13 research outputs found
Cytotoxicity analyses of filling materials for primary teeth
Orientador: Fernanda Miori PasconDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: Endodontia em dentes decĂduos Ă© um procedimento de suma importância para manter a integridade e saĂşde dos dentes e tecidos de suporte. Sendo assim, o uso de materiais obturadores de canais radiculares que apresente o máximo de propriedades desejáveis Ă© indispensável. O objetivo do presente estudo foi analisar a citotoxicidade de diferentes materiais obturadores em fibroblastos do ligamento periodontal humano (PDL) e em cĂ©lulas osteoblásticas de osteossarcoma humano (Saos-2). Os fibroblastos foram cultivados em meio de cultura (meio Eagle modificado por Dulbeco - DMEM) suplementado com 1% soro fetal bovino (FBS) e antibiĂłticos. Os osteoblastos foram cultivados em meio de cultura McCoyÂżs suplementado com 15% FBS e antibiĂłticos. PrĂłximos de atingir a confluĂŞncia, as cĂ©lulas foram plaqueadas na concentração de 7x103 cĂ©lulas por poço e foram expostas aos seguintes materiais, conforme os grupos: G1- Meio de cultura sem material obturador (controle negativo), G2- DimetiilsulfĂłxido (DMSO) (controle positivo), G3- Calen®, G4- Calen® associada ao Ă“xido de Zinco, G5- Calen® associada ao IodofĂłrmio, G6- Ă“xido de zinco e eugenol (OZE), G7- Vitapex® e G8- UltraCal®XS. A manipulação dos materiais foi realizada em condições assĂ©pticas. A citotoxicidade dos materiais obturadores foi analisada em intervalos de tempos (8, 24 e 48 horas) pelo mĂ©todo de redução MTS e classificado como nĂŁo citotĂłxico, citotoxicidade leve, moderada e grave. O grupo controle negativo foi composto apenas por cĂ©lulas, sem o uso de material obturador. A análise morfolĂłgica das cĂ©lulas foi realizada por meio da microscopia de fluorescĂŞncia. Os dados obtidos foram submetidos Ă análise de variância dois fatores e ao teste Tukey para comparação entre os grupos, com nĂvel de significância 5%. As imagens obtidas por meio da microscopia de fluorescĂŞncia foram analisadas de forma descritiva. Os resultados mostraram que para os fibroblastos, Calen®(85,91±10,01), Calen® associada ao Ă“xido de Zinco (85,91±8,16) e Calen® associada ao IodofĂłrmio (83,96±13,95) diferiram do controle negativo (100±0) e positivo (19.72±5,70) apĂłs 8 horas de exposição. Para os osteoblastos, Calen® associada ao Ă“xido de Zinco (75,87±19,16), Calen® associada ao IodofĂłrmio (75,5±12,40) e o OZE (68,71±22,19) foram os Ăşnicos grupos que em 8 horas diferiram do controle negativo (100±0) e positivo (22,18±6,77). Pode-se concluir que todos os materiais avaliados, para fibroblastos do ligamento periodontal humano, nĂŁo foram citotĂłxicos ao longo do tempo. No entanto, Calen® associada ao IodofĂłrmio apresentou toxicidade leve em 48 horas para os osteoblastos. Vitapex® foi o material que apresentou menor toxicidade celular nos osteoblastos em 8 e 48 horas, comparando-se os outros materiais avaliados. Calen® associado ao Ă“xido de zinco, Calen® associado ao IodofĂłrmio e OZE foram capazes de modificar a morfologia dos fibroblastos, mas para os osteoblastos nĂŁo foram observadas alterações morfolĂłgicasAbstract: Endodontics in primary teeth is an important procedure to maintain the integrity and health of the teeth and supporting tissues. Using a root canal filling material that shows desirable properties is indispensable. The aim of the present study was to evaluate the root filling materials cytotoxicity on periodontal ligament fibroblasts (PDL) and osteoblastic human osteosarcoma cells (Saos-2). Fibroblasts were cultured in culture medium (Dulbeco modified Eagle medium - DMEM) supplemented with 1% fetal bovine serum (FBS) and antibiotics. The osteoblasts were cultured in McCoy's culture medium supplemented with 15% FBS and antibiotics. Next to reach confluence, the cells were plated at a concentration of 7x103 cells per well and were exposed to materials, according to the groups: G1 - culture medium without filling material (negative control); G2- Dimethyl sulfoxide (DMSO) (positive control); G3 Âż Calen®; G4 - Calen® associated with Zinc Oxide; G5 - Calen® associated with Iodoform; G6 Âż Zinc Oxide and eugenol (ZOE); G7 - Vitapex®; G8 - UltraCal® XS. The materials were prepared under aseptic conditions. Cytotoxicity was evaluated by cell viability at time intervals (8, 24 and 48 h) by MTS assay and rated as non-cytotoxic, mild, moderate and severe cytotoxicity. The negative control group was composed only of cells without the use of filling material. Cells morphological were observed by fluorescence microscopy. Data were submitted to two-way analysis of variance with post-hoc comparisons base on Tukey's multiple comparisons, with the significance level fixed at 5%. The images obtained at fluorescence microscopy were evaluated using descriptive analysis. The results showed that for fibroblasts, Calen®(85.91±10.01), Calen® associated with Zinc Oxide (85.91±8.16) e Calen® associated with Iodoform (83.96±13.95) was differ from the negative control (100±0) and positive (19.72±5.70), at 8 h. For osteoblasts, Calen® associated with Zinc Oxide (75.87±19.16), Calen® associated with Iodoform (75.5±12.40) and ZOE (68.71±22.19) differed from negative control (100±0) and positive (22.18±6.77) in 8 h. It can be concluded that all materials were non- cytotoxic to human fibroblasts cells over time. However, Calen® + Iodoform showed higher cytotoxicity to osteoblasts at 48 h. Vitapex® was the material that showed the less cell cytotoxicity in osteoblasts at 8 and 48 h, compared to the other materials tested. Calen® associated with Zinc Oxide, Calen® associated with Iodoform and ZOE was able to modify the morphology of fibroblasts, but osteoblasts but no morphologic alterations were observedMestradoOdontopediatriaMestra em Odontologi
A Web-based Platform for Quality Management of Elderly Care: Usability Evaluation of Ankira®
AbstractThe use of web-based platform for data storage and data analysis in the context quality for elderly care is improving. This work reports the usability evaluation of Ankira® Platform based in a comprehensive methodology of testing in real contexts. The participants were recruited from elderly care institutions and completed different pre-established tasks. Observation, Critical Incident Records and Scales were used to collect data. The results shown a high level of usability for the platform and indicators of reliability of the methodology are discussed
Chromosome 1p36 deletion syndrome: a report on 4 cases
Chromosome 1p36 deletion syndrome (MIM #607872) was first described in 1997 by Shapira et al. This condition is compatible with a monosomy of the 1p36 band in the distal region of the short arm of chromosome 1 and is the most common terminal deletion in humans, with an estimated prevalence of approximately 1 in 5,000 live births. This constitutional deletion is associated with mental retardation, developmental delay, seizures, hypotonia and heart defects. The syndrome is also characterized by several distinct dysmorphic features, including large anterior fontanels, microcephaly, brachycephaly, deep-set eyes, flat nose and nasal bridge, and pointed chin. The 1p36 band is not very clearly visible using classical cytogenetics, and it is therefore difficult to detect these deletions in banded karyotypes. Fluorescence in situ hybridization (FISH) and multiplex ligation-dependent probe amplification (MLPA) analysis have increasingly been used, in addition to classical cytogenetic analysis, in children with mental retardation in order to identify this chromosomal abnormality. The authors present four patients between 1 month and 14 years of age with apparently normal karyotypes. Using molecular cytogenetic techniques, all cases showed a “pure” 1p36 deletion: three were detected by FISH (CEB108/T7, located at 1p36.3, Vysis) and are “de novo”; the fourth was detected by MLPA (P036 and P070, MRC Holland) analysis, and its origin is still unknown. The phenotypes of these patients are described and compared with other cases having this syndrome, described in the literature. We also emphasize the importance of good clinical characterization in order to establish the best cytogenetic strategy to assure accurate diagnosis
Meanings and practices of oral health: a qualitative study with mothers of disabled children
The aim of this study was to understand the meanings and oral health practices of ten mothers of disabled children between the ages of zero and three years. The qualitative method was used. The data was collected by means of a semi-structured interview and were recorded, transcribed and analysed using the Thematic Content Analysis. Regarding to the oral health care of the mothers, three reported difficult and traumatising experiences and three did not have much contact with dentists during their childhood. Regarding to the children, the age of their first visit to the dentist varied from three months to two years and six months, and the reason for this appointment for seven children was referral by their doctor or by the Association of Parents and Friends of the Exceptional. Seven mothers reported that they brushed the teeth of their children at least twice a day and only two cited the importance of regular dental appointments. For two mothers, the meaning of oral health was to have a perfect smile and for four others, maintain oral hygiene. Half of the mothers related oral health to general health. All reported that dental caries is related to diet and lack of care regarding to oral hygiene. It was possible to conclude that for this group of mothers, oral health has an important meaning and that oral health practices are based on a healthy diet and brushing. Although mothers of disabled children have presented positive attitudes, in general, the results indicated limited knowledge about oral health. The results showed the importance of considering the point of view of the mothers as data of analysis bringing greater proximity with the reality studied. More qualitative studies should be conducted in order to collaborate with the improvement of the oral condition of disabled children
Genomic Characterization of mcr-1.1-Producing Escherichia coli Recovered From Human Infections in SĂŁo Paulo, Brazil
Polymyxins are one of most important antibiotics available for multidrug-resistant Gram-negative infections. Diverse chromosomal resistance mechanisms have been described, but the polymyxin resistance phenotype is not yet completely understood. The objective of this study was to characterize colistin resistant mcr-1-producing strains isolated from human infections over one year in a hospital setting (Hospital das ClĂnicas, SĂŁo Paulo, Brazil). We isolated 490 colistin-resistant Gram-negative rods, of which eight were mcr-1.1-positive Escherichia coli, the only species with this result, indicating a low incidence of the mcr-1 production mechanism among colistin-resistant isolates. All mcr-1.1 positive isolates showed similarly low MICs for colistin and were susceptible to most antibiotics tested. The isolates showed diversity of MLST classification. The eight mcr-1.1-positive E. coli genomes were sequenced. In seven of eight isolates the mcr-1.1 gene is located in a contig that is presumed to be a part of an IncX4 plasmid; in one isolate, it is located in a contig that is presumed to be part of an IncHI2A plasmid. Three different genomic contexts for mcr-1.1 were observed, including a genomic cassette mcr-1.1-pap2 disrupting a DUF2806 domain-containing gene in six isolates. In addition, an IS1-family transposase was found inserted next to the mcr-1.1 cassette in one isolate. An mcr-1.1-pap2 genomic cassette not disrupting any gene was identified in another isolate. Our results suggest that plasmid dissemination of hospital-resident strains took place during the study period and highlight the need for continued genomic surveillance
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4
While the increasing availability of global databases on ecological communities has advanced our knowledge
of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In
the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of
Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus
crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced
environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian
Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by
2050. This means that unless we take immediate action, we will not be able to establish their current status,
much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost
Alterações no perfil facial decorrentes do tratamento ortodôntico
Atualmente, verifica-se um aumento expressivo de pacientes que
procuram tratamentos ortodĂ´nticos. Esse crescimento deve-se pela busca
incansável por melhora da estética e não por apenas alterações das funções e as
conseqüências desta. Os jovens são a maioria destes pacientes, daà a importância
de executar um correto diagnĂłstico e conseqĂĽentemente planejamento do caso, pois
estes passam por mudanças no perfil facial e tecido tegumentar, e qualquer
alteração mal planejada pode influenciar no seu perfil e conseqüentemente no bem
estar psĂquico e emocional. O presente trabalho realizou uma revisĂŁo de literatura
abordando as principais alterações faciais decorrentes do crescimento e do
tratamento ortodôntico e se existe dimorfismo sexual. Para verificar essas alterações
faciais, os autores, realizaram traçados cefalométricos com o objetivo de analisar o
tecido dento esquelético e o tecido tegumentar. Nesta revisão de literatura foi
possĂvel concluir a importância de um correto diagnĂłstico e planejamento para obter
sucesso ao final do tratamento ortodĂ´ntico.Currently, there is a significant increase of patients for orthodontic
treatment. This growth is due to the relentless search for improved aesthetics and not
just for a change of functions and the consequences of this. Young people are the
majority of these patients, hence the importance of performing a correct diagnosis
and consequently case planning, because they go through changes in facial profile
and soft tissue, and any amendment poorly planned can influence in your profile and
consequently in the psychic and emotional well being. This work made a literature
review addressing the major changes resulting from facial growth and orthodontic
treatment and if there is sexual dimorphism. To check these facial changes, the
authors performed cephalometric tracings in order to analyze the skeletal dental
tissue and soft tissue. This literature review was possible to conclude the importance
of a correct diagnosis and planning for success at the end of orthodontic treatment
Familial manifestation of hereditary hemorrhagic telangiectasia
Segundo a Organização Mundial de SaĂşde, as doenças sĂŁo consideradas raras quando afetam atĂ© 65 pessoas em cada 100.000 indivĂduos, ou seja, 1,3 para cada 2.000 pessoas. A SĂndrome de Osler-Weber-Rendu ou tambĂ©m conhecida como Telangiectasia Hemorrágica Hereditária, Ă© uma das doenças raras descritas na literatura, de herança autossĂ´mica dominante, que acomete o tecido fibrovascular e afeta um a cada 5000/10000 pessoas. É caracterizada pela trĂade: epistaxes recorrentes, telangiectasias em pele e mucosas e malformações arteriovenosas, alĂ©m do padrĂŁo familiar. O diagnĂłstico Ă© baseado nos critĂ©rios de Curação e confirmado na presença de no mĂnimo trĂŞs das manifestações descritas anteriormente. O tratamento Ă© realizado de acordo com o tipo de manifestação apresentada. Este estudo descreve o padrĂŁo familiar da Telangiectasia Hemorrágica Hereditária e suas manifestações clĂnicas no caso de uma famĂlia com o pai de 60 anos de idade e duas filhas, com SĂndrome de Rendu-Osler-WeberAccording to the World Health Organization, the disease is considered rare when affect up to 65 people in every 100,000 individuals, i.e. 1.3 for every 2000 people. Osler-Weber-Rendu Syndrome or also known as Hereditary hemorrhagic Telangiectasia, is a rare disease described in the literature, of autosomal dominant inheritance, that affects the fibrovascular tissue and affects 1 every 5000/10000 people. It is characterized by the triad: epistaxes applicants, Telangiectasia on mucous membranes and skin and arteriovenous malformations; In addition to the familiar pattern. The diagnosis is based on the criteria of Curaçao and confirmed in the presence of at least three of the demonstrations described previously. The treatment is performed according to the type of expression. This study describes the familiar pattern of hereditary hemorrhagic Telangiectasia and its clinical manifestations in the case of a family with the 60-year-old father and two daughters with Rendu-Osler-Weber Syndrom