Auditory cueing strategy for stride length and cadence modification: a feasibility study with healthy adults

People with Parkinson's Disease experience gait impairments that significantly impact their quality of life. Visual, auditory, and tactile cues can alleviate gait impairments, but they can become less effective due to the progressive nature of the disease and changes in people's motor capability. In this study, we develop a human-in-the-loop (HIL) framework that monitors two key gait parameters, stride length and cadence, and continuously learns a person-specific model of how the parameters change in response to the feedback. The model is then used in an optimization algorithm to improve the gait parameters. This feasibility study examines whether auditory cues can be used to influence stride length in people without gait impairments. The results demonstrate the benefits of the HIL framework in maintaining people's stride length in the presence of a secondary task.Comment: 6 pages, 4 figures; the paper is accepted and presented at EMBC 202

The Influence of a Biologically Relevant Substratum Topography on Human Aortic and Umbilical Vein Endothelial Cells

AbstractA topographically patterned substrate with stochastic surface order that closely mimics the topographic features of native basement membranes has been fabricated to investigate the influence of topographic biophysical cueing on human aortic and umbilical vein endothelial cells. The stochastic substrate was fabricated by first generating a highly porous polyelectrolyte multilayer film of poly(acrylic acid) and poly(allylamine hydrochloride) followed by replicate production of this biomimetic topography via soft lithography. These substrates, which are easy to prepare and replicate, possess a number of prominent features associated with in vivo vascular basement membrane (interwoven ridges and grooves, bumps, and pores), which have typically been studied as singular features that frequently possess anisotropic surface order (e.g., alternating ridges and grooves). When compared to a flat surface of identical chemistry, these biomimetic topographies influenced a number of important cellular behaviors associated with the homeostasis and degradation of vascular tissues. These include modulating cell migration rate and directional persistence, proliferation rate, and gene expression associated with regulation and remodeling of vascular tissues as well as inflammation

Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer.

Recent advances in thefield of immuno-oncology have brought transformative changes in the management ofcancer patients. The immune profile of tumours has been found to have key value in predicting disease prognosis andtreatment response in various cancers. Multiplex immunohistochemistry and immunofluorescence have emerged aspotent tools for the simultaneous detection of multiple protein biomarkers in a single tissue section, therebyexpanding opportunities for molecular and immune profiling while preserving tissue samples. By establishing thephenotype of individual tumour cells when distributed within a mixed cell population, the identification of clinicallyrelevant biomarkers with high-throughput multiplex immunophenotyping of tumour samples has great potential toguide appropriate treatment choices. Moreover, the emergence of novel multi-marker imaging approaches can nowprovide unprecedented insights into the tumour microenvironment, including the potential interplay betweenvarious cell types. However, there are significant challenges to widespread integration of these technologies in dailyresearch and clinical practice. This review addresses the challenges and potential solutions within a structuredframework of action from a regulatory and clinical trial perspective. New developments within thefield ofimmunophenotyping using multiplexed tissue imaging platforms and associated digital pathology are also described,with a specific focus on translational implications across different subtypes of cancer

Quasars probing intermediate redshift star-forming galaxies

We present a sample of 46 [OIII]-emitting galaxies at z<0.8 detected in the fibre spectra of quasars from the SDSS-DR7 through an automatic search procedure. We also detect [OII] and Hb emission lines from most of these galaxies in the SDSS spectra. We study both the emission and absorption properties of a sub-sample of 17 galaxies in the redshift range z=0.4-0.7, where MgII lines are covered by the SDSS spectra. The measured lower-limits on the star-formation rates of these galaxies are in the range 0.2-20 M_sun/yr. The emission line luminosities and (O/H) metallicities from R23 measured in this sample are similar to what is found in normal galaxies at these redshifts. Thus, this constitutes a unique sample of intermediate redshift star-forming galaxies where we can study the QSO absorber - galaxy connection. Strong MgII (W>1A) as well as MgI absorption lines are detected in the QSO spectra at the redshift of most of these galaxies. Strong FeII (W>1A) absorption lines are also generally detected whenever the appropriate wavelength ranges are covered. This suggests that most of these systems could be bona-fide Damped Lyman-alpha systems. We investigate various possible relations between the MgII rest equivalent widths and the emission line properties. We find a possible (2 sigma) correlation between the emission-line metallicity of the galaxies and the MgII rest equivalent width of the absorbers [truncated].Comment: 15 pages, 11 figures, 5 tables. accepted for publication in MNRA

A review of physical supply and EROI of fossil fuels in China

This paper reviews China’s future fossil fuel supply from the perspectives of physical output and net energy output. Comprehensive analyses of physical output of fossil fuels suggest that China’s total oil production will likely reach its peak, at about 230 Mt/year (or 9.6 EJ/year), in 2018; its total gas production will peak at around 350 Bcm/year (or 13.6 EJ/year) in 2040, while coal production will peak at about 4400 Mt/year (or 91.9 EJ/year) around 2020 or so. In terms of the forecast production of these fuels, there are significant differences among current studies. These differences can be mainly explained by different ultimately recoverable resources assumptions, the nature of the models used, and differences in the historical production data. Due to the future constraints on fossil fuels production, a large gap is projected to grow between domestic supply and demand, which will need to be met by increasing imports. Net energy analyses show that both coal and oil and gas production show a steady declining trend of EROI (energy return on investment) due to the depletion of shallow-buried coal resources and conventional oil and gas resources, which is generally consistent with the approaching peaks of physical production of fossil fuels. The peaks of fossil fuels production, coupled with the decline in EROI ratios, are likely to challenge the sustainable development of Chinese society unless new abundant energy resources with high EROI values can be found

Global proteomics analysis of the response to starvation in <i>C. elegans</i>

Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in the abundance of over 5,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin-associated proteins, including specific linker histones, histone variants, and histone posttranslational modifications associated with the epigenetic control of gene expression. To maximize community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/)

Genetically Engineered Phages: a Review of Advances over the Last Decade

Summary: Soon after their discovery in the early 20th century, bacteriophages were recognized to have great potential as antimicrobial agents, a potential that has yet to be fully realized. The nascent field of phage therapy was adversely affected by inadequately controlled trials and the discovery of antibiotics. Although the study of phages as anti-infective agents slowed, phages played an important role in the development of molecular biology. In recent years, the increase in multidrug-resistant bacteria has renewed interest in the use of phages as antimicrobial agents. With the wide array of possibilities offered by genetic engineering, these bacterial viruses are being modified to precisely control and detect bacteria and to serve as new sources of antibacterials. In applications that go beyond their antimicrobial activity, phages are also being developed as vehicles for drug delivery and vaccines, as well as for the assembly of new materials. This review highlights advances in techniques used to engineer phages for all of these purposes and discusses existing challenges and opportunities for future work.D.P.P. acknowledges financial support from the Portuguese Foundation for Science and Technology (FCT) through grant SFRH/BD/76440/2011. This work was funded by The Center for Microbiome Informatics and Therapeutics and NSF Expeditions in Computing Program award #1522074 as part of the Living Computing Project. This work was further supported by grants from the Defense Threat Reduction Agency (grants HDTRA1-14-1-0007 and HDTRA1-15-1-0050), the National Institutes of Health (grants 1DP2OD008435,1P50GM098792,1R01EB017755, and 1R21AI12166901), and the U.S. Army Research Laboratory and U.S. Army Research Office, through the Institute for Soldier Nanotechnologies, under contract number W911NF-13-D-0001.S.S.is an FCT investigator (IF/01413/2013). D.P.P., S.S., and J.A. also acknowledge financial support from FCT under the scope of the strategic funding of the UID/ BIO/04469/2013 unit and COMPETE 2020 (grant POCI-01-0145FEDER-006684). T.K.L. is a founder of Sample6 Inc. and Eligo Biosciences, two companies developing phage-based technologies

MOIRCS Deep Survey. VIII. Evolution of Star Formation Activity as a Function of Stellar Mass in Galaxies since z~3

We study the evolution of star formation activity of galaxies at 0.5<z<3.5 as a function of stellar mass, using very deep NIR data taken with Multi-Object Infrared Camera and Spectrograph (MOIRCS) on the Subaru telescope in the GOODS-North region. The NIR imaging data reach K ~ 23-24 Vega magnitude and they allow us to construct a nearly stellar mass-limited sample down to ~ 10^{9.5-10} Msun even at z~3. We estimated star formation rates (SFRs) of the sample with two indicators, namely, the Spitzer/MIPS 24um flux and the rest-frame 2800A luminosity. The SFR distribution at a fixed Mstar shifts to higher values with increasing redshift at 0.5<z<3.5. More massive galaxies show stronger evolution of SFR at z>~1. We found galaxies at 2.5<z<3.5 show a bimodality in their SSFR distribution, which can be divided into two populations by a constant SSFR of ~2 Gyr^{-1}. Galaxies in the low-SSFR group have SSFRs of ~ 0.5-1.0 Gyr^{-1}, while the high-SSFR population shows ~10 Gyr^{-1}. The cosmic SFRD is dominated by galaxies with Mstar = 10^{10-11} Msun at 0.5<z<3.5, while the contribution of massive galaxies with Mstar = 10^{11-11.5} Msun shows a strong evolution at z>1 and becomes significant at z~3, especially in the case with the SFR based on MIPS 24um. In galaxies with Mstar = 10^{10-11.5} Msun, those with a relatively narrow range of SSFR (<~1 dex) dominates the cosmic SFRD at 0.5<z<3.5. The SSFR of galaxies which dominate the SFRD systematically increases with redshift. At 2.5<z<3.5, the high-SSFR population, which is relatively small in number, dominates the SFRD. Major star formation in the universe at higher redshift seems to be associated with a more rapid growth of stellar mass of galaxies.Comment: 16 pages, 13 figures, accepted for publication in Ap