Development of octopus aquaculture: Rearing, handling and systems design for Octopus tetricus commercial aquaculture FRDC Project No 2009/206

The following document ‘Development of octopus aquaculture, rearing, handling and systems designs for Octopus tetricus commercial aquaculture’ contains protocols developed during the FRDC project 2009/206. These protocols encompass the most up-to-date rearing, handling and systems designs for Octopus tetricus commercial aquaculture. These protocols are the result of extensive research and development work carried out over the past four years by the Department of Fisheries, Western Australia and summarised in the final project report

Development of octopus aquaculture Final Report FRDC Project No 2009/206

During the past four years, the marine aquaculture group at the WA Fisheries and Marine Research Laboratories (WAFMRL) in collaboration with Occoculture Pty Ltd (subsidiary of Fremantle Octopus) and as a part of the FRDC project ‘Octopus Aquaculture Development’, investigated the potential of octopus aquaculture in Western Australia. Hatchery methods were developed in an attempt to close the life cycle. Advanced systems, rearing and feeding protocols were established for ranching Octopus tetricus juveniles

La experiencia sensorial como estrategia de posicionamiento del restaurante Dpaso, Lima, 2020

Esta investigación tuvo como objetivo analizar la experiencia sensorial en el posicionamiento del restaurante Dpaso, Lima, 2020. Cuyo enfoque fue cualitativo, de tipo aplicada con diseño fenomenológico. Se realizó una entrevista de 14 preguntas distribuidas por cada categoría planteada a 14 comensales del restaurante Dpaso, entre los 25 a 70 años, la cuales fueron analizadas por el programa Atlas.ti. Los resultados indicaron que la combinación de colores está adaptada para crear una sensación de una hacienda de antaño; la música que predomina es la marinera y música criolla; los ambientes están bien distribuidos y no hay mezcla de olores; los sabores que poseen tienen un buen equilibrio gustativo; y las texturas más percibidas fueron el cuero y la madera, pero lo que más prevalece sobre las demás experiencias sensitivas fue tener contacto con los caballos. De esta manera se concluyó que la experiencia sensorial más predominante es la táctil, pues la interacción con los caballos es la cima de la experiencia sensorial en Dpaso, sin dejar de lado los otros sentidos pues crean un mix de experiencias sensoriales que resultan ser muy agradables y satisfactorios para los comensales, y que pueden ser utilizadas como estrategias de posicionamiento

Role of Diffusion Tensor Imaging in Prognostication and Treatment Monitoring in Niemann-Pick Disease Type C1

Niemann-Pick Disease, type C1 (NPC1) is a rapidly progressive neurodegenerative disorder characterized by cholesterol sequestration within late endosomes and lysosomes, for which no reliable imaging marker exists for prognostication and management. Cerebellar volume deficits are found to correlate with disease severity and diffusion tensor imaging (DTI) of the corpus callosum and brainstem, which has shown that microstructural disorganization is associated with NPC1 severity. This study investigates the utility of cerebellar DTI in clinical severity assessment. We hypothesize that cerebellar volume, fractional anisotropy (FA) and mean diffusivity (MD) negatively correlate with NIH NPC neurological severity score (NNSS) and motor severity subscores. Magnetic resonance imaging (MRI) was obtained for thirty-nine NPC1 subjects, ages 1–21.9 years (mean = 11.1, SD = 6.1). Using an atlas-based automated approach, the cerebellum of each patient was measured for FA, MD and volume. Additionally, each patient was given an NNSS. Decreased cerebellar FA and volume, and elevated MD correlate with higher NNSS. The cognition subscore and motor subscores for eye movement, ambulation, speech, swallowing, and fine motor skills were also statistically significant. Microstructural disorganization negatively correlated with motor severity in subjects. Additionally, Miglustat therapy correlated with lower severity scores across ranges of FA, MD and volume in all regions except the inferior peduncle, where a paradoxical effect was observed at high FA values. These findings suggest that DTI is a promising prognostication tool

Associations between anxious-depressed symptoms and cardiovascular risk factors in a longitudinal childhood study

Objective.To examine the influence of anxious/depressed scores on cardiovascular risk factors throughout childhood. Methods. Data from the Western Australian Pregnancy Cohort (Raine) Study, a study of 2900 pregnancies recruited between 1989 and 1991, were used. Anxious-depressed scores (derived from the Childhood Behavior Checklist), body mass index (BMI) and blood pressure were measured at 5 (n=1681), 8 (n=1697), 10 (n=1575) and 14 (n=1386) years. At age 14 depressive symptom scores (Beck Depression Inventory for Youth), anxious-depressed scores (Youth Self-Report (YSR) and Teacher Report Form (TRF)) and fasting lipid, glucose and insulin were also available. Cross sectional and longitudinal analyses were conducted. Results.At age 14, girls with higher anxious-depressed scores had higher BMI (p≤0.005) and homeostasis model assessment-estimated insulin resistance (p≤0.0001). This equated to a difference of 0.6 kg/m2 and 0.3units in predicted BMI and HOMA-IR respectively (top 5% vs. score of zero). Boys with higher anxiousdepressed scores had lower systolic blood pressure trajectories (p=0.024).Conclusion.Depressive scores appear to have differing influences on BMI, homeostasis model assessmentestimated insulin resistance and systolic blood pressure in boys and girls. Paradoxically boys with higher anxious-depressed scores had lower blood pressure throughout childhood

GOBIKY

El proyecto que lleva el nombre de GoBiky demuestra la viabilidad mediante el desarrollo de una aplicación que tiene como función brindar la mejor ruta disponible para transitar con su bicicleta y forma que se ahorre tiempo y le brinde seguridad, para clientes de ambos sexos pertenecientes a los NSE A, B y C. Además, a causa de la pandemia COVID-19 muchas personas decidieron apartarse de los medios tradicionales de transporte (taxi o microbús) para empezar a usar sus bicicletas o comprar una para usarlas ya que es un método muy efectivo para no interactuar con otras personas. Para la realización del proyecto se hizo un análisis del mercado que ayudaron a conocer los componentes que conforman parte de la estructura del mercado para el proyecto con el motivo de determinar su viabilidad de GoBiky como aplicación. Parte de estos demuestras las necesidades que el cliente tiene, sus intenciones de compra y la forma en cómo llegar a ellos con estrategias de marketing.The project that bears the name of GoBiky demonstrates the viability through the development of an application whose function is to provide the best available route to travel with your bicycle and a way that saves time and provides security, for clients of both sexes belonging to the NSE A, B and C. In addition, due to the COVID-19 pandemic, many people decided to move away from traditional means of transportation (taxi or minibus) to start using their bicycles or to buy one to use them since it is a very effective method not to interact with other people. To carry out the project, a market analysis which helped to know the components that make up part of the market structure for the project in order to determine the viability of GoBiky as an application. Part of these shows the nonsense that the customer has, their purchase intentions and the way to reach them with marketing strategies.Trabajo de investigació

Eight common genetic variants associated with serum dheas levels suggest a key role in ageing mechanisms

Dehydroepiandrosterone sulphate (DHEAS) is the most abundant circulating steroid secreted by adrenal glands-yet its function is unknown. Its serum concentration declines significantly with increasing age, which has led to speculation that a relative DHEAS deficiency may contribute to the development of common age-related diseases or diminished longevity. We conducted a meta-analysis of genome-wide association data with 14,846 individuals and identified eight independent common SNPs associated with serum DHEAS concentrations. Genes at or near the identified loci include ZKSCAN5 (rs11761528; p = 3.15×10-36), SULT2A1 (rs2637125; p = 2.61×10-19), ARPC1A (rs740160; p = 1.56×10-16), TRIM4 (rs17277546; p = 4.50×10-11), BMF (rs7181230; p = 5.44×10-11), HHEX (rs2497306; p = 4.64×10-9), BCL2L11 (rs6738028; p = 1.72×10-8), and CYP2C9 (rs2185570; p = 2.29×10-8). These genes are associated with type 2 diabetes, lymphoma, actin filament assembly, drug and xenobiotic metabolism, and zinc finger proteins. Several SNPs were associated with changes in gene expression levels, and the related genes are connected to biological pathways linking DHEAS with ageing. This study provides much needed insight into the function of DHEAS

Genome-wide association and HLA fine-mapping studies identify risk loci and genetic pathways underlying allergic rhinitis

Allergic rhinitis is the most common clinical presentation of allergy, affecting 400 million people worldwide, with increasing incidence in westernized countries1,2. To elucidate the genetic architecture and understand the underlying disease mechanisms, we carried out a meta-analysis of allergic rhinitis in 59,762 cases and 152,358 controls of European ancestry and identified a total of 41 risk loci for allergic rhinitis, including 20 loci not previously associated with allergic rhinitis, which were confirmed in a replication phase of 60,720 cases and 618,527 controls. Functional annotation implicated genes involved in various immune pathways, and fine mapping of the HLA region suggested amino acid variants important for antigen binding. We further performed genome-wide association study (GWAS) analyses of allergic sensitization against inhalant allergens and nonallergic rhinitis, which suggested shared genetic mechanisms across rhinitis-related traits. Future studies of the identified loci and genes might identify novel targets for treatment and prevention of allergic rhinitis