Hybrids between Pseudoplatystoma corruscans and P. reticulatum (Siluriformes: Pimelodidae) previously reported in the Upper Paraná River are likely escapes from aquaculture farms: evidence from microsatellite markers

ABSTRACT The production of hybrids of the 'pintado', Pseudoplatystoma corruscans (Spix & Agassiz, 1829) and 'cachara', Pseudoplatystoma reticulatum (Eigenmann & Eigenmann, 1889) in captivity has generated many concerns about the possibility of introduction of farmed hybrids into natural environments. In the last decade, hybrids between these species, known as 'pintachara' or 'cachapinta', were reported from different regions of the Upper Paraná River basin. Prospection of these hybrids is important in order to orient conservation programs for the species involved. Knowledge of the presence of these hybrids will direct conservation strategies towards prevention and/or mitigation of the effects of cross breeding in natural populations of P. corruscans (the native species of the genus) and farmed hybrids. In this study, surveyed the larval population using molecular tools to detect the presence and assess the origin (natural hybridization or escapes from fish farms) of hybrids in natural water bodies. Nine microsatellite markers were used to detect signals of hybridization and introgression of P. reticulatum in larvae and adults of P. corruscans in Upper Paraná River basin, between Itaipu Dam and Porto Primavera Dam. The specimens were sampled in the Upper Paraná channel and in tributaries where hybrids had been detected in the past, during two reproductive seasons. Despite of that, no sign of hybridization and introgression was found in the 171 larvae and 75 adults sampled, suggesting that the specimens detected in previous studies had originated from escapes of aquaculture farms

1RXS J214303.7+065419/RBS 1774: A New Isolated Neutron Star Candidate

We report on the identification of a new possible Isolated Neutron Star candidate in archival ROSAT observations. The source 1RXS J214303.7+065419, listed in the ROSAT Bright Survey as RBS 1774, is very soft, exhibits a thermal spectrum well fitted by a blackbody at ${T}\sim 90$ eV and has a low column density, ${N_H}\sim 5\times 10^{20}$ ${cm}^{-2}$. Catalogue searches revealed no known sources in other energy bands close to the X-ray position of RBS 1774. Follow-up optical observations with NTT showed no peculiar object within the X-ray error circle. The absence of any plausible optical counterpart down to ${m_R}\sim 23$ results in an X-ray to optical flux ratio in excess of 1000.Comment: LaTeX (A&A style files), 5 pages, 3 figures. Accepted for publication in Astronomy and Astrophysics Letters. Minor correction

Conversion of the Native N-Terminal Domain of TDP-43 into a Monomeric Alternative Fold with Lower Aggregation Propensity.

TAR DNA-binding protein 43 (TDP-43) forms intraneuronal cytoplasmic inclusions associated with amyotrophic lateral sclerosis and ubiquitin-positive frontotemporal lobar degeneration. Its N-terminal domain (NTD) can dimerise/oligomerise with the head-to-tail arrangement, which is essential for function but also favours liquid-liquid phase separation and inclusion formation of full-length TDP-43. Using various biophysical approaches, we identified an alternative conformational state of NTD in the presence of Sulfobetaine 3-10 (SB3-10), with higher content of α-helical structure and tryptophan solvent exposure. NMR shows a highly mobile structure, with partially folded regions and β-sheet content decrease, with a concomitant increase of α-helical structure. It is monomeric and reverts to native oligomeric NTD upon SB3-10 dilution. The equilibrium GdnHCl-induced denaturation shows a cooperative folding and a somewhat lower conformational stability. When the aggregation processes were compared with and without pre-incubation with SB3-10, but at the identical final SB3-10 concentration, a slower aggregation was found in the former case, despite the reversible attainment of the native conformation in both cases. This was attributed to protein monomerization and oligomeric seeds disruption by the conditions promoting the alternative conformation. Overall, the results show a high plasticity of TDP-43 NTD and identify strategies to monomerise TDP-43 NTD for methodological and biomedical applications

The enigmatic pair of dwarf galaxies Leo IV and Leo V: coincidence or common origin?

We have obtained deep photometry in two 1x1 degree fields covering the close pair of dwarf spheroidal galaxies (dSph) Leo IV and Leo V and part of the area in between. We find that both systems are significantly larger than indicated by previous measurements based on shallower data and also significantly elongated. With half-light radii of r_h=4'.6 +- 0'.8 (206 +- 36 pc) and r_h=2'.6 +- 0'.6 (133 +- 31 pc), respectively, they are now well within the physical size bracket of typical Milky Way dSph satellites. Their ellipticities of epsilon ~0.5 are shared by many faint (M_V>-8) Milky Way dSphs. The large spatial extent of our survey allows us to search for extra-tidal features with unprecedented sensitivity. The spatial distribution of candidate red giant branch and horizontal branch stars is found to be non-uniform at the ~3 sigma level. This substructure is aligned along the direction connecting the two systems, indicative of a possible `bridge' of extra-tidal material. Fitting the stellar distribution with a linear Gaussian model yields a significance of 4 sigma for this overdensity, a most likely FWHM of ~16 arcmin and a central surface brightness of ~32 mag arcsec^{-2}. We investigate different scenarios to explain the close proximity of Leo IV and Leo V and the possible tidal bridge between them. Orbit calculations demonstrate that they are unlikely to be remnants of a single disrupted progenitor, while a comparison with cosmological simulations shows that a chance collision between unrelated subhalos is negligibly small. Leo IV and Leo V could, however, be a bound `tumbling pair' if their combined mass exceeds 8 +- 4 x 10^9 M_sun. The scenario of an internally interacting pair appears to be the most viable explanation for this close celestial companionship. (abridged)Comment: 9 pages, 8 figures, small number of minor textual changes, accepted for publication in Astrophysical Journa

Resolving the Galactic X-ray background

We use Chandra deep observations of the Galactic Center (GC) region to improve the constraints on the unresolved fraction of the Galactic X-ray background (also known as the Galactic ridge X-ray emission). We emphasize the importance of correcting the measured source counts at low fluxes for bias associated with Poisson noise. We find that at distances of 2'-4' from Sgr A* at least ~40% of the total X-ray emission in the energy band 4-8 keV originates from point sources with luminosities L(2-10 keV)> 10^{31} erg/sec. From a comparison of the source number-flux function in the GC region with the known luminosity function of faint X-ray sources in the Solar vicinity, we infer that Chandra has already resolved a large fraction of the cumulative contribution of cataclysmic variables to the total X-ray flux from the GC region. This comparison further indicates that most of the yet unresolved ~60% of the X-ray flux from the GC region is likely produced by weak cataclysmic variables and coronally active stars with L(2-10 keV)<10^{31} erg/sec. We conclude that the bulk of the Galactic X-ray background is produced by discrete sources.Comment: Submitted to A&

Blank Field Sources in the ROSAT HRI Brera Multiscale Wavelet catalog

The search for Blank Field Sources (BFS), i.e. X-ray sources without optical counterparts, paves the way to the identification of unusual objects in the X-ray sky. Here we present four BFS detected in the Brera Multiscale Wavelet catalog of ROSAT HRI observations. This sample has been selected on the basis of source brightness, distance from possible counterparts at other wavelengths, point-like shape and good estimate of the X-ray flux (f_X). The observed f_X and the limiting magnitude of the optical catalogs fix a lower limit for our BFS on f_X/f_opt~40. This value puts them well beyond 90% threshold for usual source classes once HRI energy band and proper spectral shape are taken into account, leaving room for speculation on their nature. Three BFS show also evidence of a transient behaviour.Comment: 9 pages, 5 figures, accepted for publication on A&

IGR J18483-0311: an accreting X-ray pulsar observed by INTEGRAL

IGR J18483-0311 is a poorly known transient hard X-ray source discovered by INTEGRAL during observations of the Galactic Center region performed between 23--28 April 2003. Aims: To detect new outbursts from IGR J18483-0311 using INTEGRAL and archival Swift XRT observations and finally to characterize the nature of this source using the optical/near-infrared (NIR) information available through catalogue searches. Results: We report on 5 newly discovered outbursts from IGR J18483-0311 detected by INTEGRAL.For two of them it was possible to constrain a duration of the order of a few days. The strongest outburst reached a peak flux of 120 mCrab (20--100 keV): its broad band JEM--X/ISGRI spectrum (3--50 keV) is best fitted by an absorbed cutoff power law with photon index=1.4+/-0.3, cutoff energy of ~22 keV and Nh ~9x10^22 cm^-2. Timing analysis of INTEGRAL data allowed us to identify periodicities of 18.52 days and 21.0526 seconds which are likely the orbital period of the system and the spin period of the X-ray pulsar respectively. Swift XRT observations of IGR J18483$-$0311 provided a very accurate source position which strongly indicates a highly reddened star in the USNO--B1.0 and 2MASS catalogues as its possible optical/NIR counterpart. Conclusions: The X-ray spectral shape, the periods of 18.52 days and 21.0526 seconds, the high intrinsic absorption, the location in the direction of the Scutum spiral arm and the highly reddened optical object as possible counterpart, all favour the hypothesis that IGR J18483-0311 is a HMXB with a neutron star as compact companion. The system is most likely a Be X-ray binary, but a Supergiant Fast X-ray Transient nature can not be entirely excluded.Comment: accepted for publication in A&A, 10 pages, 17 figures, 4 table

INTEGRAL hard X-ray spectra of the cosmic X-ray background and Galactic ridge emission

We derive the spectra of the cosmic X-ray background (CXB) and of the Galactic ridge X-ray emission (GRXE) in the ~20-200 keV range from the data of the IBIS instrument aboard the INTEGRAL satellite obtained during the four dedicated Earth-occultation observations of early 2006. We analyse the modulation of the IBIS/ISGRI detector counts induced by the passage of the Earth through the field of view of the instrument. Unlike previous studies, we do not fix the spectral shape of the various contributions, but model instead their spatial distribution and derive for each of them the expected modulation of the detector counts. The spectra of the diffuse emission components are obtained by fitting the normalizations of the model lightcurves to the observed modulation in different energy bins. The obtained CXB spectrum is consistent with the historic HEAO-1 results and falls slightly below the spectrum derived with Swift/BAT. A 10% higher normalization of the CXB cannot be completely excluded, but it would imply an unrealistically high albedo of the Earth. The derived spectrum of the GRXE confirms the presence of a minimum around 80 keV with improved statistics and yields an estimate of ~0.6 M_Sun for the average mass of white dwarfs in the Galaxy. The analysis also provides updated normalizations for the spectra of the Earth's albedo and the cosmic-ray induced atmospheric emission.Comment: 13 pages, 13 figures, minor changes to text, A&A in pres

Method to integrate management tools aiming organizational excellence

Paper aims The main objective of this article is to present and validate a method to integrate multiple management tools aiming for organizational excellence in medium and large enterprises. Originality There is a scarcity of methods to integrate management tools to support companies in their operations. This study aims to fill this gap, proposing a manner for companies to perform this integration. Research method The method was developed considering tools and concepts well-established in the literature, such as Lean Thinking, Six Sigma, Balanced ScoreCard, among other management tools. The mentioned method was validated through a survey with managers and directors who are experts in organizational strategies. They were carefully selected considering their professional background. Main findings The proposed method provides an alignment between strategy and execution, presenting a cyclical characteristic to be continuously reviewed method, considering market needs. The survey verified the adherence of the method and to conclude that it is a feasible alternative to reach organizational excellence. Although integration of management methods is fundamental for companies to reach organizational excellence status, this kind of guidelines is scarce in the literature. Implications for theory and practice This method can be used to increase companies’ performance and competitivity

Complex force history of a calving-generated glacial earthquake derived from broadband seismic inversion

The force applied to the Earth by the calving of two icebergs at Jakobshavn Isbrae, Greenland, has been quantified. The source force history was recovered by inversion of regional broadband seismograms without any a priori constraint on the source time function, in contrast with previous studies. For periods 10-100 s, the three-component force can be obtained from distant stations alone and is proportional to the closest station seismograms. This inversion makes it possible to quantify changes of the source force direction and amplitude as a function of time and frequency. A detailed comparison with a video of the event was used to identify four forces associated with collision, then bottom-out and top-out rotation of the first and second icebergs, and ice mélange motion. Only the two iceberg rotations were identified in previous studies. All four processes are found here to contribute to the force amplitude and variability. Such a complete time-frequency force history provides unique dynamical constraints for mechanical calving models.ERC. Grant Number: ERC-CG-2013-PE10-617472 ANR. Grant Number: ANR-11-BS01-0016 LANDQUAKES, CNCS‐UEFISCDI. Grant Number: PN-II-ID-PCE-2011-3-004