First Report of Asiatic Brown Rot (Monilinia polystroma) and Brown Rot (Monilinia fructicola) on Pears in Italy

Brown rot caused by Monilinia spp. is an important fruit postharvest decay causing severe losses in stone and pome fruits with a significant economic impact. In Italy, three Monilinia species (M. laxa, M. fructicola, and M. fructigena) are the causal agents of blossom and twig blight and brown fruit rot in stone fruit. M. polystroma has been observed on peaches in Italy (2) and has been reported in Czech Republic and Hungary (3), Poland (4), Serbia (5), and Switzerland (1) on pome fruits and apricots. In September 2013, stored var. Abate Fetel pears showing brown rot symptoms were observed in Emilia Romagna region. In 20% of the symptomatic pears, circular and brown to black decay spots were observed, covered by a large number of yellowish or buff-colored stromata, while decayed tissues remained firm, resembling M. polystroma symptoms. In another 13% of stored pears, the decayed tissues remained firm, and decay lesions were covered with numerous grayish pustules containing spores. Putative pathogens were isolated on potato dextrose agar (PDA) and incubated at 25°C in darkness for 5 days. The colonies grown on PDA were yellowish, with irregular black stromatal crusts at the edges of the colonies after 10 to 12 days of incubation. Some colonies developed, at their margins, sporogenous tissue slightly elevated above the colony surface that was buff/pale luteous (4). Conidia developing from such cultures were one celled, ovoid or limoniform, smooth and hyaline, measuring 12.2 to 20.4 × 8.4 to 12.3 µm when grown on V8 juice agar (V8) at 22°C, and matched the description of those for M. polystroma. Other colonies, which developed a gray mass of spores in concentric rings with the reverse side black, were morphologically identified as M. fructicola. The colony margins were smooth edged, and the conidia were one-celled, limoniform, hyaline, and measuring 12.1 to 17.4 × 8.1 to 11.2 µm on V8 at 22°C. Isolate identificaton was obtained using the universal primers for Monilinia spp. (3). Pathogenicity was confirmed using surface-sterilized mature var. Abate Fetel and William pears wounded with a sterile needle, and inoculated with 20 µl of an M. polystroma or M. fructicola conidial suspension (103 spores/ml). After 7 days of incubation at 20°C, typical symptoms of Asiatic brown rot or brown rot developed on both the wounds of all inoculated pears, while controls remained symptomless. Mean colony diameters measured after 7 days were 47.3 mm for Asiatic brown rot and 44.1 mm for brown rot, and there were no significant differences in colony diameter after 7 days between M. polystroma and M. fructicola (α < 0.05). After 14 days, yellowish exogenous stromata appeared on the surface of pears infected by M. polystroma, whereas numerous grayish pustules containing spores appeared on pears inoculated with M. fructicola. Control pears still remained symptomless. The fungus isolated from inoculated fruit exhibited the same morphological features as the original isolates, and PCR/sequencing analysis using primers ITS1 and ITS4 confirmed the results of the universal primers (3) (GenBank Accession Nos. GU067539.1 and HQ893748.1). Although the presence of M. polystroma and M. fructicola has been documented in Italy, this is the first time these two species were observed on Italian pears. This report suggests a broader impact since M. polystroma and M. fructicola have not been previously reported on pears in Europe. Because of the importance of pears in the Italian fruit industry, knowledge about the occurrence of new pathogens will facilitate the adoption of adequate control strategies to reduce postharvest losses

Photodegradation of polycyclic aromatic hydrocarbons in soils under a climate change base scenario

The photodegradation of polycyclic aromatic hydrocarbons (PAHs) in two typical Mediterranean soils, either coarse- or fine-textured, was here investigated. Soil samples, spiked with the 16 US EPA priority PAHs, were incubated in a climate chamber at stable conditions of temperature (20 degrees C) and light (9.6 W m(-2)) for 28 days, simulating a climate change base scenario. PAH concentrations in soils were analyzed throughout the experiment, and correlated with data obtained by means of Microtox (R) ecotoxicity test. Photodegradation was found to be dependent on exposure time, molecular weight of each hydrocarbon, and soil texture. Fine-textured soil was able to enhance sorption, being PAHs more photodegraded than in coarse-textured soil. According to the EC50 values reported by Microtox (R), a higher detoxification was observed in fine-textured soil, being correlated with the outcomes of the analytical study. Significant photodegradation rates were detected for a number of PAHs, namely phenanthrene, anthracene, benzo(a)pyrene, and indeno(123-cd)pyrene. Benzo(a)pyrene, commonly used as an indicator for PAH pollution, was completely removed after 7 days of light exposure. In addition to the PAH chemical analysis and the ecotoxicity tests, a hydrogen isotope analysis of benzo(a)pyrene was also carried out. The degradation of this specific compound was associated to a high enrichment in H-2, obtaining a maximum delta H-2 isotopic shift of +232 parts per thousand. This strong isotopic effect observed in benzo(a) pyrene suggests that compound-specific isotope analysis (CSIA) may be a powerful tool to monitor in situ degradation of PAHs. Moreover, hydrogen isotopes of benzo(a)pyrene evidenced a degradation process of unknown origin occurring in the darkness. (C) 2016 Elsevier Ltd. All rights reserved

"Piénsatelo bien": reflexiones sobre el papel de la educación para un consumo más responsable

[p.4] Es busquen persones conscients[p.10] Aprenem per a un altre món[p.15] Educar-nos en el quotidià[p.19] La compra pública sostenible[p.31] Educar en el consum[p.39] Contextualitzant l'aprenentatge[p.50] Entrevista amb la Montse Peiron, directora de “Opcions”[p.35] Tejiendo la sostenibilidadPeer Reviewe

A web-based physical activity promotion intervention for inactive parent-child dyads: Protocol for a randomized controlled trial

Background: Low levels of physical activity are associated with numerous adverse health outcomes, yet sedentary lifestyles are common among both children and adults. Physical activity levels tend to decline steeply among children aged between 8 and 12 years, even though children's behavioral patterns are largely governed by familial structures. Similarly, parents' activity levels have been generally reported as lower than those of nonparents of comparable age. For this reason, family-based physical activity promotion interventions are a potentially valuable and relatively underresearched method for mitigating physical activity declines as children develop into adolescents and for increasing physical activity in parents. Objective: This study aims to assess the efficacy, feasibility, and acceptability of a novel theory-based web-based physical activity promotion intervention among parent-child dyads in Finland who do not meet physical activity recommendations at baseline. Methods: Participants (target N=254) will be recruited from the general population using a panel company and advertisements on social media and randomly assigned to either an immediate intervention group or a waitlist control group. The intervention consists of 4 web-based group workshops over the course of 10 weeks, web-based tasks and resources, and a social support chat group. Data on physical activity behavior and constructs from the integrated behavior change model will be collected through self-report surveys assessing physical activity, autonomy support, autonomous motivation, attitude, subjective norm, perceived behavioral control, intention, self-monitoring, habit, and accelerometer measurements at baseline, post intervention, and 3 months post intervention. Exit interviews with participants will assess the feasibility and acceptability of the intervention procedures. Results: This study will reveal whether the intervention changes leisure-time physical activity among intervention participants relative to the control group and will examine the intervention's effects on important theoretical predictors of physical activity. It will also yield data that can be used to refine intervention materials and inform further implementation. Trial recruitment commenced in September 2023, and data collection should be completed by December 2024. Conclusions: The planned intervention has potential implications for both theory and practice. Practically, the use of an entirely web-based intervention may have scalable future uses for improving physical activity in 2 key populations, while also potentially informing on the value of dyadic, family-based strategies for encouraging an active lifestyle as an alternative to strategies that target either parents or children independently. Further, by assessing change in psychological constructs alongside potential change in behavior, the intervention also allows for important tests of theory regarding which constructs are most linked to favorable behavior change outcomes

Climate change impact on the PAH photodegradation in soils: Characterization and metabolites identification

Polycyclic aromatic hydrocarbons (PAHs) are airborne pollutants that are deposited on soils. As climate change is already altering temperature and solar radiation, the global warming is suggested to impact the environmental fate of PAHs. This study was aimed at evaluating the effect of climate change on the PAH photodegradation in soils. Samples ofMediterranean soilswere subjected to different temperature and light radiation conditions in a climate chamber. Two climate scenarios were considered according to IPCC projections: 1) a base (B) scenario, being temperature and light intensity 20 °C and 9.6W/m2, respectively, and 2) a climate change (CC) scenario,working at 24 °C and 24W/m2, respectively. As expected, low molecularweight PAHswere rapidly volatilizedwhen increasing both temperature and light intensity. In contrast, medium and high molecular weight PAHs presented different photodegradation rates in soils with different texture, which was likely related to the amount of photocatalysts contained in both soils. In turn, the hydrogen isotopic composition of some of the PAHs under study was also investigated to verify any degradation process. Hydrogen isotopes confirmed that benzo(a)pyrene is degraded in both B and CC scenarios, not only under light but also in the darkness, revealing unknown degradation processes occurring when light is lacking. Potential generation pathways of PAH photodegradation by-products were also suggested, being a higher number of metabolites formed in the CC scenario. Consequently, in a more or less near future, although humans might be less exposed to PAHs, they could be exposed to new metabolites of these pollutants, which might be even more toxic

Health Risks of Environmental Exposure to PCDD/Fs near a Hazardous Waste Incinerator in Catalonia, Spain

In order to evaluate the environmental impact of a hazardous waste incinerator (HWI) located in Constantí (Spain), during the period 2010-2012, 30 vegetation and soil samples were alternatively (herbage in 2010 and 2012, and soils in 2011) collected in the surroundings of the facility, and their contents of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) determined. Data were compared with those of previous campaigns, and especially with PCDD/F levels in the same monitors reported in the baseline study (1998), when the facility was being constructed. In general terms, a continuous decrease of PCDD/Fs in soils was observed, while in herbage the results indicated an increase since 2008. Anyhow, the health risks associated to environmental exposure of PCDD/Fs are currently within acceptable ranges

The Role of Iron Oxide on the Photodegradation of Polycyclic Aromatic Hydrocarbons: Characterization and Toxicity

<p>Laboratory experiments were conducted to study the photocatalytic capability of amorphous α-Fe₂O₃ to degrade polycyclic aromatic hydrocarbons (PAHs). Solutions containing 16 US EPA priority PAHs were spiked with Fe₂O₃ and incubated in a climate chamber at stable conditions of temperature (20°C) and light (9.6 W·m⁻²) for 28 days. In addition, samples without Fe₂O₃ were also incubated. PAHs levels were monitored by means of GC–MS, and their toxicity was assessed using Microtox® bioassay. Fe₂O₃ had a significant photocatalytic effect on fluorene, phenanthrene, and benzo(<i>a</i>)pyrene, the photodegradation rate of the latter being 71% and 50% in presence and absence of Fe₂O₃, respectively. On the other hand, photolysis was significantly higher than photocatalysis for benzo(<i>a</i>)anthracene and dibenzo(<i>ah</i>)anthracene. Photodegradation of anthracene was notably the same regardless of Fe₂O₃ presence. Remaining PAHs were not photodegraded, neither with, nor without Fe₂O₃. These results indicated that, in some cases, Fe₂O₃ might act as a shield for PAHs by hindering their photodegradation. Other factors, such as the chemical structure of the Fe₂O₃, and also the absence of the soil matrix components (i.e., humic acid, other metals oxides, and texture) could also play a role. Photodegradation trends were supported by changes of EC₅₀ values, suggesting the formation of more bioavailable or toxic PAHs by-products when Fe₂O₃ and light were present.</p

The reporting and diagnosis of uterine fibroids in the UK : an observational study

Background: Uterine fibroids (UFs) are the most common benign tumour in women, and many undergo hysterectomy or uterus-preserving procedures (UPPs) to manage their symptoms. We aimed to validate the recording of UFs in a primary care database, The Health Improvement Network (THIN), and to determine the incidence of UFs in the UK. Methods: In this observational study, women in THIN aged 15-54 years between January 2000 and December 2009 with no previous record of UFs, hysterectomy or UPPs were identified. Individuals were followed up until there was a Read code indicating UFs, they reached 55 years of age or died, or the study ended. Among those without a UF code, women were identified with a code for hysterectomy, UPPs or heavy menstrual bleeding (HMB). Anonymized patient profiles from each category were randomly selected and reviewed. Subsequently, primary care physicians were asked to complete questionnaires to verify the diagnosis for a randomly selected subgroup. Results: In total, 737,638 women were identified who met the initial inclusion criteria. The numbers of women with a code for UFs, hysterectomy, UPPs and HMB were 9380, 11,002, 3220 and 60,915, respectively; the proportions of confirmed cases of UFs were 88.8, 29.7, 57.7 and 15.9 %. The estimated number of women with UFs was 23,140 (64. 0 % without a recorded UF diagnosis). The overall incidence of UFs was 5.8 per 1000 woman-years. Conclusions: UFs were confirmed in a high proportion of women with UF Read codes. However, almost two-thirds of cases were identified among women with a code for hysterectomy, UPPs or HMB. These results show that UFs are under-recorded in UK primary care, and suggest that primary care physicians tend to code the symptoms of UFs more often than the diagnosis