Genomic analyses of microdissected Hodgkin and Reed-Sternberg cells: mutations in epigenetic regulators and p53 are frequent in refractory classic Hodgkin lymphoma

This work was supported by grants from the Plan Nacional de I + D + I cofinanced by the ISCIII-Subdirección General de Evaluación and the Fondo Europeo de Desarrollo Regional (FEDER), PI12/1832, the Spanish Association for Cancer Research (AECC), and Programas para Grupos de Investigación de la Comunidad Autónoma de Madrid (Biomedicina 2017)

Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer

Starch films are gaining attention as substitutes of synthetic polymers due to their biodegradability and low cost. Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability. In this work, [emim+][Ac−]-plasticized starch films were prepared from potato, corn and wheat starch. The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated. Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult. In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content. This more crystalline structure could be the responsible of their better mechanical properties. [emim+][Ac−] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.This work was partially supported from the European Commission, European-Union and Ministerio de Economía y Competitividad (MINECO), Spain (Ref. CTQ2016-78246-R) and Generalitat Valenciana (Project PROMETEOII/2014/007). M.G. Montalbán acknowledges support from Ministerio de Economía y Competitividad (MINECO) (Juan de la Cierva-Formación contract, Ref. FJCI-2016-28081)

Nuevos registros y ampliación de la distribución de anfibios y reptiles en el Estado de Morelos, México.

During new herpetological explorations we found three herpetological species previously unknown in the state of Morelos, and three other species extending their range in the Balsas River Basin. The herpetofauna of Morelos is currently represented by 123 species

Spanish Lymphoma Group (GELTAMO) guidelines for the diagnosis, staging, treatment, and follow-up of diffuse large B-cell lymphoma

Diffuse large B-cell lymphoma (DLBCL) accounts for approximately 30% of non-Hodgkin lymphoma (NHL) cases in adult series. DLBCL is characterized by marked clinical and biological heterogeneity, encompassing up to 16 distinct clinicopathological entities. While current treatments are effective in 60% to 70% of patients, those who are resistant to treatment continue to die from this disease. An expert panel performed a systematic review of all data on the diagnosis, prognosis, and treatment of DLBCL published in PubMed, EMBASE and MEDLINE up to December 2017. Recommendations were classified in accordance with the Grading of Recommendations Assessment Development and Evaluation (GRADE) framework, and the proposed recommendations incorporated into practical algorithms. Initial discussions between experts began in March 2016, and a final consensus was reached in November 2017. The final document was reviewed by all authors in February 2018 and by the Scientific Committee of the Spanish Lymphoma Group GELTAMO

Contrato de trabajo, compromiso y satisfacción: Moderación de la empleabilidad

La búsqueda de prácticas flexibles de gestión de los recursos humanos viene desempeñando un papel importante para el desarrollo estratégico de las organizaciones y en particular en contextos de profunda crisis económica y financiera como la actual en Portugal, con una situación de desempleo creciente y un clima generalizado de inseguridad laboral. Este estudio pretende examinar si la percepción de empleabilidad por parte de los trabajadores puede desempeñar un papel moderador en la relación entre formas más flexibles de contratación, como el trabajo temporal, y las actitudes laborales. Resultados sugieren que la percepción de empleabilidad puede tener un efecto moderador en la relación entre el tipo de contrato de trabajo y el compromiso afectivo y la satisfacción laboral de los trabajadores bajo contrato directo con la organización pero esta interacción no se ha encontrado para trabajadores con contrato de trabajo temporal con las Agencias de Empleo. ------ ABSTRACT ------ The search for flexible practices of human resources management comes playing an important role for the strategic organizational development and specially in contexts of deep economic and financial crisis like the present one in Portugal, with an increasing unemployment and a generalized climate of job insecurity. This study tries to examine if perception of employability by employees moderates the relationship between flexible forms of hiring, such as temporary work, and job attitudes like affective commitment and job satisfaction. Results suggest the moderating role of employability for workers under direct contract with the organization but this interaction has not been pointed for workers with an temporary work contract with Employment Agencies. ------ RESUMO ------- A busca por práticas flexíveis da gestão dos recursos humanos vem desempenhando um papel importante para o desenvolvimento estratégico das organizações e, em particular, nos contextos da profunda crise econômica e financeira como a que vive hoje Portugal, com uma situação de desemprego crescente e um clima generalizado de insegurança laboral. Este estudo pretende examinar se a percepção de empregabilidade por parte dos trabalhadores pode desempenhar um papel moderador na relação entre as formas mais flexíveis de contratação, como o trabalho temporário, e as atitudes laborais. Resultados sugerem que a percepção de empregabilidade pode ter um efeito moderador na relação entre o tipo de contrato de trabalho e o compromisso efetivo e a satisfação laboral dos trabalhadores com contrato direto com a organização, mas esta interação não foi encontrada para os trabalhadores com contrato de trabalho temporário com as Agências de Emprego

Influence of Starch Composition and Molecular Weight on Physicochemical Properties of Biodegradable Films

Thermoplastic starch (TPS) films are considered one of the most promising alternatives for replacing synthetic polymers in the packaging field due to the starch biodegradability, low cost, and abundant availability. However, starch granule composition, expressed in terms of amylose content and phosphate monoesters, and molecular weight of starch clearly affects some film properties. In this contribution, biodegradable TPS films made from potato, corn, wheat, and rice starch were prepared using the casting technique. The effect of the grain structure of each starch on microstructure, transparency, hydration properties, crystallinity, and mechanical properties of the films, was evaluated. Potato starch films were the most transparent and corn starch films the most opaque. All the films had homogeneous internal structures—highly amorphous and with no pores, both of which point to a good starch gelatinization process. The maximum tensile strength (4.48–8.14 MPa), elongation at break (35.41–100.34%), and Young’s modulus (116.42–294.98 MPa) of the TPS films were clearly influenced by the amylose content, molecular weight, and crystallinity of the film. In this respect, wheat and corn starch films, are the most resistant and least stretchable, while rice starch films are the most extensible but least resistant. These findings show that all the studied starches can be considered suitable for manufacturing resistant and flexible films with similar properties to those of synthetic low-density polyethylene (LDPE), by a simple and environmentally-friendly process.This work was partially supported by the European Commission (FEDER/ERDF), the Spanish MINECO (Ref. CTQ2016-78246-R), and Generalitat Valenciana (Project PROMETEOII/2014/007). M.G.M. acknowledges support from MINECO (Juan de la Cierva-Formación contract, Ref. FJCI-2016-28081)

Study of the behavior of biodegradable starch/polyvinyl alcohol/rosin blends

Biodegradable potato starch/PVA samples containing different concentrations of rosin were prepared by melt-mixing in order to study the enhancement of the properties of native starch films. Glycerol and polyvinyl alcohol (PVA) are commonly used as plasticizers of starch. Their relatively low molecular weight (compared with starch) contributes to a good processability. Rosin is a renewable product whose incorporation in the starch/PVA matrix induces processing aid and reinforcing effects. Its relatively high molecular weight might prevent its migration to the surface of the final product. Water content, solubility in water, mechanical properties, microstructure and dynamic mechanical analysis of the samples were studied. The addition of 8% rosin to starch/PVA blends led to tensile strength values higher than 10 MPa and elongation at break values close to 2000%, values comparable to those offered by conventional polymers used in food packaging, for example LDPE. Furthermore, starch compounds have low cost and high biodegradability.This work was partially supported from the European Commission (FEDER/ERDF) and the Spanish MINECO (Ref. CTQ2016-78246-R) and Generalitat Valenciana (Project PROMETEOII/2014/007). M.G. Montalbán acknowledges support from MINECO (Juan de la Cierva-Formación contract, Ref. FJCI-2016-28081)

Análisis de la amplitud acomodativa en sujetos miopes tras el implante de lente intraocular fáquica de cámara posterior

La cirugía refractiva con LIO fáquica es una alternativa eficaz para la corrección de defectos refractivos y conseguir independencia de las gafas y lentes de contacto, sobre todo en altas ametropías. Sin embargo, la escasez de evidencia científica que describa cómo responde el sistema acomodativo a la implantación de lentes ICL hace imprescindible seguir investigando. La Optometría juega un papel fundamental en este punto, ya que debe adaptarse a la aparición de nuevas técnicas de compensación visual y profundizar en su conocimiento. Sólo de esta forma se podrán mejorar los protocolos pre y postoperatorios, minimizar las complicaciones relacionadas con el sistema acomodativo y tratar las disfunciones visuales que puedan estar causadas por la cirugía

Analysis of the mutational landscape of classic Hodgkin lymphoma identifies disease heterogeneity and potential therapeutic targets

Defining the mutational landscape of classic Hodgkin lymphoma is still a major research goal. New targeted next-generation sequencing (NGS) techniques may identify pathogenic mechanisms and new therapeutic opportunities related to this disease. We describe the mutational profile of a series of 57 cHL cases, enriched in Hodgkin and Reed-Sternberg (HRS) cells. Overall, the results confirm the presence of strong genomic heterogeneity. However, several variants were consistently detected in genes related to relevant signaling pathways, such as GM-CSF/IL-3, CBP/EP300, JAK/STAT, NF-kappaB, and numerous variants of genes affecting the B-cell receptor (BCR) pathway, such as BTK, CARD11, BCL10, among others. This unexpectedly high prevalence of mutations affecting the BCR pathway suggests some requirement for active BCR signaling for cHL cell viability. Additionally, incubation of a panel of cHL cellular models with selective BTK inhibitors in vitro constrains cell proliferation and causes cell death. Our results indicate new pathogenic mechanisms and therapeutic opportunities in this disease