359 research outputs found
Les canaux calciques dépendants du voltage au coeur de la douleur
Les canaux calciques dĂ©pendants du voltage reprĂ©sentent une des voies principales dâentrĂ©e du calcium dans la cellule nerveuse oĂč ils participent activement Ă lâexcitabilitĂ© cellulaire et aux processus molĂ©culaires de la transmission synaptique. Ils ont, de ce fait, Ă©tĂ© depuis longtemps la cible pharmacologique dâanalgĂ©siques et ce, avant mĂȘme que leur implication dans la physiologie de la nociception ait rĂ©ellement Ă©tĂ© dĂ©montrĂ©e. Ces derniĂšres annĂ©es, la caractĂ©risation molĂ©culaire de plus en plus fine de ces canaux et de leurs sous-unitĂ©s rĂ©gulatrices, ainsi que la dĂ©monstration de leur implication dans les processus nociceptifs, a permis de dĂ©finitivement considĂ©rer ces structures comme des cibles pharmacologiques de premier choix pour le traitement de la douleur. La recherche dâinhibiteurs spĂ©cifiques des canaux calciques dĂ©pendants du voltage laisse ainsi entrevoir le dĂ©veloppement de nouvelles molĂ©cules analgĂ©siques fortement prometteuses.Voltage-dependent calcium channels represent a major pathway of calcium entry into neurons, where they participate actively to cell excitability and to the molecular processes of synaptic transmission. For that reason, they have been the direct or indirect pharmacological targets of analgesics and this long before their implication in the physiology of nociception had been demonstrated. These last years, the still more refined molecular characterization of these channels and their associated regulatory subunits and the demonstration of their implication in nociceptive processes indicates that these structures are prime pharmacological targets for the management of pain. Herein, we detail the recent breakthroughs on calcium channel structure, function and pharmacology, review the implication of calcium channels in the transmission of nociception, and evaluate their importance as targets for the treatment of pain perception. The search for specific inhibitors of voltage-dependent calcium channels appears as a prelude to the development of new promising analgesic molecules
[Role of P/Q calcium channel in familial hemiplegic migraine]
International audienceVoltage-dependent calcium channels constitute one of the main pathways of calcium entry into neurons. They are the principal actors of synaptic transmission by controlling the release of neurotransmitters. They also contribute to numerous other cell functions, such as gene expression or synaptogenesis. These channels, by their essential cell functions, are at the origin of numerous channelopathies resulting from mutations of the genes encoding their different subunits. Familial Hemiplegic Migraine (FHM) represents one such example of these channelopathies. In this human disease, genetic studies have demonstrated the implication of the CACNA1A gene in a type 1 form of FHM. This gene encodes for the Ca(v)2.1 subunit of P/Q calcium channels and is the target of numerous mutations affecting the properties of channel activity. The question on how discrete mutations of this gene are able to alter the activity of the channel and contribute to the physiopathology of FHM remains an open question. The functional characterization of mutated channels in various heterologous expression systems, as well as in vivo in an animal model, provides a molecular scheme of the physiopathology of FHM in which neurons, astrocytes and blood circulation act in concert
How do G proteins directly control neuronal Ca2+ channel function?
Ca2+ entry into neuronal cells is modulated by the activation of numerous G-protein-coupled receptors (GPCRs). Much effort has been invested in studying direct G-protein-mediated inhibition of voltage-dependent CaV2 Ca2+ channels. This inhibition occurs through a series of convergent modifications in the biophysical properties of the channels. An integrated view of the structural organization of the Gbetagamma-dimer binding-site pocket within the channel is emerging. In this review, we discuss how variable geometry of the Gbetagamma binding pocket can yield distinct sets of channel inhibition. In addition, we propose specific mechanisms for the regulation of the channel by G proteins that take into account the regulatory input of each Gbetagamma binding element
Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of âBrevibacterium helvolumâ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.
A Gram-positive, molinate-degrading bacterium, strain ON4T (=DSM 13485T=LMG 21909T),
was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was
strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of
10â41 6C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on
D-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent
in the family Microbacteriaceae, showing the highest 16S rDNA similarity (~95 %) to members
of the genus Curtobacterium and âBrevibacterium helvolumâ DSM 20419 (=ATCC 13715).
The latter was reported to have the cell-wall peptidoglycan type B2c and the major menaquinone
MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the
phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously
described genera and their distinctive phenotypic characteristics, it is proposed that strains
ON4T and âB. helvolumâ DSM 20419 be classified as two novel genera and species, with the
respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus
gen. nov., sp. nov
Importance of voltage-dependent inactivation in N-type calcium channel regulation by G-proteins.: Channel inactivation in G-protein regulation
International audienceDirect regulation of N-type calcium channels by G-proteins is essential to control neuronal excitability and neurotransmitter release. Binding of the G(betagamma) dimer directly onto the channel is characterized by a marked current inhibition ("ON" effect), whereas the pore opening- and time-dependent dissociation of this complex from the channel produce a characteristic set of biophysical modifications ("OFF" effects). Although G-protein dissociation is linked to channel opening, the contribution of channel inactivation to G-protein regulation has been poorly studied. Here, the role of channel inactivation was assessed by examining time-dependent G-protein de-inhibition of Ca(v)2.2 channels in the presence of various inactivation-altering beta subunit constructs. G-protein activation was produced via mu-opioid receptor activation using the DAMGO agonist. Whereas the "ON" effect of G-protein regulation is independent of the type of beta subunit, the "OFF" effects were critically affected by channel inactivation. Channel inactivation acts as a synergistic factor to channel activation for the speed of G-protein dissociation. However, fast inactivating channels also reduce the temporal window of opportunity for G-protein dissociation, resulting in a reduced extent of current recovery, whereas slow inactivating channels undergo a far more complete recovery from inhibition. Taken together, these results provide novel insights on the role of channel inactivation in N-type channel regulation by G-proteins and contribute to the understanding of the physiological consequence of channel inactivation in the modulation of synaptic activity by G-protein coupled receptors
Intracellular Ca2+ Homeostasis In Rat Fast-Twitch Skeletal Muscle Fibers During Disuse atrophy
Aureobacterium resistens sp. nov., exhibiting vancomycin resistance and teicoplanin susceptibility
Two similar strains of a coryneform bacterium were isolated from human clinical material. Both strains were resistant to vancomycin but susceptible to teicoplanin. Detailed biochemical, chemotaxonomical, and molecular genetic investigations revealed that both isolates were members of a hitherto undescribed species of the genus Aureobacterium. The name Aureobacterium resistens sp. nov. is proposed for the new bacterium and the type strain is CCUG 3831
Eine monumentale Dankesgabe: Trajans Neubau der Heiligen StraĂe von Milet nach Didyma
Durch Bau- bzw. Dedikationsinschriften in lateinischer und griechischer Sprache ist seit langem bekannt, dass Trajan die Heilige StraĂe von Milet zum Heiligtum des Apollon von Didyma neu anlegen lieĂ. Eine weitere, hier publizierte griechische Inschrift mit neuen Einzelheiten gibt Anlass, die gesamte vorhandene epigraphische PraÌsentation in revidierter Form vorzulegen und die Inszenierung des Bauprogramms mit seinem politischen Hintergrund eingehend zu analysieren. Es dokumentiert â so das Ergebnis â dauerhaft Trajans cura, liberalitas und vor allem pietas in Form einer monumentalen Dankesgabe an die Stadt und ihren Orakelgott, der die Herrschaft des Kaisers vorausgesagt hatte.Through building and dedicatory inscriptions written in Latin and Greek, it has long been known that Trajan had the sacred road from Miletus to the sanctuary of Apollo at Didyma reconstructed. Another Greek inscription, to be published in this article, provides new details and offers the occasion to present the entire epigraphical documentation in a revised form, analysing in-depth the political staging of this building programme. Hence, it records Trajanâs cura, liberalitas, and above all his pietas in a permanent way by offering a monumental gift to the city and her oracle god who had foretold the emperorâs rule.On sait depuis longtemps, graÌce au teÌmoignage de deÌdicaces eÌpigraphiques en latin et en grec, que Trajan fit reÌnover la route sacreÌe qui meÌne de Milet jusquâau sanctuaire dâApollon de Didymes. Une des inscriptions grecques, publieÌe ici avec de nouveaux deÌtails, donne lâoccasion de reprendre, en le preÌsentant sous une forme reÌviseÌe, lâensemble du dossier eÌpigraphique disponible et dâanalyser de manieÌre deÌtailleÌe la mise en sceÌne du programme de construction dans son contexte politique. Cette documentation met en relief â comme le reÌveÌle le reÌsultat â sur le long terme la cura de Trajan, sa liberalitas et avant tout sa pietas qui se manifestent sous la forme dâun don de reconnaissance monumental envers la ville et envers sa diviniteÌ oraculaire qui avait preÌdit le reÌgne de lâempereur
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