Spatial analysis of tree species before forest fires

Spain is included in the top five European countries with the highest number of wildfires. The occurrence and magnitude of forest fires involves aspects of a very diverse nature, from those of a socio-economic, climatic, or physiographic nature, to those concerning fuel or the availability and quantity of resources and means of extinction. The distribution of wildfires in Galicia is not random and that fire occurrence may depend on ownership conflicts also a spatial dependence between productive or non-productive area exists. Satellite data play a major role in providing knowledge about fires by delivering rapid information to map fire-damaged areas precisely and promptly. In addition, the availability of large-scale data and the high temporal resolution offered by the Sentinel-2 satellite enables to classify and determine the land cover changes with high accuracy. This study describes a methodology to detect burned areas and analyse the Land Cover and Land Use (LCLU) classes present in these areas during the period of 5 years (2016–2021) by Sentinel-2 images. The training areas were obtained by photointerpretation and the image classification was performed using the Random Forest algorithm which shows an overall accuracy range between 80–85%. The methodology concluded that Lobios and Muiños were the most affected municipalities by wildfires. Additionally, the spatial analysis determined that the Deciduous Forest mainly composed by Quercus sp. were the most affected in 2017 followed by Coniferous Forest mainly composed by Pinus sp.in 2016. Although, Scrub and Rock are the classes more affected for wildfire during 2016–2020 period.Universidade de Vigo | Ref. 00VI 131H 6410211Agencia Estatal de Investigación | Ref. PCI2020-120705-2Xunta de Galicia | Ref. ED481B-2019-061Xunta de Galicia | Ref. ED431C 2020/0

Proteomic analysis of Cucumis Melo L. Phloem as an approach to study resistance to Bemisia Tabaci gen

Comunicaciones a congreso

UAV and satellite imagery applied to alien species mapping in NW Spain

Image classification stands as an essential tool for automated mapping, that is demanded by agencies and stakeholders dealing with geospatial information. Decreasing costs or UAV-based surveying and open access to high resolution satellite images such as that provided by European Union’s Copernicus programme are the basis for multi-temporal landscape analysis and monitoring. Besides that, invasive alien species are considered a risk for biodiversity and their inventory is needed for further control and eradication. In this work, a methodology for semi-automatic detection of invasive alien species through UAV surveying and Sentinel 2 satellite monitoring is presented and particularized for Acacia dealbata Link species in the province of Pontevedra, in NW Spain. We selected a scenario with notable invasion of Acaciae and performed a UAS surveying to outline feasible training areas. Such areas were used as bounds for obtaining a spectral response of the cover from Sentinel 2 images with a level of processing 2A, that was used for invasive area detection. Sparse detected areas were treated as a seed for a region growing step to obtain the final map of alien species.Deputación de Pontevedra | Ref. 17/410.1720.789.0

Modelling and evaluation of land use changes through satellite images in a multifunctional catchment: social, economic and environmental implications

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGFloods are recurrent phenomena with significant environmental and socio-economic impacts. The risk of flooding increases when land use changes. The objective of this research is to detect land cover changes via Sentinel-2 images in the Umia Basin (Galicia, NW Spain) in 2016–2021 and to analyse the associated flood risk. This study focuses on how forest use and nature-based solutions (NBS) can reduce the risk and hazard of flooding in cities and crops in the high-risk area. A flood simulation was performed with the land use obtained from Sentinel-2 (Observed) and three more simulations were performed changing the location of afforestation and NBS, i.e. “S-Upstream”, “S-Downstream” and “S-Total”. Finally, the environmental, economic and social impacts of the scenarios designed and estimated are analysed and discussed. Land cover change was successfully monitored with Sentinel-2 imagery. The catchment area showed noteworthy changes in land use, most notably for the category of trees, which covered 6700 ha in 2016 and 10,911 ha in 2021. However riparian vegetation decreased by almost 11%. For the flood hazard simulations, an average reduction in peak discharge was obtained for all three scenarios (9.3% for S-Up; 8.6% for S-Down and 13% for S-Total). From the economic perspective, all three scenarios show a positive net present value for the period studied. However, S-Down is the scenario with the lowest benefits (€15,476,487), while S-Up and S-Total show better values at €29,580,643 and €65,158,130 respectively. However, investment cost is much higher for the S-Total scenario, and upstream actions affect the whole catchment, so S-Up is the best decision. This study concludes that the information provided by satellites is a large-scale analysis tool for small heterogeneous plots that facilitates the comprehensive analysis of a territory. This information can be incorporated into flood analysis models, facilitating simulation through the use of NBS. It has been proven that the use of reforestation upstream only is almost as beneficial as reforestation in the entire catchment and is economically more viable. This confirms that the methodology used reduces flood hazard, despite the territorial complexity, facilitating decision making on the use of NBS.Universidade de Vig

Assessment of high spatial resolution satellite imagery for monitoring riparian vegetation: riverine management in the smallholding

Riverine habitats are essential ecotones that bridge aquatic and terrestrial ecosystems, providing multiple ecosystem services. This study analyses the potential use of high-resolution satellite imagery, provided by the WorldView-2 satellite, in order to assess its viability for monitoring riparian ecosystems. It is performed by calculating the riparian strip quality index (RSQI) and calibrating it with the riparian quality index (QBR). The methodology was implemented in the Umia River, which is characterised by elevated anthropogenic pressures (located in the northwest of Spain). The results obtained by the method have a 92% of veracity and a kappa coefficient of 0.88. The average quality value obtained for the RSQI index was 71.57, while the average value for the QBR was 55.88. This difference could be attributed to the fact that the former does not differ between autochthonous and non-autochthonous vegetation. The areas with more accurate mapping corresponded to stretches of vegetation with optimal cover (80–50%), with good connectivity with the adjacent forest ecosystem and few or no presence of invasive plants. The worst-scoring sites had the next characteristics: low connectivity (< 10%), low forest cover (< 10%) and a higher presence of invasive plants. The degradation of vegetation could be explained by the presence of agriculture and deficient land use rationing caused by the type of ownership of the study area. The application of this index through satellite images will facilitate the environmental governance of multiple ecosystems and in special riparian ecosystems, obtaining a quick and objective methodology, easily replicable in other basins.Universidade de Vigo/CISUGXunta de Galicia | Ref. ED431B 2022/1

Operational study of drone spraying application of phytosanitary products in vineyards

The use of drones in topics related to precision agriculture to improve the efficiency in the application of phytosanitary products to vineyards increases every day. Drones are especially productive in difficult orographic terrains, where other mechanical systems such as tractors cannot be used. This study shows the development and implementation of a methodology to determine key parameters to decide the suitability of a drone to a spraying task (i.e. spraying time for a certain parcel, number or tank refills required), taking into account the technical specifications of a certain commercial model. For the validation, the data of a vineyard belonging to the Rías Baixas appellation of origin (NW Spain) and the technical specifications of drones from three different manufacturers (i.e. DJI, Hylio and Yamaha) are used. Results show that the Hylio AD122 with a phytosanitary tank of 22 L provides the best performance, with a productivity around 6 minutes per hectare.La utilización de drones en tareas relacionadas con la agricultura de precisión para mejorar la eficiencia en la aplicación de productos fitosanitarios en viñedos es cada vez mayor. Los drones son especialmente eficientes en terrenos con orografía difícil, donde no se pueden emplear otros sistemas mecánicos como tractores. Este estudio muestra el desarrollo e implementación de una metodología para determinar parámetros clave que decidan la adecuación de un drone determinado a una tarea de fumigación (por ejemplo, el tiempo de fumigación para una cierta parcela o el número de tanques requeridos para dicha fumigación), teniendo en cuenta las especificaciones técnicas de un determinado modelo comercial. Para la validación de la metodología, se han utilizado los datos de un viñedo que pertenece a la denominación de origen Rías Baixas (Noroeste de Espala) y las características técnicas de tres fabricantes diferentes de drones (DJI, Hylio y Yamaha). Los resultados obtenidos muestran como el Hylio AD122 con un tanque de fitosanitario de 22 L provee el mejor rendimiento, con una productividad de aproximadamente 6 minutos por hectáre

A Multi-Omics Analysis Pipeline for the Metabolic Pathway Reconstruction in the Orphan Species Quercus ilex

Holm oak (Quercus ilex) is the most important and representative species of the Mediterranean forest and of the Spanish agrosilvo-pastoral “dehesa” ecosystem. Despite its environmental and economic interest, Holm oak is an orphan species whose biology is very little known, especially at the molecular level. In order to increase the knowledge on the chemical composition and metabolism of this tree species, the employment of a holistic and multi-omics approach, in the Systems Biology direction would be necessary. However, for orphan and recalcitrant plant species, specific analytical and bioinformatics tools have to be developed in order to obtain adequate quality and data-density before to coping with the study of its biology. By using a plant sample consisting of a pool generated by mixing equal amounts of homogenized tissue from acorn embryo, leaves, and roots, protocols for transcriptome (NGS-Illumina), proteome (shotgun LC-MS/MS), and metabolome (GC-MS) studies have been optimized. These analyses resulted in the identification of around 62629 transcripts, 2380 protein species, and 62 metabolites. Data are compared with those reported for model plant species, whose genome has been sequenced and is well annotated, including Arabidopsis, japonica rice, poplar, and eucalyptus. RNA and protein sequencing favored each other, increasing the number and confidence of the proteins identified and correcting erroneous RNA sequences. The integration of the large amount of data reported using bioinformatics tools allows the Holm oak metabolic network to be partially reconstructed: from the 127 metabolic pathways reported in KEGG pathway database, 123 metabolic pathways can be visualized when using the described methodology. They included: carbohydrate and energy metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, and biosynthesis of secondary metabolites. The TCA cycle was the pathway most represented with 5 out of 10 metabolites, 6 out of 8 protein enzymes, and 8 out of 8 enzyme transcripts. On the other hand, gaps, missed pathways, included metabolism of terpenoids and polyketides and lipid metabolism. The multi-omics resource generated in this work will set the basis for ongoing and future studies, bringing the Holm oak closer to model species, to obtain a better understanding of the molecular mechanisms underlying phenotypes of interest (productive, tolerant to environmental cues, nutraceutical value) and to select elite genotypes to be used in restoration and reforestation programs, especially in a future climate change scenario

Multiomics Molecular Research into the Recalcitrant and Orphan Quercus ilex Tree Species: Why, What for, and How

The holm oak (Quercus ilex L.) is the dominant tree species of the Mediterranean forest and the Spanish agrosilvopastoral ecosystem, “dehesa.” It has been, since the prehistoric period, an important part of the Iberian population from a social, cultural, and religious point of view, providing an ample variety of goods and services, and forming the basis of the economy in rural areas. Currently, there is renewed interest in its use for dietary diversification and sustainable food production. It is part of cultural richness, both economically (tangible) and environmentally (intangible), and must be preserved for future generations. However, a worrisome degradation of the species and associated ecosystems is occurring, observed in an increase in tree decline and mortality, which requires urgent action. Breeding programs based on the selection of elite genotypes by molecular markers is the only plausible biotechnological approach. To this end, the authors’ group started, in 2004, a research line aimed at characterizing the molecular biology of Q. ilex. It has been a challenging task due to its biological characteristics (long life cycle, allogamous, high phenotypic variability) and recalcitrant nature. The biology of this species has been characterized following the central dogma of molecular biology using the omics cascade. Molecular responses to biotic and abiotic stresses, as well as seed maturation and germination, are the two main objectives of our research. The contributions of the group to the knowledge of the species at the level of DNA-based markers, genomics, epigenomics, transcriptomics, proteomics, and metabolomics are discussed here. Moreover, data are compared with those reported for Quercus spp. All omics data generated, and the genome of Q. ilex available, will be integrated with morphological and physiological data in the systems biology direction. Thus, we will propose possible molecular markers related to resilient and productive genotypes to be used in reforestation programs. In addition, possible markers related to the nutritional value of acorn and derivate products, as well as bioactive compounds (peptides and phenolics) and allergens, will be suggested. Subsequently, the selected molecular markers will be validated by both genome-wide association and functional genomic analyses

High incidence of PTSD diagnosis and trauma-related symptoms in a trauma exposed bipolar I and II sample

Post-traumatic stress disorder (PTSD) is an established comorbidity in Bipolar Disorder (BD), but little is known about the characteristics of psychological trauma beyond a PTSD diagnosis and differences in trauma symptoms between BD-I and BD-II. (1) To present characteristics of a trauma-exposed BD sample; (2) to investigate prevalence and trauma symptom profile across BD-I and BD-II; (3) to assess the impact of a lifetime PTSD diagnosis vs. a history of trauma on BD course; and (4) to research the impacts of sexual and physical abuse. This multi-center study comprised 79 adult participants with BD with a history of psychological trauma and reports baseline data from a trial registered in Clinical Trials (; ref: NCT02634372). Clinical variables were gathered through clinical interview, validated scales and a review of case notes. The majority (80.8%) of our sample had experienced a relevant stressful life event prior to onset of BD, over half of our sample 51.9% had a lifetime diagnosis of PTSD according to the Clinician Administered PTSD scale. The mean Impact of Event Scale-Revised scores indicated high levels of trauma-related distress across the sample, including clinical symptoms in the PTSD group and subsyndromal symptoms in the non-PTSD group. Levels of dissociation were not higher than normative values for BD. A PTSD diagnosis (vs. a history of trauma) was associated with psychotic symptoms [2(1) = 5.404, p = 0.02] but not with other indicators of BD clinical severity. There was no significant difference between BD-I and BD-II in terms of lifetime PTSD diagnosis or trauma symptom profile. Sexual abuse significantly predicted rapid cycling [2(1) = 4.15, p = 0.042], while physical abuse was not significantly associated with any clinical indicator of severity. Trauma load in BD is marked with a lack of difference in trauma profile between BD-I and BD-II. Although PTSD and sexual abuse may have a negative impact on BD course, in many indicators of BD severity there is no significant difference between PTSD and subsyndromal trauma symptoms. Our results support further research to clarify the role of subsyndromic PTSD symptoms, and highlight the importance of screening for trauma in BD patients

Treatment variability and its relationships to outcomes among patients with Wernicke's encephalopathy: A multicenter retrospective study

Background: Despite guidelines and recommendations, Wernicke's encephalopathy (WE) treatment lacks evidence, leading to clinical practice variability.Aims: Given the overall lack of information on thiamine use for WE treatment, we analyzed data from a large, well-characterized multicenter sample of patients with WE, examining thiamine dosages; factors associated with the use of different doses, frequencies, and routes; and the influence of differences in thiamine treatment on the outcome.Methods: This retrospective study was conducted with data from 443 patients from 21 centers obtained from a nationwide registry of the Spanish Society of Internal Medicine (from 2000 to 2012). Discharge codes and Caine criteria were applied for WE diagnosis, and treatment-related (thiamine dosage, frequency, and route of administration) demographic, clinical, and outcome variables were analyzed.Results: We found marked variability in WE treatment and a low rate of high-dose intravenous thiamine administration. Seventy-eight patients out of 373 (20.9%) received > 300 mg/day of thiamine as initial dose. Patients fulfilling the Caine criteria or presenting with the classic WE triad more frequently received parenteral treatment. Delayed diagnosis (after 24 h hospitalization), the fulfillment of more than two Caine criteria at diagnosis, mental status alterations, and folic acid deficiency were associated significantly with the lack of complete recovery. Malnutrition, reduced consciousness, folic acid deficiency, and the lack of timely thiamine treatment were risk factors for mortality.Conclusions: Our results clearly show extreme variability in thiamine dosages and routes used in the management of WE. Measures should be implemented to ensure adherence to current guidelines and to correct potential nutritional deficits in patients with alcohol use disorders or other risk factors for WE