ACUTE EFFECTS OF MASSAGE ON PASSIVE ANKLE STIFFNESS FOLLOWING AN EXHAUSTIVE STRET CHSHORTEN CYCLE TASK: A PILOT STUDY

This preliminary study evaluated the effect of massage on the passive stiffness of the calf muscle complex following single-leg hopping to volitional exhaustion. Four young and healthy male participants had their ankle taken through full joint excursion to determine the resistance to ankle dorsiflexion both prior to and immediately following hopping. A 10 minute rest and massage were then applied in a random order and follow up measures taken immediately Mer each intervention. Calf muscle stiffness increased in three of the four participants following hopping and two participants had a decrease following massage. This study suggests that changes in calf muscle stiffness are sensitive to both exercise and massage. It is important to determine the underlying mechanism(s) to changes in calf stiffness following exercise and whether massage offers any benefit

THE EFFECT OF FATIGUE ON LOWER LIMB MOTOR VARIABILITY DURING A CONTROLLED REPETITIVE STRETCH-SHORTEN CYCLE TASK

This study evaluated changes in lower limb joint coupling variability during single-leg hopping to exhaustion. Twenty recreationally active male and female participants performed single-leg hopping at 2.2 Hz to a target height. At 0, 20, 40, 60, 80 and 100% of the total duration of hopping, spatio-temporal characteristics and variability of the kneeankle (KA) and hip-knee (HK) joint couplings were determined. There was a significant increase in variability of the KA and HK joint couplings in the flexion-extension axes during the loading and propulsion phases as hopping progressed. However, there was maintenance of performance output characteristics throughout the task. These findings suggest that changes in joint coupling variability may be a compensatory strategy to allow continuous single-leg hopping as the effects of muscular fatigue increase

DOES THE METHOD OF MEASURING CENTRE OF MASS DISPLACEMENT AFFECT VERTICAL STIFFNESS CALCULATION IN SINGLE-LEG HOPPING?

The purpose of this study was to compare vertical stiffness values calculated from two kinetic and two kinematic estimations of the vertical displacement of the centre of mass. Twenty recreationally active male and female participants completed one 15 s single-leg hopping trial at 2.2 Hz with vertical stiffness calculated for the first 10 complete hop cycles. Vertical displacement was estimated using double integration (DI), first principle (FP), sacral marker cluster (SMC) and segmental analysis (SA) methods. Bland-Altman plots demonstrated the SA and DI methods to have a small bias (0.92 kN/m) and tight 95% limits of agreement (-1.16 to 3.08 kN/m). In contrast, the SMC and FP methods underestimated and overestimated vertical stiffness, respectively. These findings suggest the SA and DI methods can be used interchangeably to calculate vertical stiffness

SEX DIFFERENCES IN LOWER LIMB MOVEMENT VARIABILITY DURING A FATIGUING REPETITIVE LOADING TASK

This study evaluated differences in lower limb joint coupling variability between recreationally-active male (n = 21) and female participants (n = 20) during single-leg hopping to exhaustion. Spatio-temporal characteristics and variability of the knee-ankle and hip-knee joint couplings were determined over the duration of hopping. As fatigue progressed joint coupling variability increased by a greater magnitude in females compared to males. Females had significantly lower variability compared to males in the knee-ankle couplings during the propulsion phase at the beginning of the trial but this effect progressively disappeared during the trial. These findings suggest that as fatigue progresses, there is a regression to a similar magnitude of joint coupling variability which may represent a common level of synchronous joint interaction between sexes

A RANDOMISED CONTROLLED TRIAL EVALUATING THE EFFECTS OF COMPRESSION GARMENTS DURING SINGLE-LEG HOPPING TO EXHAUSTION

The purpose of this investigation was to determine the effect of compression garments on spatiotemporal and leg mechanical characteristics during single-leg-hopping (2.2 Hz) to volitional exhaustion. This study demonstrated that compression garments had no significant effect on leg mechanical characteristics or performance parameters of single-leg hopping to volitional exhaustion. There was a significant increase in the duration of the loading phase and decrease in the flight phase from the start to the end during single-leg hopping task indicating that there may have been a shift in the motor control strategy used to preserve vertical leg stiffness and hopping frequency in a repeated and rapid loading task

How Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) progresses. The natural history of ME/CFS

We propose a framework for understanding and interpreting the pathophysiology of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) that considers wider determinants of health and long-term temporal variation in pathophysiological features and disease phenotype throughout the natural history of the disease. As in other chronic diseases, ME/CFS evolves through different stages, from asymptomatic predisposition, progressing to a prodromal stage, and then to symptomatic disease. Disease incidence depends on genetic makeup and environment factors, the exposure to singular or repeated insults, and the nature of the host response. In people who develop ME/CFS, normal homeostatic processes in response to adverse insults may be replaced by aberrant responses leading to dysfunctional states. Thus, the predominantly neuro-immune manifestations, underlined by a hyper-metabolic state, that characterize early disease, may be followed by various processes leading to multi-systemic abnormalities and related symptoms. This abnormal state and the effects of a range of mediators such as products of oxidative and nitrosamine stress, may lead to progressive cell and metabolic dysfunction culminating in a hypometabolic state with low energy production. These processes do not seem to happen uniformly; although a spiraling of progressive inter-related and self-sustaining abnormalities may ensue, reversion to states of milder abnormalities is possible if the host is able to restate responses to improve homeostatic equilibrium. With time variation in disease presentation, no single ME/CFS case description, set of diagnostic criteria, or molecular feature is currently representative of all patients at different disease stages. While acknowledging its limitations due to the incomplete research evidence, we suggest the proposed framework may support future research design and health care interventions for people with ME/CFS

New structural insights into densely assembled reduced graphene oxide membranes

Densely assembled graphene-based membranes have attracted substantial interest for their widespread applications, such as compact capacitive energy storage, ion/molecular separation, gas barrier films, and flexible electronics. However, the multiscale structure of densely packed graphene membranes remains ambiguously understood. This article combines X-ray and light scattering techniques as well as dynamic electrosorption analysis to uncover the stacking structure of the densely stacked reduced graphene oxide (rGO) membranes. The membranes are produced by reducing graphene oxide (GO) membranes with hydrazine, during which the colloidal interactions between GO sheets are modulated by the electrolyte solution. In contrast to the common notion that direct reduction of densely assembled GO sheets in parallel tends to result in significant “graphitization”, this article unexpectedly discovers that the resultant densely packed rGO membrane can still retain the interconnected network nanochannels and show good capacitive performances. This inspires the development of a hierarchical structural model to describe the densely packed rGO membranes. This article further shows that the nanochannel network can be fine-tuned at the sub-nanometer level by tailoring the salt concentration and the reduction temperature to render exceptional volumetric capacitance and good rate performance for rGO membranes even with increased packing density

Atlas of modern dinoflagellate cyst distributions in the Black Sea Corridor: From Aegean to Aral Seas, including Marmara, Black, Azov and Caspian Seas

We present the first comprehensive taxonomic and environmental study of dinoflagellate cysts in 185 surface sediment samples from the Black Sea Corridor (BSC) which is a series of marine basins extending from the Aegean to the Aral Seas (including Marmara, Black, Azov and Caspian Seas). For decades, these low-salinity, semi-enclosed or endorheic basins have experienced large-scale changes because of intensive agriculture and industrialisation, with consequent eutrophication and increased algal blooms. The BSC atlas data provide a baseline for improved understanding of linkages between surface water conditions and dinoflagellate cyst (dinocyst) distribution, diversity and morphological variations. By cross-reference to dinocyst occurrences in sediment cores with radiocarbon ages covering the past c. 11,700 years, the history of recent biodiversity changes can be evaluated. The seabed cyst samples integrate seasonal and multi-year data which are not usually captured by plankton samples, and the cyst composition can point to presence of previously unrecorded motile dinoflagellate species in the BSC. Results show the presence of at least 71 dinocyst taxa of which 36% can be related to motile stages recorded in the plankton. Comparison with sediment core records shows that five new taxa appear to have entered or re-entered the region over the past century. Statistical analysis of the atlas data reveals the presence of four ecological assemblages which are primarily correlated with seasonal and annual surface water salinity and temperature; correlation with phosphate, nitrate and silicate nutrients, chlorophyll-a and bottom water oxygen is less clear but may be important for some taxa. Biodiversity indices reveal strong west − east biogeographical differences among the basins that reflect the different histories of Mediterranean versus Ponto-Caspian connections. The atlas data provide a standardised taxonomy and regional database for interpreting downcore cyst variations in terms of quantitative oceanographic changes. The atlas also provides a baseline for monitoring further changes in the BSC dinocysts that may accompany the accelerating development of the region

Magnetic resonance imaging quantification of fasted state colonic liquid pockets in healthy humans

The rate and extent of drug dissolution and absorption from solid oral dosage forms is highly dependent on the volume of liquid in the gastrointestinal tract (GIT). However, little is known about the time course of GIT liquid volumes after drinking a glass of water (8 oz), particularly in the colon, which is a targeted site for both locally and systemically acting drug products. Previous magnetic resonance imaging (MRI) studies offered novel insights on GIT liquid distribution in fasted humans in the stomach and small intestine, and showed that freely mobile liquid in the intestine collects in fairly distinct regions or “pockets”. Based on this previous pilot data, we hypothesized that (1) it is possible to quantify the time course of the volume and number of liquid pockets in the undisturbed colon of fasted healthy humans following ingestion of 240 mL, using noninvasive MRI methods; (2) the amount of freely mobile water in the fasted human colon is of the order of only a few milliliters. Twelve healthy volunteers fasted overnight and underwent fasted abdominal MRI scans before drinking 240 mL (∼8 fluid ounces) of water. After ingesting the water they were scanned at frequent intervals for 2 h. The images were processed to quantify freely mobile water in the total and regional colon: ascending, transverse, and descending. The fasted colon contained (mean ± SEM) 11 ± 5 pockets of resting liquid with a total volume of 2 ± 1 mL (average). The colonic fluid peaked at 7 ± 4 mL 30 min after the water drink. This peak fluid was distributed in 17 ± 7 separate liquid pockets in the colon. The regional analysis showed that pockets of free fluid were found primarily in the ascending colon. The interindividual variability was very high; the subjects showed a range of number of colonic fluid pockets from 0 to 89 and total colonic freely mobile fluid volume from 0 to 49 mL. This is the first study measuring the time course of the number, regional location, and volume of pockets of freely mobile liquid in the undisturbed colon of fasted humans after ingestion of a glass of water. Novel insights into the colonic fluid environment will be particularly relevant to improve our understanding and design of the in vivo performance of controlled release formulations targeted to the colon. The in vivo quantitative information presented here can be input into physiologically based mechanistic models of dissolution and absorption, and can be used in the design and set up of novel in vitro performance tools predictive of the in vivo environment

Ancient DNA derived from alkenone-biosynthesizing haptophytes and other algae in Holocene sediments from the Black Sea

Author Posting. © American Geophysical Union, 2006. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Paleoceanography 21 (2006): PA1005, doi:10.1029/2005PA001188.Holocene sea surface temperatures (SST) of the Black Sea have been reconstructed using sedimentary C37 unsaturated alkenones assumed to be derived from the coccolithophorid haptophyte Emiliania huxleyi, whose fossil coccoliths are an important constituent of the unit I sediments. However, alkenones can also be biosynthesized by haptophyte species that do not produce microscopic recognizable coccoliths. A species-specific identification of haptophytes is important in such U 37 K′-based past SST reconstructions since different species have different alkenone-SST calibrations. We showed that 18S rDNA of E. huxleyi made up only a very small percentage (less than 0.8%) of the total eukaryotic 18S rDNA within the up to 3600-year-old fossil record obtained from the depocenter (>2000 m) of the Black Sea. The predominant fossil 18S rDNA was derived from dinoflagellates (Gymnodinium spp.), which are predominant members of the summer phytoplankton bloom in the modern Black Sea. Using a polymerase chain reaction/denaturing gradient gel electrophoresis method selective for haptophytes, we recovered substantial numbers of a preserved 458-base-pair (bp)-long 18S rDNA fragment of E. huxleyi from the Holocene Black Sea sediments. Additional fossil haptophyte sequences were not detected, indicating that the E. huxleyi alkenone-SST calibration can be applied for at least the last ∼3600 years. The ancient E. huxleyi DNA was well protected against degradation since the DNA/alkenone ratio did not significantly decrease throughout the whole sediment core and 20% of ∼2700-year-old fossil E. huxleyi DNA was still up to 23,000 base pairs long. We showed that fossil DNA offers great potential to study the Holocene paleoecology and paleoenvironment of anoxic deep-sea settings in unprecedented detail.This work was supported by a grant from the Netherlands Organization for Scientific Research (NWO) (Open Competition Program 813.13.001 to M.J.L.C.) and NSF grant OCE0117824 to S.G.W., which we greatly appreciate