Decision Tree Analysis as a Supplementary Tool to Enhance Histomorphological Differentiation when Distinguishing Human from Non-human Cranial Bone in both Burnt and Unburnt States: A feasibility study

This feasibility study was undertaken to describe and record the histological characteristics of burnt and unburnt cranial bone fragments from human and non-human bones. Reference series of fully mineralised, transverse sections of cranial bone, from all variables and specimen states were prepared by manual cutting and semi-automated grinding and polishing methods. A photomicrograph catalogue reflecting differences in burnt and unburnt bone from human and non-humans was recorded and qualitative analysis was performed using an established classification system based on primary bone characteristics. The histomorphology associated with human and non-human samples was, for the main part, preserved following burning at high temperature. Clearly, fibro-lamellar complex tissue subtypes, such as plexiform or laminar primary bone, were only present in non-human bones. A decision tree analysis based on histological features provided a definitive identification key for distinguishing human from non-human bone, with an accuracy of 100%. The decision tree for samples where burning was unknown was 96% accurate, and multi-step classification to taxon was possible with 100% accuracy. The results of this feasibility study, strongly suggest that histology remains a viable alternative technique if fragments of cranial bone require forensic examination in both burnt and unburnt states. The decision tree analysis may provide an additional, but vital tool to enhance data interpretation. Further studies are needed to assess variation in histomorphology taking into account other cranial bones, ontogeny, species and burning conditions

Career Regret Among Physician Assistants: A Comparative Survey of Primary and Specialty Care Careers

Purpose: This study investigated if career regret varies among physician assistants (PAs) practicing in primary and specialty care fields. This information may assist practicing and aspiring physician assistants when selecting or changing their career path. Methods: A survey was emailed to 5,000 primary and specialty care physician assistants. Items indicating career regret were compared between primary and specialty care groups. Results: Eight hundred and thrity-four (16.7%) completed surveys were received back. Career regret is similar between primary and specialty care physician assistants, with low reports from both groups. No statistical significance was found between primary care and specialty care groups with regards to career regret or student loan debt. The primary care group noted a less sustainable work/ life balance and higher perceived burnout. Specialty care physician assistants reported higher annual gross income. Regret and disappointment correlated highly with burnout. Conclusions: Physician assistants and prospective physician assistants should carefully consider their career path as regret and disappointment correlated highly with burnout

Fractal light from lasers

Fractals, complex shapes with structure at multiple scales, have long been observed in Nature: as symmetric fractals in plants and sea shells, and as statistical fractals in clouds, mountains and coastlines. With their highly polished spherical mirrors, laser resonators are almost the precise opposite of Nature, and so it came as a surprise when, in 1998, transverse intensity cross-sections of the eigenmodes of unstable canonical resonators were predicted to be fractals [Karman et al., Nature 402, 138 (1999)]. Experimental verification has so far remained elusive. Here we observe a variety of fractal shapes in transverse intensity cross-sections through the lowest-loss eigenmodes of unstable canonical laser resonators, thereby demonstrating the controlled generation of fractal light inside a laser cavity. We also advance the existing theory of fractal laser modes, first by predicting 3D self-similar fractal structure around the centre of the magnified self-conjugate plane, second by showing, quantitatively, that intensity cross-sections are most self-similar in the magnified self-conjugate plane. Our work offers a significant advance in the understanding of a fundamental symmetry of Nature as found in lasers

Stock market integration for the transition economies: Time-varying conditional correlation approach

This is the accepted version of the following article: WANG, P. and MOORE, T. (2008), Stock market integration for the transition economies: Time-varying conditional correlation approach. The Manchester School, 76: 116–133. doi: 10.1111/j.1467-9957.2008.01083.x, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/j.1467- 9957.2008.01083.x/abstract.In this paper, we investigate the extent to which the three emerging Central Eastern European stock markets have become integrated with the aggregate eurozone market over the sample period from 1994 to 2006 by utilizing the dynamic conditional correlation. We find a higher level of the stock market correlation during the period after the Asian and Russian crises and also during the post-entry period to the European Union. It is found that financial market integration seems to be a largely self-fuelling process, depending on existing levels of financial sector development for the Czech Republic and Hungary

STATISTICAL ANALYSIS OF THE EFFECTS OF MIXING POTATO VARIETIES ON LATE BLIGHT

A field study in two regions of Peru was conducted to determine how host-diversity effects on potato late blight varied geographically. Foliar disease severity was evaluated separately for the potato varieties in mixtures as well as in the single-variety plots. The TAUDPC (truncated area under the disease progress curve) and RMR (relative mixture response) for each site were analyzed separately using SAS mixed effects model procedures. While there was little difference between the sites in the 1997-1998 season, host-diversity effects were generally greater near Huancayo than near Cajamarca in the 1998-1999 season. Estimates of host-diversity effects from studies in Oregon and Ecuador were also compared with results for Peru. Host-diversity effects for reduced disease were generally greater for sites where we predicted lower levels of outside inoculum

The Effects of Parental Behavior on Infants' Neural Processing of Emotion Expressions

Infants become sensitive to emotion expressions early in the 1st year and such sensitivity is likely crucial for social development and adaptation. Social interactions with primary caregivers may play a key role in the development of this complex ability. This study aimed to investigate how variations in parenting behavior affect infants' neural responses to emotional faces. Event-related potentials (ERPs) to emotional faces were recorded from 40 healthy 7-month-old infants (24 males). Parental behavior was assessed and coded using the Emotional Availability Scales during free-play interaction. Sensitive parenting was associated with increased amplitudes to positive facial expressions on the face-sensitive ERP component, the negative central. Findings are discussed in relation to the interactive mechanisms influencing how infants neurally encode positive emotions

Development of an invasively monitored porcine model of acetaminophen-induced acute liver failure

Background: The development of effective therapies for acute liver failure (ALF) is limited by our knowledge of the pathophysiology of this condition, and the lack of suitable large animal models of acetaminophen toxicity. Our aim was to develop a reproducible invasively-monitored porcine model of acetaminophen-induced ALF. Method: 35kg pigs were maintained under general anaesthesia and invasively monitored. Control pigs received a saline infusion, whereas ALF pigs received acetaminophen intravenously for 12 hours to maintain blood concentrations between 200-300 mg/l. Animals surviving 28 hours were euthanased. Results: Cytochrome p450 levels in phenobarbital pre-treated animals were significantly higher than non pre-treated animals (300 vs 100 pmol/mg protein). Control pigs (n=4) survived 28-hour anaesthesia without incident. Of nine pigs that received acetaminophen, four survived 20 hours and two survived 28 hours. Injured animals developed hypotension (mean arterial pressure; 40.8+/-5.9 vs 59+/-2.0 mmHg), increased cardiac output (7.26+/-1.86 vs 3.30+/-0.40 l/min) and decreased systemic vascular resistance (8.48+/-2.75 vs 16.2+/-1.76 mPa/s/m3). Dyspnoea developed as liver injury progressed and the increased pulmonary vascular resistance (636+/-95 vs 301+/-26.9 mPa/s/m3) observed may reflect the development of respiratory distress syndrome. Liver damage was confirmed by deterioration in pH (7.23+/-0.05 vs 7.45+/-0.02) and prothrombin time (36+/-2 vs 8.9+/-0.3 seconds) compared with controls. Factor V and VII levels were reduced to 9.3 and 15.5% of starting values in injured animals. A marked increase in serum AST (471.5+/-210 vs 42+/-8.14) coincided with a marked reduction in serum albumin (11.5+/-1.71 vs 25+/-1 g/dL) in injured animals. Animals displayed evidence of renal impairment; mean creatinine levels 280.2+/-36.5 vs 131.6+/-9.33 mumol/l. Liver histology revealed evidence of severe centrilobular necrosis with coagulative necrosis. Marked renal tubular necrosis was also seen. Methaemoglobin levels did not rise >5%. Intracranial hypertension was not seen (ICP monitoring), but there was biochemical evidence of encephalopathy by the reduction of Fischer's ratio from 5.6 +/- 1.1 to 0.45 +/- 0.06. Conclusion: We have developed a reproducible large animal model of acetaminophen-induced liver failure, which allows in-depth investigation of the pathophysiological basis of this condition. Furthermore, this represents an important large animal model for testing artificial liver support systems

Serological Responses of Raccoons and Striped Skunks to Ontario Rabies Vaccine Bait in West Virginia during 2012–2016

Since the 1990s, oral rabies vaccination (ORV) has been used successfully to halt the westward spread of the raccoon rabies virus (RV) variant from the eastern continental USA. Elimination of raccoon RV from the eastern USA has proven challenging across targeted raccoon (Procyon lotor) and striped skunk (Mephitis mephitis) populations impacted by raccoon RV. Field trial evaluations of the Ontario Rabies Vaccine Bait (ONRAB) were initiated to expand ORV products available to meet the rabies management goal of raccoon RV elimination. This study describes the continuation of a 2011 trial inWest Virginia. Our objective was to evaluate raccoon and skunk response to ORV occurring in West Virginia for an additional two years (2012–2013) at 75 baits/km2 followed by three years (2014–2016) of evaluation at 300 baits/km2. We measured the change in rabies virus-neutralizing antibody (RVNA) seroprevalence in targeted wildlife populations by comparing levels pre- and post-ORV during each year of study. The increase in bait density from 75/km2 to 300/km2 corresponded to an increase in average post-ORV seroprevalence for raccoon and skunk populations. Raccoon population RVNA levels increased from 53% (300/565, 95% CI: 50–57%) to 82.0% (596/727, 95% CI: 79–85%) during this study, and skunk population RVNA levels increased from 11% (8/72, 95% CI: 6–20%) to 39% (51/130, 95% CI: 31–48%). The RVNA seroprevalence pre-ORV demonstrated an increasing trend across study years for both bait densities and species, indicating that multiple years of ORV may be necessary to achieve and maintain RVNA seroprevalence in target wildlife populations for the control and elimination of raccoon RV in the eastern USA