Genetic Variation in Disease Resistance Against White Spot Syndrome Virus (WSSV) in Liptopenaeus vannamei

White Spot Syndrome Virus (WSSV) is the most damaging pathogen in terms of production and economic losses for the shrimp sector world-wide. Estimation of heritability for WSSV resistance was made in this study to obtain necessary parameter inputs for broadening the breeding objectives of an ongoing selective breeding programme for Whiteleg shrimp (Liptopenaeus vannamei) that has focussed exclusively on improving growth performance since 2014. The present study involved a disease challenge test experiment using a total of 15,000 shrimps from 150 full- and half-sib families (100 individuals per family). Survival rates were recorded at six different experimental periods: 1–3 days (S1), 1–5 days (S2), 1–7 days (S3), 1–9 days (S4), 1–12 days (S5), and 1–15 days (S6) and were used as measures of WSSV resistance. There was significant variation in WSSV resistance among families studied. Quantitative-real time PCR (qPCR) analysis showed that the amount of viral titer (viral load) was significantly lower in high than low resistance families. Analyses of heritability were carried out using linear mixed model (LMM) and threshold logistic generalized model (TLGM). Both linear and threshold models used showed that the heritability (h2) for WSSV resistance was moderate in the early infection phases (S1–S4), whilst a low h2 value was observed for survival after 12 and 15 days of the challenge test (S5 and S6). The transformed heritabilities for WSSV resistance ranged from 1 to 31% which were somewhat lower than those estimated on the liability scale. Genetic correlations between survival rates measured over six different days post-infection were high and positive (0.82–0.99). The phenotypic correlations ranged from 0.31 ± 0.01 to 0.97 ± 0.01. The genetic correlations between body weights and WSSV resistance were negative. Our results on the heritability and genetic correlations show that improvement of WSSV resistance can be achieved through selective breeding in this population of Whiteleg shrimp

CHOLESTANE-TYPE STEROIDS FROM THE OCTOCORAL Verrucella corona

Using various chromatographic separations, three cholestane-type steroids were isolated from the methanol extract of the octocoral Verrucella corona. Their structures were elucidated to be (22E)-cholesta-5,22-dien-3β-ol-7-one (1), trans-liagosterol (2), and guggulsterol-II (3), by detailed analysis of the 1D and 2D-NMR data as well as comparison with those reported. Among them, compound 2 showed significant cytotoxicity against eight human cancer cell lines as HepG2, HL-60, KB, LNCaP, LU-1, MCF7, SK-Mel2, and SW480

Results of magnetotelluric survey for studying geothermal system in the Bang area, Quang Binh province

This paper presents the first results from the application of magnetotelluric method (MT) using the new equipment MTU 2000 (Canada) and analysis software to investigate the structure of geothermal area around the Bang hot water source (Quang Binh province). Results of data analysis by MT 1D and 2D models to a depth of 20 km show low resistivity zone in the southwest of Bang hot water (100°C) and allow for interpreting the structural elements of athehydro- geothermal system. This includes a very low resistivity layer at depth of 2 km suggesting a clay cap (heat resistive shield), a relatively low resistivity zone at depth ≥ 2 km reflecting  fractured rocks containing geothermal fluid and hot steam. A lower resistivity body at depth of 12-14 km located about 1.5 km from the hot water source indicates the existence of a heat source or a hot mass of intrusive magma., commonly thought to be sources of typical hydro- geothermal systems potential for energy exploitation. The obtained results not only provide new information for better understanding geothermal resource in the surveyed area, but also point out the methods and technology needed to improve the effectiveness for assessing potential of geothermal resources elsewhere in Vietnam.ReferencesBản đồ Địa chất và khoáng sản Việt Nam tỷ lệ 1:1.000.000. Cục Địa chất và Khoáng sản Việt Nam xuất bản 2004. Lưu trữ Địa chất. Cumming W., 2009: Geothermal resource conceptual models using surface exploration data. In: proceedings, 34th workshop on geothermal reservoir engineering, Stanford University. Data Processing User guide. Phoenix Geophysic Ltd. 2005, 201p. Di Pippo R., 2012: Geothermal Power plant. Principles, applications, case studies. 3rd edition. Elseverdirect, 579p. Doan Van Tuyen, Tran Anh Vu, Nguyen Thi Kim Thuong, 2014: Geochemical Characteristics of Geothermal Hot Water Sources on the Territory of Vietnam. Proceeding, Thirty-Eighth Workshop on Geothermal Reservoir Engineering Stanford University, Stanford, California, February 24-26, 2014 SGP-TR-202. Duchkov A.D., Nguyen Trong Yem, Dinh Van Toan, and Trinh Viet Bac, 1992: First estimations of heat flow in northern Vietnam. Soviet Geology and Geophysics, Vol. 33, No. 5, pp 92-96. Flynn T., Quy H. H., 1997: Assessment of the geothermal resources of Socialist Republic of Vietnam. Geothermal resources Council Transactions, vol.21, 341-345. IGA report, 2013: Geothermal Exploration best practices: A Guide to resource data collection, analysis, and presentation for Geothermal projects. He Lijuan, 1999: Analysis of heat flow along a transect across the South China Sea. Geothermal Training Programme, Reports 1999, Number 5, 125-140. Hoang Huu Quy, 1998: Overview of the Geothermal potential of Vietnam. Geothermics, Vol.27, n.1, 109-115. Koenig J. et al., 1981: Evaluation of the potential for Geothermal Energy Resources in the SR of Vietnam. Berkeley, CA. Kulinich G.G., Zabolotnikov A.A, Markov Yu., 1989: Cenozoic evalution of the Earth crust and orogeny in South- Eastern Asia (Tiếng Nga). MTU2000: User guide. Phoenix Geophysic Ltd. 2000, 36p. Munoz Gerard, 2014: Exploring for Geothermal Resources with Electromagnetic Methods. Surv Geophys (2014) 35:101-122, Springer, DOI 10.1007/s10712-013-9236-0. Pellerin et al., Johnston M, Hohmann W., 1996: A numerical evaluation of electromagnetic methods in geothermal exploration. Geophysics 61(1996):121-130. Thomas Mathews, et al., 2008: Study on the sozio-economic framework for the use Geothermal energy in Vietnam. Proceedings of the 8th Asian Geothermal Symposium, Hanoi. Trần Huyên, Trương Minh, Nguyễn Tiến Bào, 1999: Về chế độ địa nhiệt ở các bể trầm tích thềm lục địa Việt Nam. Tạp chí Kinh tế Địa chất và Nguyên liệu khoáng. Cục Địa chất và Khoáng sản Việt Nam. Số 18 tháng 2 năm 1999, tr.16-25. Võ Công Nghiệp (chủ biên), 1998: Danh bạ các nguồn nước khoáng và nước nóng Việt Nam. Cục Địa chất và Khoáng sản Việt Nam. Hà Nội, 300tr. Zhdanov M., 2009: Geophysical Electromagnetic Theory and Methods. Methods in Geochemistry and Geophysics, Volume 43. ISSN: 0076-6895 Elsevier, 831pp. WinGLink User guide. Geosystem 200, 182p. www.geosystem.net.

Prevalence and correlates of zinc deficiency in pregnant Vietnamese women in Ho Chi Minh City

Background: Although Vietnam is a region with a plant-based diet that has a high zinc deficiency, epidemiological data showing how this affects pregnant women are limited. This study explores the prevalence of zinc deficiency and possible correlates in pregnant Vietnamese women in Ho Chi Minh City. Methods: This was a crosssectional study conducted at a general hospital in Ho Chi Minh City, Vietnam. All pregnant women who came to their first antenatal care visit from November 2011 to June 2012 were recruited. Those taking a vitamin and/or mineral supplement were excluded. Serum zinc concentrations, determined by a standard colorimetric method, of 10.7 mol/L-17.5 mol/L (70.0 g/dL-114 g/dL) were classified as normal and under 10.7 mol/L (70.0 g/dL) as zinc deficient. Results: In total, 254 pregnant women were invited and 107 (42%) participated. The mean age of participants was 29 years, and mean gestational age was 10 weeks. Median zinc concentration in serum was 13.6 mol/L, and the prevalence of zinc deficiency was 29% (95% CI=21%-39%). The daily intake of a milk product supplement was the only significant correlate of zinc deficiency of the items investigated (adjusted OR=0.40, p=0.049). Discussion: This is the first study reporting that more than 25% of pregnant Vietnamese women in Ho Chi Minh City are zinc deficient. Further academic and clinical input is needed to confirm the scale of this neglected issue and to investigate the potential of milk product supplementation in this population

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke

Prospects for Genetic Improvement in Objective Measurements of Body Colour in Pacific Whiteleg Shrimp (Litopenaeus vannamei)

Body colour, together with growth and survival, are traits of commercial importance in Pacific whiteleg shrimp (Litopenaeus vannamei). However, heritability estimates for objective measurements of body colour are not available in Whiteleg shrimp species, including L. vannamei. Further, the effect of genotype by environment interactions (G &times; E) on this trait (i.e., the objective measures of body colour) and its genetic associations with growth are not known in this species. The present study presented the first attempt at understanding the genetic architecture of this complex character (body colour) that is of economic significance to the shrimp aquaculture sector world-wide. Specifically, we investigated the quantitative genetic basis of shrimp colour, while using the measurement tool (colorimeter) for a Whiteleg shrimp population reared in two contrasting environments. A total of 5464 shrimp had the objective measurements of body colour (lightness, yellowness, and redness) and growth trait records (weight, length and width). They were the offspring of 204 dams and 197 sires. The restricted maximum likelihood mixed model analysis showed that there were heritable additive genetic components for all of the measurements of shrimp colour, with the heritability (h2) ranging from 0.11&ndash;0.55. The h2 estimates for redness and yellowness traits differed between the two environments (h2 = 0.66&ndash;0.82 in Khanhhoa vs. 0.00&ndash;0.03 in Haiphong). However, the heritability for colour traits was moderate (0.11&ndash;0.55) when the two environments were combined. There is existence of (co)-genetic variances between the studied traits. The genetic correlations of body traits with redness or yellowness colour of the shrimp were moderate and positive (a*: 0.13&ndash;0.32 for redness and b*: 0.19&ndash;0.40 for yellowness). The effect of G &times; E interactions on shrimp colours could be important, as the genetic correlations for these traits between the two environments were low (&minus;0.41 to 0.16). Our results showed that the genetic improvement for body colour can be achieved through direct selection and the increased redness colour is also expected to have favorable impacts on growth traits. Breeding programs to improve shrimp colour should account for the effects of environmental factors

How Does Environmental Interpretation Affect Psychological Well-Being? A Study Conducted in the Context of COVID-19

The COVID-19 crisis has challenged and generated severe impact on the global society, economy, and environment. Under this pandemic context, governments and organizations around the world have issued and strengthened environmental policies and regulations to protect the environment and human health. However, the extant knowledge about how people’s interpretation of environmental policies and regulations influence their psychological well-being in the context of the COVID-19 pandemic is still limited. This study, therefore, investigates the impact of environmental interpretation on psychological well-being with the mediating role of environmentally responsible behavior and the moderating role of psychological contract violation. Using the data from a large sample of 960 residents in China, results of structural equation modeling show a positive relationship between environmental interpretation and psychological well-being, and this relationship is mediated by environmentally responsible behavior. Notably, psychological contract violation has a moderating effect on the indirect effect of environmental interpretation on psychological well-being via environmentally responsible behavior. These findings have several important implications for policymakers in environmental sustainability and pandemic planning