Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana

How stable and temperature-dependent variations in DNA methylation and nucleosome occupancy influence alternative splicing (AS) remains poorly understood in plants. To answer this, we generated transcriptome, whole-genome bisulfite, and MNase sequencing data for an epigenetic Recombinant Inbred Line (epiRIL) of A. thaliana at normal and cold temperature. For comparative analysis, the same data sets for the parental ecotype Columbia (Col-0) were also generated, whereas for DNA methylation, previously published high confidence methylation profiles of Col-0 were used. Significant epigenetic differences in an identical genetic background were observed between Col-0 and epiRIL lines under normal and cold temperatures. Our transcriptome data revealed that differential DNA methylation and nucleosome occupancy modulate expression levels of many genes and AS in response to cold. Collectively, DNA methylation and nucleosome levels exhibit characteristic patterns around intron-exon boundaries at normal and cold conditions, and any perturbation in them, in an identical genetic background is sufficient to modulate AS in Arabidopsis

Portable UWB RADAR Sensing System for Transforming Subtle Chest Movement into Actionable Micro-Doppler Signatures to Extract Respiratory Rate Exploiting ResNet Algorithm

Contactless or non-invasive technology for the monitoring of anomalies in an inconspicuous and distant environment has immense significance in health-related applications, in particular COVID-19 symptoms detection, diagnosis, and monitoring. Contactless methods are crucial specifically during the COVID-19 epidemic as they require the least amount of involvement from infected individuals as well as healthcare personnel. According to recent medical research studies regarding coronavirus, individuals infected with novel COVID-19-Delta variant undergo elevated respiratory rates due to extensive infection in the lungs. This appalling situation demands constant real-time monitoring of respiratory patterns, which can help in avoiding any pernicious circumstances. In this paper, an Ultra-Wideband RADAR sensor “XeThru X4M200” is exploited to capture vital respiratory patterns. In the low and high frequency band, X4M200 operates within the 6.0-8.5 GHz and 7.25-10.20 GHz band, respectively. The experimentation is conducted on six distinct individuals to replicate a realistic scenario of irregular respiratory rates. The data is obtained in the form of spectrograms by carrying out normal (eupnea) and abnormal (tachypnea) respiratory. The collected spectrogram data is trained, validated, and tested using a cutting-edge deep learning technique called Residual Neural Network or ResNet. The trained ResNet model’s performance is assessed using the confusion matrix, precision, recall, F1-score, and classification accuracy. The unordinary skip connection process of the deep ResNet algorithm significantly reduces the underfitting and overfitting problem, resulting in a classification accuracy rate of up to 90%

PROCESS MODELS DISCOVERY AND TRACES CLASSIFICATION: A FUZZY-BPMN MINING APPROACH.

The discovery of useful or worthwhile process models must be performed with due regards to the transformation that needs to be achieved. The blend of the data representations (i.e data mining) and process modelling methods, often allied to the field of Process Mining (PM), has proven to be effective in the process analysis of the event logs readily available in many organisations information systems. Moreover, the Process Discovery has been lately seen as the most important and most visible intellectual challenge related to the process mining. The method involves automatic construction of process models from event logs about any domain process, and describes causal dependencies between the various activities as performed within the process execution environment. In principle, one can use process discovery to obtain process models that describes reality. To this end, the work in this artcle presents a Fuzzy-BPMN mining approach that uses training events log representing 10 different real-time business process executions to provide a method for discovery of useful process models, and then cross-validating the derived models with a set of test event logs in order to measure the accuracy and performance of the employed approach. The method focuses on carrying out a classification task to determine the traces, i.e. individual cases that makes up the test event logs in order to determine which traces that can be replayed by the original model. Thus, the paper aim is to provide a technique for process models discovery which is as good in balancing between “overfitting” and “underfitting” as it is able to correctly classify the traces that can be replayed (i.e allowed) or non-replayable (disallowed) by the model. In other words, the study shows through the Fuzzy-BPMN replaying notation and the series of validation experiments - how given any classified trace (for the test events log) and discovered process model (the training log) it can be unambiguously determined whether or not the traces found can be replayed on the discovered model

GENETIC DIVERSITY OF FABA BEAN (VICIA FABA L.) POPULATIONS ESTIMATED BY ISOZYMIC AND MOLECULAR MARKERS: RELATIONSHIP BETWEEN THE TWO METHODS

In our previous studies, the genetic diversity among nine Tunisian faba bean (Vicia faba) populations was analysed using isozymes and sequence-specific amplification polymorphism (SSAP) markers. The objectives of this study were to compare the application and utility of isozymes and sequence-specific amplification polymorphism (SSAP) techniques for analysis of genetic diversity among nine Tunisian faba bean (Vicia faba) populations. A high genetic diversity within populations was detected by both isozymes (SOD, 6-PGD, ME, EST, SKDH, FDH and GDH) and (SSAP) markers (PDR1, Tps19 and Tvf4). For all populations, the genetic diversity revealed by SSAP was more pronounced than that detected with isozyme, based on polymorphic profiles. The analyses of correspondance between the tow methodes based in Mantel test revealed a low correlation ( r=0.177). The low correspondance indicated the absence of correlation and therfore the complimentarit

Discrete Human Activity Recognition and Fall Detection by Combining FMCW RADAR Data of Heterogeneous Environments for Independent Assistive Living

Human activity monitoring is essential for a variety of applications in many fields, particularly healthcare. The goal of this research work is to develop a system that can effectively detect fall/collapse and classify other discrete daily living activities such as sitting, standing, walking, drinking, and bending. For this paper, a publicly accessible dataset is employed, which is captured at various geographical locations using a 5.8 GHz Frequency-Modulated Continuous-Wave (FMCW) RADAR. A total of ninety-nine participants, including young and elderly individuals, took part in the experimental campaign. During data acquisition, each aforementioned activity was recorded for 5–10 s. Through the obtained data, we generated the micro-doppler signatures using short-time Fourier transform by exploiting MATLAB tools. Subsequently, the micro-doppler signatures are validated, trained, and tested using a state-of-the-art deep learning algorithm called Residual Neural Network or ResNet. The ResNet classifier is developed in Python, which is utilised to classify six distinct human activities in this study. Furthermore, the metrics used to analyse the trained model’s performance are precision, recall, F1-score, classification accuracy, and confusion matrix. To test the resilience of the proposed method, two separate experiments are carried out. The trained ResNet models are put to the test by subject-independent scenarios and unseen data of the above-mentioned human activities at diverse geographical spaces. The experimental results showed that ResNet detected the falling and rest of the daily living human activities with decent accuracy

Alternative Splicing and Protein Diversity: Plants Versus Animals

Plants, unlike animals, exhibit a very high degree of plasticity in their growth and development and employ diverse strategies to cope with the variations during diurnal cycles and stressful conditions. Plants and animals, despite their remarkable morphological and physiological differences, share many basic cellular processes and regulatory mechanisms. Alternative splicing (AS) is one such gene regulatory mechanism that modulates gene expression in multiple ways. It is now well established that AS is prevalent in all multicellular eukaryotes including plants and humans. Emerging evidence indicates that in plants, as in animals, transcription and splicing are coupled. Here, we reviewed recent evidence in support of co-transcriptional splicing in plants and highlighted similarities and differences between plants and humans. An unsettled question in the field of AS is the extent to which splice isoforms contribute to protein diversity. To take a critical look at this question, we presented a comprehensive summary of the current status of research in this area in both plants and humans, discussed limitations with the currently used approaches and suggested improvements to current methods and alternative approaches. We end with a discussion on the potential role of epigenetic modifications and chromatin state in splicing memory in plants primed with stresses

Multiple Participants’ Discrete Activity Recognition in a Well-Controlled Environment Using Universal Software Radio Peripheral Wireless Sensing

Wireless sensing is the utmost cutting-edge way of monitoring different health-related activities and, concurrently, preserving most of the privacy of individuals. To meet future needs, multi-subject activity monitoring is in demand, whether it is for smart care centres or homes. In this paper, a smart monitoring system for different human activities is proposed based on radio-frequency sensing integrated with ensemble machine learning models. The ensemble technique can recognise a wide range of activity based on alterations in the wireless signal’s Channel State Information (CSI). The proposed system operates at 3.75 GHz, and up to four subjects participated in the experimental study in order to acquire data on sixteen distinct daily living activities: sitting, standing, and walking. The proposed methodology merges subject count and performed activities, resulting in occupancy count and activity performed being recognised at the same time. To capture alterations owing to concurrent multi-subject motions, the CSI amplitudes collected from 51 subcarriers of the wireless signals were processed and merged. To distinguish multi-subject activity, a machine learning model based on an ensemble learning technique was designed and trained using the acquired CSI data. For maximum activity classes, the proposed approach attained a high average accuracy of up to 98%. The presented system has the ability to fulfil prospective health activity monitoring demands and is a viable solution towards well-being tracking

Software-defined radio based contactless localization for diverse human activity recognition

This paper presents a study on contactless localization for activity recognition based on radio-frequency sensing. The focus of this study is on the quantitative analysis of the collected data, which is in the form of channel state information (CSI). The proposed method utilizes a software-defined radio (SDR) system in combination with an ensemble learning technique to localize and identify daily living activities such as leaning, sitting, standing and walking. Specifically, SDR device, Universal Software Radio Peripheral (USRP) models X300/X310 are utilized to collect data on the aforementioned activities. The data is collected from an empty space and a participant performing daily living activities in different territories. The acquired data is labelled based on the region, zone and performed activity. The CSI data is subsequently preprocessed and fed into an ensemble-based machine learning algorithm for classification. Furthermore, the stability analysis of the proposed method is performed to evaluate its reliability and robustness. The performance of the algorithm is evaluated using various metrics, including a confusion matrix, accuracy, cross-validation score and training time. The results demonstrate that the proposed ensemble-based approach achieves a high accuracy of up to 90% in activity recognition, highlighting the effectiveness of the proposed method in contactless localization for activity recognition

Contactless Small-Scale Movement Monitoring System Using Software Defined Radio for Early Diagnosis of COVID-19

The exponential growth of the novel coronavirus disease (N-COVID-19) has affected millions of people already and it is obvious that this crisis is global. This situation has enforced scientific researchers to gather their efforts to contain the virus. In this pandemic situation, health monitoring and human movements are getting significant consideration in the field of healthcare and as a result, it has emerged as a key area of interest in recent times. This requires a contactless sensing platform for detection of COVID-19 symptoms along with containment of virus spread by limiting and monitoring human movements. In this paper, a platform is proposed for the detection of COVID-19 symptoms like irregular breathing and coughing in addition to monitoring human movements using Software Defined Radio (SDR) technology. This platform uses Channel Frequency Response (CFR) to record the minute changes in Orthogonal Frequency Division Multiplexing (OFDM) subcarriers due to any human motion over the wireless channel. In this initial research, the capabilities of the platform are analyzed by detecting hand movement, coughing, and breathing. This platform faithfully captures normal, slow, and fast breathing at a rate of 20, 10, and 28 breaths per minute respectively using different methods such as zero-cross detection, peak detection, and Fourier transformation. The results show that all three methods successfully record breathing rate. The proposed platform is portable, flexible, and has multifunctional capabilities. This platform can be exploited for other human body movements and health abnormalities by further classification using artificial intelligence

Novel Privacy Preserving Non-Invasive Sensing-Based Diagnoses of Pneumonia Disease Leveraging Deep Network Model

This article presents non-invasive sensing-based diagnoses of pneumonia disease, exploiting a deep learning model to make the technique non-invasive coupled with security preservation. Sensing and securing healthcare and medical images such as X-rays that can be used to diagnose viral diseases such as pneumonia is a challenging task for researchers. In the past few years, patients’ medical records have been shared using various wireless technologies. The wireless transmitted data are prone to attacks, resulting in the misuse of patients’ medical records. Therefore, it is important to secure medical data, which are in the form of images. The proposed work is divided into two sections: in the first section, primary data in the form of images are encrypted using the proposed technique based on chaos and convolution neural network. Furthermore, multiple chaotic maps are incorporated to create a random number generator, and the generated random sequence is used for pixel permutation and substitution. In the second part of the proposed work, a new technique for pneumonia diagnosis using deep learning, in which X-ray images are used as a dataset, is proposed. Several physiological features such as cough, fever, chest pain, flu, low energy, sweating, shaking, chills, shortness of breath, fatigue, loss of appetite, and headache and statistical features such as entropy, correlation, contrast dissimilarity, etc., are extracted from the X-ray images for the pneumonia diagnosis. Moreover, machine learning algorithms such as support vector machines, decision trees, random forests, and naive Bayes are also implemented for the proposed model and compared with the proposed CNN-based model. Furthermore, to improve the CNN-based proposed model, transfer learning and fine tuning are also incorporated. It is found that CNN performs better than other machine learning algorithms as the accuracy of the proposed work when using naive Bayes and CNN is 89% and 97%, respectively, which is also greater than the average accuracy of the existing schemes, which is 90%. Further, K-fold analysis and voting techniques are also incorporated to improve the accuracy of the proposed model. Different metrics such as entropy, correlation, contrast, and energy are used to gauge the performance of the proposed encryption technology, while precision, recall, F1 score, and support are used to evaluate the effectiveness of the proposed machine learning-based model for pneumonia diagnosis. The entropy and correlation of the proposed work are 7.999 and 0.0001, respectively, which reflects that the proposed encryption algorithm offers a higher security of the digital data. Moreover, a detailed comparison with the existing work is also made and reveals that both the proposed models work better than the existing work