176 research outputs found

    Exploring Cosmology and Structure Formation via High-z Galaxies

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    This thesis exploits the large-scale structure of the Universe via observations over a wide redshift range, with the aim of constraining the current cosmological models and galaxy formation physics. We present the eXtreme Multiplex Spectrograph (XMS), a proposed spectrograph that can map simultaneously 4000 Emission Line Galaxies (ELGs) and Luminous Red Galaxies (LRGs) in the range 0.4 ≤ z ≤ 0.8, with a success rate of 88%. Figures of merit clearly indicate that XMS is better or even competitive compared to future surveys for measurements of the gravitational growth rate, Baryon Acoustic Oscillations (BAO) and dark-matter halo mass function. Next, by selecting a unique photometric sample of 130, 000 LRGs in the Sloan Digital Sky Survey (SDSS) Stripe 82, with an estimated average redshift z ∼ 1, we perform a clustering analysis and compare the clustering evolution of the high-z Stripe 82 LRGs to lower-z LRGs. An immediate feature of the Stripe 82 LRGs clustering is a power excess at large scales. This behaviour is not expected within the ΛCDM model, making the conclusion of a slow clustering evolution as observed for the lower-z LRGs, non-trivial. Only Non-Gaussian models are able to describe the large scale clustering of the Stripe 82 LRGs. From follow up spectroscopic observations of a subsample of the Stripe 82 LRGs, we confirm that the average red- shift of our sample is z ∼ 0.9, while the slow clustering evolution of the LRGs is now slightly more favoured. However, Non-Gaussianity is still detected at a 2σ level. Finally, from the largest and deepest near-infrared field to present, the UltraVISTA survey, we select ≈ 4000 Distant Red Galaxies (DRGs) and study the largest galaxy separations ever probed with these massive galaxies. In agreement with previous results, UltraVISTA DRGs are strongly clustered objects. Furthermore, they show stronger clustering within their brighter K-limited samples, that could possibly imply luminosity segregation. Their connection to the local descendants cannot yet be established

    CHART FOR THE PRINCIPLE OF THE WORLD

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    CHART FOR THE PRINCIPLE OF THE WORL

    Introducing Green, Eco-Friendly Practices and Circular Economy Principles in Vocational Education Through a Novel Analysis-Synthesis Method: Design, Implementation and Evaluation

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    Purpose: A novel, Project- and Collaborative Learning-based educational method is proposed, implemented and evaluated in this paper. The aim is to exploit hands-on laboratory modules in Vocational Education in order to experientially introduce students to green, eco-friendly practices and the principles of sustainability and circular economy. Besides their apparent individual and social benefits, such knowledge and skills are also expected to raise qualifications and employability of Vocational Education graduates. Methods: The proposed method is tested through a quasi-experimental methodology, via an educational intervention with a class of Vocational Lyceum students, in the field of Electrical and Electronics Engineering. The learning content focuses on the reclaiming and reuse of operational components from damaged electrical/electronic equipment at end-of-life stage. Through repeated Analysis and Synthesis phases, students learn to extract, measure, classify and reuse operational components either to repair similar equipment or to design and construct novel devices. Results: Evaluation is carried out via closed and open type activities as well as by observation sheets of the teacher. Learning outcomes are evaluated through knowledge post-tests of the closed type while social/emotional outcomes are evaluated through questionnaires. Evaluation results indicate that the proposed method does produce cognitive and social/emotional skills gains for the students. The development of metacognitive skills and the stimulation of imagination and innovative thinking in the students is also observed by the teacher, but not formally evaluated due to practical constraints. Conclusions: The proposed method is implemented and pilot-tested with positive results both as to the cognitive and as to the social/emotional domain - yet, these results are of an indicative value, due to the limited scale of the educational intervention. Future research is necessary in order to evaluate the proposed method in extent and possibly compare results across education grades or engineering fields, as the method is generic enough to be easily adaptable for different ages/grades and engineering/technical fields of study

    45S rDNA external transcribed spacer organization reveals new phylogenetic relationships in Avena genus

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    Research ArticleThe genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid (AABB or AACC) and hexaploid species (AACCDD), constituting an interesting model for phylogenetic analysis. The aim of this work was to characterize 45S rDNA intergenic spacer (IGS) variability in distinct species representative of Avena genome diversity±A. strigosa (AA), A. ventricosa (CvCv), A. eriantha (CpCp), A. barbata (AABB), A. murphyi (AACC), A. sativa (AACCDD) and A. sterilis (AACCDD) through the assessment of the 5' external transcribed spacer (5'-ETS), a promising IGS region for phylogenetic studies poorly studied in Avena genus. In this work, IGS length polymorphisms were detected mainly due to distinct 5'-ETS sequence types resulting from major differences in the number and organization of repeated motifs. Although species with A genome revealed a 5'-ETS organization (A-organization) similar to the one previously described in A. sativa, a distinct organization was unraveled in C genome diploid species (C-organization). Interestingly, such new organization presents a higher similarity with other Poaceae species than A-genome sequences, supporting the hypothesis of C-genome being the ancestral Avena genome. Additionally, polyploid species with both genomes mainly retain the A-genome 5'-ETS organization, confirming the preferential elimination of C-genome sequences in Avena polyploid species. Moreover, 5'-ETS sequences phylogenetic analysis consistently clustered the species studied according to ploidy and genomic constitution supporting the use of ribosomal genes to highlight Avena species evolutive pathways.info:eu-repo/semantics/publishedVersio

    The Clustering of Galaxies in SDSS-III DR9 Baryon Oscillation Spectroscopic Survey: Constraints on Primordial Non-Gaussianity

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    We analyze the density field of 264,283 galaxies observed by the Sloan Digital Sky Survey (SDSS)-III Baryon Oscillation Spectroscopic Survey (BOSS) and included in the SDSS data release nine (DR9). In total, the SDSS DR9 BOSS data includes spectroscopic redshifts for over 400,000 galaxies spread over a footprint of more than 3,000 deg^2. We measure the power spectrum of these galaxies with redshifts 0.43 < z < 0.7 in order to constrain the amount of local non-Gaussianity, f_NL,local, in the primordial density field, paying particular attention to the impact of systematic uncertainties. The BOSS galaxy density field is systematically affected by the local stellar density and this influences the ability to accurately measure f_NL,local. In the absence of any correction, we find (erroneously) that the probability that f_NL,local is greater than zero, P(f_NL,local >0), is 99.5%. After quantifying and correcting for the systematic bias and including the added uncertainty, we find -45 < f_NL,local 0) = 91.0%. A more conservative approach assumes that we have only learned the k-dependence of the systematic bias and allows any amplitude for the systematic correction; we find that the systematic effect is not fully degenerate with that of f_NL,local, and we determine that -82 < f_NL,local < 178 (at 95% confidence) and P(f_NL,local >0) = 68%. This analysis demonstrates the importance of accounting for the impact of Galactic foregrounds on f_NL,local measurements. We outline the methods that account for these systematic biases and uncertainties. We expect our methods to yield robust constraints on f_NL,local for both our own and future large-scale-structure investigations.Comment: Matches version to be published in MNRAS. While in press, we found an error that caused all of our fNL values to be off by a factor of h^2. Our conclusions (and nearly 100% of the text) are unchanged because they were all in reference to the probability of fNL > 0 and the relative effect of systematics on the recovered constraint

    High-density marker profiling confirms ancestral genomes of Avena species and identifies D-genome chromosomes of hexaploid oat

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    We investigated genomic relationships among 27 species of the genus Avena using high-density genetic markers revealed by genotyping-by-sequencing (GBS). Two methods of GBS analysis were used: one based on tag-level haplotypes that were previously mapped in cultivated hexaploid oat (A. sativa), and one intended to sample and enumerate tag-level haplotypes originating from all species under investigation. Qualitatively, both methods gave similar predictions regarding the clustering of species and shared ancestral genomes. Furthermore, results were consistent with previous phylogenies of the genus obtained with conventional approaches, supporting the robustness of whole genome GBS analysis. Evidence is presented to justify the final and definitive classification of the tetraploids A. insularis, A. maroccana (=A. magna), and A. murphyi as containing D-plus-C genomes, and not A-plus-C genomes, as is most often specified in past literature. Through electronic painting of the 21 chromosome representations in the hexaploid oat consensus map, we show how the relative frequency of matches between mapped hexaploid-derived haplotypes and AC (DC)-genome tetraploids vs. A- and C-genome diploids can accurately reveal the genome origin of all hexaploid chromosomes, including the approximate positions of inter-genome translocations. Evidence is provided that supports the continued classification of a diverged B genome in AB tetraploids, and it is confirmed that no extant A-genome diploids, including A. canariensis, are similar enough to the D genome of tetraploid and hexaploid oat to warrant consideration as a D-genome diploid.publishersversionPeer reviewe

    Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species

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    Many herbicide-resistant weed species are polyploids, but far too little about the evolution of resistance mutations in polyploids is understood. Hexaploid wild oat (Avena fatua) is a global crop weed and many populations have evolved herbicide resistance. We studied plastidic acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide resistance in hexaploid wild oat and revealed that resistant individuals can express one, two or three different plastidic ACCase gene resistance mutations (Ile-1781-Leu, Asp-2078-Gly and Cys-2088-Arg). Using ACCase resistance mutations as molecular markers, combined with genetic, molecular and biochemical approaches, we found in individual resistant wild-oat plants that (1) up to three unlinked ACCase gene loci assort independently following Mendelian laws for disomic inheritance, (2) all three of these homoeologous ACCase genes were transcribed, with each able to carry its own mutation and (3) in a hexaploid background, each individual ACCase resistance mutation confers relatively low-level herbicide resistance, in contrast to high-level resistance conferred by the same mutations in unrelated diploid weed species of the Poaceae (grass) family. Low resistance conferred by individual ACCase resistance mutations is likely due to a dilution effect by susceptible ACCase expressed by homoeologs in hexaploid wild oat and/or differential expression of homoeologous ACCase gene copies. Thus, polyploidy in hexaploid wild oat may slow resistance evolution. Evidence of coexisting non-target-site resistance mechanisms among wild-oat populations was also revealed. In all, these results demonstrate that herbicide resistance and its evolution can be more complex in hexaploid wild oat than in unrelated diploid grass weeds. Our data provide a starting point for the daunting task of understanding resistance evolution in polyploids
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