What Development Regulatory Variables Say—or Don’t Say—About A Municipality

Little is known about how regulatory development variables reflect and define a community. This paper explores the correlation of development regulatory variables with broader community measures in 68 municipalities in the Twin Cities area of Minnesota. Coefficients of determination, correlation coefficients, principal component analysis, and factor analysis were used to compare development regulatory data with broader municipal measures. The hypothesis tested is overarching: that a municipality’s development regulations and processes correlate to general measures of community composition. The strongest and only significant correlations found were in the municipal use of tax increment financing and commercial/ industrial property values, non-residential construction activity, population, and multi-family building permit activity.

A 3D real time rendering engine for binaural sound reproduction

Proceedings of the 9th International Conference on Auditory Display (ICAD), Boston, MA, July 7-9, 2003.A method of computationally efficient 3D sound reproduction via headphones is presented using a virtual Ambisonic approach. Previous studies have shown that incorporating head tracking as well as room simulation is important to improve sound source localization capabilities. The simulation of virtual acoustic space requires to filter the stimuli with head related transfer functions (HRTFs). In time-varying systems this yields the problem of high quality interpolation between different HRTFs. The proposed model states that encoding signals into Ambisonic domain results in time-invariant HRTF filters. The proposed system is implemented on a usual notebook using Pure Data (PD), a graphically based open source real time computer music software

Processing of the papain precursor. Purification of the zymogen and characterization of its mechanism of processing.

The precursor of the cysteine protease papain has been expressed and secreted as propapain from insect cells infected with a recombinant baculovirus expressing a synthetic gene coding for prepropapain. This 39-kDa secreted propapain zymogen molecule is glycosylated and can be processed in vitro into an enzymatically active authentic papain molecule of 24.5 kDa (Vernet, T., Tessier, D.C., Richardson, C., Laliberte, F., Khouri, H. E., Bell, A. W., Storer, A. C., and Thomas, D. Y. (1990) J. Biol. Chem. 265, 16661-16666). Recombinant propapain was stabilized with Hg2+ and purified to homogeneity using affinity chromatography, gel filtration, and ion-exchange chromatographic procedures. The maximum rate of processing in vitro was achieved at approximately pH 4.0, at a temperature of 65 degrees C and under reducing conditions. Precursor processing is inhibited by a variety of reversible and irreversible cysteine protease inhibitors but not by specific inhibitors of serine, metallo or acid proteases. Replacement by site-directed mutagenesis of the active site cysteine with a serine at position 25 also prevents processing. The inhibitor 125I-N-(2S,3S)-3-trans-hydroxycarbonyloxiran-2-carbonyl-L-tyrosine benzyl ester covalently labeled the wild type papain precursor, but not the C25S mutant, indicating that the active site is accessible to the inhibitor and is in a native conformation within the precursor. Based on biochemical and kinetic analyses of the activation and processing of propapain we have shown that the papain precursor is capable of autoproteolytic cleavage (intramolecular). Once free papain is released processing can then occur in trans (intermolecular)

The CitySPIN Platform: A CPSS Environment for City-Wide Infrastructures

Cyber-physical Social System (CPSS) are complex systems that span the boundaries of the cyber, physical and social spheres. They play an important role in a variety of domains ranging from industry to smart city applications. As such, these systems necessarily need to take into account, combine and make sense of heterogeneous data sources from legacy systems, from the physical layer and also the social groups that are part of/use the system. The collection, cleansing and integration of these data sources represents a major effort not only during the operation of the system, but also during its engineering and design. Indeed, while ongoing efforts are concerned primarily with the operation of such systems, limited focus has been put on supporting the engineering phase of CPSS. To address this shortcoming, within the CitySPIN project we aim to create a platform that supports stakeholders involved in the design of these systems especially in terms of support for data management. To that end, we develop methods and techniques based on Semantic Web and Linked Data technologies for the acquisition and integration of heterogeneous data from disparate structured, semi-structured and unstructured sources, including open data and social data. In this paper we present the overall system architecturewith a core focus on data acquisition and integration.We demon-strate our approach through a prototypical implementation of an adaptive planning use case for public transportation scheduling

Focused Ion Beam Microfabrication

Contains an introduction, reports on x research projects and a list of publications.Defense Advanced Research Projects Agency/U.S. Army Research Office Grant DAAL-03-92-G-0217National Science Foundation Grant ECS 89-21728Defense Advanced Research Projects Agency/U.S. Army Research Office (ASSERT Program) Grant DAAL03-92-G-0305Semiconductor Research CorporationNational Science Foundation Grant DMR 92-02633U.S. Army Research Office Grant DAAL03-90-G-0223U.S. Navy - Naval Research Laboratory/Micrion Contract M0877

Focused Ion Beam Microfabrication

Contains an introduction, reports on seven research projects and a list of publications.Defense Advanced Research Projects Agency/U.S. Army Research Office Contract DAAL03-88-K-0108National Science Foundation Grant ECS 89-21728U.S. Army Research Office Contract DAAL03-87-K-0126U.S. Navy - Naval Research Laboratory/Micrion Agreement M08774SEMATEC

Focused Ion Beam Fabrication

Contains reports on thirteen research projects and a list of publications.Defense Advanced Research Projects Agency/U.S. Army Research Office Contract DAAL03-88-K-0108National Science Foundation Grant ECS 89-21728MIT Lincoln Laboratory Innovative Research ProgramSEMATECH Contract 90-MC-503Micrion Contract M08774U.S. Army Research Office Contract DAAL03-87-K-0126IBM Corporatio

The potency of the fs260 connexin43 mutant to impair keratinocyte differentiation is distinct from other disease-linked connexin43 mutants

Although there are currently 62 mutants of Cx43 (connexin43) that can cause ODDD (oculodentodigital dysplasia), only two mutants have also been reported to cause palmar plantar hyperkeratosis. To determine how mutants of Cx43 can lead to this skin disease, REKs (rat epidermal keratinocytes) were engineered to express an ODDD-associated Cx43 mutant always linked to skin disease (fs260), an ODDD-linked Cx43 mutant which has been reported to sometimes cause skin disease (fs230), Cx43 mutants which cause ODDD only (G21R, G138R), a mouse Cx43 mutant linked to ODDD (G60S), a non-disease-linked truncated Cx43 mutant that is trapped in the endoplasmic reticulum (Δ244*) or full-length Cx43. When grown in organotypic cultures, of all the mutants investigated, only the fs260-expressing REKs consistently developed a thinner stratum corneum and expressed lower levels of Cx43, Cx26 and loricrin in comparison with REKs overexpressing wild-type Cx43. REKs expressing the fs260 mutant also developed a larger organotypic vital layer after acetone-induced injury and exhibited characteristics of parakeratosis. Collectively, our results suggest that the increased skin disease burden exhibited in ODDD patients harbouring the fs260 mutant is probably due to multiple additive effects cause by the mutant during epidermal differentiation

Realizing General Education: Reconsidering Conceptions and Renewing Practice

General Education is widely touted as an enduring distinctive of higher education in the United States (Association of American Colleges and Universities, [11]; Boyer, [37]; Gaston, [86]; Zakaria, [202]). The notion that undergraduate education demands wide‐ranging knowledge is a hallmark of U.S. college graduates that international educators emulate (Blumenstyk, [25]; Rhodes, [158]; Tsui, [181]). The veracity of this distinct educational vision is supported by the fact that approximately one third of the typically 120 credits required for the bachelor\u27s degree in the United States consist of general education courses (Lattuca & Stark, [120]). Realizing a general education has been understood to be central to achieving higher education\u27s larger purposes, making it a particularly salient concern