Background data in the context of Pinus Sylvestris, L. Glued laminated timber manufacturing in Spain

The construction sector is achieving its goal of decarbonization. Bioproducts are known to reduce the environmental footprint of the building process, but it is necessary that we determine their exact environmental value. However, assessing the environmental impact relating to buildings is challenging due to a lack of data. The objective of this study was to generate background datasets contextualized to Pinus sylvestrys, L. glulam manufacturing in Spain and apply those datasets to a cradle-to-gate life cycle assessment (LCA) to evaluate both embodied energy (EE) and carbon (EC), as well as biogenic carbon and emissions to air. The corresponding raw materials and energy flows required to apply the LCA methodology were gathered and processed from information from the Spanish forest and wood industry. The resulting background datasets include 27 vehicles and machines, which allowed the quantification of four impact category indicators: renewable primary energy (resources), non-renewable primary energy (resources), use of renewable secondary fuels and global warming potential. Biogenic carbon was also calculated. Based on those five values, the embodied energy and carbon of Pinus sylvestris, L. glulam were quantified: EE = 1401 MJ/UD and EC = −724 kgCO2-eq/UD. The generation of background datasets and environmental information is innovative and of great interest, and it is a powerful tool for prescribers and technicians

Streptococcus lutetiensis Bacteremia. First Clindamycin Resistant Isolate Carrying lnuB Gene

First Case of Streptococcus lutetiensis Bacteremia Involving a Clindamycin-Resistant Isolate Carrying the lnuB Gene.Fil: Almuzara, Marisa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Bonofiglio, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cittadini, Roberto Arnaldo. Instituto Nacional de Tecnologia Agropecuaria; Argentina. Sanatorio Mater Dei; ArgentinaFil: Vera Ocampo, Cecilia. Sanatorio Mater Dei; ArgentinaFil: Montilla, A.. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Houssay. Instituto de Inmunologia, Genetica y Metabolismo; ArgentinaFil: del Castillo, M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; ArgentinaFil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vay, Carlos Alberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentin

Rubric for the evaluation of the TGW

Rúbrica para la evaluación del Trabajo Grupal Tutorizado de la asignatura de Psicología de la Intervención Social y ComunitariaRubric for the evaluation of the Group Work Tutorials of the Psychology of Social and Community Intervention course

Rúbrica per a l'avaluació del Treball Grupal Tutoritzat

Rúbrica per a l'avaluació del Treball Grupal Tutoritzat de l'assignatura Psicologia de la intervenció social i comunitària

ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice

ICAP-1 regulates β1-integrin activation and cell adhesion. Here, we used ICAP-1-null mice to study ICAP-1 potential involvement during immune cell development and function. Integrin α4β1-dependent adhesion was comparable between ICAP-1-null and control thymocytes, but lack of ICAP-1 caused a defective single-positive (SP) CD8+ cell generation, thus, unveiling an ICAP-1 involvement in SP thymocyte development. ICAP-1 bears a nuclear localization signal and we found it displayed a strong nuclear distribution in thymocytes. Interestingly, there was a direct correlation between the lack of ICAP-1 and reduced levels in SP CD8+ thymocytes of Runx3, a transcription factor required for CD8+ thymocyte generation. In the spleen, ICAP-1 was found evenly distributed between cytoplasm and nuclear fractions, and ICAP-1–/– spleen T and B cells displayed upregulation of α4β1-mediated adhesion, indicating that ICAP-1 negatively controls their attachment. Furthermore, CD3+- and CD19+-selected spleen cells from ICAP-1-null mice showed reduced proliferation in response to T- and B-cell stimuli, respectively. Finally, loss of ICAP-1 caused a remarkable decrease in marginal zone B- cell frequencies and a moderate increase in follicular B cells. Together, these data unravel an ICAP-1 involvement in the generation of SP CD8+ thymocytes and in the control of marginal zone B-cell numbers

Diseño y caracterización de nuevas soluciones constructivas en base madera

Máster en Ingeniería de Montes.[ES]: El creciente interés por la madera se está haciendo cada vez más evidente en los últimos años. Arquitectos e ingenieros están cada vez más receptivos en cuanto a su uso debido a los valores asociados a la sostenibilidad. La madera además de ser un magnífico material de construcción es renovable y procede de montes certificados. Consumir madera en la construcción supone una forma clara de lucha contra el cambio climático. El presente Trabajo Profesional se desarrolla ligado al Proyecto Grupo Operativo Construcción Madera Sostenible “Herramienta inteligente para la selección de productos de madera destinados a la construcción”, que tiene como fin facilitar la prescripción de productos de madera en el sector de la construcción. En este trabajo se pretende (1) proporcionar información sobre productos de madera de la construcción y establecer una vinculación con soluciones constructivas, (2) aportar nuevas soluciones constructivas en base a madera, (3) caracterizarlas mediante datos técnicos fiables y definir sus prestaciones. Se establece una clasificación jerárquica de soluciones constructivas de la que se obtiene 40 soluciones de cubierta, 12 de fachada, 8 forjados y 24 particiones verticales. De estas se elaboran 24 fichas de soluciones constructivas con detalles técnicos de elementos constructivos, materiales y prestaciones. Además, se lleva a cabo una revisión de productos de madera en la construcción que permite obtener un análisis completo de las soluciones constructivas y los elementos de madera que las componen. Esto podrá ayudar a la elección de productos de madera por parte del sector de la construcción.[EN]: The growing interest in wood is becoming increasingly evident in recent years. Architects and engineers are receptive to their use due to the values associated with sustainability. In addition to being a magnificent building material, wood is renewable and comes from certified forests. Consuming wood in construction is a clear way to fight climate change. This Professional Work is carried out linked to the Sustainable Wood Construction Operational Group Project "Smart tool for the selection of wood products for construction", which aims to facilitate the prescription of wood products in the construction sector. This work aims to (1) provide information on construction wood products and establish a link with constructive solutions, (2) provide new constructive solutions based on wood, (3) characterize them through reliable technical data and define their performance. A hierarchical classification of constructive solutions is established from which 40 roof solutions, 12 facade, 8 slabs and 24 vertical partitions are obtained. Of these, 24 sheets of constructive solutions are elaborated with technical details of constructive elements, materials and features. In addition, a review of wood products in the construction is carried out that allows to obtain a complete analysis of the constructive solutions and the wooden elements that compose them. This may help the choice of wood products by the construction sector.Peer reviewe

2244 European Environmental Databases. OpenDAP, Spanish context.

With the aim of facilitating sustainability assessment through Life Cycle Assessment, the Eduardo Torroja Institute of Construction Sciences. CSIC is developing a public database of environmental information: OpenDAP. One of the objectives of this database is to be compatible with other European databases but to be fully open, public and free of charge. With the participation in the project “Wood for Sustainable Construction Task Force”, whose coordinator was the University of Cordoba, 10 EPDs have been developed for different wood products and species, with a detailed study of each stage, using a spreadsheet and field studies for data collection.In this article we aim to demonstrate the need for environmental quantification of services and products in the construction sector, due to the importance of the global impact of this sector. With common procedures that allow us to obtain comparable and useful results for the final client who selects them. Both for generic and specific products, being stable and exportable. Allowing us to compare, reduce and improve our sustainability

In vivo adhesion of malignant B cells to bone marrow microvasculature is regulated by α4β1 cytoplasmic-binding proteins

40 p.-7 fig. Martínez-Moreno, Mónica et al.Multiple myeloma (MM) and chronic lymphocytic leukemia (CLL) cells must attach to the bone marrow (BM) microvasculature before lodging in the BM microenvironment. Using intravital microscopy (IVM) of the BM calvariae we demonstrate that the α4β1 integrin is required for MM and CLL cell firm arrest onto the BM microvasculature, while endothelial P-selectin and E-selectin mediate cell rolling. Talin, kindlin-3 and ICAP-1 are β1-integrin-binding partners that regulate β1-mediated cell adhesion. We show that talin and kindlin-3 cooperatively stimulate high affinity and strength of α4β1-dependent MM and CLL cell attachment, whereas ICAP-1 negatively regulates this adhesion. A functional connection between talin/kindlin-3 and Rac1 was found to be required for MM cell attachment mediated by α4β1. Importantly, IVM analyses with talin- and kindlin-3-silenced MM cells indicate that these proteins are needed for cell arrest on the BM microvasculature. Instead, MM cell arrest is repressed by ICAP-1. Moreover, MM cells silenced for talin and kindlin-3, and cultured on α4β1 ligands showed higher susceptibility to bortezomib-mediated cell apoptosis. Our results highlight the requirement of α4β1 and selectins for the in vivo attachment of MM and CLL cells to the BM microvasculature, and indicate that talin, kindlin-3 and ICAP-1 differentially control physiological adhesion by regulating α4β1 activity.This work was supported by the following grants from the Ministerio de Economía y Competitividad (Spain): SAF2011-24022 to J.T; SAF2012-31613 to A.G-P; SAF2012-31142 and SAF2013-49662-EXP to A.H; PI13/01454 to P.S-M; RD12/0036/0061 to J.T. and A.G-P; RD12/0036/0058 to N.C.G. The work was also funded by grant P2010/BMD-2314 from the Comunidad de Madrid to A.G-P., J.T., P. S-M and A.H., and partially supported by a CRIS foundation grant to fight cancer to J.M-L. N.C.G. is also funded by a grant from the Asociación Española Contra el Cáncer (GCB120981SAN), and from the Gerencia Regional de Salud, Junta de Castilla y León (GRS-702/A/11).Peer reviewe

The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination

A mutation in the HERC2 gene has been linked to a severe neurodevelopmental disorder with similarities to the Angelman syndrome. This gene codifies a protein with ubiquitin ligase activity that regulates the activity of tumor protein p53 and is involved in important cellular processes such as DNA repair, cell cycle, cancer, and iron metabolism. Despite the critical role of HERC2 in these physiological and pathological processes, little is known about its relevance in vivo. Here, we described a mouse with targeted inactivation of the Herc2 gene. Homozygous mice were not viable. Distinct from other ubiquitin ligases that interact with p53, such as MDM2 or MDM4, p53 depletion did not rescue the lethality of homozygous mice. The HERC2 protein levels were reduced by approximately one-half in heterozygous mice. Consequently, HERC2 activities, including ubiquitin ligase and stimulation of p53 activity, were lower in heterozygous mice. A decrease in HERC2 activities was also observed in human skin fibroblasts from individuals with an Angelman-like syndrome that express an unstable mutant protein of HERC2. Behavioural analysis of heterozygous mice identified an impaired motor synchronization with normal neuromuscular function. This effect was not observed in p53 knockout mice, indicating that a mechanism independent of p53 activity is involved. Morphological analysis showed the presence of HERC2 in Purkinje cells and a specific loss of these neurons in the cerebella of heterozygous mice. In these animals, an increase of autophagosomes and lysosomes was observed. Our findings establish a crucial role of HERC2 in embryonic development and motor coordination

The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination

A mutation in the HERC2 gene has been linked to a severe neurodevelopmental disorder with similarities to the Angelman syndrome. This gene codifies a protein with ubiquitin ligase activity that regulates the activity of tumor protein p53 and is involved in important cellular processes such as DNA repair, cell cycle, cancer, and iron metabolism. Despite the critical role of HERC2 in these physiological and pathological processes, little is known about its relevance in vivo. Here, we described a mouse with targeted inactivation of the Herc2 gene. Homozygous mice were not viable. Distinct from other ubiquitin ligases that interact with p53, such as MDM2 or MDM4, p53 depletion did not rescue the lethality of homozygous mice. The HERC2 protein levels were reduced by approximately one-half in heterozygous mice. Consequently, HERC2 activities, including ubiquitin ligase and stimulation of p53 activity, were lower in heterozygous mice. A decrease in HERC2 activities was also observed in human skin fibroblasts from individuals with an Angelman-like syndrome that express an unstable mutant protein of HERC2. Behavioural analysis of heterozygous mice identified an impaired motor synchronization with normal neuromuscular function. This effect was not observed in p53 knockout mice, indicating that a mechanism independent of p53 activity is involved. Morphological analysis showed the presence of HERC2 in Purkinje cells and a specific loss of these neurons in the cerebella of heterozygous mice. In these animals, an increase of autophagosomes and lysosomes was observed. Our findings establish a crucial role of HERC2 in embryonic development and motor coordination