Morpho-functional basis of endothelial dysfunction in diabetes mellitus

The diabetes mellitus (DM) inevitably progresses and leads to complications, among which the main place is occupied by micro- and macroangiopathies. The presence of endothelial damage in DM can be established even before macroscopically significant damage to the vessel. At the same time, there is no summary ED characteristic for diabetes. The aim of the study is to make a comprehensive evaluation of ED in DM -1 and DM-2 types.Materials and methods of research. 60 persons, including 53 DM type 1 and type 2, with a severe course (state of decompensation) participated in the present study. We used the method of estimating ED by the number of circulating desquamation endothelial cells (CECs) at the stages of decomposition with simultaneous determination of NO2- and NO3- metabolites of nitric oxide.Results and discussion. In patients with diabetes, the level of CECs increased in 3-5 times and ranged from 1800 to 11,200 cells / ml. The average amount of CECs in patients with diabetes was 3358.5 ± 366.3 cells / ml.Conclusions: Endothelium is involved in the pathological process at DM. This is evidenced by a significant increase in CECs in the blood plasma. The use of this method allows to detect ED before clinically considerable vascular impairment and reflects the severity of the course and duration of DM

The Determination of Metals in Welding Fume by X-Ray-Spectrometry

Analysis of the current hygienic situation in the welding production showed that the intensification of welding processes involves the deterioration of air quality, which negatively affects the welders health. Respiratory effects seen in full-time welders have included bronchitis, airway irritation, lung function changes, and a possible increase in the incidence of lung cancer. The metal concentration in the air of the working area have been determined using the photometric method of analysis, which involves the stage of decomposition of the sample material before analysis. However, losses of the analyzed elements are possible when the sample is decomposed. The X-ray fluorescence method of analysis has the advantage of being nondestructive. The investigations shown the data of photometric determination of metals in welding aerosols is 1.5(-:)2 times lower than the results of X-ray fluorescence analysis

Research of ozonation process of the biologically pre-purified municipal wastewater

The paper considers the problem of post-treatment of municipal wastewater in Odessa using ozone. A significant place is given to the effect of the breakdown of toxic substances and important carcinogens. Based on theoretical studies, it was concluded that biological wastewater treatment does not guarantee a significant effect of the neutralization of many toxicants. The authors hypothesize the significant advantage of ozonation for the disposal and disinfection of wastewater compared to other methods, for example, chlorination. An important place is occupied by the search for optimal ozone treatment parameters. In laboratory conditions, data for mathematical modeling and optimization have been obtained. It has been established that the best treatment effect is achieved for the main wastewater pollutants, especially for synthetic surface-active substances (90 %) and BOD5 (65 %). Moreover, the processing time is 15 minutes at a dose of ozone of 20–25 mg/l. The concentration of total nitrogen decreases the least (20 %). It was found that a significant effect of disinfection by E. coli during ozonation is achieved by treating wastewater for 4 min at a dose of 3 mg/l of ozone. in a mixture of wastewater and natural water, this effect is achieved in 24 hours. The author's hypothesis of the gentle action of ozonation products for the widespread aquatic culture of Chlorella pyrenoidosa is confirmed. A significant effect of destruction under the influence of ozone of carcinogens was observed. It was found that up to 82 % of benzpyrene in wastewater is destroyed within 16 minutes at a dose of 15 mg/l. The authors conclude that ozonation is a universal method for the purification of biologically treated municipal wastewater, which has a significant effect on the destruction of many toxicants and pathogenic microflora, without the presence of ozonation products that inhibit the development of natural aquatic cultures. Further research should focus on finding low cost ozone synthesis methods

The process of regionalization of the state-territorial structure in modern european countries : constitutional and legal forms

Purpose: The paper attempts to identify the constitutional and legal forms of the regionalization process on the example of individual European countries. Design / Methodology / Approach: The authors put forward a hypothesis according to which each type of regionalization of the state-territorial structure ends with the adoption of the relevant normative legal act of constitutional significance. Findings: As a result of the study, the authors concluded that each type of regionalization process is accompanied by the adoption of an appropriate regulatory legal act. Given the fact that regionalization is inextricably linked with the state structure, it as an internal process of changing the constitutional and legal status of territorial units. Practical implications: Authors' development could be applied to improve the legal framework of Russia and European countries. Originality/Value: The contribution of the article is the authors' comprehensive approach in studying the regionalization concept, applying the historical, legislative and territorial background.peer-reviewe

Analysis of agriculture sustainable development in Russia

The article is focused on determining the current situation in Russia on the way to achieving the goals of sustainable development in agriculture. When analyzing the literature, it was revealed that most of the goals and objectives of sustainable development are already, to a greater or lesser degree, incorporated into the main strategic and program documents in Russia. Achievements in the implementation of SDG 2 “End hunger, achieve food security and improved nutrition and promote sustainable agriculture” were considered. Statistical indicators of agriculture sustainable development in Russia are analyzed. The principles for development of the agrifood systems sustainability concept are formulated and presented. In the last century and a half, the main task of Russia in country’s food security has been to feed its population. At the present day, it can be stated that this problem has been mainly solved

Aluminium Phthalocyanine Nanoparticles Application for Fluorescent Diagnostics and Photodynamic Therapy in Dentistry

Early diagnosis of tooth-enamel microcracks is of great importance in modern dentistry for caries prevention. It is known that the accumulation of the bacteria in the enamel microcracks can be reason of caries. A promising substance for early diagnostics of the accumulation of pathogenic microflora on the tooth enamel surface is aluminum phthalocyanine (AlPc). It could be observed that AlPc does not fluoresce in the nanoparticles form but in the monomeric molecular form it does. This allows identification of local pathological microflora accumulation within microcracks of the tooth enamel because AlPc nanoparticles (nAlPc) can be activated by pathological microflora. This paper describes the nAlPc application for fluorescent diagnostics (FD) of the enamel surface in vitro. To reduce the time from the beginning of interaction of nAlPc with the microflora to the appearance of nAlPc fluorescence, Protelan MST-35 surfactant was used as an additional activator. For FD in dentistry a model compound with nAlPc, Protelan MST-35 and with the complementary components was prepared. The following components were used as complementary: the methylparaben, carbopol, carboxymethyl cellulose, titanium dioxide, sodium phosphate, sodium saccharin and sorbitol, which are commonly used in toothpastes. Human teeth were used for the investigation of the interaction between nAlPc colloid and the model compound with nAlPc for the detection of microcracks and the areas of accumulation of pathogenic microflora on the enamel surface. Statistical processing of the experimental results showed the effectiveness of the surfactant usage for the additional activation of nAlPc and a reduction in the FD time. The application of nAlPc as a marker will make it possible to detect microcracks of the enamel tooth surface at the earliest stages and the areas of the pathogenic microflora accumulation, which can lead to the development of a caries. Keywords: fluorescent diagnostics, nanoparticles, aluminum phthalocyanine, Protelan MST-35, surfactants, enamel microcracks, tooth enamel

Perturbation theorems for Hele-Shaw flows and their applications

In this work, we give a perturbation theorem for strong polynomial solutions to the zero surface tension Hele-Shaw equation driven by injection or suction, so called the Polubarinova-Galin equation. This theorem enables us to explore properties of solutions with initial functions close to but are not polynomial. Applications of this theorem are given in the suction or injection case. In the former case, we show that if the initial domain is close to a disk, most of fluid will be sucked before the strong solution blows up. In the later case, we obtain precise large-time rescaling behaviors for large data to Hele-Shaw flows in terms of invariant Richardson complex moments. This rescaling behavior result generalizes a recent result regarding large-time rescaling behavior for small data in terms of moments. As a byproduct of a theorem in this paper, a short proof of existence and uniqueness of strong solutions to the Polubarinova-Galin equation is given.Comment: 25 page

Oxidative dehydrogenation of 2,3,5-trimethyl-1,4-hydroquinone in the presence of titanium dioxide hydrogel

Liquid-phase oxidative dehydrogenation of 2,3,5-trimethyl-1,4-hydroquinone in the presence of titanium dioxide hydrogel was studied by a kinetic method. Associative interactions between the substrate, oxidant, and gel were detected by voltammetry and ESR and IR spectroscopy.Russian Foundation for Basic Researc

Combination of fluorescent and spin labels: a powerful method for the optimization of hydrophilic membranes for the separation of oil-in-water emulsions

A new method for assessing the quality of fibre coating based on a combination of fluorescence microscopy and electron paramagnetic resonance is presented in this work. An influence of the carboxymethylcellulose/polyvinylamine gel preparation method on the mobility of the spin label was established. The mobility of the spin label changes from 3.5 ns in the case of a polyvinylamine solution to 12.8 ns in the case of a cross-linked gel on the surface of the glass fibre. A qualitative relationship was found between the mobility of the spin label in the gel applied to the glass fibre and the rate of spreading of crude oil over its surface. This method can be used to make membranes for the separation of water-in-oil emulsions

Endothelial dysfunction in patients after COVID-19

Relevance: Over the past two years, the COVID-19 infection has gained unprecedented relevance at the global level. All countries are spending significant resources in the fight against COVID-19 [1 2]. The purpose of the work is to clarify the issue of the state of the kidneys in the distant post-covid period (after 2-3 months), we conducted a study of the kidneys simultaneously with the desquamation of the endothelium, as one of the probable mechanisms of the nephrotic effect of the COVID-19 virus. Materials and methods: 29 people participated in the study. Patients with post-covid syndrome (n=10) were treated on an outpatient basis at the "KNP "Center of primary medical and sanitary (medical and sanitary) care No. 3, Odesa" of the Odesa City Council" in the period from 2020 to 2021. The control group consisted of patients without cardiovascular and endocrine disorders, comparable in terms of gender and age, who were examined in 2018 at the Odesa Regional Clinical Medical Center of the Odesa Regional Council. Endothelial dysfunction "ED" was determined by the intensity of desquamation of the endothelium according to the method of J. Hladovec as modified by M.M. Petryschev. and other. (2001).           Statistical analysis was performed using Status software [https://status-please.herokuapp.com/]. Mean values ​​(M) and its standard deviation (SD) were used to describe quantitative data, and percentages were used for qualitative data. For comparison, data were presented as M and standard error of the mean (SEM). Analysis of normality was performed using the Shapiro-Wilk test. Levene's test was used to assess homogeneity of variances. Differences between groups were determined using Student's t-test and one-way analysis of variance (ANOVA). The relationship and presence of correlation between the studied parameters was determined using the Pearson correlation coefficient (r). The correlation coefficient in the range of 0.5≤|r|<1 indicated a strong correlation relationship, 0.3≤|r|<0.5 – a relationship of medium strength, 0<|r|<0.3 – on a weak correlation. Statistical significance was established only at the level of p < 0.05. Results: 17 men and 12 women aged from 32 to 80 years who suffered from a disease of moderate severity participated in the study. The average age of the group of patients with post-covid syndrome (n=9) is 55.9±14.47 years, the average age of the control group (n=20) is 55.95±14.47 years. There were no complaints from the urinary system in the examined patients. According to blood serum tests, it was established that the creatinine level among patients with post-covid syndrome was on average 113±11.91 μmol/l, the urea level was 8.11±1.2 mmol/l, the GFR level was 55.06±12, 19 ml/min/1.73. m2. In the control group, the numbers were as follows: creatinine level was 73.2±8.5 μmol/l, mean urea level was 4.61 mmol/l, GFR was 94.78±13.76 ml/min/1.73 m2. Conclusions: 2-3 months after the transfer of COVID-19, the examined patients show signs of chronic kidney disease. After a disease with COVID-19, an increase in CEC is detected, which is a manifestation of endothelial dysfunction