Role of shear stress in endothelial cell morphology and expression of cyclooxygenase isoforms

MEDLINE® is the source for the MeSH terms of this document.Objective-: The goal of this study was to examine the effect of chronic heterogeneous shear stress, applied using an orbital shaker, on endothelial cell morphology and the expression of cyclooxygenases 1 and 2. Methods and results-: Porcine aortic endothelial cells were plated on fibronectin-coated Transwell plates. Cells were cultured for up to 7 days either under static conditions or on an orbital shaker that generated a wave of medium inducing shear stress over the cells. Cells were fixed and stained for the endothelial surface marker CD31 or cyclooxygenases 1 and 2. En face confocal microscopy and scanning ion conductance microscopy were used to show that endothelial cells were randomly oriented at the center of the well, aligned with shear stress nearer the periphery, and expressed cyclooxygenase-1 under all conditions. Lipopolysaccharide induced cyclooxygenase-2 and the production of 6-keto-prostaglandin F1α in all cells. Conclusion-: Cyclooxygenase-1 is expressed in endothelial cells cultured under chronic shear stress of high or low directionality.Peer reviewedSubmitted Versio

Host List of Plants Susceptible to Tomato Spotted Wilt Virus (TSWV)

Tomato spotted wilt virus (TSWV)-infected plant hosts in vegetable-growing regions play an important role in the epidemiology of the disease. Susceptible hosts are commonly found growing along field borders and among crops of Hawaii's major vegetable-growing regions. Elimination of these TSWV reservoirs should be helpful in reducing disease losses. This paper presents an up-to-date listing of TSWV-susceptible plant species

Bio-Inspired Load Balancing In Large-Scale WSNs Using Pheromone Signalling

Wireless sensor networks (WSNs) consist of multiple, distributed nodes each with limited resources. With their strict resource constraints and application-specific characteristics, WSNs contain many challenging tradeoffs. This paper proposes a bioinspired load balancing approach, based on pheromone signalling mechanisms, to solve the tradeoff between service availability and energy consumption. We explore the performance consequences of the pheromone-based load balancing approach using (1) a system-level simulator, (2) deployment of real sensor testbeds to provide a competitive analysis of these evaluation methodologies. The effectiveness of the proposed algorithm is evaluated with different scenario parameters and the required performance evaluation techniques are investigated on case studies based on sound sensors

Social determinants of smoking in women from low-income rural backgrounds: Findings from a photovoice study

Smoking is well recognized as one of the most preventable causes of death and disease in the United States (U.S.), accounting for approximately 480,000 deaths each year (Centers for Disease Control and Prevention [CDC], 2014). In addition, the American Academy of Addiction Psychiatry (AAAP, 2018) estimates that approximately 20–30% of individuals experimenting with cigarettes will meet criteria for tobacco use disorder within their lifetime while 60–80% of current cigarette smokers manifest classic drug dependence criteria. Associated annual healthcare cost of smoking are approximately133 billion dollars, with the additional cost in lost productivity estimated at 156 billion dollars annually (CDC, 2014; United States Department of Health and Human Services [USDHHS], 2014). Although recent national rates of cigarette smoking in adults have declined from 20.9% in 2005 to 13.7% in 2018, there remains significant disparity in smoking rates among rural populations (Creamer et al., 2019)

Thermodynamic and Kinetic Parameters for Calcite Nucleation on Peptoid and Model Scaffolds:A Step toward Nacre Mimicry

The production of novel composite materials, assembled using biomimetic polymers known as peptoids (N-substituted glycines) to nucleate CaCO3, can open new pathways for advanced material design. However, a better understanding of the heterogeneous CaCO3 nucleation process is a necessary first step. We determined the thermodynamic and kinetic parameters for calcite nucleation on self-assembled monolayers (SAMs) of nanosheet-forming peptoid polymers and simpler, alkanethiol analogues. We used nucleation rate studies to determine the net interfacial free energy (γ net) for the peptoid-calcite interface and for SAMs terminated with carboxyl headgroups, amine headgroups, or a mix of the two. We compared the results with γ net determined from dynamic force spectroscopy (DFS) and from density functional theory (DFT), using COSMO-RS simulations. Calcite nucleation has a lower thermodynamic barrier on the peptoid surface than on carboxyl and amine SAMs. From the relationship between nucleation rate (J 0) and saturation state, we found that under low-saturation conditions, i.e. <3.3 (pH 9.0), nucleation on the peptoid substrate was faster than that on all of the model surfaces, indicating a thermodynamic drive toward heterogeneous nucleation. When they are taken together, our results indicate that nanosheet-forming peptoid monolayers can serve as an organic template for CaCO3 polymorph growth

Efeitos da densidade de população de plantas na cultura de couve-flor (Brassica oleracea L. var. botrytis)

An experiment was carried out to study the effects of the following population densities cauliflowers (plants per ha): 20,833 (0.60 m x 0.80 m), 25,641 (0.60 m x 0.65 m), ....37.037 (0.60 m x 0.45 m) , 55.555 (.0.60 m x 0.30 m), and 111,111 (0,60 m x 0,15 m) ; variety Snow ball. It was concluded that the effects of plant population density are greater on curd quality (weight and size) than on production per ha. The best plant population density to produce cauliflowers curd for Brazil market is from 20,000 to 25,000 plants/ha while for mini-curd is above 55,000 plants/ha.O experimento foi instalado na área experimental do Setor de Horticultura da ESALQ. (Piracicaba, SP), em um Latossol Roxo série "Luiz de Queiroz", em março de 1977, considerando as seguintes densidades de população: 20.833 plantas/ha (0,60 m x 0,80m), .. 25.641 plantas/ha (0,60 m x 0,65 m), 37.037 plantas/ha (..0.,60 m x 0,45 ml, 55.550 plantas/ha (,06Q m x 0,30 ,) e 111.111 plantas/ ha (0,60 m x 0,15 m). A partir dos resultados obtidos e para as condições do experimento concluiu-se que a densidade de população sobre a produção de couve-flor afeta mais a qualidade da cabeça (peso e tamanho), enquanto que o rendimento por área é pouco afetado. Para as condições do nosso mercado, a densidade ótima deve estar entre 20.000 a 25.000 plantas por ha e para a produção de mini-couve-flor mais de 55.000 plantas por ha, paraocultivar Bola de Neve

Precision Measurement of the Proton and Deuteron Spin Structure Functions g2 and Asymmetries A2

We have measured the spin structure functions g2p and g2d and the virtual photon asymmetries A2p and A2d over the kinematic range 0.02 < x < 0.8 and 0.7 < Q^2 < 20 GeV^2 by scattering 29.1 and 32.3 GeV longitudinally polarized electrons from transversely polarized NH3 and 6LiD targets. Our measured g2 approximately follows the twist-2 Wandzura-Wilczek calculation. The twist-3 reduced matrix elements d2p and d2n are less than two standard deviations from zero. The data are inconsistent with the Burkhardt-Cottingham sum rule if there is no pathological behavior as x->0. The Efremov-Leader-Teryaev integral is consistent with zero within our measured kinematic range. The absolute value of A2 is significantly smaller than the sqrt[R(1+A1)/2] limit.Comment: 12 pages, 4 figures, 2 table

Measurement of the Proton and Deuteron Spin Structure Functions g2 and Asymmetry A2

We have measured the spin structure functions g2p and g2d and the virtual photon asymmetries A2p and A2d over the kinematic range 0.02 < x < 0.8 and 1.0 < Q^2 < 30(GeV/c)^2 by scattering 38.8 GeV longitudinally polarized electrons from transversely polarized NH3 and 6LiD targets.The absolute value of A2 is significantly smaller than the sqrt{R} positivity limit over the measured range, while g2 is consistent with the twist-2 Wandzura-Wilczek calculation. We obtain results for the twist-3 reduced matrix elements d2p, d2d and d2n. The Burkhardt-Cottingham sum rule integral - int(g2(x)dx) is reported for the range 0.02 < x < 0.8.Comment: 12 pages, 4 figures, 1 tabl

Measurements of the Q2Q^2-Dependence of the Proton and Neutron Spin Structure Functions g1p and g1n

The structure functions g1p and g1n have been measured over the range 0.014 < x < 0.9 and 1 < Q2 < 40 GeV2 using deep-inelastic scattering of 48 GeV longitudinally polarized electrons from polarized protons and deuterons. We find that the Q2 dependence of g1p (g1n) at fixed x is very similar to that of the spin-averaged structure function F1p (F1n). From a NLO QCD fit to all available data we find $\Gamma_1^p - \Gamma_1^n =0.176 \pm 0.003 \pm 0.007$ at Q2=5 GeV2, in agreement with the Bjorken sum rule prediction of 0.182 \pm 0.005.Comment: 17 pages, 3 figures. Submitted to Physics Letters