Effect of plant seeds protein extract on the Sunn pest, Eurygaste integriceps Puton, growth and development and its gut serine protease activity

The sunn pest Eurygaster integriceps Puton (Hemiptera: Scutelleridae) is a major pest of wheat and barley in wide areas of the world that cause 100% crop loss when no control measures are taken. The aim of this study was to investigate the effect of the seed proteinous extract of different plant species including Chickpea Cicer arietinum (Fabaceae), bean Phaseolus vulgaris var. naz (Fabaceae), triticale Triticosecale wittmack (Poaceae), Celosia argentea (Amaranthaceae) in artificial diet against the Sunn pest growth and development as well as gut serine proteinase such as trypsin and chymotrypsin activities. The obtained results showed that protease inhibitors present in the seed extract affected nymphal development, adult weight and survivability to some extent. Mean developmental time of third and fourth instar nymphs were not significantly affected by the presence of seed extracts. However, developmental time of the fifth instar nymph was affected by seeds proteinous extracts. Bean proteinous extract increased developmental time significantly by almost two days followed by Chickpea that increased developmental time by one day (P < 0.01). Amaranthus seed proteinous extract caused slight increase in development time. Triticale extract did not affect nymph growth and development and there were no significant differences between developmental time of triticale extract and control. Azocaseinolytic activity of gut extract of E. integriceps was affected greatly by seed proteinous extracts. There were significant differences in general protease activity between control and all treatments. General protease activity dropped significantly low when treated with bean and cowpea extracts (P< 0.01). Almost the same trend was observed when trypsin and chymotrypsin activities were measured using BApNA and SAAPFpNA as substrates, respectively. It is concluded that seed extracts from non host plants of the Sunn pest caused significant reduction of general and specific protease activity in vitro.Key words: Sunn pest, growth, development, seed extracts, protease activity

Complications and carcinogenic effects of mustard gas - A systematic review and meta-analysis in Iran

Background: Catastrophic effects of mustard gas as a chemical warfare agent have always been a major problem for those exposed to this agent. In this meta-analysis it was tried to evaluate carcinogenesis, ocular, cutaneous and respiratory complications of mustard gas exposure among Iranians who had been exposed to this agent during the Iran-Iraq war. Materials and Methods: In this meta-analysis, the required data were collected using keywords "mustard gas", "sulfur mustard", "cancer", "neoplasm", "respiratory complications", "ocular complications", "lung disease", "chronic complication", "eye", "skin", "cutaneous complication", "carcinogenesis" and their combination with keywords "Iran", "Iranian", "prevalence", "mortality" and their Farsi equivalent terms from the databases of SID, Iranmedex, Magiran, Pubmed, Science Direct, Google Search engine, Gray Literature and Reference of References. To determine the prevalence of each complication and perform meta-analysis, CMA: 2 (Comprehensive Meta-Analysis) software with a randomized model was used. Results: Of the 542 articles found, 7 national articles, consistent with the aims of this study were selected. Metaanalysis of seven papers revealed that cancer risk, especially cancer of the respiratory system was elevated, so that the relative risk (RR) of cancer role of mustard gas was inconsistent from 2/1 to 4 in this survey. Also prevalence of delayed skin disorders due to sulfur mustard was 94.6, pulmonary complications 94.5 and ocular complications 89.9. The incidence of various cancers in victims exposed to mustard gas was 1.7 worldwide where the rate was 2.2 in Iranian victims of the Iraq-Iran war. Conclusions: Based on present study the prevalence of delayed mustard gas related cutaneous, pulmonary and ocular complications is above 90 and risk of carcinogenesis is higher in comparison to worldwide statistics. This may suggest need for long-term and persistent follow-up and rehabilitation procedures es for populations exposed to this agent

Bi-allelic GAD1 variants cause a neonatal onset syndromic developmental and epileptic encephalopathy.

Developmental and epileptic encephalopathies are a heterogeneous group of early-onset epilepsy syndromes dramatically impairing neurodevelopment. Modern genomic technologies have revealed a number of monogenic origins and opened the door to therapeutic hopes. Here we describe a new syndromic developmental and epileptic encephalopathy caused by bi-allelic loss-of-function variants in GAD1, as presented by 11 patients from six independent consanguineous families. Seizure onset occurred in the first 2 months of life in all patients. All 10 patients, from whom early disease history was available, presented with seizure onset in the first month of life, mainly consisting of epileptic spasms or myoclonic seizures. Early EEG showed suppression-burst or pattern of burst attenuation or hypsarrhythmia if only recorded in the post-neonatal period. Eight patients had joint contractures and/or pes equinovarus. Seven patients presented a cleft palate and two also had an omphalocele, reproducing the phenotype of the knockout Gad1-/- mouse model. Four patients died before 4 years of age. GAD1 encodes the glutamate decarboxylase enzyme GAD67, a critical actor of the γ-aminobutyric acid (GABA) metabolism as it catalyses the decarboxylation of glutamic acid to form GABA. Our findings evoke a novel syndrome related to GAD67 deficiency, characterized by the unique association of developmental and epileptic encephalopathies, cleft palate, joint contractures and/or omphalocele

The nonlinear analysis of an innovative slit reinforced concrete water tower in seismic regions

Water towers are widely used in our society as one of water distribution facilities within water network systems. In the event of a severe earthquake, however, a single plastic hinge that occurs in a water tower could cause its total collapse before nonlinear resources of the rest of the tower remains fully utilised. This research presents an innovative technique for the assembly of a water tower using the slits in its reinforced concrete shaft for the purpose of mitigating the seismic response. Slit shafts were designed to have four slits at 90 degree intervals along the full height of the shafts. The shaft parts were connected to each other at the bottom, top and every five meters with coupling beams. The slit width was used as a variable in this study which varied between 50 mm and 2000 mm. The nonlinear seismic performance of the proposed slit towers was analysed by means of a finite element approach with respect to soil types defined in Eurocode 8 and seismic behaviour were compared to the solid water tower. A detailed observation of the compression and tension stress distributions with respect to the slit width was performed. The obtained analytical results revealed that slit width in the reinforced concrete tower affect the failure mode and stiffness of a water tower significantly. With an appropriate design, the conversion of a solid water tower into a slit tower can significantly increase its ductility under seismic action without significantly compromising its bearing capacity. The results showed that contours of tension and compression stress intensity in shafts, which could lead to a failure of water towers, highly depended on the slit width. In the solid water tower, the stress concentration dominated at the base of the shaft, however in the narrow slit water towers the stresses were equally distributed along the height of the shafts. Also, the stresses were mostly concentrated at the top of the shafts in the wide slit water towers. Conclusively, the results provided useful information regarding the compression stress distribution along the slit shafts in the water towers which can be used in obtaining an optimum slit shaft design for different soil types

Non-conventional therapeutics for oral infections

© 2015 Robert P Allaker and CW Ian Douglas. As our knowledge of host-microbial interactions within the oral cavity increases, future treatments are likely to be more targeted. For example, efforts to target a single species or key virulence factors that they produce, while maintaining the natural balance of the resident oral microbiota that acts to modulate the host immune response would be an advantage. Targeted approaches may be directed at the blackpigmented anaerobes, Porphyromonas gingivalis and Prevotella intermedia, associated with periodontitis. Such pigments provide an opportunity for targeted phototherapy with high-intensity monochromatic light. Functional inhibition approaches, including the use of enzyme inhibitors, are also being explored to control periodontitis. More general disruption of dental plaque through the use of enzymes and detergents, alone and in combination, shows much promise. The use of probiotics and prebiotics to improve gastrointestinal health has now led to an interest in using these approaches to control oral disease. More recently the potential of antimicrobial peptides and nanotechnology, through the application of nanoparticles with biocidal, antiadhesive and delivery capabilities, has been explored. The aim of this review is to consider the current status as regards non-conventional treatment approaches for oral infections with particular emphasis on the plaque-related diseases

Prevalence of antibodies to a new histo-blood system: the FORS system

In 1987, three unrelated English families were reported with a putative blood subgroup called Apae. Swedish researchers later found evidence leading to abolishment of the Apae subgroup and establishment instead of the FORS blood group system (System 31 - ISBT, 2012). It is important to know the prevalence of antibodies in order to make the best decisions in transfusion medicine. Cells expressing the Forssman saccharide, such as sheep erythrocytes, are needed to detect the anti-Forssman antibody. The aim of this study was to define the prevalence of human anti-Forssman antibody.info:eu-repo/semantics/publishedVersio