Size distribution and waiting times for the avalanches of the Cell Network Model of Fracture

The Cell Network Model is a fracture model recently introduced that resembles the microscopical structure and drying process of the parenchymatous tissue of the Bamboo Guadua angustifolia. The model exhibits a power-law distribution of avalanche sizes, with exponent -3.0 when the breaking thresholds are randomly distributed with uniform probability density. Hereby we show that the same exponent also holds when the breaking thresholds obey a broad set of Weibull distributions, and that the humidity decrements between successive avalanches (the equivalent to waiting times for this model) follow in all cases an exponential distribution. Moreover, the fraction of remaining junctures shows an exponential decay in time. In addition, introducing partial breakings and cumulative damages induces a crossover behavior between two power-laws in the avalanche size histograms. This results support the idea that the Cell Network Model may be in the same universality class as the Random Fuse Model

Hall-effect and resistivity measurements in CdTe and ZnTe at high pressure: Electronic structure of impurities in the zincblende phase and the semi-metallic or metallic character of the high-pressure phases

We carried out high-pressure resistivity and Hall-effect measurements in single crystals of CdTe and ZnTe up to 12 GPa. Slight changes of transport parameters in the zincblende phase of CdTe are consitent with the shallow character of donor impurities. Drastic changes in all the transport parameters of CdTe were found around 4 GPa, i.e. close to the onset of the cinnabar to rock-salt transition. In particular, the carrier concentration increases by more than five orders of magnitude. Additionally, an abrupt decrease of the resistivity was detected around 10 GPa. These results are discussed in comparison with optical, thermoelectric, and x-ray diffraction experiments. The metallic character of the Cmcm phase of CdTe is confirmed and a semi-metallic character is determined for the rock-salt phase. In zincblende ZnTe, the increase of the hole concentration by more than two orders of magnitude is proposed to be due to a deep-to-shallow transformation of the acceptor levels. Between 9 and 11 GPa, transport parameters are consistent with the semiconducting character of cinnabar ZnTe. A two orders of magnitude decrease of the resistivity and a carrier-type inversion occurs at 11 GPa, in agreement with the onset of the transition to the Cmcm phase of ZnTe. A metallic character for this phase is deduced.Comment: 20 pages, 4 figure

Optimal design of steel¿concrete composite bridge based on a transfer function discrete swarm intelligence algorithm

[EN] Bridge optimization can be complex because of the large number of variables involved in the problem. In this paper, two box-girder steel¿concrete composite bridge single objective optimizations have been carried out considering cost and CO¿ emissions as objective functions. Taking CO¿ emissions as an objective function allows adding sustainable criteria to compare the results with cost. SAMO2, SCA, and Jaya metaheuristics have been applied to reach this goal. Transfer functions have been implemented to fit SCA and Jaya to the discontinuous nature of the bridge optimization problem. Furthermore, a Design of Experiments has been conducted to tune the algorithm and set its parameters. Consequently, it has been observed that SCA shows similar values for objective cost function as SAMO2 but improves computational time by 18% while also getting lower values for the objective function result deviation. From a cost and CO¿ optimization analysis, it has been observed that a reduction of 2.51 kg CO¿ is obtained by each euro reduced using metaheuristic techniques. Moreover, for both optimization objectives, it is observed that adding cells to bridge cross-sections improves not only the section behavior but also the optimization results. Finally, it is observed that the proposed design of double composite action in the supports allows this study to remove continuous longitudinal stiffeners in the bottom flange.Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research has been made possible thanks to funding received from the following research projects: Grant PID2020-117056RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe", Grant FPU-18/01592 funded by MCIN/AEI/10.13039/501100011033 and by "ESF invests in your future" and Grant CONICYT/FONDECYT/INICIACION/11180056.Martínez-Muñoz, D.; García, J.; Martí Albiñana, JV.; Yepes, V. (2022). Optimal design of steel¿concrete composite bridge based on a transfer function discrete swarm intelligence algorithm. Structural and Multidisciplinary Optimization. 65(11):1-25. https://doi.org/10.1007/s00158-022-03393-9125651

Hybrid Swarm Intelligence Optimization Methods for Low-Embodied Energy Steel-Concrete Composite Bridges

[EN] Bridge optimization is a significant challenge, given the huge number of possible configurations of the problem. Embodied energy and cost were taken as objective functions for a box-girder steel¿concrete optimization problem considering both as single-objective. Embodied energy was chosen as a sustainable criterion to compare the results with cost. The stochastic global search TAMO algorithm, the swarm intelligence cuckoo search (CS), and sine cosine algorithms (SCA) were used to achieve this goal. To allow the SCA and SC techniques to solve the discrete bridge optimization problem, the discretization technique applying the k-means clustering technique was used. As a result, SC was found to produce objective energy function values comparable to TAMO while reducing the computation time by 25.79%. In addition, the cost optimization and embodied energy analysis revealed that each euro saved using metaheuristic methodologies decreased the energy consumption for this optimization problem by 0.584 kW·h. Additionally, by including cells in the upper and lower parts of the webs, the behavior of the section was improved, as were the optimization outcomes for the two optimization objectives. This study concludes that double composite action design on supports makes the continuous longitudinal stiffeners in the bottom flange unnecessary.The author gratefully acknowledge the fundings received by: Grant PID2020- 117056RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ¿ERDF A way of making Europe¿. Grant FPU-18/01592 funded by MCIN/AEI/10.13039/501100011033 and by ¿ESF invests in your future¿ and Grant CONICYT/FONDECYT/INICIACION/11180056Martínez-Muñoz, D.; García, J.; Martí Albiñana, JV.; Yepes, V. (2022). Hybrid Swarm Intelligence Optimization Methods for Low-Embodied Energy Steel-Concrete Composite Bridges. Mathematics. 11(1):1-21. https://doi.org/10.3390/math1101014012111

Analysis Of Energy Production With Different Photovoltaic Technologies In The Colombian Geography

This research has analyzed the photovoltaic technologies, Polycrystalline silicon, Monocrystalline Silicon, GIS, Cadmium Tellurium and Amorphous Silicon; in eight cities of the Colombian territory, in order to obtain a clear idea of what is the most appropriate for each city or region studied. PVsyst simulation software has been used to study in detail each photovoltaic technology, for an installed capacity of 100kW knowing the specific data of losses by temperature, mismatch, efficiency, wiring, angle inclination of the arrangement, among other

New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses

This work was financially supported by CTQ2017-85685-R, CTQ2017-85454-C2-1-P, and CTQ2017-86125-P (MICIU/AEI/ERDF). J.M.P. and J.D.V. thank UEQ for funding. J.V.P. is supported by an FPU fellowship (FPU17/04749). D.A. was supported by the Italian Cystic Fibrosis Foundation (grant FFC#3/2019) and by Fondazione Cassa Rurale Trento Rovereto (ref 2018.256). The authors acknowledge the Universidad of Granada (Spain) cell culture and microscopy central facilities (CIC-UGR).A new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via sitedirected mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitude (kd = 106 ± 6 mM) at physiological pH. In addition, cis−trans isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations.MICIU/AEI/ERDF CTQ2017-85685-R CTQ2017-85454-C2-1-P CTQ2017-86125-PUEQSpanish Government FPU17/04749Ministry of Health, ItalyItalian Cystic Fibrosis Research Foundation 3/2019Fondazione Cassa Rurale Trento Rovereto 2018.25

Combinatorial gene therapy renders increased survival in cirrhotic rats

<p>Abstract</p> <p>Background</p> <p>Liver fibrosis ranks as the second cause of death in México's productive-age population. This pathology is characterized by acummulation of fibrillar proteins in hepatic parenchyma causing synthetic and metabolic disfunction. Remotion of excessive fibrous proteins might result in benefit for subjects increasing survival index. The goal of this work was to find whether the already known therapeutical effect of human urokinase Plasminogen Activator and human Matrix Metalloprotease 8 extends survival index in cirrhotic animals.</p> <p>Methods</p> <p>Wistar rats (80 g) underwent chronic intoxication with CCl₄: mineral oil for 8 weeks. Cirrhotic animals were injected with a combined dose of Ad-delta-huPA plus Ad-MMP8 (3 × 10¹¹and 1.5 × 10¹¹vp/Kg, respectively) or with Ad-beta-Gal (4.5 × 10¹¹) and were killed after 2, 4, 6, 8 and 10 days. Then, liver and serum were collected. An additional set of cirrhotic animals injected with combined gene therapy was also monitored for their probability of survival.</p> <p>Results</p> <p>Only the cirrhotic animals treated with therapeutical genes (Ad-delta-huPA+Ad-MMP-8) showed improvement in liver fibrosis. These results correlated with hydroxyproline determinations. A significant decrement in alpha-SMA and TGF-beta1 gene expression was also observed. Cirrhotic rats treated with Ad-delta-huPA plus Ad-MMP8 had a higher probability of survival at 60 days with respect to Ad-beta-Gal-injected animals.</p> <p>Conclusion</p> <p>A single administration of Ad-delta-huPA plus Ad-MMP-8 is efficient to induce fibrosis regression and increase survival in experimental liver fibrosis.</p

Calcium homeostasis plays important roles in the internalisation and activities of the small synthetic antifungal peptide PAF26

Fungal diseases are responsible for the deaths of over 1.5 million people worldwide annually. Antifungal peptides represent a useful source of antifungals with novel mechanisms-of-action, and potentially provide new methods of overcoming resistance. Here we investigate the mode-of-action of the small, rationally designed synthetic antifungal peptide PAF26 using the model fungus Neurospora crassa. Here we show that the cell killing activity of PAF26 is dependent on extracellular Ca2+ and the presence of fully functioning fungal Ca2+ homeostatic/signalling machinery. In a screen of mutants with deletions in Ca2+-signalling machinery, we identified three mutants more tolerant to PAF26. The Ca2+ ATPase NCA-2 was found to be involved in the initial interaction of PAF26 with the cell envelope. The vacuolar Ca2+ channel YVC-1 was shown to be essential for its accumulation and concentration within the vacuolar system. The Ca2+ channel CCH-1 was found to be required to prevent the translocation of PAF26 across the plasma membrane. In the wild type, Ca2+ removal from the medium resulted in the peptide remaining trapped in small vesicles as in the Δyvc-1 mutant. It is therefore apparent that cell killing by PAF26 is complex and unusually dependent on extracellular Ca2+ and components of the Ca2+-regulatory machinery

The effects of death and post-mortem cold ischemia on human tissue transcriptomes

Post-mortem tissues samples are a key resource for investigating patterns of gene expression. However, the processes triggered by death and the post-mortem interval (PMI) can significantly alter physiologically normal RNA levels. We investigate the impact of PMI on gene expression using data from multiple tissues of post-mortem donors obtained from the GTEx project. We find that many genes change expression over relatively short PMIs in a tissue-specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates. By comparing ante- and post-mortem blood samples, we identify the cascade of transcriptional events triggered by death of the organism. These events do not appear to simply reflect stochastic variation resulting from mRNA degradation, but active and ongoing regulation of transcription. Finally, we develop a model to predict the time since death from the analysis of the transcriptome of a few readily accessible tissues.Peer ReviewedPostprint (published version

Estudio de pre-factibilidad técnica y económica de la implementación de una biorrefinería para la conversión de residuos de cáscara de naranja

Orange peel is an abundant organic waste in Ecuador that can be used and transformed into products of high added value. Therefore, this work analyzes the technical-economic pre-feasibility of a biorefinery from orange peel to obtain essential oil, pectin and / or bioethanol. First, several scenarios were compared around the combination of products to be obtained, and it was established that the most convenient one would be obtaining essential oil and pectin, with an annual production of 8.7 and 44.4 tons, respectively. Next, a process was designed for this biorefinery that consists of 3 sections: the pretreatment of raw material, extraction of the oil, and the extraction of pectin. Material and energy balances of the process were carried out, and later, the equipment was selected and sized according to specific methodologies. The process considered the recovery and recirculation of ethanol used in the pectin extraction section to reduce production costs. Finally, an economic analysis was carried out based on the theoretical cost estimates and a catalog cost analysis. It was found that the project is profitable, and that the payback time for the investment would be between 5 and 6 years. Therefore, the implementation of the biorefinery would generate a positive economic, environmental and social impact in the country.La cáscara de naranja es un residuo orgánico abundante en el Ecuador que puede ser aprovechado y transformado en productos de alto valor agregado. Por ello, el presente artículo analiza la pre-factibilidad técnico-económica de una biorrefinería a partir de cáscara de naranja para la obtención de aceite esencial, pectina y/o bioetanol. Primero, se compararon varios escenarios alrededor de la combinación de productos a ser obtenidos, y se estableció que el más conveniente sería la obtención de aceite esencial y pectina, con una producción anual de 8,7 y 44,4 toneladas, respectivamente. A continuación, se diseñó un proceso para esta biorrefinería que consiste en 3 secciones: el pretratamiento de la materia prima, la extracción del aceite, y la extracción de la pectina. Se realizaron los balances de materia y energía del proceso, y posteriormente, se seleccionaron y dimensionaron los equipos de acuerdo a metodologías específicas. El proceso consideró la recuperación y recirculación de etanol empleado en la sección de extracción de pectina para reducir los costos de producción. Finalmente, se realizó un análisis económico a partir de las estimaciones de costos teóricos y un análisis de costos de catálogo. Se encontró que el proyecto es rentable y que el tiempo de recuperación de la inversión estaría entre 5 y 6 años. Por lo tanto, la implementación de la biorrefinería generaría un impacto positivo a nivel económico, ambiental y social en el país