Modification of as-cast Al-Mg/B4C composite by addition of Zr

Zirconium was used in Al-Mg/B4C composite to improve compocasting efficiency by increasing particle incorporation. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) results revealed that by addition of zirconium a reaction layer containing Zr, Al, B and C is formed on the interface of B4C-matrix. X-ray diffraction (XRD) analysis of extracted particles unveiled that the ZrB2 phase is the main constituent of this layer. Formation of ZrB2 is an exothermic reaction which can rise temperature locally around particles and agglomerates. Rising temperature around agglomerates in conjunction with turbulent flow of melt facilitates agglomerates wetting and dissolving into molten aluminum. As the result, final product contains more uniformly distributed B4C particles. Besides enhancing compocasting efficiency, addition of Zr and formation of reaction layer by improving particle matrix bonding quality, led to increase in ultimate tensile strength and elongation of the composite around 8% and 30%, respectively. SEM observations of the fracture surfaces confirmed that a proper bonding presents at the interface of particles and matrix in presence of Zr.Peer ReviewedPostprint (author's final draft

Silymarin-albumin nanoplex: preparation and its potential application as an antioxidant in nervous system in vitro and in vivo

In this study, we formulated silymarin-HSA nanoplex and assayed its ability to reduce LPSinduced toxicity in vitro and in vivo. Silymarin molecules were encapsulated into HSA nanoplex and the loading efficiency and characterization of fabricated nanoplex were performed by using HPLC, TEM, SEM, DLS, FTIR analysis, and theoretical studies. Afterwards, their protective effect against LPS (20 µg/ml) -induced toxicity in SH-SY5Y cells was investigated by MTT, ROS, and apoptosis assays. For in vivo experiments, rats were pre-treated with either silymarin or silymarin -HSA nanoplex (200 mg/kg) orally for 3 days and at third day received LPS by IP at a dose of 0.5 mg/kg, 150 min before scarification followed by SOD and CAT activity assay. The formulation of silymarin-HSA nanoplex showed a spherical shape with an average diameter between 50 nm to 150 nm, hydrodynamic radius of 188.3 nm, zeta potential of -26.6 mV, and a drug loading of 97.3%. In LPS-treated cells, pretreatments with silymarin-HSA noncomplex recovered the cell viability and decreased the ROS level and corresponding apoptosis more significantly than free silymarin. In rats, it was also depicted that, silymarin-HSA noncomplex can increase the SOD and CAT activity in brain tissue at LPS-triggered oxidative stress model more significantly than free counterpart. Nanoformulation of silymarin improved its capability to reduce LPS-induced oxidative stress by restoring cell viability and elevation of SOD and CAT activity in vitro and in vivo, respectively. Therefore, formulation of silymarin may hold a great promise in the field of antioxidant agent development

Synthesis and Characterization of a New ZIF-67@MgAl2O4 Nanocomposite and Its Adsorption Behaviour

Fabricating suitable adsorbents with low-cost and high efficiency extraction for measurement of very small amounts of agricultural pesticides in food and water is playing a vital key role in personal and environmental health. Here, a new composite of zeolitic imidazolate framework-67@magnesium aluminate spinel (ZIF-67@MgAl2O4) has been fabricated by a simple method at room temperature with different weight ratios. Several techniques such as FE-SEM, BET, XRD, and TGA have been used to confirm the structural characterization of the obtained materials. The obtained ZIF-67@MgAl2O4 was utilized as an adsorbent in the solid phase microextraction technique to extract and preconcentrate the herbicide molinate (as an analyte) in aqueous solution. Corona discharge ionization-ion mobility spectrometry (CD-IMS) was applied for quantification of the analyte molecules. Extraction temperature, extraction time, stirring rate, and sample pH as the main parameters that affected the extraction proficiency were chosen and considered. Under optimal conditions, the linear dynamic range (LDR) of the various concentrations of the molinate and correlation coefficient were 10.0-100.0 mu g L-1 and 0.9961, respectively. The limit of quantification (LOQ) and method detection limit (MDL) were 10.0 mu g L-1 and 3.0 mu g L-1, respectively. The relative standard deviation (RSD) of the ZIF-67@MgAl2O4 for extracting the molinate molecules (molinate concentration; 50 mu g L-1) was calculated to be 4% and the enrichment factor (EF) was similar to 5.The authors of the article are delighted to manifest their gratitude Isfahan University of Technology for nancial support. Furthermore, AES is grateful for the National Research grants from MINECO, Spain, “Juan de la Cierva” [FJCI-2018-037717

Modification of as-cast Al-Mg/B4C composite by addition of Zr

Zirconium was used in Al-Mg/B4C composite to improve compocasting efficiency by increasing particle incorporation. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) results revealed that by addition of zirconium a reaction layer containing Zr, Al, B and C is formed on the interface of B4C-matrix. X-ray diffraction (XRD) analysis of extracted particles unveiled that the ZrB2 phase is the main constituent of this layer. Formation of ZrB2 is an exothermic reaction which can rise temperature locally around particles and agglomerates. Rising temperature around agglomerates in conjunction with turbulent flow of melt facilitates agglomerates wetting and dissolving into molten aluminum. As the result, final product contains more uniformly distributed B4C particles. Besides enhancing compocasting efficiency, addition of Zr and formation of reaction layer by improving particle matrix bonding quality, led to increase in ultimate tensile strength and elongation of the composite around 8% and 30%, respectively. SEM observations of the fracture surfaces confirmed that a proper bonding presents at the interface of particles and matrix in presence of Zr.Peer Reviewe

Cell-imprinted substrates act as an artificial niche for skin regeneration

Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications

Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration

Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly­(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications

Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration

Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly­(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications

Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration

Bioinspired materials can mimic the stem cell environment and modulate stem cell differentiation and proliferation. In this study, biomimetic micro/nanoenvironments were fabricated by cell-imprinted substrates based on mature human keratinocyte morphological templates. The data obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly­(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications