BcAtf1, a global regulator, controls various differentiation processes and phytotoxin production in Botrytis cinerea

Atf1-homologous basic region leucine zipper (bZIP) transcription factors are known to act downstream of the stress-activated mitogen-activated protein kinase (SAPK) cascade in mammals, as well as in several fungi; they regulate the transcription of genes involved in the general stress response. Functional analyses of BcAtf1 in Botrytis cinerea show that it is also connected to the SAPK BcSak1, as it shares several stress response target genes. However, Dbcatf1 mutants are not hypersensitive to osmotic or oxidative stress, as are Dbcsak1 mutants. Both BcSak1 and BcAtf1 are regulators of differentiation, but their roles in these processes are almost inverse as, in contrast with Dbcsak1, Dbcatf1 mutants are significantly impaired in conidia production and do not differentiate any sclerotia. They show extremely vigorous growth in axenic culture, with a thick layer of aerial hyphae and a marked increase in colonization efficiency on different host plants and tissues. In addition, the sensitivity to cell wall-interfering agents is increased strongly. Microarray analyses demonstrate that the loss of BcAtf1 leads to extensive transcriptional changes: apart from stress response genes, the expression of a broad set of genes, probably involved in primary metabolism, cell wall synthesis and development, is affected by BcAtf1. Unexpectedly, BcAtf1 also controls secondary metabolism: the mutant contains significantly elevated levels of phytotoxins. These data indicate that BcAtf1 controls a diversity of cellular processes and has broad regulatory functions

Understanding the sugar beet holobiont for sustainable agriculture

The importance of crop-associated microbiomes for the health and field performance of plants has been demonstrated in the last decades. Sugar beet is the most important source of sucrose in temperate climates, and—as a root crop—yield heavily depends on genetics as well as on the soil and rhizosphere microbiomes. Bacteria, fungi, and archaea are found in all organs and life stages of the plant, and research on sugar beet microbiomes contributed to our understanding of the plant microbiome in general, especially of microbiome-based control strategies against phytopathogens. Attempts to make sugar beet cultivation more sustainable are increasing, raising the interest in biocontrol of plant pathogens and pests, biofertilization and –stimulation as well as microbiome-assisted breeding. This review first summarizes already achieved results on sugar beet-associated microbiomes and their unique traits, correlating to their physical, chemical, and biological peculiarities. Temporal and spatial microbiome dynamics during sugar beet ontogenesis are discussed, emphasizing the rhizosphere formation and highlighting knowledge gaps. Secondly, potential or already tested biocontrol agents and application strategies are discussed, providing an overview of how microbiome-based sugar beet farming could be performed in the future. Thus, this review is intended as a reference and baseline for further sugar beet-microbiome research, aiming to promote investigations in rhizosphere modulation-based biocontrol options

Identification of plant genotype dependent microbiome recruitment associated with disease resistance against root rot in peas

The cultivation of pea (Pisum sativum) is highly constrained by various soil-borne pathogens. Together these pathogens form a pea root rot complex (PRRC) and trigger soil fatigue. Microbiome-mediated disease resistance poses a possible mechanism to mitigate yield loss through PRRC. It is however largely unknown how the PRRC interacts with other members of the root microbiome and how this affects plant resistance. Here, we compared the root microbiome of 252 pea lines in a controlled soil-based phenotyping assay that was previously shown to predict field-relevant resistance against PRRC. Root bacteria and fungi were characterized by 16S rRNA and ITS amplicon sequencing. We analyzed alpha diversity and microbial community composition, and identified heritable hub OTUs. Based on differential abundance analysis we further identified heritable bacterial and fungal hub taxa that are associated with root rot resistance. Subsequent genome-wide association studies revealed plant genomic regions that are significantly correlated with beneficial hub taxa and overall microbial community composition. In a next step, the identified genetic markers will be used to select pea breeding material for field validation of microbiome-mediated resistance against PRRC. This work demonstrates the potential of microbiome-assisted breeding to promote sustainable farming practices