Rancangan Perbaikan Kualitas Pelayanan Di Pusat Pelayanan Pelanggan Pt.x Dengan Pendekatan Model Servqual Dan Intqual

Telecommunications business competition in the era of globalization increase strictly. Good service quality becomes an important key to win the competition. The visitors' feedbacks of customer service centre PT. X indicate dissatisfaction with personnel and physical aspect there. This research was conducted to identify factors that influence visitor and CSR officers satisfaction with SERVQUAL and Internal Service Quality model approach. The results obtained show that the external service quality variables consisting of tangibles, assurance, responsiveness, empathy and reliability has a correlation to customer satisfaction with value respectively 0.339, 0427, 0.542, 0.386, and 0.542. These five variables also have a significant relationship to customer satisfaction. Gap analysis results indicate that the assurance variable has the highest value gap at 1.41. The variables in the quality of internal services such as facilities / equipment, business processes and human resource development has a correlation to employee satisfaction with value respectively 0.604, 0.524 and 0.608. These three variables also had a significant relationship with employee satisfaction. Human resource development variable also has the highest gap value, ie 1.59. The design of the training with basic training, achievement motivation training, and customer service excellence materials is expected to be a solution to bridge both of the above, in order to improve the quality of services at the customer service center PT. X, giving satisfaction to customers and also employees themselves .Kata Kunci: SERVQUAL, Internal Service Quality, Correlation, Gap Analysis dan Training PendahuluanGlobalisasi membuat iklim USAha menjadi semakin kompetitif, demikian pula di bidang telekomunikasi. Kompetisi bisnis semakin ketat dengan semakin banyaknya bermunculan operator telekomunikasi di Indonesia, baik yang merupakan investasi lokal maupun investasi asing. Saat ini ada sekitar sepuluh Perusahaan telekomunikasi yang beroperasi di Indonesia (Wikipedia, telekomunikasi seluler di Indonesia). Dengan banyaknya operator telekomunikasi ini, konsumen dengan mudah berpindah-pindah operator sesuai keinginan mereka. Beberapa Perusahaan telekomunikasi juga berpendapat bahwa era untuk mendapatkan pelanggan baru melalui layanan tradisional seperti layanan suara akan sampai di titik jenuh setelah penetrasi pelanggan mencapai 80%. Titik jenuh ini menyebabkan para operator mulai menjauhi kompetisi untuk mendapatkan pelanggan baru (Tekno Jurnal, 2011). Dengan demikian, kualitas pelayanan menjadi kunci penting bagi setiap operator telekomunikasi untuk memenangkan kompetisi atau untuk bertahan dalam kompetisi yang semakin ketat. Pelayanan yang baik akan meningkatkan kepuasan konsumen, sehingga merupakan strategi paling ampuh untuk mempertahankan pelanggan

PENETAPAN HARGA JUAL IKAN SEGAR DIKOTA AMBON

One of the objectives of pricing is to achieve stable prices that do not easily rise and fall. With fixed and stable prices, we can avoid potential price wars. This price war can occur when there is a decrease in demand. Product prices are forced down in order to attract buyers. Pricing is influenced by Internal Factors and External Factors. Internal factors include the company's marketing objectives, costs, marketing mix strategies, and pricing methods. Meanwhile, external factors include the nature of the market and demand, competition, and other environmental elements. Selling price is an important thing that is of concern to producers in smoked fish marketing because it will determine the profits obtained for business continuity (Tjiptono, 2015). To achieve a profit desired by producers, one of the ways that needs to be done to attract consumer interest is to determine the selling price of the product appropriately (Waryanto and Nasrulloh, 2014). This is because the price level set will affect the quantity of products sold, because from the consumer's point of view the price is an indicator of the benefits obtained. Fish is one of the fishery products that is easily rotten and requires extra tight handling, so that the quality of the product can be maintained and the price to be offered can be adjusted to existing conditions. Fresh fish is fish that has characteristics including prominent eyes, red gills, complete scales, chewy meat, fresh aroma, and solid tail

Aging of polymeric composites : a literature review

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2013.Cataloged from PDF version of thesis.Includes bibliographical references (pages 97-103).Due to their increased use in today's society, an extensive survey was undertaken in this report to condense what's been, thus far, discovered as to the effects of aging on polymeric composites. Special emphasis was placed on composites with vinyl ester as the matrix reinforced with glass or carbon fibers. Moisture exposure, thermal conditioning, and cyclic loading were the aging mechanisms used to mimic environmental effects. Fatigue, flexural, tensile tests, and scanning electron microscopy were among the many methods used in studies to determine the effects of aging on composites. The deleterious effect of moisture on composite performance was undeniable, with the drop in flexural strength being as high as 48%. Studies found no difference between salt and distilled water aging, although salt water was reported as being more slowly absorbed. Composite materials were observed to experience no further loss of strength beyond the point of saturation. Findings showed that, after aging, composites retained some of their strength, with flexural strength retention reported as high as 66%. Frequently, the glass transition temperatures of the materials changed depending on the aging process. In most cases, Fick's law of diffusion was confirmed as an accurate model for moisture absorption. Temperature fluctuations were always deemed as damaging to composite structures. Due to the haziness still present in current research coupled with the importance of composite applications, the need for additional investigation in this topic is urgently obvious. All in all, future studies are encouraged to tabulate test procedures and results in an effort to minimize the current ambiguities in the aging information accumulated thus far.by Margie N. Treviño-Garrido.S.B

La resolución de problemas y la educación en ciencias naturales

Esta investigación se centrará en la delimitación de los conceptos problema y resolución de problemas, así como en una aproximación a los tipos de problemas, en el marco de esta línea del Programa Interinstitucional de Doctorado en Educación, en el área de Ciencias Naturales

A One Health overview, facilitating advances in comparative medicine and translational research.

Table of contentsA1 One health advances and successes in comparative medicine and translational researchCheryl StroudA2 Dendritic cell-targeted gorilla adenoviral vector for cancer vaccination for canine melanomaIgor Dmitriev, Elena Kashentseva, Jeffrey N. Bryan, David T. CurielA3 Viroimmunotherapy for malignant melanoma in the companion dog modelJeffrey N. Bryan, David Curiel, Igor Dmitriev, Elena Kashentseva, Hans Rindt, Carol Reinero, Carolyn J. HenryA4 Of mice and men (and dogs!): development of a commercially licensed xenogeneic DNA vaccine for companion animals with malignant melanomaPhilip J. BergmanA5 Successful immunotherapy with a recombinant HER2-expressing Listeria monocytogenes in dogs with spontaneous osteosarcoma paves the way for advances in pediatric osteosarcomaNicola J. Mason, Josephine S. Gnanandarajah, Julie B. Engiles, Falon Gray, Danielle Laughlin, Anita Gaurnier-Hausser, Anu Wallecha, Margie Huebner, Yvonne PatersonA6 Human clinical development of ADXS-HER2Daniel O'ConnorA7 Leveraging use of data for both human and veterinary benefitLaura S. TremlA8 Biologic replacement of the knee: innovations and early clinical resultsJames P. StannardA9 Mizzou BioJoint Center: a translational success storyJames L. CookA10 University and industry translational partnership: from the lab to commercializationMarc JacobsA11 Beyond docking: an evolutionarily guided OneHealth approach to drug discoveryGerald J. Wyckoff, Lee Likins, Ubadah Sabbagh, Andrew SkaffA12 Challenges and opportunities for data applications in animal health: from precision medicine to precision husbandryAmado S. GuloyA13 A cloud-based programmable platform for healthHarlen D. HaysA14 Comparative oncology: One Health in actionAmy K. LeBlancA15 Companion animal diseases bridge the translational gap for human neurodegenerative diseaseJoan R. Coates, Martin L. Katz, Leslie A. Lyons, Gayle C. Johnson, Gary S. Johnson, Dennis P. O'BrienA16 Duchenne muscular dystrophy gene therapyDongsheng DuanA17 Polycystic kidney disease: cellular mechanisms to emerging therapiesJames P. CalvetA18 The domestic cat as a large animal model for polycystic kidney diseaseLeslie A. Lyons, Barbara GandolfiA19 The support of basic and clinical research by the Polycystic Kidney Disease FoundationDavid A. BaronA20 Using naturally occurring large animal models of human disease to enable clinical translation: treatment of arthritis using autologous stromal vascular fraction in dogsMark L. WeissA21 Regulatory requirements regarding clinical use of human cells, tissues, and tissue-based productsDebra A. WebsterA22 Regenerative medicine approaches to Type 1 diabetes treatmentFrancis N. KaranuA23 The zoobiquity of canine diabetes mellitus, man's best friend is a friend indeed-islet transplantationEdward J. RobbA24 One Medicine: a development model for cellular therapy of diabetesRobert J. Harman

Humoral Immune Response to Messenger RNA COVID-19 Vaccines Among Patients With Inflammatory Bowel Disease

Early indicators suggest that highly effective messenger RNA (mRNA) vaccinations (BNT 162b2 [Pfizer-BioNTech] and mRNA-1273 [NIH-Moderna]) are beginning to curb the coronavirus disease 2019 (COVID-19) pandemic that has already taken the lives of 3 million individuals worldwide. However, questions about vaccine effectiveness remain for individuals with inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, who are frequently treated with immune suppression. An early study among transplant recipients indicated low humoral immune response after an initial vaccination, in contrast to the robust response observed in healthy individuals in phase III clinical trials. Similarly, a recent study in IBD patients suggested that treatment with infliximab is associated with an attenuated level of anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike after a single dose of mRNA vaccine

Antimicrobial Resistant Salmonella enterica Typhimurium Colonizing Chickens: The Impact of Plasmids, Genotype, Bacterial Communities, and Antibiotic Administration on Resistance

The rise in Salmonella resistance to cephalosporins and fluoroquinolones has become a significant threat to public health. At issue, is whether agricultural use of antimicrobials is selecting antibiotic resistance in Salmonella and the degree to which large antimicrobial resistance gene reservoirs, present in animal manures, contribute to this resistance. Two in vivo studies were performed to address these questions. In the first study, chickens were administered Salmonella and commensals, including an Escherichia coli strain with a mobile, ceftiofur-resistance plasmid, in order to determine how antibiotic administration impacted resistance in E. coli and Salmonella. All antibiotics administered to chickens increased streptomycin resistance in E. coli. However, only ceftiofur administration increased resistance in Salmonella and specifically to extended-spectrum β-lactams and cephalosporins (ESBL). There was no significant increase in ESBL-resistant Salmonella in chickens administered a ceftiofur-resistance plasmid donor. In the second study, chickens were administered two different isolates of S. enterica Typhimurium and a chicken resistome to serve as a gene donor. Birds were subsequently administered chlortetracycline or streptomycin. Antimicrobial administration significantly altered aminoglycoside and tetracycline resistance in the Enterobacteriaceae population. However, there was no significant increase in antimicrobial resistant Salmonella. Administration of a chicken resistome had no significant impact on prevalence of resistance in Enterobacteriaceae populations, including Salmonella. Evident, from both studies, was that these treatments had minimal effect on increasing the prevalence of resistance in Salmonella, suggesting that other factors may be more important in dissemination of antimicrobial resistant Salmonella in chickens

Tumor necrosis factor-α and interferon-γ stimulate MUC16 (CA125) expression in breast, endometrial and ovarian cancers through NFκB

Transmembrane mucins (TMs) are restricted to the apical surface of normal epithelia. In cancer, TMs not only are over-expressed, but also lose polarized distribution. MUC16/CA125 is a high molecular weight TM carrying the CA125 epitope, a well-known molecular marker for human cancers. MUC16 mRNA and protein expression was mildly stimulated by low concentrations of TNFα (2.5 ng/ml) or IFNγ (20 IU/ml) when used alone; however, combined treatment with both cytokines resulted in a moderate (3-fold or less) to large (> 10-fold) stimulation of MUC16 mRNA and protein expression in a variety of cancer cell types indicating that this may be a general response. Human cancer tissue microarray analysis indicated that MUC16 expression directly correlates with TNFα and IFNγ staining intensities in certain cancers. We show that NFκB is an important mediator of cytokine stimulation of MUC16 since siRNA-mediated knockdown of NFκB/p65 greatly reduced cytokine responsiveness. Finally, we demonstrate that the 250 bp proximal promoter region of MUC16 contains an NFκB binding site that accounts for a large portion of the TNFα response. Developing methods to manipulate MUC16 expression could provide new approaches to treating cancers whose growth or metastasis is characterized by elevated levels of TMs, including MUC16

Conducting Online Expert panels: a feasibility and experimental replicability study

<p>Abstract</p> <p>Background</p> <p>This paper has two goals. First, we explore the feasibility of conducting online expert panels to facilitate consensus finding among a large number of geographically distributed stakeholders. Second, we test the replicability of panel findings across four panels of different size.</p> <p>Method</p> <p>We engaged 119 panelists in an iterative process to identify definitional features of Continuous Quality Improvement (CQI). We conducted four parallel online panels of different size through three one-week phases by using the RAND's ExpertLens process. In Phase I, participants rated potentially definitional CQI features. In Phase II, they discussed rating results online, using asynchronous, anonymous discussion boards. In Phase III, panelists re-rated Phase I features and reported on their experiences as participants.</p> <p>Results</p> <p>66% of invited experts participated in all three phases. 62% of Phase I participants contributed to Phase II discussions and 87% of them completed Phase III. Panel disagreement, measured by the mean absolute deviation from the median (MAD-M), decreased after group feedback and discussion in 36 out of 43 judgments about CQI features. Agreement between the four panels after Phase III was fair (four-way kappa = 0.36); they agreed on the status of five out of eleven CQI features. Results of the post-completion survey suggest that participants were generally satisfied with the online process. Compared to participants in smaller panels, those in larger panels were more likely to agree that they had debated each others' view points.</p> <p>Conclusion</p> <p>It is feasible to conduct online expert panels intended to facilitate consensus finding among geographically distributed participants. The online approach may be practical for engaging large and diverse groups of stakeholders around a range of health services research topics and can help conduct multiple parallel panels to test for the reproducibility of panel conclusions.</p

Heritability of fractional anisotropy in human white matter: a comparison of Human Connectome Project and ENIGMA-DTI data

The degree to which genetic factors influence brain connectivity is beginning to be understood. Large-scale efforts are underway to map the profile of genetic effects in various brain regions. The NIH-funded Human Connectome Project (HCP) is providing data valuable for analyzing the degree of genetic influence underlying brain connectivity revealed by state-of-the-art neuroimaging methods. We calculated the heritability of the fractional anisotropy (FA) measure derived from diffusion tensor imaging (DTI) reconstruction in 481 HCP subjects (194/287 M/F) consisting of 57/60 pairs of mono- and dizygotic twins, and 246 siblings. FA measurements were derived using (Enhancing NeuroImaging Genetics through Meta-Analysis) ENIGMA DTI protocols and heritability estimates were calculated using the SOLAR-Eclipse imaging genetic analysis package. We compared heritability estimates derived from HCP data to those publicly available through the ENIGMA-DTI consortium, which were pooled together from five-family based studies across the US, Europe, and Australia. FA measurements from the HCP cohort for eleven major white matter tracts were highly heritable (h2 = 0.53–0.90, p < 10− 5), and were significantly correlated with the joint-analytical estimates from the ENIGMA cohort on the tract and voxel-wise levels. The similarity in regional heritability suggests that the additive genetic contribution to white matter microstructure is consistent across populations and imaging acquisition parameters. It also suggests that the overarching genetic influence provides an opportunity to define a common genetic search space for future gene-discovery studies. Uniquely, the measurements of additive genetic contribution performed in this study can be repeated using online genetic analysis tools provided by the HCP ConnectomeDB web application