643 research outputs found
Clinical problem solving: Maneuvering around a narrow complex tachycardia in a patient with Mustard repair for transposition of the great arteries
Variables in the Southern Polar Region Evryscope 2016 Dataset
The regions around the celestial poles offer the ability to find and
characterize long-term variables from ground-based observatories. We used
multi-year Evryscope data to search for high-amplitude (~5% or greater)
variable objects among 160,000 bright stars (Mv < 14.5) near the South
Celestial Pole. We developed a machine learning based spectral classifier to
identify eclipse and transit candidates with M-dwarf or K-dwarf host stars -
and potential low-mass secondary stars or gas giant planets. The large
amplitude transit signals from low-mass companions of smaller dwarf host stars
lessens the photometric precision and systematics removal requirements
necessary for detection, and increases the discoveries from long-term
observations with modest light curve precision. The Evryscope is a robotic
telescope array that observes the Southern sky continuously at 2-minute
cadence, searching for stellar variability, transients, transits around exotic
stars and other observationally challenging astrophysical variables. In this
study, covering all stars 9 < Mv < 14.5, in declinations -75 to -90 deg, we
recover 346 known variables and discover 303 new variables, including 168
eclipsing binaries. We characterize the discoveries and provide the amplitudes,
periods, and variability type. A 1.7 Jupiter radius planet candidate with a
late K-dwarf primary was found and the transit signal was verified with the
PROMPT telescope network. Further followup revealed this object to be a likely
grazing eclipsing binary system with nearly identical primary and secondary K5
stars. Radial velocity measurements from the Goodman Spectrograph on the 4.1
meter SOAR telescope of the likely-lowest-mass targets reveal that six of the
eclipsing binary discoveries are low-mass (.06 - .37 solar mass) secondaries
with K-dwarf primaries, strong candidates for precision mass-radius
measurements.Comment: 32 pages, 17 figures, accepted to PAS
Using Raman Spectroscopy to Improve Hyperpolarized Noble Gas Production for Clinical Lung Imaging Techniques
Spin-exchange optical pumping (SEOP) can be used to âhyperpolarizeâ 129Xe for human lung MRI. SEOP involves transfer of angular momentum from light to an alkali metal (Rb) vapor, and then onto 129Xe nuclear spins during collisions; collisions between excited Rb and N2 ensure that incident optical energy is nonradiatively converted into heat. However, because variables that govern SEOP are temperature-dependent, the excess heat can complicate efforts to maximize spin polarizationâparticularly at high laser fluxes and xenon densities. Ultra-low frequency Raman spectroscopy may be used to perform in situ gas temperature measurements to investigate the interplay of energy thermalization and SEOP dynamics. Experimental configurations include an âorthogonalâ pump-and-probe design and a newer âinlineâ design (with source and detector on the same axis) that has provided a >20-fold improvement in SNR. The relationship between 129Xe polarization and the spatiotemporal distribution of N2 rotational temperatures has been investigated as a function of incident laser flux, exterior cell temperature, and gas composition. Significantly elevated gas temperatures have been observedâhundreds of degrees hotter than exterior cell surfacesâand variances with position and time can indicate underlying energy transport, convection, and Rb mass-transport processes that, if not controlled, can negatively impact 129Xe hyperpolarization
DNA variation and brain region-specific expression profiles exhibit different relationships between inbred mouse strains: implications for eQTL mapping studies
BACKGROUND: Expression quantitative trait locus (eQTL) mapping is used to find loci that are responsible for the transcriptional activity of a particular gene. In recent eQTL studies, expression profiles were derived from either homogenized whole brain or collections of large brain regions. However, the brain is a very heterogeneous organ, and expression profiles of different brain regions vary significantly. Because of the importance and potential power of eQTL studies in identifying regulatory networks, we analyzed gene expression patterns in different brain regions from multiple inbred mouse strains and investigated the implications for the design and analysis of eQTL studies. RESULTS: Gene expression profiles of five brain regions in six inbred mouse strains were studied. Few genes exhibited a significant strain-specific expression pattern, whereas a large number of genes exhibited brain region-specific patterns. We constructed phylogenetic trees based on the expression relationships between the strains and compared them with a DNA-level relationship tree. The trees based on the expression of strain-specific genes were constant across brain regions and mirrored DNA-level variation. However, the trees based on region-specific genes exhibited a different set of strain relationships, depending on the brain region. An eQTL analysis showed enrichment of cis-acting regulators among strain-specific genes, whereas brain region-specific genes appear to be mainly regulated by trans-acting elements. CONCLUSION: Our results suggest that many regulatory networks are highly brain region specific and indicate the importance of conducting eQTL mapping studies using data from brain regions or tissues that are physiologically and phenotypically relevant to the trait of interest
Multiplexed droplet Interface bilayer formation
We present a simple method for the multiplexed formation of droplet interface bilayers (DIBs) using a mechanically operated linear acrylic chamber array. To demonstrate the functionality of the chip design, a lipid membrane permeability assay is performed. We show that multiple, symmetric DIBs can be created and separated using this robust low-cost approach
Sculpting and fusing biomimetic vesicle networks using optical tweezers
Constructing higher-order vesicle assemblies has discipline-spanning potential from responsive soft-matter materials to artificial cell networks in synthetic biology. This potential is ultimately derived from the ability to compartmentalise and order chemical species in space. To unlock such applications, spatial organisation of vesicles in relation to one another must be controlled, and techniques to deliver cargo to compartments developed. Herein, we use optical tweezers to assemble, reconfigure and dismantle networks of cell-sized vesicles that, in different experimental scenarios, we engineer to exhibit several interesting properties. Vesicles are connected through double-bilayer junctions formed via electrostatically controlled adhesion. Chemically distinct vesicles are linked across length scales, from several nanometres to hundreds of micrometres, by axon-like tethers. In the former regime, patterning membranes with proteins and nanoparticles facilitates material exchange between compartments and enables laser-Triggered vesicle merging. This allows us to mix and dilute content, and to initiate protein expression by delivering biomolecular reaction components
Rheological droplet interface bilayers (rheo-DIBs): Probing the unstirred water layer effect on membrane permeability via spinning disk induced shear stress
A new rheological droplet interface bilayer (rheo-DIB) device is presented as a tool to apply shear stress on biological lipid membranes. Despite their exciting potential for affecting high-throughput membrane translocation studies, permeability assays conducted using DIBs have neglected the effect of the unstirred water layer (UWL). However as demonstrated in this study, neglecting this phenomenon can cause significant underestimates in membrane permeability measurements which in turn limits their ability to predict key processes such as drug translocation rates across lipid membranes. With the use of the rheo-DIB chip, the effective bilayer permeability can be modulated by applying shear stress to the droplet interfaces, inducing flow parallel to the DIB membranes. By analysing the relation between the effective membrane permeability and the applied stress, both the intrinsic membrane permeability and UWL thickness can be determined for the first time using this model membrane approach, thereby unlocking the potential of DIBs for undertaking diffusion assays. The results are also validated with numerical simulations
EVR-CB-001: An evolving, progenitor, white dwarf compact binary discovered with the Evryscope
We present EVR-CB-001, the discovery of a compact binary with an extremely
low mass () helium core white dwarf progenitor (pre-He
WD) and an unseen low mass () helium white dwarf (He
WD) companion. He WDs are thought to evolve from the remnant helium-rich core
of a main-sequence star stripped during the giant phase by a close companion.
Low mass He WDs are exotic objects (only about .2 of WDs are thought to be
less than .3 ), and are expected to be found in compact binaries.
Pre-He WDs are even rarer, and occupy the intermediate phase after the core is
stripped, but before the star becomes a fully degenerate WD and with a larger
radius () than a typical WD. The primary component of
EVR-CB-001 (the pre-He WD) was originally thought to be a hot subdwarf (sdB)
star from its blue color and under-luminous magnitude, characteristic of sdBs.
The mass, temperature (), and surface gravity
() solutions from this work are lower than values for
typical hot subdwarfs. The primary is likely to be a post-RGB, pre-He WD
contracting into a He WD, and at a stage that places it nearest to sdBs on
color-magnitude and - diagrams. EVR-CB-001 is expected to
evolve into a fully double degenerate, compact system that should spin down and
potentially evolve into a single hot subdwarf star. Single hot subdwarfs are
observed, but progenitor systems have been elusive.Comment: 14 pages, 11 figures. Published in The Astrophysical Journa
The First Triple-Decker Complex with a Carbenium Center, [CpCo(Ό-C3B2Me5)RuC5Me4CH2]+: Synthesis, Reactivity, X-Ray Structure, and Bonding
The first derivative of the methylium cation with the triple-decker substituent, [CpCo(C3B2Me5)RuC5Me4CH2]PF6 ( 2 PF6), was synthesized from the reaction of the triple-decker complex CpCo(C3B2Me5)RuCp* ( 1 ) with the salt of the trityl cation [CPh3]+. The X-ray crystal structure of 2 PF6 reveals that the methylium carbon is bound to the ruthenium with RuâC bond length of 2.259â
Ă
and corresponds to the description of its structure as η6-fulvene-ruthenium. Reactions of 2 PF6 with nucleophiles OHâ, Ph3P, Et3N led to the corresponding derivatives of 1 in high yields. Aromatic amines PhNEt2 and 4-MeC6H4NH2 react with 2 PF6 to give the electrophilic aromatic substitution products quantitatively. Chemical reduction of 2 PF6 with Zn powder in tetrahydrofuran leads to the formation of the bis(triple-decker) derivative (CpCo(C3B2Me5)RuC5Me4CH2)2 ( 10 ) with a CH2CH2-bridge. The structures of complexes 4 , 7 â 10 were determined by X-ray diffraction. Density functional calculations support the crystallographically determined geometry of 2 and allow rationalization of some characteristics of its structure, spectroscopy, and reactivity
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