Numerical methods for solving ordinary and partial differential equations

The numerical solution of ordinary differential equations will be the main topics discussed in the first half of this thesis. In Chapter 2 initial value problems are examined and the resulting differential equations are solved by using extrapolation techniques. Computer trials of the algorithm are completed by a special ordinary differential equation tester program and the statistics of its performance are compared with other established methods. In the following Chapter the relevant theory and properties, associated with Chebyshev polynomials, is presented. Then boundary value problems are solved by representing the ordinary differential equations as a Chebyshev series. Finally, similar methods are developed for the solution of partial differential equations

Car-Park Management using Wireless Sensor Networks

A complete wireless sensor network solution for car-park management is presented in this paper. The system architecture and design are first detailed, followed by a description of the current working implementation, which is based on our DSYS25z sensing nodes. Results of a series of real experimental tests regarding connectivity, sensing and network performance are then discussed. The analysis of link characteristics in the car park scenario shows unexpected reliability patterns which have a strong influence on MAC and routing protocol design. Two unexpected link reliability patterns are identified and documented. First, the presence of the objects (cars) being sensed can cause significant interference and degradation in communication performance. Second, link quality has a high temporal correlation but a low spatial correlation. From these observations we conclude that a) the construction and maintenance of a fixed topology is not useful and b) spatial rather than temporal message replicates can improve transport reliability

Biodegradation of Amphipathic Fluorinated Peptides Reveals a New Bacterial Defluorinating Activity and a New Source of Natural Organofluorine Compounds

Three peptides comprising mono-, di-, and tri-fluoroethylglycine (MfeGly, DfeGly, and TfeGly) residues alternating with lysine were digested by readily available proteases (elastase, bromelain, trypsin, and proteinase K). The degree of degradation depended on the enzyme employed and the extent of fluorination. Incubation of the peptides with a microbial consortium from garden soil resulted in degradation, yielding fluoride ions. Further biodegradation studies conducted with the individual fluorinated amino acids demonstrated that the degree of defluorination followed the sequence MfeGly > DfeGly > TfeGly. Enrichment of the soil bacteria employing MfeGly as a sole carbon and energy source resulted in the isolation of a bacterium, which was identified as Serratia liquefaciens. Cell-free extracts of this bacterium enzymatically defluorinated MfeGly, yielding fluoride ion and homoserine. In silico analysis of the genome revealed the presence of a gene that putatively codes for a dehalogenase. However, the low overall homology to known enzymes suggests a potentially new hydrolase that can degrade monofluorinated compounds. 19F NMR analysis of aqueous soil extracts revealed the unexpected presence of trifluoroacetate, fluoride ion, and fluoroacetate. Growth of the soil consortium in tryptone soya broth supplemented with fluoride ions resulted in fluoroacetate production; thus, bacteria in the soil produce and degrade organofluorine compounds

Biotransformation of fluorophenyl pyridine carboxylic acids by the model fungus Cunninghamella elegans

1. Fluorine plays a key role in the design of new drugs and recent FDA approvals included two fluorinated drugs, tedizolid phosphate and vorapaxar, both of which contain the fluorophenyl pyridyl moiety. 2. To investigate the likely phase-I (oxidative) metabolic fate of this group, various fluorinated phenyl pyridine carboxylic acids were incubated with the fungus Cunninghamella elegans, which is an established model of mammalian drug metabolism. 3. 19F NMR spectroscopy established the degree of biotransformation, which varied depending on the position of fluorine substitution, and gas chromatography–mass spectrometry (GC–MS) identified alcohols and hydroxylated carboxylic acids as metabolites. The hydroxylated metabolites were further structurally characterised by nuclear magnetic resonance spectroscopy (NMR), which demonstrated that hydroxylation occurred on the 4′ position; fluorine in that position blocked the hydroxylation. 4. The fluorophenyl pyridine carboxylic acids were not biotransformed by rat liver microsomes and this was a consequence of inhibitory action, and thus, the fungal model was crucial in obtaining metabolites to establish the mechanism of catabolism

Isolation and characterisation of actinomycin D producing Streptomyces spp. from Sudanese soil

Sudanese soil is an unexplored source of antibiotic-producing microorganisms. Here, we reported the screening of soil samples from Sudan for actinomycetes that have antibacterial activity. Two isolates, AH11.4 and AH47, displaying a broad antimicrobial spectrum were selected for further study. Morphological, physiological and biochemical studies indicated that the two isolates are Streptomyces spp. 16S rDNA sequencing confirmed that the closest matches were to other Streptomyces, but phylogenetic analysis demonstrated that the Sudanese strains were on a different node to previously identified strains. The antibiotic activity was isolated by preparative High-performance liquid chromatography (HPLC) and determined to be primarily actinomycin D on the basis of UV, 1H- and 13CNMR, and MS analyses. One strain also produced actinomycin X2 and aB. These strains are distinct from the known producers of actinomycin, thus are new sources of these antibiotics.Keywords: Screening, antibiotic, antitumour, identificationAfrican Journal of Biotechnology Vol. 12(19), pp. 2624-263

Autonomous unmanned aerial vehicle for search and rescue using software defined radio

To find missing people in a remote area, we propose an autonomous unmanned aerial vehicle (UAV) approach which attempts to locate the target by detecting and localising the radio signals produced by a GSM cell phone. By using a low- weight software defined radio and companion computer, the UAV can act as a GSM base station and induce the missing person's device to attempt to make contact. Through the signal strength values and known UAV location, a series of these contact attempts can be used to quickly and accurately localise their position. As the area in which the missing person might be located may be quite large, and the interaction of radio signals with terrain is potentially complex, an efficient search strategy for exploring the area is required in order to reduce time taken to make contact. We make use of a constraint-based graph-based path planning approach to produce a route for the UAV to traverse in the air passing through expected signals from a large number of possible source locations, and demonstrate through experiments the timely identification and localisation of the cell phone

Simultaneous removal of malachite green and hexavalent chromium by Cunninghamella elegans biofilm in a semi-continuous system

The present study was conducted to evaluate the potential of the fungus Cunninghamella elegans for simultaneous decolourisation of a triphenylmethane dye malachite green (MG) and hexavalent chromium [Cr(VI)] in the same media. This fungus can degrade MG through its reduction into leucomalachite green and then demethylation followed by oxidative cleavage. Along with MG degradation, C. elegans biofilm could effectively and repeatedly remove Cr(VI) from the liquid cultures even in the presence of high concentrations (40 g L−1) of NaCl and various other metal ions. C. elegans biofilm was also found to adsorb different dyes (reactive black-5, acid orange 7, direct red 81 and brilliant blue G) concurrently with Cr(VI). Based on its potential for simultaneous removal of dyes and Cr(VI) as well as reusability, C. elegans biofilm is envisaged as an efficient bioresource to devise strategies for treatment of wastewaters loaded with multiple pollutants.Environmental Protection AgencyIrish Research Counci