Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology.

An amendment to this paper has been published and can be accessed via a link at the top of the paper

Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways

Genetic tool development in marine protists: emerging model organisms for experimental cell biology

Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways

Genetic transformation of the dinoflagellate chloroplast.

Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation technology. By making use of the plasmid-like fragmented chloroplast genome, we have introduced novel genetic material into the dinoflagellate chloroplast genome. We have shown that the introduced genes are expressed and confer the expected phenotypes. Genetically modified cultures have been grown for 1 year with subculturing, maintaining the introduced genes and phenotypes. This indicates that cells continue to divide after transformation and that the transformation is stable. This is the first report of stable chloroplast transformation in dinoflagellate algae

SEIS first year: nm/s^2 (and less) broadband seismology on Mars and first steps in Mars-Earth-Moon comparative seismology. (Invited)

AGU Fall Meeting 2019 in San Francisco , 9-13 December 2019EIS/InSIght teamInSight is the first planetary mission with a seismometer package, SEIS, since the Apollo Lunar Surface Experiments Package. SEIS is complimented by APSS, which has as a goal to document the atmospheric source of seismic noise and signals. Since June 2019, SEIS has been delivering 6 axis 20 sps continuous seismic data, a rate one order of magnitude larger originally planned. More than 50 events have been detected by the end of July 2019 but only three have amplitudes significantly above the SEIS instrument requirement. Two have clear and coherent arrivals of P and S waves, enabling location, diffusion/attenuation characterization and receiver function analysis. The event¿s magnitudes are likely ¿ 3 and no clear surface waves nor deep interior phases have been identified. This suggests deep events with scattering along their final propagation paths and with large propagation differences as compared to Earth and Moon quakes. Most of the event¿s detections are made possible due to the very low noise achieved by the instrument installation strategy and the very low VBB self-noise. Most of the SEIS signals have amplitudes of spectral densities in the 0.03-5Hz frequency bandwidth ranging from 10-10 m/s2/Hz1/2 to 5 10-9 m/s2/Hz1/2. The smallest noise levels occurs during the early night, with angstrom displacements or nano-radian tilts. This monitors the elastic and seismic interaction of a planetary surface with its atmosphere, illustrated not only by a wide range of SEIS signals correlated with pressure vortexes, dust devils or wind activity but also by modulation of resonances above 1 Hz, amplified by ultra-low velocity surface layers. After about one half of a Martian year, clear seasonal changes appear also in the noise, which will be discussed. One year after landing, the seismic noise is therefore better and better understood, and noise correction techniques begun to be implemented, either thanks to the APSS wind and pressure sensors, or by SEIS only data processing techniques. These data processing techniques open not only the possibility of better signal to noise ratio of the events, but are also used for various noise auto-correlation techniques as well as searches of long period signals. Noise and seismic signals on Mars are therefore completely different from what seismology encountered previously on Earth and Moon

Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology.

An amendment to this paper has been published and can be accessed via a link at the top of the paper

Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology.

An amendment to this paper has been published and can be accessed via a link at the top of the paper