La distribución anatómica de Amblyomma neumanni y Amblyomma parvum (Acari: Ixodidae) sobre sus hospedadores principales

En este trabajo se estudió la distribución anatómica de las garrapatas Amblyomma neumanni y Amblyomma parvum sobre sus hospedadores principales, que corresponden a bovinos para todos los estadios de A. neumanni y adultos de A. parvum, y al roedor Galea musteloides para larvas y ninfas de A. parvum. La fijación de A. neumanni abarcó varios sitios corporales; las larvas se fijaron principalmente sobre la cabeza, orejas, tronco y cuello, las ninfas sobre el tronco, cuello, pecho, cola y abdomen, y los adultos sobre la cabeza, tronco, cola, cuello, pecho, abdomen y ubre. Por el contrario, la preferencia de los tres estadios de A. parvum se restringió mayormente a la cabeza y orejas. La variación que se da naturalmente en el número de garrapatas fijadas tanto a bovinos como a G. musteloides de acuerdo a la estación del año, no produjo cambios significativos en la preferencia por un área corporal del hospedador.Fil: Nava, Santiago. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Agencia de Extension Rural Carlos Pellegrini.; ArgentinaFil: Mangold, A. J.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Agencia de Extension Rural Carlos Pellegrini.; ArgentinaFil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Agencia de Extension Rural Carlos Pellegrini.; Argentin

Everolimus, Cyclosporine, and Thrombotic Microangiopathy: Clinical Role and Preventive Tools in Renal Transplantation

INTRODUCTION: Thrombotic microangiopathy (TMA) is characterized by endothelial cell injury and formation of fibrin thrombi within capillary and arterioles. In renal allograft recipients, TMA mainly presents as hemolytic uremic syndrome. Its occurrence is rare, and diagnosis requires a high degree of suspicion. Drug toxicity, in particular from calcineurin inhibitors (CNIs) and mTOR inhibitors (mTORi), is the most common cause posttransplant and has recently been emphasized in the setting of lung transplantation. OBJECTIVE: The goal of this study was to investigate the role of mTORi as an added risk factor in the development of TMA to propose strategies for modulation of immunosuppressive (IS) therapy. PATIENTS AND METHODS: From a database of 496 renal graft recipients, we analyzed 350 renal graft biopsy specimens gathered at our center from 1998 to 2012. In patients undergoing combined therapy with mTORi and CNI, we compared drugs levels in TMA-affected and TMA-free groups, using mTORi and CNI TLC and the summation of [everolimus TLC+(cyclosporine C2/100)] (Σ) as a surrogate marker of combined exposition to 2 drugs. Receiver-operating characteristic analysis of association of EVL TLC+(C2/100) was performed for patients exposed to mTORi. RESULTS: Histologic features of TMA were found in 36 patients (prevalence of 7.3%). The caseload was divided into 2 groups: not drug-related TMA (n=19) and drug-related TMA (n=17). Despite the prevalence of TMA in patients exposed to mTORi being greater (8 of 153; prevalence, 5.3%) compared with therapies without mTORi (9 of 324; prevalence, 2.8%), statistical difference was not reached. Patients treated with mTORi who developed de novo drug-related TMA had higher blood levels of IS drugs compared with those who did not develop TMA. Receiver-operating characteristic analysis found a significant threshold of 12.5 ng/mL (area under the curve, 0.803; P=.006). CONCLUSIONS: Results confirm the pivotal role of IS drugs in the onset of de novo TMA. On the basis of literature, we could speculate a sequence of endothelial damage by CNI, on which everolimus fits hindering the repair of endothelial injury. Therefore, high blood levels of CNI and mTORi seem to predispose patients to posttransplant TMA. Combined monitoring of these 2 drugs might be used to prevent the complication. Σ [everolimus TLC + (cyclosporine C2/100)]>12.5 ng/mL should be avoided as a surrogate risk factor for adverse effects

Divergent environmental preferences and areas of sympatry of tick species in the Amblyomma cajennense complex (Ixodidae)

Four species of Neotropical ticks, Amblyomma mixtum, Amblyomma cajennense, Amblyomma tonelliae and Amblyomma sculptum (formerly included in the catch-all name A. cajennense), have an allopatric distribution in much of their range, with areas of parapatry for at least two of them. We inferred the abiotic niches of these organisms using coefficients of a harmonic regression of the temperature and the Normalized Difference Vegetation Index (NDVI, reflecting plant stress) from remotely sensed data from MODIS satellites with 0.05° spatial resolution. Combinations of coefficients describing the phenology of these two variables pointed to divergent niche preferences, compatible with previous events of vicariance among the species. Amblyomma cajennense has been recorded in areas with small variations in temperature and NDVI. The remaining species were recorded in areas with large variations. The maximum environmental niche overlap was ∼73.6% between A. mixtum and A. cajennense and 73.5% between A. tonelliae and A. sculptum. Projecting these inferences on the geographical space revealed probable areas of sympatry or parapatry between A. mixtum and A. cajennense or between A. tonelliae and A. sculptum, the latter of which was confirmed with field collections. The A. sculptum distribution overlaps with that of A. tonelliae in northern Argentina and Paraguay; parapatry occurs at one extreme of the conditions occupied by both species. Compared with areas of allopatry, sites with both species had consistently lower temperatures, except for 10–12 weeks during the summer, and higher NDVI values throughout the year. We hypothesise that the overlap between A. tonelliae and A. sculptum resulted from secondary contact between populations, with A. sculptum adapting to sites with high water availability to balance high summer temperatures. Additional surveys of the areas of spatial overlap among these species are necessary to elucidate the forces driving their evolution and their adaptation to the environment.Fil: Estrada Peña, Agustín. Universidad de Zaragoza; EspañaFil: Tarragona, Evelina Luisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Vesco, Umberto. Università di Torino; ItaliaFil: De Meneghi, Daniele. Università di Torino; ItaliaFil: Mastropaolo, Mariano. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentin

Exotic tick Amblyomma varanense (Acari: Ixodidae) in imported specimens from Varanus salvator (Squamata: Varanidae) in Argentina

La importación de animales vivos, especialmente reptiles, ha contribuido al transporte e introducción de garrapatas exóticas en muchas regiones del mundo. Se describe el hallazgo de 11 especímenes (un macho, dos hembras y ocho ninfas) de Amblyomma varanense colectados de dos varanos acuáticos (Varanus salvator) importados legalmente desde Jakarta, Indonesia. Amblyomma varanense es una garrapata distribuida en las regiones zoogeográficas Oriental y de Australasia, y V. salvator se encuentra entre los principales hospedadores. Es necesario prevenir la introducción de garrapatas exóticas y microrganismos asociados, poniéndose en evidencia el riesgo que conlleva la importación de vertebrados exóticos.The importation of live animals, especially reptiles, has contributed to the transport and introduction of exotic ticks in many regions of the world. We describe the finding of 11 specimens (one male, two females and eight nymphs) of Amblyomma varanense collected from two common water monitors (Varanus salvator) legally imported from Jakarta, Indonesia. Amblyomma varanense is a tick distributed in the Eastern and Australasian zoogeographic regions, and V. salvator is among the main hosts. It is necessary to prevent the introduction of exotic ticks and associated microorganisms, highlighting the risk involved in the importation of exotic vertebrates.Fil: Cicuttin, Gabriel Leonardo. Gobierno de la Ciudad Autónoma de Buenos Aires. Ministerio de Salud. Instituto de Zoonosis Luis Pasteur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wiemeyer, Guillermo. No especifíca;Fil: Pérez, M. A.. No especifíca;Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentin

Sitio web “Nombres de Especies de Garrapatas Duras”

Con el fin de ofrecer a la comunidad científica las descripciones originales de especies de garrapatas duras (Acari: Ixodida: Ixodidae) se realizó un sitio web sin fin comercial y acceso irrestricto con descripciones originales de las especies (válidas e inválidas), nombres dudosos, inciertos y una selección de nomina nuda.Sociedad Argentina de Informática e Investigación Operativa (SADIO

Sitio web “Nombres de Especies de Garrapatas Duras”

Con el fin de ofrecer a la comunidad científica las descripciones originales de especies de garrapatas duras (Acari: Ixodida: Ixodidae) se realizó un sitio web sin fin comercial y acceso irrestricto con descripciones originales de las especies (válidas e inválidas), nombres dudosos, inciertos y una selección de nomina nuda.Sociedad Argentina de Informática e Investigación Operativa (SADIO

Sitio web “Nombres de Especies de Garrapatas Duras”

Con el fin de ofrecer a la comunidad científica las descripciones originales de especies de garrapatas duras (Acari: Ixodida: Ixodidae) se realizó un sitio web sin fin comercial y acceso irrestricto con descripciones originales de las especies (válidas e inválidas), nombres dudosos, inciertos y una selección de nomina nuda.Sociedad Argentina de Informática e Investigación Operativa (SADIO

Species occurrence of ticks in South America, and interactions with biotic and abiotic traits

The datasets of records of the distribution of ticks and their hosts are invaluable tools to understand the phylogenetic patterns of evolution of ticks and the abiotic traits to which they are associated. Such datasets require an exhaustive collection of bibliographical references. In most cases, it is necessary the confirmation of reliable identification of ticks, together with an update of the scientific names of the vertebrate hosts. These data are not easily available, because many records were published in the so-called “grey literature”. Herein, we introduced the Dataset of Ticks in South America, a repository that collates data on 4,764 records of ticks (4,124 geo-referenced) with a special reference to an extra 2,370 records of ticks on cattle, together with a set of abiotic traits, curated from satellite-derived information over the complete target region. The dataset includes details of the phylogenetic relationships of the species of hosts, providing researchers with both biotic and abiotic traits that drive the distribution and evolution of ticks in South America.publishersversionpublishe

Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation

Background: Amblyomma cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Results: Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. cajennense. The phylogenetic analyses consistently found A. cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Conclusions: Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.Fil: Beati, Lorenza. Georgia State University; Estados UnidosFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria, Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Burkman, Erica J.. Georgia State University; Estados UnidosFil: Barros Battesti, Darcy M.. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil;Fil: Labruna, Marcelo B.. Universidade do Sao Paulo; Brasil;Fil: Guglielmone, Alberto Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria, Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Cáceres, Abraham G.. Instituto Nacional de Salud; PerúFil: Guzman Cornejo, Carmen. Universidad Nacional Autónoma de México; México;Fil: Léon, Renato. Universidad San Francisco de Quito; Ecuador;Fil: Durden, Lance A.. Georgia State University; Estados UnidosFil: Faccini, João L.H.. Universidade Federal Rural Do Rio de Janeiro; Brasi

Sitio web “Nombres de Especies de Garrapatas Duras”

Resumen Con el fin de ofrecer a la comunidad científica las descripciones originales de especies de garrapatas duras (Acari: Ixodida: Ixodidae) se realizó un sitio web sin fin comercial y acceso irrestricto con descripciones originales de las especies (válidas e inválidas), nombres dudosos, inciertos y una selección de nomina nuda