Impact of single and repeated applications of the insecticide chlorpyrifos on tropical freshwater plankton communities

This paper describes the effects of a single and a repeated application of the organophosphorus insecticide chlorpyrifos on zooplankton and phytoplankton communities in outdoor microcosms in Thailand. Treatment levels of 1 mu g L-1 were applied once or twice with a 2-week interval. Both treatments led to a significant decrease in cladocerans followed by an increase in rotifers, although the extent by which species were affected was different. Ceriodaphnia cornuta was the most responding cladoceran after the first treatment, while Moina micrura responded most to the second. This is explained by differences in the growth phase of M. micrura at the time of application and an increase in Microcystis abundance over the course of the experiment. Several phytoplankton taxa either increased or decreased as a result of the chlorpyrifos-induced changes in zooplankton communities. Even though chlorpyrifos disappeared fast from the water column, effects on plankton communities persisted till the end of the experiment (42 days) when the insecticide concentrations had dropped below the detection limit. This was presumably due to the increasing population trend of Microcystis, favouring rotifers over cladocerans

Draft genome sequence of Agrobacterium deltaense strain CNPSo 3391, isolated from a soybean nodule in Mozambique

Open Access Article; Published online: 7 March 2019Agrobacterium deltaense strain CNPSo 3391 was isolated from a soybean nodule in Mozambique. Its genome size was estimated at 4,926,588 bp. This isolate carries several coding sequences for stress tolerance, but no identifiable nodulation or virulence genes. Possible ecological roles of bacteria isolated from legume nodules and closely related to Agrobacterium are discussed

Permittivity of (40 nm and 80 nm) alumina nanofluids in ethylene glycol at different temperatures

This article studies the effective permittivity of alumina nanofluids (aluminium oxide) in ethylene glycol. Two nanoparticle sizes (40 nm and 80 nm) were considered and the measurements were carried out at various concentrations (up to 2% in volume) and at six different temperatures (from 298.15 K to 348.15 K). An empirical equation is proposed that allows to obtain the permittivity value at any concentration or temperature in the studied ranges. The influence of the volume fraction, nanoparticle size and the temperature on relative permittivity is shown. When compared to the previous published values for alumina (40 nm) in water, current results show the influence of the base fluid. The enhancement of permittivity was calculated, and its behaviour was analysed. Smaller sized particles have the highest values of permittivity and enhancement. Theoretical models in the study of permittivity are applied. The poor predictions of classical models are attributed to the positive behaviour of the permittivity change on mixing for these nanofluids. The contributions to permittivity from ethylene glycol and nanoparticles are separated in two distinct terms in the variable index equation. The permittivity change on mixing calculated from this equation points out that the nanoparticles are the main responsible for the unusual permittivity increment in these colloids.We appreciate the financial support ED431C 2020-06 provided by the Xunta de Galicia (Spain). M.F.C. thanks Instituto Superior de Engenharia do Porto for granting leave of absence to carry out experimental work at University of Vigo.info:eu-repo/semantics/publishedVersio

Isolation, characterization and selection of indigenous Bradyrhizobium strains with outstanding symbiotic performance to increase soybean yields in Mozambique.

Article purchasedSoybean inoculation with effective rhizobial strains makes unnecessary the use of N-fertilizers in the tropics. A frequently reported problem is the failure of the inoculant strains to overcome the competition imposed by indigenous rhizobial populations. The screening of indigenous rhizobia, already adapted to local conditions, searching for highly effective strains for use as inoculants represents a promising strategy in overcoming inoculation failure. The objective of this study was to isolate and characterize indigenous rhizobia and to identify strains that hold potential to be included in inoculant formulations for soybean production, with both promiscuous and non-promiscuous soybean cultivars, in Mozambican agro-climatic conditions. A total of 105 isolates obtained from nodules of promiscuous soybean grown at 15 sites were screened for N2-fixation effectiveness in the greenhouse along with five commercial strains. Eighty-seven isolates confirmed the ability to form effective nodules on soybean and were used for genetic characterization by rep-PCR (BOX) and sequencing of the 16S rRNA gene, and also for symbiotic effectiveness. BOX-PCR fingerprinting revealed remarkable genetic diversity, with 41 clusters formed, considering a similarity level of 65%. The 16S rRNA analysis assigned the isolates to the genera Bradyrhizobium (75%) and Agrobacterium/Rhizobium (25%). Great variability in symbiotic effectiveness was detected among the indigenous rhizobia from Mozambique, with ten isolates performing better than the commercial strain B. diazoefficiens USDA 110, the best reference strain, and 51 isolates with lower performance than all reference strains. Thirteen of the best isolates from the first greenhouse trial were evaluated, along with the five commercial strains, in two promiscuous (TGx 1963-3F and TGx 1835-10E) and one non-promiscuous (BRS 284) soybean cultivars in a second greenhouse trial. In general the promiscous soybeans responded better to inoculation. The 13 isolates were also characterized for tolerance to acidity and alkalinity (pH 3.5 and 9.0, respectively), salinity (0.1, 0.3 and 0.5 mol L−1 of NaCl) and high temperatures (35, 40 and 45 °C) in vitro. Five isolates, three (Moz 4, Moz 19 and Moz 22) belonging to the superclade B. elkanii and two (Moz 27 and Moz 61) assigned to the superclade B. japonicum, consistently showed high symbiotic effectiveness, suggesting that the inoculation with indigenous rhizobia adapted to local conditions represents a possible strategy for increasing soybean yields in Mozambique. Phylogenetic position of the five elite isolates was confirmed by the MLSA with four protein-coding housekeeping genes, dnaK, glnII, gyrB and recA

Compositional specification of functionality and timing of manufacturing systems

In this paper, a formal modeling approach is introduced for compositional specification of both functionality and timing of manufacturing systems. Functionality aspects can be considered orthogonally to the timing. The functional aspects are specified using two abstraction levels; high-level activities and lower level actions. Design of a functionally correct controller is possible by looking only at the activity level, abstracting from the different execution orders of actions. Furthermore, the specific timing of actions is not needed. As a result, controller designcan be performed on a much smaller state space compared to an explicit model where timing and actions are present. The performance of the controller can be analyzed and optimizedby taking into account the timing characteristics. Since formal semantics are given in terms of a (max, +) state space, various existing performance analysis techniques can be used. Weillustrate the approach, including performance analysis, on an example manufacturing system

Wave function mapping conditions in Open Quantum Dots structures

We discuss the minimal conditions for wave function spectroscopy, in which resonant tunneling is the measurement tool. Two systems are addressed: resonant tunneling diodes, as a toy model, and open quantum dots. The toy model is used to analyze the crucial tunning between the necessary resolution in current-voltage characteristics and the breakdown of the wave functions probing potentials into a level splitting characteristic of double quantum wells. The present results establish a parameter region where the wavefunction spectroscopy by resonant tunneling could be achieved. In the case of open quantum dots, a breakdown of the mapping condition is related to a change into a double quantum dot structure induced by the local probing potential. The analogy between the toy model and open quantum dots show that a precise control over shape and extention of the potential probes is irrelevant for wave function mapping. Moreover, the present system is a realization of a tunable Fano system in the wave function mapping regime.Comment: 6 pages, 6 figure

O diagnóstico da síndrome do carcinoma nevóide basocelular (gorlimgoltz)

Introdução: A síndrome de Gorlin-Goltz é compreendida como uma condição hereditária autossômica dominante, causada por uma alteração no gene patched (PTCH), localizado no cromossomo 9q22.3-q31, este gene regula as funções de supressão tumoral, organização embrionária e ciclo celular, as mutações no gene PTCH levam a o efeito da perda de controle de vários outros genes responsaveis pela organogense, carcinogenese e odontogenese. Relato de caso: Paciente, feoderma, 17 anos, foi encaminhando ao serviço de Cirurgia e Traumatologia Bucomaxilofacial, com queixa de aumento de volume na região de maxila e mandibula à direta, associado a um quadros de dores. Durante a anamnese o paciente negou doenças ou comorbidades prévias. Ao exame fisico, apresentou assimetria facial, com tumefação em hemiface direita, discreto hipertelorismo, bossa frontal acentuada, multiplas maculas na região torax e membro superior direito. O exame tomografico de face evidenciavam-se multiplas imagens hipodensas em todos os quadrantes da maxila e mandibula. Com base no metodo de diagnostico e Evans, o paciente foi diagnostico com a sindrome de Gorlin-Goltz. Após o fechamento do diagnotisco optou-se pela descompressões das lesões maiores e enucleação das menores. Conclusão: O diagnostico clinico baseado nos criterios ja estabelicido por Evans em 1991, e se mostrou eficaz para definição do caso apresentado neste trabalho.Palavras-chave: Diagnostico. Sindrome do nevo basocelular. Patologia

R--Parity Violating Signals for Chargino Production at LEP II

We study chargino pair production at LEP II in supersymmetric models with spontaneously broken R-parity. We perform signal and background analyses, showing that a large region of the parameter space of these models can be probed through chargino searches at LEP II. In particular, we determine the attainable limits on the chargino mass as a function of the magnitude of the effective bilinear R-parity violation parameter $\epsilon$, demonstrating that LEP II is able to unravel the existence of charginos with masses almost up to its kinematical limit even in the case of R-parity violation. This requires the study of several final state topologies since the usual MSSM chargino signature is recovered as $\epsilon \to 0$. Moreover, for sufficiently large $\epsilon$ values, for which the chargino decay mode $\chi^\pm \to \tau^\pm J$ dominates, we find through a dedicated Monte Carlo analysis that the $\chi^\pm$ mass bounds are again very close to the kinematic limit. Our results establish the robustness of the chargino mass limit, in the sense that it is basically model-independent. They also show that LEP II can establish the existence of spontaneous R-parity violation in a large region of parameter space should charginos be produced.Comment: improved analyses; 31 pages and 9 figures (included

New Signatures for a Light Stop at LEP2 in SUSY Models with Spontaneously Broken R-Parity

In a class of supersymmetric models with R-parity breaking the lightest stop can have new decay modes into third generation fermions, $\tilde{t}_1 \rightarrow b + \tau$. We show that this decay may be dominant or at least comparable to the ordinary R-parity conserving mode $\tilde{t}_1 \rightarrow c + \tilde{\chi}_1^0$, where $\tilde{\chi}_1^0$ denotes the lightest neutralino. The new R-parity violating decay mode could provide new signatures for stop production at LEP.Comment: uudecoded latex file, 12 pages with 3 figures included. The complete uudecoded ps paper is also available via anonymous ftp at ftp://neutrinos.uv.es/pub/papers/ps/stop1.u

Dihedral-angle Gaussian distribution driving protein folding

The proposal of this paper is to provide a simple angular random walk model to build up polypeptide structures, which encompass properties of dihedral angles of folded proteins. From this model, structures will be built with lengths ranging from 125 up to 400 amino acids for the different fractions of secondary structure motifs, which dihedral angles were randomly chosen according to narrow Gaussian probability distributions. In order to measure the fractal dimension of proteins three different cases were analyzed. The first contained alpha-helix structures only, the second beta-strands structures and the third a mix of alpha-helices and beta-sheets. The behavior of proteins with alpha-helix motifs are more compacted than in other situations. The findings herein indicate that this model describes some structural properties of a protein and suggest that randomness is an essential ingredient but proteins are driven by narrow angular Gaussian probability distributions and not by random-walk processes.Comment: 9 pages, 4 figures, to be published in Physica