Caracterización molecular y potencial zoonótico de aislados de Cryptosporidium en rumiantes domésticos y su relación con la edad

Para estudiar la presencia y especies de Cryptosporidium presentes en rumiantes domésticos sin signos digestivos en Galicia, se realizó un análisis genotípico mediante PCR-RFLP del gen 18S. En vacuno, C. parvum y C. bovis fueron más comunes que C. ryanae, C. andersoni y C. suis-like. Se apreció una asociación entre la edad de los bovinos y ciertas especies de Cryptosporidium, incrementándose su diversidad con los años. Se identificaron 2 subtipos zoonóticos de C. parvum (IIaA15G2R1 y IIaA16G3R1); por ello los bovinos suponen un riesgo de Salud Pública y para la aparición de diarreas neonatales. En pequeños rumiantes se identificaron C. parvum, C. xiaoi y C. ubiquitum. En ovinos, la prevalencia de C. parvum (IIaA14G2R1 y IIaA15G2R1) fue notable. En cabras, la prevalencia de C. parvum (IIaA13G1R1 y IIdA17G1) fue escasa, representando un menor riesgo de brotes de diarreas neonatales. La detección de los subtipos zoonóticos IIaA15G2R1 de C. parvum en ovinos y XIIa de C. ubiquitum en ovejas y cabras, confirma que en Galicia estos dos rumiantes pueden constituir una fuente de infección para las personas

Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Aim: To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay. Methodology: Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons. Results: The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%). Conclusions: The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution

Aproximación a los textos argumentativos a través de Twitter

El presente trabajo se enmarca en la modalidad A3 del Trabajo Final de Máster del Máster de formación del profesorado, especialidad en Lengua Castellana y Literatura e incluye el diseño de una secuencia didáctica, titulada “Mejor que levantar la voz, reforzar el argumento”, sobre la enseñanza de los textos argumentativos en tercero de la ESO. El enfoque que se le pretende dar a la enseñanza de este contenido es no solo el de conocer las características propias de estos textos, sino el de ser conscientes de la gran cantidad de textos argumentativos a los que nos enfrentamos y mejorar la capacidad de elaboración y análisis de estos. Para ello, se emplearán textos cercanos y reales para el alumnado, obtenidos a través de Twitter, ya que así se logrará generar interés, motivación y percepción de utilidad.<br /

Relationships between Strength and Step Frequency with Fatigue Index in Repeated Sprint Ability.

Force−velocity profile (FVP) and repeated-sprint ability (RSA) tests are indicators of physical capacities in most team sport players. The purpose of this study was to examine the stride kinematics during a repeated-sprint ability (RSA) test and to analyze the relationship between Bosco’s force−velocity profile (FVP) and RSA performance in elite female field hockey players. Thirteen elite-female players performed both RSA (six 30 m maximal sprints) and jumping (CMJ weighted and body weight) tests. Sprinting time fatigue indexes during a 30 m RSA test were correlated with step frequency fatigue indexes (r > 0.7; p 0.7; p 0.5; p < 0.05). This study highlighted two possible ways to improve fatigue indexes in RSA, with the aim of maximizing the distances covered at high-intensities during the matches: (a) strength training and (b) focusing on step frequency during speed training.post-print1622 K

Humidity forecasting in a potato plantation using time-series neural models

It is widely acknowledged that, under the frame of sustainable farming, using the minimum water resources is a relevant requirement. In order to do that, precision irrigation aims at identifying the irrigation needs of plantations and irrigate accordingly. Artificial intelligence is a promising solution in this field as intelligent models are able to learn the soil moisture dynamics in the soil-plant-atmosphere system and then generating appropriate irrigation scheduling. This is a complex task as the phenology of plants and its water demand vary with soil properties and weather conditions. The present research contributes to this challenging task by proposing the application of neural networks in order to learn the time-series evolution of irrigation needs associated to a potato plantation. Several of such models are thoroughly compared, together with different interpolation methods, in order to find the best combination for accurately forecasting water needs. In order to predict the soil water content in a potato field crop, in which soil humidity probes were installed at 15, 30, and 45 cm depth during the whole cycle of a potato crop. This innovative study and its promising results provide with significant contributions to address the problem of predicting and managing groundwater for agricultural use in a sustainable way.Lab-Ferrer (METER Group) and the UBUCOMP research group at the University of Burgos

Preferential Enhancement of Sensory and Motor Axon Regeneration by Combining Extracellular Matrix Components with Neurotrophic Factors

After peripheral nerve injury, motor and sensory axons are able to regenerate but inaccuracy of target reinnervation leads to poor functional recovery. Extracellular matrix (ECM) components and neurotrophic factors (NTFs) exert their effect on different neuronal populations creating a suitable environment to promote axonal growth. Here, we assessed in vitro and in vivo the selective effects of combining different ECM components with NTFs on motor and sensory axons regeneration and target reinnervation. Organotypic cultures with collagen, laminin and nerve growth factor (NGF)/neurotrophin-3 (NT3) or collagen, fibronectin and brain-derived neurotrophic factor (BDNF) selectively enhanced sensory neurite outgrowth of DRG neurons and motor neurite outgrowth from spinal cord slices respectively. For in vivo studies, the rat sciatic nerve was transected and repaired with a silicone tube filled with a collagen and laminin matrix with NGF/NT3 encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres (MP) (LM + MP.NGF/NT3), or a collagen and fibronectin matrix with BDNF in PLGA MPs (FN + MP.BDNF). Retrograde labeling and functional tests showed that LM + MP.NGF/NT3 increased the number of regenerated sensory neurons and improved sensory functional recovery, whereas FN + MP.BDNF preferentially increased regenerated motoneurons and enhanced motor functional recovery. Therefore, combination of ECM molecules with NTFs may be a good approach to selectively enhance motor and sensory axons regeneration and promote appropriate target reinnervation

Herramientas en la gestión y optimización de liderazgo en las tomas de decisiones

La presente investigación de tipo documental, tiene como tema la organización, y subtema las herramientas en la gestión y optimización de liderazgo en las tomas de decisiones. El objetivo general de esta investigación es analizar herramientas en la gestión y optimización de liderazgo en las tomas de decisiones, como comunicación gerencial para el éxito con competitividad en las organizaciones. La base teórica sustentada en este informe se presenta a través de cuatro capítulos tales como, capítulo uno: las generalidades de la gestión empresarial, en el capítulo dos se identifican los tipos de liderazgo, en el tercer capítulo se explica las habilidades directivas y su clasificación, un cuarto y último capítulo se definen las herramientas para la gestión y toma de decisiones para alcanzar el éxito. Las técnicas utilizadas para el desarrollo de este informe fueron la lectura y recolección de documentación bibliográfica basado en la administración estratégica, así mismo la tabulación del informe se hará aplicando las normas APAs 6 de Javeriano. Los resultados del informe están basados en la normativa de presentación de seminario de graduación como forma de culminación de los estudios del plan 2013 y 2016 de la UNAN- Managua. Los principales términos descriptores del informe son, resumen, introducción, justificación, objetivos, desarrollo del tema y subtema a través de los capítulos, conclusiones y bibliografía

Estereotipos hacia la vejez y su representación social

Introduction:Stereotypes are beliefs referred to characteristics or shared traits by members of specific group members and the typical traits or manners that are supposed by those who have who belong to this group.Objective:To identify the stereotypes towards the elderly period and their social representation in adolescents and adults.Methods: A descriptive cross-sectional study was carried out at Luis Li Trigent Teaching Policlinic in 2020. The universe was formed by 323 patients from the Doctor´s Office 13, from 10 to 49 years old, which were selected in a probabilistic way, at random 105 patients. The studied variables were age, sex, health stereotypes, social ones, character and the social representation. Absolute and relative frequency measures were applied, the Arithmetic media and the brain storm as a group technician.Results: In the 30 to 39 age group, female patients prevailed in a 21.90 %, the 31.42 % think that elderlies are fragile people, the 25.71 % appreciate them as sociable people and the 21.90 % as respectful people. The social representation showed different modalities directed to see the elderlies as people who need help and care.Conclusions: Women from 30 to 39 years old who see the elderlies as prevailed fragile, sociable respectful people prevail, the general representation of the elderly inquired adults is relatively positive, so it strengthens the idea that the active aging program favors the reconstruction of old age.Introducción:Los estereotipos son creencias referidas a características o rasgos compartidos por miembros de grupos sociales específicos y los rasgos típicos o modales que suponen poseen quienes pertenecen a estos grupos. Objetivo. Identificar los estereotipos hacia la vejez y su representación social en adolescentes y adultos.Métodos:Se realizó un estudio descriptivo transversal en el Policlínico Docente Luis Li Trigent en el año 2020. El universo quedó constituido por 323 pacientes del consultorio 13, en edades comprendidas entre 10 y 49 años del cual se seleccionaron de forma probabilística, al azar 105 pacientes. Se estudiaron variables como la edad, sexo, estereotipos de salud, sociales, de carácter y la representación social. Se aplicaron las medidas de frecuencia absolutas relativas, media aritmética y la lluvia de ideas como técnica grupal.Resultados:Predominaron las féminas en el grupo de 30 a 39 años en un 21.90 %, el 31.42 % piensa que los ancianos son personas frágiles, el 25.71% los ve como sociables y el 21.90 % como respetuosos. La representación social mostró diferentes matices dirigidas a ver los ancianos como personas que necesitan ayuda y cuidadoConclusiones: Predominan las mujeres de 30 a 39 años, que ven los ancianos como frágiles, sociables y respetuosos, la representación general del adulto mayor, es positiva de forma relativa, lo que refuerza la idea, de que el programa de envejecimiento activo, favorece la reconstrucción de la vejez

Antimicrobial activity and enterococcus faecalis biofilm formation on chlorhexidine varnishes

Objective: To evaluate, in vitro , the antimicrobial activity and biofilm formation of three chlorhexidine varnishes in four Enterococcus faecalis strains: E. faecalis ATCC 29212, E. faecalis EF-D1 (from failed endodontic treatment), E. faecalis 072 (cheese) and E. faecalis U-1765 (nosocomial infection), and one Enterococcus durans strain (failed endodontic treatment). Study Design: The direct contact test was used to study the antimicrobial activity. Bacterial suspensions were exposed for one hour to EC40, Cervitec (CE) and Cervitec Plus (CEP) varnishes. ?Eradication? was defined as 100% bacterial kill. The formation of enterococci biofilms was tested on the surface of the varnishes after 24 hours of incubation and expressed as percentage of biofilm reduction. Results: EC40 eradicated all strains except E. faecalis ATCC 29212, where 98.78% kill was achieved. CE and CEP showed antimicrobial activity against all the strains, but most clearly against E. durans and E. faecalis 072. EC40 completely inhibited the formation of biofilm of E. faecalis ATCC 29212, E. faecalis 072 and E. durans . CE and CEP led to over 92% of biofilm reduction, except in the case of E. faecalis U-1765 on CEP (76.42%). Conclusion: The three varnishes studied were seen to be effective in killing the tested strains of enterococci and in inhibiting the formation of biofilm, the best results being observed with EC40