Sperm velocity in a promiscuous bird across experimental media of different viscosities

Schmoll T, Rudolfsen G, Schielzeth H, Kleven O. Sperm velocity in a promiscuous bird across experimental media of different viscosities. Proceedings of the Royal Society B: Biological Sciences. 2020;287(1931): 20201031.In species with internal fertilization, the female genital tract appears challenging to sperm, possibly resulting from selection on for example ovarian fluid to control sperm behaviour and, ultimately, fertilization. Few studies, however, have examined the effects of swimming media viscosities on sperm performance. We quantified effects of media viscosities on sperm velocity in promiscuous willow warblers Phylloscopus trochilus. We used both a reaction norm and a character-state approach to model phenotypic plasticity of sperm behaviour across three experimental media of different viscosities. Compared with a standard medium (Dulbecco's Modified Eagle Medium, DMEM), media enriched with 1% or 2% w/v methyl cellulose decreased sperm velocity by up to about 50%. Spermatozoa from experimental ejaculates of different males responded similarly to different viscosities, and a lack of covariance between elevations and slopes of individual velocity-by-viscosity reaction norms indicated that spermatozoa from high- and low-velocity ejaculates were slowed down by a similar degree when confronted with high-viscosity environments. Positive cross-environment (1% versus 2% cellulose) covariances of sperm velocity under the character-state approach suggested that sperm performance represents a transitive trait, with rank order of individual ejaculates maintained when expressed against different environmental backgrounds. Importantly, however, a lack of significant covariances in sperm velocity involving a cellulose concentration of 0% indicated that pure DMEM represented a qualitatively different environment, questioning the validity of this widely used standard medium for assaying sperm performance. Enriching sperm environments along ecologically relevant gradients prior to assessing sperm performance will strengthen explanatory power of in vitro studies of sperm behaviour

DNA i ruggfjädrar visar tajgasädgåsens Anser f. fabalis ortstrohet till ruggningslokaler i Lappland

Recently, the Taiga Bean Goose Anser f. fabalis breeding population of Southern Lapland, Sweden, was shown to use a network of local sites during their summer wing moult. We used microsatellite markers to identify individual geese from DNA in shed feathers collected in 2016–2020 on six sites, enabling us to identify 168 unique individuals from 178 fully genotyped feathers. Nine individuals were represented multiple times among the collected feathers. All controls of identified individuals were made on the original site, never on an alternative site. Our results suggest a significant level of site fidelity and, thus, the need to provide a stable, low-disturbance network of moulting sites for the Southern Lapland sub-population of this endangered taxon. moult, site fidelity, conservation, genetics, population structureDNA profiles of shed Taiga Bean Goose feathers indicate between-season fidelity to moulting sites in Swedish LaplandpublishedVersio

Migratory patterns of Ospreys (Pandion haliaetus) from central Norway

Migratory behaviour is subject to intraspecific variation and may be determined by the age, sex and population origin of individuals. Here we equipped eight juvenile Ospreys (Pandion haliaetus) with GPS-based satellite-transmitters to investigate their migratory behaviour from a recently re-established breeding area in central Norway. Migration routes fanned out widely across Europe, and migratory behaviour differed between individuals. Five of the eight Ospreys completed their first southward migration to wintering areas in tropical Africa. They travelled a median distance of 7,482 km and spent from 21 to 92 days on their journeys. Median travel distance was 120% longer than a straight-line distance. Stopover sites were located just before or immediately after crossings of geographical barriers and the differences in the time spent on the migratory journeys was mainly a consequence of the number of stopover days. Only two individuals gave signals long enough to enable us to track their first northward migration in their third calendar year. By including repeated migratory journeys for these two individuals we tracked a total of 20 sea crossings lasting between 4 and 24 hours, and 14 trans-Saharan journeys. Four of the sea crossings included periods of nocturnal flight. The duration of the trans-Saharan journeys varied from 130 to 276 hours and the birds showed a strict diurnal rhythm, with movements only during daytime. Repeated migratory journeys showed that the Ospreys were faithful to wintering and stopover sites. Our results show that Ospreys from central Norway migrate on a broad front over Europe. However, the majority of the juvenile Ospreys followed flyways through central and eastern Europe and finally settled down in wintering areas in eastern parts of western tropical Africa.publishedVersio

Extra-pair paternity and sperm length variation in a far northern Great Tit (Parus major) population

Extra-pair paternity is common among socially monogamous bird species and considered an important driver of post-copulatory sexual selection on ejaculate traits including sperm traits. Patterns of extra-pair paternity and sperm size both show substantial variation among populations, yet we know little about the expression of these key reproductive traits at high latitudes. Here we report patterns of extra-pair paternity and describe variation in sperm dimensions in a Norwegian population of the socially monogamous Great Tit (Parus major) breeding beyond the polar circle at 69° northern latitude. Across six study years, we detected extra-pair paternity in 19.2% of 26 broods, and on average 4.7% of nestlings per brood were extra-pair offspring. As expected from results of previous intraspecific analyses of latitudinal variation in extra-pair paternity rates, the observed rate of extra-pair offspring was low in comparison to published estimates from more southern Great Tit populations (range: 2.9 − 20.4%). Our results therefore support a pattern of decreasing levels of extra-pair paternity with increasing latitude in this species also for extremely high latitudes. Overall mean sperm total length amounted to 97.5 ± 0.6 (SE) μm and 30.6% of the total phenotypic variation in sperm total length was explained by differences among sperm samples. The among-sample coefficient of variation in mean sperm total length per sample was 1.93%. Using previous comparative work as a yardstick, this value is substantially lower than expected for the observed frequency of 4.7% extra-pair offspring. Extra-pair copulation · Latitudinal variation · Passerine · Promiscuity · Social monogamy · Sperm morphologyExtra-pair paternity and sperm length variation in a far northern Great Tit (Parus major) populationpublishedVersio

Lynx gene flow in Fennoscandia

This study assesses the connectivity and gene flow between Eurasian lynx (Lynx lynx) populations in Finland and Scandinavia for the purpose of providing data for the evaluation of favorable conservation status for the European Union Habitats Directive. Using tissue samples from deceased lynx in Fennoscandia, i.e., Sweden, Norway, and Finland, collected between 2019 and 2022, we genotyped these samples using 91 single nucleotide polymorphisms (SNPs). A separate data set was used for validation, consisting of 73 samples from Scandinavia collected between 2010 and 2015 that were RAD sequenced and genotyped using 881 SNPs. We assessed genetic structure within all of Fennoscandia, as well as genetic differentiation and recent gene flow between Scandinavia (Sweden, Norway) and Finland. We also estimated individual relatedness to identify first order relatives, or immediate family members, for the purpose of assessing contemporary connectivity. The results suggest distinct genetic differentiation between the Scandinavian and Finnish lynx populations, with migration rates of approximately eight migrants per generation in either direction, which is comparably low but likely sufficient to prevent complete genetic isolation. These findings have significant implications for lynx conservation strategies under the European Habitats Directive. They underscore the importance of maintaining genetic diversity and facilitating connectivity between transboundary populations, highlighting the need for collaborative management approaches between Sweden, Norway, and Finland

Expanding the spatial scale in DNA-based monitoring schemes: ascertainment bias in transnational assessments

Harmonising methodology between countries is crucial in transborder population monitoring. However, immediate application of alleged, established DNA-based methods across the extended area can entail drawbacks and may lead to biases. Therefore, genetic methods need to be tested across the whole area before being deployed. Around 4,500 brown bears (Ursus arctos) live in Norway, Sweden, and Finland and they are divided into the western (Scandinavian) and eastern (Karelian) population. Both populations have recovered and are connected via asymmetric migration. DNA-based population monitoring in Norway and Sweden uses the same set of genetic markers. With Finland aiming to implement monitoring, we tested the available SNP-panel developed to assess brown bears in Norway and Sweden, on tissue samples from a representative set of 93 legally harvested individuals from Finland. The aim was to test for ascertainment bias and evaluate its suitability for DNA-based transnational-monitoring covering all three countries. We compared results to the performance of microsatellite genotypes of the same individuals in Finland and against SNP-genotypes from individuals sampled in Sweden (N = 95) and Norway (N = 27). In Finland, a higher resolution for individual identification was obtained for SNPs (PI = 1.18E-27) compared to microsatellites (PI = 4.2E-11). Compared to Norway and Sweden, probability of identity of the SNP-panel was slightly higher and expected heterozygosity lower in Finland indicating ascertainment bias. Yet, our evaluation show that the available SNP-panel outperforms the microsatellite panel currently applied in Norway and Sweden. The SNP-panel represents a powerful tool that could aid improving transnational DNA-based monitoring of brown bears across these three countries. DNA-based transborder monitoring · Genetic methods · Microsatellites · Noninvasive sampling · Singlenucleotide polymorphism · Wildlife managementpublishedVersio

No Evidence for Pre-Copulatory Sexual Selection on Sperm Length in a Passerine Bird

There is growing evidence that post-copulatory sexual selection, mediated by sperm competition, influences the evolution of sperm phenotypes. Evidence for pre-copulatory sexual selection effects on sperm traits, on the other hand, is rather scarce. A recent paper on the pied flycatcher, Ficedula hypoleuca, reported phenotypic associations between sperm length and two sexually selected male traits, i.e. plumage colour and arrival date, thus invoking pre-copulatory sexual selection for longer sperm. We were unable to replicate these associations with a larger data set from the same and two additional study populations; sperm length was not significantly related to either male plumage colour or arrival date. Furthermore, there was no significant difference in sperm length between populations despite marked differences in male plumage colour. We also found some evidence against the previously held assumption of longer sperm being qualitatively superior; longer sperm swam at the same speed as shorter sperm, but were less able to maintain speed over time. We argue that both empirical evidence and theoretical considerations suggest that the evolution of sperm morphology is not primarily associated with pre-copulatory sexual selection on male secondary sexual traits in this or other passerine bird species. The relatively large between-male variation in sperm length in this species is probably due to relaxed post-copulatory sexual selection

Resident bird species track inter-annual variation in spring phenology better than long-distance migrants in a subalpine habitat

The ability to track variation in climate is important for species to persist in a given environment. Lack of responses to both long-term changes and inter-annual variation in climate parameters can result in reduced fitness and population decline. Furthermore, migration strategy can influence the ability to track climatic variation due to the potential to use reliable environmental cues. Here, we studied the temporal relationship between birch leafing and onset of breeding for three bird species with contrasting migration strategies over a 20-year period in a subalpine habitat in Central Norway. We found no temporal change in birch leafing date or breeding onset for the three bird species over the study period. However, we found a statistically significant difference in the ability to track inter-annual variation in birch leafing date between the resident and two long-distance migratory species. The resident great tit Parus major was more capable of initiating egg laying in closer association to variation in birch leafing in early springs, than the long-distance migratory European pied flycatcher Ficedula hypoleuca and common redstart Phoenicurus phoenicurus. Long-distance migrants seem to have been constrained by arrival date or time from arrival to entering the breeding areas, in contrast to resident birds, which might be better able track early initiation of spring in breeding areas by adjusting egg laying date. Our findings highlight the importance of not solely studying directional long-term climatic change, but also pay attention to inter-annual variation

Effects of satellite transmitters on survival in Snowy Owls Bubo scandiacus

The use of tracking devices to monitor birds is extensive, but the effects of such instruments on equipped individuals are still insufficiently taken into account. Here we evaluate potential effects of backpack-mounted satellite transmitters (platform terminal transmitters; PTTs) on survival of 28 Snowy Owls Bubo scandiacus. Six confirmed deaths were all probably related to natural and human-induced causes. Although PTT operational time was significantly shorter than expected lifetime of Snowy Owls, five owls were observed alive after transmissions ceased. Additionally four PTTs stopped due to low battery levels, indicating end of transmitter life and not owl mortality. We found no evidence of mortality caused by PTTs, but sample sizes are relatively low and detrimental effects on equipped Snowy Owls cannot be excluded. We recommend caution when instrumenting large owls.publishedVersionNivå

Long-term genetic monitoring of a reintroduced Eurasian lynx population does not indicate an ongoing loss of genetic diversity

Where reintroduced wildlife populations are considered as vulnerable this is generally due to limited founder size and isolation. While many of these populations show low levels of genetic diversity, little is known about the temporal patterns of genetic diversity loss and the role of initial founder effects vs. ongoing genetic drift. Here we analysed genotype data from 582 Eurasian lynx samples from the reintroduced Bohemian-Bavarian-Austrian population (BBA) over a time span of 35 years, representing approximately 13 generations. Two-wave reintroduction of lynx from at least two distinct West-Carpathian areas resulted in relatively high start-up of genetic diversity. After the initial decline when the population lost about a quarter of its genetic diversity compared to the Carpathian source population, the genetic diversity and effective population size remained almost unchanged over the next 20 years. Despite confirmed isolation of BBA and thus absence of gene flow, we detected relatively low inbreeding during the two recent decades within the slightly increasing population size, which may have prevented ongoing loss of genetic diversity. Given the current status of BBA, we do not support genetic reinforcement to maintain its long-term viability; but urge the importance of facilitating gene flow with neighbouring lynx populations through an improvement of landscape connectivity and by strengthening law enforcement as well as the prevention of illegal killings. A sound genetic monitoring alongside regular camera trap-based monitoring of population size, health status and reproduction is pivotal to decide on future conservation interventions.publishedVersio