caused by stroke. It is characterized by falling to the paretic side, pushing to the paretic side with the non-paretic side and resisting to external correction. The present study aimed to describe the assessment, treatment and clinical evolution of a patient with left paresis and PS, caused by a stroke on the right hemisphere. Six months after the lesion, the patient was submitted to perceptual (human figure drawing test, behavioral inattention test, scale for contraversive pushing, minimental state examination) and functional performance tests (postural assessment stroke scale, Jebsen-Taylor hand function test, functional independence measure and Barthel index) and started physical therapy twice a week. Each session consisted of 3 parts of 20 minutes: sensory stimulation, motor training and sensory-motor integration. After six months, the patient showed improvement in all scales. The scale for contraversive pushing and the Jebsen-Taylor hand function test showed the highest percentages of improvement, 79% and 46%, respectively. Besides having been started six months after the lesion, the protocol of the present study contributed to the improvement of perception and functional performance. These findings suggest the importance of the physical therapy treatment in the recovery of individuals with PS.A Síndrome de Pusher (SP) é uma alteração perceptual presente em 10% dos casos de hemiparesia causada por Acidente Vascular Encefálico (AVE). Caracteriza-se por queda para o lado parético, comportamento de empurrar-se para o lado acometido com o hemicorpo não parético e resistência à correção externa. Este estudo visou descrever a avaliação, o tratamento e a evolução de uma paciente com hemiparesia à esquerda e SP, devido a um AVE no hemisfério cerebral direito. Seis meses após a lesão, a paciente realizou testes perceptuais (do desenho da figura humana, comportamental de inatenção, escala de avaliação do sintoma de empurrar, miniexame do estado mental) e de desempenho funcional (escala de avaliação postural para pacientes com AVE, teste de função manual de Jebsen-Taylor, medida de independência funcional e índice de Barthel) e iniciou fisioterapia duas vezes por semana. Cada sessão teve 3 partes de 20 minutos: estimulação sensorial, treino motor e integração sensório-motora. Após o tratamento a paciente foi reavaliada e apresentou melhora em todas as escalas. A de avaliação do sintoma de empurrar e o teste de função manual de Jebsen-Taylor foram os que registraram maiores percentuais de melhora, 79% e 46%, respectivamente. O protocolo utilizado, mesmo tendo sido iniciado seis meses após a lesão, proporcionou melhora perceptual e funcional, o que sugere a importância da fisioterapia na recuperação da SP

Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase

The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine their effects on n-3 long-chain polyunsaturated fatty acids incorporation into soybean oil. Structured triacylglycerols with 11.7 and 7.2% of eicosapentaenoic acid + docosahexaenoic acid were obtained using Aspergillus niger lipase and Rhizopus javanicus lipase, decreasing the n-6/n-3 fatty acids ratio of soybean oil (11:1 to 3.5:1 and 4.7:1, respectively). The best reaction conditions were: initial water content of lipase of 0.86% (w/w), sardine-free faty acids:soybean oil mole ratio of 3:1 and reaction time of 36 h, at 40 °C. The significant factors for the acidolysis reaction were the sardine-free fatty acids:soybean oil mole ratio and reaction time. The characterization of structured triacylglycerols was obtained using easy ambient sonic-spray ionization mass spectrometry. The enzymatic reaction led to the formation of many structured triacylglycerols containing eicosapentaenoic acid, docosahexaenoic acid or both polyunsaturated fatty acids.47410061013CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informaçã

Elaboration of an illustrated home exercise manual for stroke patients with hemiparesis

A realização de exercícios físicos para pacientes com sequelas motoras pós-acidente vascular encefálico (AVE) é essencial para a recuperação funcional. Programas educacionais podem facilitar a repetição dos exercícios em casa e contribuir para o tratamento. Este trabalho teve como objetivo elaborar e aplicar um manual de exercícios domiciliares para pacientes com AVE. O estudo consistiu em duas etapas: na primeira foi elaborado um manual de exercícios domiciliares (piloto) com fotos e textos simples para facilitar a compreensão. Foi realizada uma avaliação funcional de cada paciente e selecionados os exercícios mais adequados, que deveriam ser feitos em casa. Esse manual foi aplicado a 17 pacientes, 70% crônicos e 30% agudos. Após 15 dias, o paciente retornava e era solicitado que reproduzisse os exercícios e informasse o nível de compreensão das fotos e do texto do manual e se sentia dor ao realizá-los. As fotos e os exercícios referidos como difíceis foram revistos, e criada a versão teste que foi aplicada em outros 23 pacientes, dando origem à versão final do manual. A avaliação do manual piloto foi insatisfatória, sendo que apenas 56% referiram aprovação das fotos e 87% do texto. Após a reformulação, a compreensão das fotos e do texto alcançou valores acima de 98%. Foi possível obter um manual ilustrado de exercícios domiciliares, de fácil aplicação e compreensão, específico e individualizado, para pacientes com AVE e adaptável aos diferentes quadros motores

Encontrar filosofia(s) e infância(s)

Este texto trata de pensar en algunas posibilidades para unir "filosofía" e " infancia" en dos etapas: la primera es teórica y la segunda trae propuestas de trabajo en torno del tema. En la parte teórica, se presenta la propuesta de Matthew Lipman, que hizo posible llevar la filosofía a los niños. Inmediatamente, señalamos las contribuciones de otras áreas para pensar la infancia: en P. Ariès entendemos que el concepto de infancia no siempre ha existido; Postman nos dice que vivimos un proceso de adultización de los niños y de infantilización de los adultos; Clarice Cohn muestra, con su estudio de los Xikrin, que el concepto de la infancia puede no existir en algunas sociedades; Walter Kohan piensa el tiempo como intensidad (aión), y no sólo como la cronología (chrónos), refiriéndonos a una nueva mirada a la infancia; Larrosa trata de la alteridad de la infancia, y de la necesidad de entenderla como un rompecabezas. Por fin, nos fijamos en el niño como el que filosofa y mira la filosofía. En la segunda parte se presentan tres actividades (carta, fotos, poesía, pintura, y los fragmentos de Heráclito) propuestas como experiencias que hacen que sea posible discutir la relación entre filosofía e infancia.Este texto busca pensar algumas possibilidades de unir “filosofia” e “infância” em dois momentos: o primeiro é teórico e o segundo traz propostas de trabalho em torno da temática. Na parte teórica, apresentamos a proposta de Matthew Lipman que tornou possível levar a filosofia às crianças. Na sequência apontamos as contribuições de outras áreas para pensar a infância: em P. Ariès vemos que o conceito de infância nem sempre existiu; Postman nos indica que vivemos o processo de adultização das crianças e o de infantilização dos adultos; Clarice Cohn mostra, com seu estudo dos Xikrin, que o conceito de infância pode não existir em certas sociedades; Walter Kohan pensa o tempo como intensidade (aión) e não somente como cronologia (chrónos), nos remetendo a um novo olhar para a infância; Larrosa trata da alteridade da infância, e da necessidade de entendê-la como um enigma. Por fim, olhamos para a criança como aquela que filosofa e que olha para a filosofia. Na parte dois apresentamos três atividades (carta, fotos, poesia, pintura, e fragmentos de Heráclito) propostas como experiências que tornem possível problematizar as relações entre filosofia e infância.

Canine Hypothyroidism with Neurological Disorders

Background: Hypothyroidism is characterized by hypofunction of the thyroid gland. It results in deficient production of thyroid hormones. Neurological disorders resulting from hypothyroidism are rare, which highlights the importance of this study. This study reports a case of hypothyroidism in a dog with neurological clinical signs, that was treated at the Universidade Estadual de Santa Cruz’s Veterinary Hospital (HV-UESC).   Case: A 4-year-old male intact Dogo Argentino breed dog, weighing 64 kg, presenting obesity, anorexia, prostration, walking in circles, and chronic dermatopathy was presented at HV-UESC. Upon physical examination, the animal presented a deficit of proprioception in the 4 limbs, with preserved superficial and deep pain. No alteration was observed in the ears, that could explained the clinical signs. In terms of dermatopathy, the animal presented symmetrical alopecia in the lateral region of the thighs and tail. Blood samples were collected for a complete blood count and biochemical tests of urea, creatinine, ALT, AST and cholesterol. Imaging radiography and ultrasonography were performed, which ruled out thoracic and abdominal changes that could be related to the case. Prior to receiving the blood test results, idiopathic encephalitis was suspected and enrofloxacin and prednisone were prescribed for 7 days. During the medication period, previous exams were provided, which indicated only increased cholesterol (500 mg/dL). The animal showed no improvement with the prescribed medication. In view of the clinical signs presented by the patient and the results of the additional tests, hormonal disease was suspected, compatible with hypothyroidism. Thus, hormonal tests of total T4, free T4, and TSH were requested, leading to verification of reduced total T4 (0.3 ng/dL) and free T4 (0.15 ng/dL) levels, and confirming the dysfunction of the thyroid gland. The previous treatment was suspended and thyroid hormone replacement was initiated. After 3 days of treatment, the neurological signs regressed and the animal became more active; after 30 days, the areas of alopecia decreased. Although the patient did not receive the recommended clinical follow-up for such cases, it was possible to establish the ideal levothyroxine dosage for the dog after appropriate adjustments, which permitted thyroid hormone levels to return to normal. Discussion: This report refers to a case of hypothyroidism in a giant dog breed. The dog in the report showed clinical signs of a dermatological, metabolic, and neurological nature, which is consistent with a lack of thyroid hormone. The main signs presented by the animal were neurological, such as walking in circles and a deficit of proprioception in the four limbs. These clinical signs are rarely mentioned in the literature associated with hypothyroidism. Laboratory abnormalities are correlated with the severity and chronicity of the disease. The animal showed a decrease in total T4 and free T4, which is to be expected in a hypothyroid animal. As a result, levothyroxine replacement treatment was initiated. The dose used for the dog, which is the recommended dose in the literature, greatly increased its total T4 levels. As a result, the dose was readjusted after a new clinical evaluation. The rate of metabolism and absorption of levothyroxine varies widely and is independent of weight. The patient showed quickly improvement in neurological signs, activity level, and serum cholesterol rate. Regarding dermatological signs and body condition, there was a more gradual improvement. This corroborates what is mentioned in the literature, which indicates that improvements can take many months. Keywords:  hormonal Disease, hypothyroidism, neuropathy

Effects of Online Sensory Feedback Restriction during the Training on Immediate and Long-Term Learning on a Finger Opposition Tapping Task

Background: Online sensory feedback has been considered fundamental for motor learning. The sensory inputs experienced in previous attempts can be processed and compared to allow the online refinement of subsequent attempts, resulting on performance improvement. However, numerous studies have provided direct and indirect evidence that learning new motor abilities is possible, regardless of online sensory feedback. Objective: The purpose of this study was to investigate the influence of online sensory feedback on learning a new motor skill. Methods: We investigated the immediate and the 28-day delayed performance of a single-session training (4 blocks of 600 movements) performed under 4 different conditions of online sensory restriction: (1) no restriction; (2) visual input restriction; (3) visual and tactile input restriction and (4) total input restriction (mental practice), in 40 young healthy participants (mean age 26.6±3.6 years). The task consisted of performing finger opposition sequences using the dominant hand (right). Results: The training condition did not influence immediate or delayed performance in terms of accuracy or speed. Conclusion: As all conditions resulted in a similar amount of performance improvement, the level of online feedback available during training did not affect the learning process. Probably, according to the sensory inputs availability on the training conditions, equally efficient strategies are developed to improve performance in terms of speed and accuracy

A sazonalidade ambiental afeta a composição faunística de Ephemeroptera e Trichoptera em um riacho de Cerrado do Sudeste do Brasil?Does environmental seasonality affect the faunal composition of Ephemeroptera and Trichoptera in a Cerrado stream from

No presente trabalho, dados sobre Ephemeroptera e Trichoptera (ET) de um riacho de cerrado foram analisados, com o objetivo de testar a hipótese de que a alta sazonalidade neste bioma pode alterar a composição de ET entre as estações chuvosas e de seca. A estrutura da comunidade foi avaliada utilizando a Análise de Correspondência Destendenciada e a Análise de Agrupamento (Morisita-Horn, UPGMA). Os fatores ambientais foram submetidos à Análise de Componentes Principais. Para testar a influência das variáveis abióticas sobre a fauna, foram utilizados o Procrustean Randomization Test (Protest) e o Teste de Mantel. Os fatores ambientais registrados influenciaram significativamente a fauna de ET do Córrego do Pedregulho. A similaridade faunística foi alta ao longo do ano, indicando que, apesar de a densidade flutuar em resposta à variação da precipitação, a composição faunística apresentou baixa variabilidade temporal. Por outro lado, foi possível constatar que o gênero Lachlania (Ephemeroptera) ocorreu, preferencialmente, nos meses chuvosos e que a composição da fauna da estação seca variou menos do que aquela das demais estações. Portanto, a sazonalidade ambiental afetou parcialmente a composição da fauna de ET do Córrego do Pedregulho.Abstract In this paper, data on the fauna of Ephemeroptera and Trichoptera (ET) from a Cerrado stream was analysed in order to test the hypothesis that the high seasonality of this biome can influence the composition of ET between the wet and dry seasons. The community structure was evaluated using Detrended Correspondence Analysis and Cluster Analysis (Morisita Horn-UPGMA). Environmental factors were analyzed using the Principal Components Analysis. In order to test the effect of abiotic variables on the fauna, It was applied the Procrustean Randomization Test (Protest) and Mantel Test. The environmental factors recorded for this study had a significant effect on the ET fauna from Córrego do Pedregulho. Faunal similarity was high throughout the year, indicating that although there was density of fluctuation, due to rainfall variation, the faunal composition showed little temporal variability. On the other hand, it was possible to observe that the genus Lachlania (Ephemeroptera) occurred preferably during the rainy months and that the faunal composition during the dry season was less variable than those from other seasons. Therefore, environmental seasonality had a partial effect on the faunal composition of ET from Córrego do Pedregulho

Risk factors for falls in hospitalized adult patients: an integrative review

Objetivo: Identificar os fatores de risco para a ocorrência de quedas em pacientes adultos hospitalizados. Método: Revisão integrativa realizada nas bases de dados LILACS, SciELO, MEDLINE e Web of Science, abrangendo artigos publicados entre 1989 e 2012. Resultados: Setenta e um artigos compuseram a amostra final do estudo. Os fatores de risco para quedas apresentados nesta revisão foram relacionados ao paciente (intrínsecos), ao ambiente hospitalar e ao processo de trabalho dos profissionais da saúde, em especial à enfermagem (extrínsecos). Conclusão: A triagem sistemática de fatores de risco para queda foi identificada como fator contribuinte para a redução desse agravo, auxiliando a não ocorrência deste evento que, apesar de ser prevenível, pode determinar consequências graves incluindo o óbito.

Objective: Identifying risk factors for the occurrence of falls in hospitalized adult patients. Method: Integrative review carried out in the databases of LILACS, SciELO, MEDLINE and Web of Science, including articles published between 1989 and 2012. Results: Seventy-one articles were included in the final sample. Risk factors for falls presented in this review were related to patients (intrinsic), the hospital setting and the working process of health professionals, especially in nursing (extrinsic). Conclusion: The systematic screening of risk factors for falls was identified as a contributing factor to the reduction of this injury, helping the non-occurrence of this event that, despite being preventable, can have serious consequences including death.
Objetivo: Identificar los factores de riesgo para la ocurrencia de caídas en pacientes adultos hospitalizados. Método: Revisión integradora de la literatura de artículos publicados entre los años 1989 al 2012 en las bases de datos LILACS, SciElO, MEDLINE y Web of Science. Resultados: La muestra final estuvo compuesta por setenta y un artículos. Entre los factores de riesgo de caídas indicados en esta revisión están los relacionados con el paciente (intrínsecos), con el ambiente hospitalario y con el proceso de trabajo de los profesionales de la salud, especialmente enfermería (extrínsecos). Conclusión: La detección sistemática de factores de riesgo asociados a caídas fue identificada como un factor que contribuye a la reducción de este daño, favoreciendo de esta manera su no ocurrencia, la que a pesar de ser prevenible puede acarrear consecuencias graves, incluyendo la muerte.