An enhanced platform for cell electroporation: controlled delivery and electrodes functionalization

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The transcription factor BATF operates as an essential differentiation checkpoint in early effector CD8+ T cells

The transcription factor BATF is required for interleukin 17 (IL-17)-producing helper T cell (TH17) and follicular helper T cell (TFH) differentiation. Here, we show that BATF also has a fundamental role in regulating effector CD8+ T cell differentiation. BATF-deficient CD8+ T cells show profound defects in effector expansion and undergo proliferative and metabolic catastrophe early after antigen encounter. BATF, together with IRF4 and Jun proteins, binds to and promotes early expression of genes encoding lineage-specific transcription-factors (T-bet and Blimp-1) and cytokine receptors, while paradoxically repressing genes encoding effector molecules (IFN-γ and granzyme B). Thus, BATF amplifies TCR-dependent transcription factor expression and augments inflammatory signal propagation but restrains effector gene expression. This checkpoint prevents irreversible commitment to an effector fate until a critical threshold of downstream transcriptional activity has been achieved

Turfgrass research report 1996

Preemergence herbicide efficacy on crabgrass-1996 / J. Street and R. Stewart --Postemergence herbicide efficacy on crabgrass -1996 / J. Street and R. Stewart -- General turfgrass broadleaf weed control evaluation / W. Pound -- Nonselective herbicide evaluation / W. Pound -- Postemergent yellow nutsedge evaluation / W. Pound and R. Stewart -- Preemergent broadleaf weed control / W. Pound -- Control of ant mounds in turfgrass-1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Control of hairy chinch bugs in turfgrass -1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Preventive and curative insecticide applications for control of hairy chinch bugs in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Quick kill of black cutworm larvae in bentgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Control of black cutworm, Agrotis ipsilon (Hufnagel), and sod webworms (Pyralidae: Crambinae) in creeping bentgrass, Agrostis palustris Hudson, with spinosad formulations, Columbus, Ohio, 1996 / D. J. Shetlar, H. D. Niemczyk and M. Belcher -- Control of black cutworm, Agrotis ipsilon (Hufnagel), and sod webworms (Pyralidae: Crambinae) in creeping bentgrass, Agrostis palustris Hudson, with observations on black turfgrass Ataenius adults, Ataenius spretulus (Haldeman), Columbus, Ohio, 1996 / D. J. Shetlar, H. D. Niemczyk and M. Belcher -- Control of black cutworm, Agrotis ipsilon (Hufnagel), sod webworms (Pyralidae: Crambinae), and black turfgrass Ataenius adults, Ataenius spretulus (Haldeman), in creeping bentgrass, Agrostis palustris Hudson, Columbus, Ohio, 1996 / D. J. Shetlar, H. D. Niemczyk and M. Belcher -- Control of bluegrass billbug, Sphenophorus parvulus Gyllenhal, larvae in lawn turf, Wooster, Ohio, 1996 / D. J. Shetlar, H. D. Niemczyk and M. Belcher -- Efficacy of spinosad applied prior to oviposition for control of bluegrass billbug larvae in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Efficacy of insecticides applied prior to oviposition for control of bluegrass billbug larvae in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Application of insecticides to kill black turfgrass Ataenius adults prior to egg laying on golf course fairways-1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Preventive applications for control of Japanese beetle larvae in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Influence of application date on the efficacy of insecticides applied for control of Japanese beetle larvae in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Application of insecticides for preventive control of Japanese beetle larval populations in turfgrass - 1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Application of Bacillus thuringiensis strain 'buibui' for control of white grubs in turfgrass -1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- Curative control of masked chafer larvae in turfgrass -1996 / D. J. Shetlar, H. D. Niemczyk and K. T. Power -- A field test of RH-0345 2SC and 2.5G at 2.0 lb.ai/ acre and Merit 75WP at 0.3 lb. ai/ acre for control of black cutworm larvae on golf course greens / H. D. Niemczyk -- Dollar spot control study - 1996 / K. Danneberger, J. Rimelspach, M. Boehm, and J. Taylor -- quality ratings for various fungicide treatments on creeping bentgrass putting green turf / K. Danneberger, J. Rimelspach, M. Boehm, and J. Taylor -- Brown patch control study on creeping bentgrass turf - 1996 / K. Danneberger, J. Rimelspach, M. Boehm, and J. Taylor -- Brown patch control on tall fescue / K. Danneberger, J. Rimelspach, M. Boehm, and J. Taylor -- Yellow tuft study-1996 / K. Danneberger, J. Rimelspach, M. Boehm, and J. Taylor -- Creeping bentgrass melting-out study / K. Danneberger, J. Taylor, R. Golembiewski, G. Bell, J. Rimelspach, and M. Boehm -- Kentucky bluegrass melting-out study / K. Danneberger, J. Rimelspach, M. Boehm and J. Taylor -- Red thread control study on perennial ryegrass / J. Rimelspach, K. Danneberger, and M. Boehm -- Red thread control study on Kentucky bluegrass / J. Rimelspach, K. Danneberger and M. Boehm -- Evaluation of fungicides for the control of red thread in Kentucky bluegrass, 1996 / J. Rimelspach, M. Boehm, K. Danneberger and J. Taylor -- Evaluation of fungicides for the control of red thread in perennial ryegrass, 1996 / J. Rimelspach, M. Boehm, K. Danneberger and J. Taylor -- Pink snow mold control study-1995-1996 / J. Rimelspach and K. Danneberger -- Primo & Sentinel applications on Poa annua quality / K. Danneberger, J. Taylor, R. Golembiewski, and G. Bell -- Nitrogen source, rate, and timing effect on Kentucky bluegrass -1996 / J. R. Street and R. M. Stewart -- Natural organic source evaluation on a Kentucky bluegrass-perennial ryegrass mixture-1996 / J. R. Street and R. M. Stewart -- Polymer-coated urea source and rate effect on Kentucky bluegrass / J. R. Street and R. M. Stewart -- Polymer-coated urea and IBDU fertilizer performance on Kentucky bluegrass / J. R. Street and R. M. Stewart -- 1993 NTEP bentgrass (fairway/tee) cultivar evaluation / J. A. Taylor -- 1993 NTEP fineleaf fescue cultivar evaluation / J. Taylor -- 1994 NTEP perennial ryegrass cultivar evaluation / J. Taylor -- 1995 Kentucky bluegrass (medium/high input) cultivar evaluation / J. Taylor -- 1995 NTEP Kentucky bluegrass (low input) cultivar evaluation / J. Taylor -- Controlling annual bluegrass and rough bluegrass in creeping bentgrass fairways: a nutritional approach / G. E. Bell, E. Odorizzi and T. K. Danneberger -- "Primo" growth regulator evaluation on creeping bentgrass / W. Pound and R. Stewart -- Influence of dollar spot on a blend of two creeping bentgrass cultivars / R. C. Golembiewski, T. K. Danneberger and P. M. Sweeney -- Dollar spot severity as influenced by Primo, creeping bentgrass cultivars, and nitrogen fertility / R. C. Golembiewski and T. K. Danneberger -- Identification of bulk samples of perennial ryegrass cultivars with RAPD Markers / P. M. Sweeney and T. K. Danneberge

Polyfunctional T cell responses in children in early stages of chronic Trypanosoma cruzi infection contrast with monofunctional responses of long-term infected adults

Background: Adults with chronic Trypanosoma cruzi exhibit a poorly functional T cell compartment, characterized by monofunctional (IFN-γ-only secreting) parasite-specific T cells and increased levels of terminally differentiated T cells. It is possible that persistent infection and/or sustained exposure to parasites antigens may lead to a progressive loss of function of the immune T cells. Methodology/Principal Findings: To test this hypothesis, the quality and magnitude of T. cruzi-specific T cell responses were evaluated in T. cruzi-infected children and compared with long-term T. cruzi-infected adults with no evidence of heart failure. The phenotype of CD4+ T cells was also assessed in T. cruzi-infected children and uninfected controls. Simultaneous secretion of IFN-γ and IL-2 measured by ELISPOT assays in response to T. cruzi antigens was prevalent among T. cruzi-infected children. Flow cytometric analysis of co-expression profiles of CD4+ T cells with the ability to produce IFN-γ, TNF-α, or to express the co-stimulatory molecule CD154 in response to T. cruzi showed polyfunctional T cell responses in most T. cruzi-infected children. Monofunctional T cell responses and an absence of CD4+TNF-α+-secreting T cells were observed in T. cruzi-infected adults. A relatively high degree of activation and differentiation of CD4+ T cells was evident in T. cruzi-infected children. Conclusions/Significance: Our observations are compatible with our initial hypothesis that persistent T. cruzi infection promotes eventual exhaustion of immune system, which might contribute to disease progression in long-term infected subjects.Fil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: de Rissio, Ana María. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Tomas, Gonzalo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Serjan, Alicia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Juan A. Fernández"; ArgentinaFil: Alvarez, María Gabriela. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Viotti, Rodolfo Jorge. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Fichera, Laura Edith. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esteva, Mónica Inés. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Potente, Daniel Fernando. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Armenti, Alejandro. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Tarleton, Rick L.. University of Georgia; Estados UnidosFil: Laucella, Susana Adriana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Functionalized microelectrodes arrays with integrated microfluidic channels for single-site multiple

Nowadays, different chemical and physical transfection techniques are used to delivery biomolecules of interest (e.g. DNA, RNA, proteins) into cells. Among the physical methods, electroporation generates transient pores in the plasma membrane by applying electrical pulses to suspended cells. One of its main limitations is the lack of spatio-temporal control over the process: it does not allow to select single cells (desirable requirement especially in highly heterogeneous tissues), and to monitor the transfection results in real-time. To circumvent these disadvantages, alternative microscale approaches are increasingly required. This work presents an integrated platform consisting of a gold microelectrode array (MEA) for singlesite electroporation and fluidic channels for controlled delivery of bio-chemical entities . In order to improve the efficiency of electroporation, the gold electrodes were coated with a thin film of nanostructured of Titanium Dioxide

Regulation of human intestinal T-cell responses by type 1 interferon-STAT1 signaling is disrupted in inflammatory bowel disease

This work was supported by a research fellowship grant from the Crohn’s and Colitis in Childhood Research Association (CICRA) and a small project grant from Crohn’s and Colitis UK (CCUK). We would like to acknowledge Professor Ian Sanderson, who helped with the initial design of this work, and provided important support throughout. We would also like to thank Dr Gary Warne for his advice and assistance in the use of the sorting by flow cytometry. We would also like to thank Dr Raj Lahiri and Professor Graham Foster for the kind gift of the primers for the ISGs (2’5’ OAS and MxA)

SISTEMA DE COMPRAS IFC

O trabalho apresentado na disciplina de Extensão e Pesquisa em Computação no Cursode Bacharelado em Ciências da Computação- trata de uma solução para o setor decompras do Instituto Federal Catarinense cuja necessidade surgiu da atual dinâmica noprocesso de compras de materiais, por exemplo, do IFC. Essas compras são feitas apartir do preenchimento manual penoso e exaustivo de diversos formulários quedemanda tempo e é passível de erros. Com o propósito de estabelecer um procedimentomais rápido, confiável e simplificado, o projeto tem como objetivo desenvolver umsistema capaz de automatizar essas tarefas e informatizar integralmente o processo. Essaabordagem também proporcionará vantagens adicionais, como a maior transparêncianas operações e o aumento na confiabilidade dos dados. Dando importância àcomplexidade e à profundidade dessa metodologia falha do setor de compras doInstituto, tornou-se um propósito apresentar um programa de software comaprofundamento das linguagens de programação, bem como, na construção de bases dedados como meio para armazenamento de informações. Dentre as tecnologias utilizadas, estarão: PHP, HTML, CSS, Javascript e MYSQL. Cabe destacar, ainda, que parafacilitar os afazeres do projeto, foram utilizadas metodologias ágeis de organizaçãocomo a plataforma Notion, cuja eficiência permitiu a distribuição efetiva de tarefas paraque o presente projeto fosse finalizado e, desse modo, a potencialização dos resultadosesperados. Cabe inferir, portanto, que a presente proposta destaca a importância de umaperspectiva sistêmica e atualizada da agenda de compras do IFC. Através dadisponibilização periódica de relatórios abrangentes, o sistema proporcionará melhoriassignificativas nas tomadas de decisões e no gerenciamento de processos. Assim, ficaevidente o quanto o trabalho descrito é necessário dentro do setor de compras além debeneficiar todos os envolvidos no processo e seus receptores, os estudantes e osprofessores. Espera-se auxiliar no crescimento do ambiente estudantil do Instituto paraque esse seja cada vez mais um exemplo de educandário

PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death

Neuronal Ca levels are regulated by glutamate receptor activation, which recruits PIKfyve to voltage-gated Ca channels, prompting their degradation

Sex Disparity in Cord Blood FoxP3+ CD4 T Regulatory Cells in Infants Exposed to Malaria In Utero.

Sex differences in the immune response and in infectious disease susceptibility have been well described, although the mechanisms underlying these differences remain incompletely understood. We evaluated the frequency of cord blood CD4 T cell subsets in a highly malaria-exposed birth cohort of mother-infant pairs in Uganda by sex. We found that frequencies of cord blood regulatory T cell ([Treg] CD4+CD25+FoxP3+CD127lo/-) differed by infant sex, with significantly lower frequencies of Tregs in female than in male neonates (P = .006). When stratified by in utero malaria exposure status, this difference was observed in the exposed, but not in the unexposed infants