Willingness to pay for treatment with highly active antiretroviral (HAART) drugs: a rural case study in Cameroon

This paper reports on the willingness of HIV/AIDS patients to pay for the most affordable triple therapy combination of antiretrovirals in a local setting in Cameroon. Questionnaires were used to evaluate willingness to pay, and patients who could still afford their medication 6 months after the survey were also investigated, to give an indication of actual ability to pay. In addition, oral interviews were carried out for clarification. In all, 84 patients out of a total of 186 were involved in the study. Results indicated that more men (39%) were willing to pay than women (22%), although more women (56%) were afflicted than men.Willingness to pay was directly dependent on cost with 69%, 22% and 9% of respondents indicating willingness to pay $1,$2 and $3 a day respectively. After 6 months of treatment, 22SAHARA-J (2004) 1(2): 107-113 Keywords: HIV/AIDS, treatment, access to antiretrovirals, drug costs, affordability. RÉSUMÉ Cette communication porte sur une bonne volonté des patients souffrants du VIH/SIDA à payer un prix assez abordable de la thérapie triple des anti-rétroviraux dans un milieu rural au Cameroun. Des questionnaires ont été utilisés afin d'évaluer cette bonne volonté à payer les médicaments. De plus, les patients qui avaient les moyens d'acheter leurs médicaments 6 mois après ces épreuves furent interviewés également. En plus de cela, les patients ont été interviewés avec le but d'obtenir des éclaircissements. Parmi les 186 patients, 84 participaient à l'étude. Les résultats de cette étude ont démontré que davantage d'hommes (39$1, $2 et$3 par jour respectivement. Au bout d'une période de 6 mois de traitement, 22% de patients étaient encore en thérapie. Une majorité de patients ont arrêté le traitement au bout de 6 mois faute de manque d'argent. En dehors du coût, le stigmate, l'incrédulité et les effets secondaires des médicaments étaient les facteurs principaux qui étaient contre la volonté de payer. Il est conseillé d'améliorer les services de consultation, de fournir de l'information, de réduire le coût de médicaments ainsi que les essais en laboratoire et l'élaboration des programmes de déstigmatisation afin d'améliorer la capacité de patients à payer pour les anti-rétroviraux. SAHARA-J (2004) 1(2): 107-113 Mots clés: le VIH/SIDA, le traitement, l'accès aux anti-rétroviraux, le coût de médicaments, avoir des moyens d'acheter

Preventing mother-to-child transmission: factors affecting mothers\' choice of feeding — a case study from Cameroon

This paper reports on factors influencing the decision of mothers regarding the type of feeding method for their babies in a rural setting in Cameroon. The aim of the study was to ascertain the proportion of mothers choosing the different methods of feeding, to determine the various factors influencing their choices, and to ascertain the relationships of these factors to their respective choices. Questionnaires were used on 108 HIV-positive mothers who had delivered babies and who were administered nevirapine at least 3 months prior to the study. A focus group discussion with mothers also took place. Findings were that more mothers (84%) chose breastfeeding than artificial feeding (16%), while a minority (4%) selected mixed feeding. Factors found to militate against artificial feeding were cost (69%), stigma (64%), family pressure (44%), inconvenience in preparation/administration (38%), prior education from health workers (23%), and loss of special attention from family (8%). On the other hand, advice of health worker (44%), ill health (19.5%), free milk (12.5%), job pressure (12.5%) and loss of beauty (12.5%) were found to militate against breastfeeding. A direct relationship was also found between age, educational level, income size, marital status and choice of feeding. Policies targeting stigma reduction and socio-cultural factors affecting the choice of feeding are needed to optimise uptake of the less risky methods of feeding which could in turn contribute to a reduction in transmission. Key words: HIV/AIDS, mother-to-child transmission prevention, feeding. La prévention de la transmission mère-enfant: les facteurs influençant le choix de la mère sur la méthode d'allaitement – une etude de cas du Cameroun RÉSUMÉ Cette communication présente des facteurs qui influencent la décision des mères concernant la méthode d\'allaitement de leur nourrissons, en zone rurale au Cameroun. Le but de cette étude était de s\'assurer de la proportion de mères qui choisissent de méthodes différentes d\'allaitement, de déterminer les facteurs influençant leur choix et d\'étudier la relation entre ces facteurs et les méthodes d\'allaitement choisies. Pour recueillir des données, un questionnaire a été utilisé auprès de 108 mères séropositives qui ont des nourrissons. Ces mères étaient sur le traitement de la névirapine depuis au moins 3 mois avant le début de cette étude. Une discussion d\'un groupe de foyer de mères a eu lieu. Les résultats ont montré que plus de 84% de mères ont choisi l\'allaitement maternel contre 16% qui ont choisi l\'allaitement artificiel alors qu\'une minorité (4%) ont choisi l\'allaitement mixte (maternel et artificiel). Les raisons contre l\'allaitement artificiel sont les suivants: le coût (69%); la stigmatisation (64%); les pressions familiales (44%); les inconvénients liés à la préparation et l\'administration du lait artificiel (38%); une éducation préliminaire de la part du personnel soignant (23%) et le manque de soins particuliers de la part de la famille (8%). D\'autre part, les facteurs qui favorisent l\'allaitement artificiel sont les suivants: les conseils du personnel de santé (44%); la mauvaise santé (19.5%); du lait artificiel gratuit (12.5%) et la perte de beauté (12.5%). Nous avons constaté une relation entre l\'âge de la femme, le niveau d\'éducation, le niveau du revenu, le statut civil et le choix d\'allaitement. Il est nécessaire de mettre en place des politiques visant la réduction de la stigmatisation et les facteurs socioculturels qui influencent le choix d\'une méthode d\'allaitement afin de maximiser une compréhension de méthodes d\'allaitement à moindre risque qui par la suite pourrait contribuer à la réduction de transmission. Mots clés :VIH/SIDA, prévention de la transmission mère-enfant, allaitement. Sahara J Vol.1(3) 2004: 132-13

Training workshop report on good agricultural practices for the production and conservation of seed potato in the West, Adamawa, and Northwest regions. Workshop report.

The International Potato Center (CIP), in collaboration with the Green Innovation Centers for the Food and Agriculture sector (ProCISA), the Ministry of Agriculture and Rural Development (MINADER), and the Institute of Agricultural Research for Development (IRAD), organized and facilitated training workshops for seed growers on Good Agricultural Practices (GAPs) for the production and conservation of seed potato. The workshops that took place in ADYS hotel in Dschang on 26 – 27 April 2021, TRANSCAM hotel in Ngaoundere on 5 – 7 May 2021, ADYS Hotel in Dschang on 9 -11 August 2021, brought together active and potential seed growers from the West, Adamawa, and Northwest regions, respectively. Seed growers from Lebialem division (South-West region) were also invited to join the Northwest cohort. The aim of the workshops was to share the knowledge with participants on Good Agricultural Practices for the production and conservation of seed potato. The workshops were moderated by the regional MINADER – ProCISA Focal Points and facilitated by CIP and IRAD researchers, as well as representatives of the Directorate of Regulation and Quality Control of Agricultural Inputs and Products (DRCQ). In the Adamawa, a translator was brought on board to translate all the presentations and questions and answersinto the local language Fulfulde, given that participants could neither express themselves in the French language nor understand it. This was an opportunity for participants in the three regions to (i) identify the constraints they face in seed production and conservation, (ii) know about the standard seed and variety schemes, (iii) learn GAPs for the production and conservation of seed potato, (iv) and identify their individual needs and define individual action plans to improve their agribusiness in seed potato. The theoretical sessions were conducted in a participatory manner combining PowerPoint presentations, questions, and answers as well as discussions and sharing of personal experiences. Field demonstrations were equally carried out during which participants were drilled on the identification of pests and diseases, positive and negative selection, dehaulming, phytosanitary treatment, and the proper handling of phytosanitary products, amongst others. Participants in the workshops were either active seed potato growers or those aspiring to venture into seed potato production. In the West region, fifteen (15) participants attended, of whom only one (01) was a woman. In the Adamawa region, eighteen (18) participants took part, of whom six (06) were women. For the Northwest region and Lebialem division, twenty-four (24) participants attended the training, of whom six (06) were women. At the end of the workshops, trainees were satisfied with the knowledge acquired and expressed the need to be accompanied for bettering the quality and quantity of their seed in their respective regions and divisions

Assessing the performance of a Fasciola gigantica serum antibody ELISA to estimate prevalence in cattle in Cameroon

BACKGROUND:Cattle rearing in Cameroon is both economically and culturally important, however parasitic diseases detrimentally impact cattle productivity. In sub-Saharan Africa bovine fasciolosis is generally attributed to F. gigantica, although understanding of Fasciola species present and local epidemiology in individual countries is patchy. Partly limited by the lack of representative surveys and understanding of diagnostic test perfromance in local cattle populations. The aims of this paper were to determine the Fasciola species infecting cattle, develop a species specific serum antibody ELISA, assess the performance of the ELISA and use it to assess the prevalence of F. gigantica exposure in two important cattle-rearing areas of Cameroon. RESULTS:A random sample of Fasciola parasites were collected and were all identified as F. gigantica (100%, CI:94.0-100%, n = 60) using RAPD-PCR analysis. A F. gigantica antibody ELISA was developed and initially a diagnostic cut-off was determined using a sample of known positive and negative cattle. The initial cut-off was used as starting point to estimate an optimal cut-off to estimate the best combination of sensitivity and specificity. This was achieved through sampling a naturally infected population with known infection status (cattle slaughtered at Bamenda abattoir, North West Region (n = 1112) and Ngaoundere abattoir, Vina Division, Adamawa Region (n = 776) in Cameroon). These cattle were tested and results analysed using a Bayesian non-gold standard method. The optimal cut-off was 23.5, which gave a sensitivity of 65.3% and a specificity of 65.2%. The prevalence of exposure to F. gigantica was higher in cattle in Ngaoundere (56.4% CI: 50.2-60.0%) than Bamenda (0.6% CI: 0.0-1.4%). CONCLUSION:Fasciola gigantica was identified as the predominant Fasciola species in Cameroon. Although the sensitivity and specificity F. gigantica antibody ELISA requires improvement, the test has shown to be a potentially useful tool in epidemiological studies. Highlighting the need for better understanding of the impact of F. gigantica infections on cattle production in Cameroon to improve cattle production in the pastoral systems of Central-West Africa. This paper also highlights that non-gold standard latent class methods are useful for assessing diagnostic test performance in naturally-infected animal populations in resource limited settings

The evolution and phylodynamics of serotype A and SAT2 foot-and-mouth disease viruses in endemic regions of Africa

Abstract Foot-and-mouth disease (FMD) is a major livestock disease with direct clinical impacts as well as indirect trade implications. Control through vaccination and stamping-out has successfully reduced or eradicated the disease from Europe and large parts of South America. However, sub-Saharan Africa remains endemically affected with 5/7 serotypes currently known to be circulating across the continent. This has significant implications both locally for livestock production and poverty reduction but also globally as it represents a major reservoir of viruses, which could spark new epidemics in disease free countries or vaccination zones. This paper describes the phylodynamics of serotypes A and SAT2 in Africa including recent isolates from Cameroon in Central Africa. We estimated the most recent common ancestor for serotype A was an East African virus from the 1930s (median 1937; HPD 1922–1950) compared to SAT2 which has a much older common ancestor from the early 1700s (median 1709; HPD 1502–1814). Detailed analysis of the different clades shows clearly that different clades are evolving and diffusing across the landscape at different rates with both serotypes having a particularly recent clade that is evolving and spreading more rapidly than other clades within their serotype. However, the lack of detailed sequence data available for Africa seriously limits our understanding of FMD epidemiology across the continent. A comprehensive view of the evolutionary history and dynamics of FMD viruses is essential to understand many basic epidemiological aspects of FMD in Africa such as the scale of persistence and the role of wildlife and thus the opportunities and scale at which vaccination and other controls could be applied. Finally we ask endemic countries to join the OIE/FAO supported regional networks and take advantage of new cheap technologies being rolled out to collect isolates and submit them to the World Reference Laboratory

Burden of non-communicable disease studies in Europe: a systematic review of data sources and methodological choices

Background: Assessment of disability-adjusted life years (DALYs) resulting from non-communicable diseases (NCDs) requires specific calculation methods and input data. The aims of this study were to (i) identify existing NCD burden of disease (BoD) activities in Europe; (ii) collate information on data sources for mortality and morbidity; and (iii) provide an overview of NCD-specific methods for calculating NCD DALYs. Methods: NCD BoD studies were systematically searched in international electronic literature databases and in grey literature. We included all BoD studies that used the DALY metric to quantify the health impact of one or more NCDs in countries belonging to the European Region. Results: A total of 163 BoD studies were retained: 96 (59%) were single-country or sub-national studies and 67 (41%) considered more than one country. Of the single-country studies, 29 (30%) consisted of secondary analyses using existing Global Burden of Disease (GBD) results. Mortality data were mainly derived (49%) from vital statistics. Morbidity data were frequently (40%) drawn from routine administrative and survey datasets, including disease registries and hospital discharge databases. The majority (60%) of national BoD studies reported mortality corrections. Multimorbidity adjustments were performed in 18% of national BoD studies. Conclusion: The number of national NCD BoD assessments across Europe increased over time, driven by an increase in BoD studies that consisted of secondary data analysis of GBD study findings. Ambiguity in reporting the use of NCD-specific BoD methods underlines the need for reporting guidelines of BoD studies to enhance the transparency of NCD BoD estimates across Europe

Hundreds of variants clustered in genomic loci and biological pathways affect human height

Most common human traits and diseases have a polygenic pattern of inheritance: DNA sequence variants at many genetic loci influence the phenotype. Genome-wide association (GWA) studies have identified more than 600 variants associated with human traits, but these typically explain small fractions of phenotypic variation, raising questions about the use of further studies. Here, using 183,727 individuals, we show that hundreds of genetic variants, in at least 180 loci, influence adult height, a highly heritable and classic polygenic trait. The large number of loci reveals patterns with important implications for genetic studies of common human diseases and traits. First, the 180 loci are not random, but instead are enriched for genes that are connected in biological pathways (P = 0.016) and that underlie skeletal growth defects (P < 0.001). Second, the likely causal gene is often located near the most strongly associated variant: in 13 of 21 loci containing a known skeletal growth gene, that gene was closest to the associated variant. Third, at least 19 loci have multiple independently associated variants, suggesting that allelic heterogeneity is a frequent feature of polygenic traits, that comprehensive explorations of already-discovered loci should discover additional variants and that an appreciable fraction of associated loci may have been identified. Fourth, associated variants are enriched for likely functional effects on genes, being over-represented among variants that alter amino-acid structure of proteins and expression levels of nearby genes. Our data explain approximately 10% of the phenotypic variation in height, and we estimate that unidentified common variants of similar effect sizes would increase this figure to approximately 16% of phenotypic variation (approximately 20% of heritable variation). Although additional approaches are needed to dissect the genetic architecture of polygenic human traits fully, our findings indicate that GWA studies can identify large numbers of loci that implicate biologically relevant genes and pathways.

Methodological considerations in injury burden of disease studies across Europe: a systematic literature review

Background Calculating the disease burden due to injury is complex, as it requires many methodological choices. Until now, an overview of the methodological design choices that have been made in burden of disease (BoD) studies in injury populations is not available. The aim of this systematic literature review was to identify existing injury BoD studies undertaken across Europe and to comprehensively review the methodological design choices and assumption parameters that have been made to calculate years of life lost (YLL) and years lived with disability (YLD) in these studies. Methods We searched EMBASE, MEDLINE, Cochrane Central, Google Scholar, and Web of Science, and the grey literature supplemented by handsearching, for BoD studies. We included injury BoD studies that quantified the BoD expressed in YLL, YLD, and disability-adjusted life years (DALY) in countries within the European Region between early-1990 and mid-2021. Results We retrieved 2,914 results of which 48 performed an injury-specific BoD assessment. Single-country independent and Global Burden of Disease (GBD)-linked injury BoD studies were performed in 11 European countries. Approximately 79% of injury BoD studies reported the BoD by external cause-of-injury. Most independent studies used the incidence-based approach to calculate YLDs. About half of the injury disease burden studies applied disability weights (DWs) developed by the GBD study. Almost all independent injury studies have determined YLL using national life tables. Conclusions Considerable methodological variation across independent injury BoD assessments was observed; differences were mainly apparent in the design choices and assumption parameters towards injury YLD calculations, implementation of DWs, and the choice of life table for YLL calculations. Development and use of guidelines for performing and reporting of injury BoD studies is crucial to enhance transparency and comparability of injury BoD estimates across Europe and beyond

Total zinc intake may modify the glucose-raising effect of a zinc transporter (SLC30A8) variant: a 14-cohort meta-analysis.

OBJECTIVE: Many genetic variants have been associated with glucose homeostasis and type 2 diabetes in genome-wide association studies. Zinc is an essential micronutrient that is important for β-cell function and glucose homeostasis. We tested the hypothesis that zinc intake could influence the glucose-raising effect of specific variants. RESEARCH DESIGN AND METHODS: We conducted a 14-cohort meta-analysis to assess the interaction of 20 genetic variants known to be related to glycemic traits and zinc metabolism with dietary zinc intake (food sources) and a 5-cohort meta-analysis to assess the interaction with total zinc intake (food sources and supplements) on fasting glucose levels among individuals of European ancestry without diabetes. RESULTS: We observed a significant association of total zinc intake with lower fasting glucose levels (β-coefficient ± SE per 1 mg/day of zinc intake: -0.0012 ± 0.0003 mmol/L, summary P value = 0.0003), while the association of dietary zinc intake was not significant. We identified a nominally significant interaction between total zinc intake and the SLC30A8 rs11558471 variant on fasting glucose levels (β-coefficient ± SE per A allele for 1 mg/day of greater total zinc intake: -0.0017 ± 0.0006 mmol/L, summary interaction P value = 0.005); this result suggests a stronger inverse association between total zinc intake and fasting glucose in individuals carrying the glucose-raising A allele compared with individuals who do not carry it. None of the other interaction tests were statistically significant. CONCLUSIONS: Our results suggest that higher total zinc intake may attenuate the glucose-raising effect of the rs11558471 SLC30A8 (zinc transporter) variant. Our findings also support evidence for the association of higher total zinc intake with lower fasting glucose levels

Genome-wide profiling of humoral immunity and pathogen genes under selection identifies immune evasion tactics of Chlamydia trachomatis during ocular infection

The frequency and duration of Chlamydia trachomatis (Ct) ocular infections decrease with age, suggesting development of partial immunity. However, there is a lack of clear correlates of immunity to Ct infection in humans. We screened sera from a cohort of Gambian children followed for six-months against a Ct-proteome microarray. At genome sequence level, we detected signatures of selection from a population of ocular Ct isolates from Guinea-Bissau. Together these approaches allowed us to highlight the focus of humoral responses and hypothesise new modes of pathogen immune evasion. Children who were susceptible to frequent and/or prolonged Ct infection had a less focussed antibody response, including preferential recognition of forty-two antigens. There was evidence of positive and purifying selection across the genome, but little balancing selection. In contrast, most antigens that were associated with susceptibility were under neutral selection. These data suggest an evasion strategy in which Ct presents a large panel of irrelevant antigens to the immune system to block or misdirect protective responses. Development of a focused immune response, possibly induced through vaccination, may be an effective strategy to promote protection to Ct infection