32 research outputs found
肝細胞癌におけるPIVKA-II測定の意義: PIVKA-II微量測定を含めた検討
取得学位 : 博士(医学), 学位授与番号 : 医博乙第1210号, 学位授与年月日:平成5年2月17日,学位授与年:199
Development of a Novel Method for in vivo Determination of Activation Energy of Glucose Transport Across S. cerevisiae Cellular Membranes. A Biosensor-like Approach
Whereas biosensors have been usually proposed as analytical tools, used to investigate the surrounding media pursuing an analytical answer, we have used a biosensor-like device to characterize the microbial cells immobilized on it. We have studied the kinetics of transport and degradation of glucose at different concentrations and temperatures. When glucose concentrations of 15 and 1.5 mM were assayed, calculated activation energies were 25.2 and 18.4 kcal mol−1, respectively, in good agreement with previously published data. The opportunity and convenience of using Arrhenius plots to estimate the activation energy in metabolic-related processes is also discussed
Cell membrane damage induced by phenolic acids on wine lactic acid bacteria
The aim of this work was to investigate the effect of phenolic acids on cell membrane permeability of
lactic acid bacteria from wine. Several phenolic acids were tested for their effects on the cell membrane of
Oenococcus oeni and Lactobacillus hilgardii by measuring potassium and phosphate efflux, proton influx and
by assessing culture viability employing a fluorescence technique based on membrane integrity. The
experimental results indicate that hydroxycinnamic acids (p-coumaric, caffeic and ferulic acids) induce
greater ion leakages and higher proton influx than hydroxybenzoic acids (p-hydroxibenzoic, protocatechuic,
gallic, vanillic, and syringic acids). Among the hydroxycinnamic acids, p-coumaric acid showed the strongest
effect. Moreover, the exposure of cells to phenolic acids caused a significant decrease in cell culture viability,
as measured by the fluorescence assay, in both tested strains. The results agree with previous results
obtained in growth experiments with the same strains. Generally, phenolic acids increased the cell
membrane permeability in lactic acid bacteria from wine. The different effects of phenolic acids on
membrane permeability could be related to differences in their structure and lipophilic character