Development Of Hydraulic And Soil Properties For Soil Amendments And Native Soils For Retention Ponds In Marion County, Florida

The vadose zone plays an important role in managing stormwater. Predicting the water balance and water movement is crucial in ground water remediation to keep water suitable for use. To aid in understanding soils ability to transmit and store water, soil and hydraulic properties were analyzed for soils in Marion County, Florida, and potential soil amendments. Soil and hydraulic properties were examined for two soil amendments and for the soils in Marion County, Florida, at the South Oak and the Hunter\u27s Trace locations. The hydraulic properties measured were the soil moisture retention curve (SMRC) and saturated hydraulic conductivity (Ks). The soil properties measured were the particle-size distribution (PSD) and the specific gravity. From these, the bulk density and porosity were calculated. The SMRC corresponds to the water holding capacities, while the Ks corresponds to the soils ability to transmit water. Both are dependent on the soil properties. The SMRC for the soil amendments and native soils were developed in the laboratory using a Tempe Cell apparatus. In addition, the SMRC was measured in the field at the Hunter\u27s Trace location with time domain reflectometry (TDR) and tensiometer equipment at three depths of 1-ft, 2-ft, and 3-ft over approximate a two month period. The SMRC obtained in the laboratory was compared to two analytical models, Brooks and Corey and van Genuchten, and to the field data. There is a strong correlation between the laboratory, analytical, and field SMRC for both South Oak and Hunter\u27s Trace. In addition, there is a strong correlation between the laboratory SMRC and analytical models for the soil amendments. The Arya and Paris (AP) model, a pedotransfer function, was examined for its accuracy in predicting the SMRC for the soils at South Oak and Hunter\u27s Trace, in addition to the soil amendments. Measuring the SMRC in the lab is a time consuming process; therefore, inferring the SMRC from textural and structural soil properties which are easier measured characteristics would be advantageous

Collaborative learning in a high technology start-up business

This study investigated the role of collaborative learning in a new hightechnology business. It included a description of the experience of the facilitator. who was also a co-founder and officer of the company. Peters\u27 (1998, 2002) action research model was used to frame the research. Data were collected using phenomenological interviews, semi-structured interviews, and field notes. A thematic analysis revealed four themes: relationship dynamics, knowing, the facilitator\u27s role, and business outcomes. Four sub-themes appeared within the theme of relationship dynamics: team composition, early loss and change in team member participation, commitment to members, and virtual and face-to-face communication. Two sub-themes appeared within the theme of knowing: intuition and experience. These results indicated that company founders and other team members successfully engaged in collaborative learning as described by Peters and Armstrong ( 1998). Participants were able to jointly develop a dialogical space conducive to collaborative learning, practice cycles of action and reflection, utilize multiple ways of knowing, and achieve pre-start-up and start-up business outcomes such as completing incorporation documents, negotiating a technology transfer license agreement, and pursuing funding. Results also informed the way in which the researcher performed facilitator tasks within her role as company co-founder and officer. Implications for practitioners in similar start-up companies and recommendations for additional action research on collaborative learning in business contexts are discussed

Functional differentiation of midbrain neurons from human cord blood-derived induced pluripotent stem cells

INTRODUCTION: Human induced pluripotent stem cells (hiPSCs) offer great promise for regenerative therapies or in vitro modelling of neurodegenerative disorders like Parkinson’s disease. Currently, widely used cell sources for the generation of hiPSCs are somatic cells obtained from aged individuals. However, a critical issue concerning the potential clinical use of these iPSCs is mutations that accumulate over lifetime and are transferred onto iPSCs during reprogramming which may influence the functionality of cells differentiated from them. The aim of our study was to establish a differentiation strategy to efficiently generate neurons including dopaminergic cells from human cord blood-derived iPSCs (hCBiPSCs) as a juvenescent cell source and prove their functional maturation in vitro. METHODS: The differentiation of hCBiPSCs was initiated by inhibition of transforming growth factor-β and bone morphogenetic protein signaling using the small molecules dorsomorphin and SB 431542 before final maturation was carried out. hCBiPSCs and differentiated neurons were characterized by immunocytochemistry and quantitative real time-polymerase chain reaction. Since functional investigations of hCBiPSC-derived neurons are indispensable prior to clinical applications, we performed detailed analysis of essential ion channel properties using whole-cell patch-clamp recordings and calcium imaging. RESULTS: A Sox1 and Pax6 positive neuronal progenitor cell population was efficiently induced from hCBiPSCs using a newly established differentiation protocol. Neuronal progenitor cells could be further maturated into dopaminergic neurons expressing tyrosine hydroxylase, the dopamine transporter and engrailed 1. Differentiated hCBiPSCs exhibited voltage-gated ion currents, were able to fire action potentials and displayed synaptic activity indicating synapse formation. Application of the neurotransmitters GABA, glutamate and acetylcholine induced depolarizing calcium signal changes in neuronal cells providing evidence for the excitatory effects of these ligand-gated ion channels during maturation in vitro. CONCLUSIONS: This study demonstrates for the first time that hCBiPSCs can be used as a juvenescent cell source to generate a large number of functional neurons including dopaminergic cells which may serve for the development of novel regenerative treatment strategies

Image informatics strategies for deciphering neuronal network connectivity

Brain function relies on an intricate network of highly dynamic neuronal connections that rewires dramatically under the impulse of various external cues and pathological conditions. Among the neuronal structures that show morphologi- cal plasticity are neurites, synapses, dendritic spines and even nuclei. This structural remodelling is directly connected with functional changes such as intercellular com- munication and the associated calcium-bursting behaviour. In vitro cultured neu- ronal networks are valuable models for studying these morpho-functional changes. Owing to the automation and standardisation of both image acquisition and image analysis, it has become possible to extract statistically relevant readout from such networks. Here, we focus on the current state-of-the-art in image informatics that enables quantitative microscopic interrogation of neuronal networks. We describe the major correlates of neuronal connectivity and present workflows for analysing them. Finally, we provide an outlook on the challenges that remain to be addressed, and discuss how imaging algorithms can be extended beyond in vitro imaging studies

Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization

Chorea-acanthocytosis (ChAc) is a fatal neurological disorder characterized by red blood cell acanthocytes and striatal neurodegeneration. Recently, severe cell membrane disturbances based on depolymerized cortical actin and an elevated Lyn kinase activity in erythrocytes from ChAc patients were identified. How this contributes to the mechanism of neurodegeneration is still unknown. To gain insight into the pathophysiology, we established a ChAc patient-derived induced pluripotent stem cell model and an efficient differentiation protocol providing a large population of human striatal medium spiny neurons (MSNs), the main target of neurodegeneration in ChAc. Patient-derived MSNs displayed enhanced neurite outgrowth and ramification, whereas synaptic density was similar to controls. Electrophysiological analysis revealed a pathologically elevated synaptic activity in ChAc MSNs. Treatment with the F-actin stabilizer phallacidin or the Src kinase inhibitor PP2 resulted in the significant reduction of disinhibited synaptic currents to healthy control levels, suggesting a Src kinase- and actin-dependent mechanism. This was underlined by increased G/F-actin ratios and elevated Lyn kinase activity in patient-derived MSNs. These data indicate that F-actin stabilization and Src kinase inhibition represent potential therapeutic targets in ChAc that may restore neuronal function

Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation

Amyotrophic lateral sclerosis (ALS) is the most frequent motor neuron disease. Cytoplasmic fused in sarcoma (FUS) aggregates are pathological hallmarks of FUS-ALS. Proper shuttling between the nucleus and cytoplasm is essential for physiological cell function. However, the initial event in the pathophysiology of FUS-ALS remains enigmatic. Using human induced pluripotent stem cell (hiPSCs)-derived motor neurons (MNs), we show that impairment of poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response (DDR) signaling due to mutations in the FUS nuclear localization sequence (NLS) induces additional cytoplasmic FUS mislocalization which in turn results in neurodegeneration and FUS aggregate formation. Our work suggests that a key pathophysiologic event in ALS is upstream of aggregate formation. Targeting DDR signaling could lead to novel therapeutic routes for ameliorating ALS

Material Characterization And Reaction Kinetics Of Green Sorption Media For Nutrients Removal

In water scarce areas throughout the world, stormwater and wastewater used as an alternative water supply have been put into practice to promote the sustainability of our water infrastructure. Also, in water rich areas, stormwater management and on-site wastewater treatment systems are highly susceptible to extremes in precipitation causing wide spread pollution problems. High nitrogen and phosphorus content in stormwater and wastewater effluents has impeded reuse potential and impacted ecosystem integrity and human health. Nitrate may be toxic and can cause human health problem such as methemoglobinemia, liver damage and even cancers. Phosphorus may trigger the eutrophication issues in fresh water bodies, which could result in toxic algae and endanger the source of drinking waters eventually. It aims to conduct the material characterization and examine the reaction kinetics of a selected recipe of green sorption media recycled from waste streams, such as sawdust and tire crumb, combined with sand/silt and limestone, for nutrient removal using packed bed column tests as a means. Pollutants of concern include ammonia, nitrate and ortho- phosphate. Application potential in stormwater management and wastewater treatment facilities was discussed in the context of sustainable systems engineering. © 2008 ASCE