Motile sperm organelle morphology examination (MSOME): intervariation study of normal sperm and sperm with large nuclear vacuoles

<p>Abstract</p> <p>Background</p> <p>Although the motile sperm organelle morphology examination (MSOME) was developed only as a selection criterion, its application as a method for classifying sperm morphology may represent an improvement in evaluation of semen quality, with potential clinical repercussions. The present study aimed to evaluate individual variations in the motile sperm organelle morphology examination (MSOME) analysis after a time interval.</p> <p>Methods</p> <p>Two semen samples were obtained from 240 men from an unselected group of couples undergoing infertility investigation and treatment. Mean time interval between the two semen evaluations was 119 +/- 102 days. No clinical or surgical treatment was realized between the two observations. Spermatozoa were analyzed at greater than or equal to 8400× magnification by inverted microscope equipped with DIC/Nomarski differential interference contrast optics. At least 200 motile spermatozoa per semen sample were evaluated and percentages of normal spermatozoa and spermatozoa with large nuclear vacuoles (LNV/one or more vacuoles occupying >50% of the sperm nuclear area) were determined. A spermatozoon was classified as morphologically normal when it exhibited a normal nucleus (smooth, symmetric and oval nucleus, width 3.28 +/- 0.20 μm, length 4.75 +/- 0.20 μm/absence of vacuoles occupying >4% of nuclear area) as well as acrosome, post-acrosomal lamina, neck and tail, besides not presenting cytoplasm around the head. One examiner, blinded to subject identity, performed the entire study.</p> <p>Results</p> <p>Mean percentages of morphologically normal and LNV spermatozoa were identical in the two MSOME analyses (1.6 +/- 2.2% vs. 1.6 +/- 2.1% <it>P </it>= 0.83 and 25.2 +/- 19.2% vs. 26.1 +/- 19.0% <it>P </it>= 0.31, respectively). Regression analysis between the two samples revealed significant positive correlation for morphologically normal and for LNV spermatozoa (r = 0.57 95% CI:0.47-0.65 <it>P </it>< 0.0001 and r = 0.50 95% CI:0.38-0.58 <it>P </it>< 0.0001, respectively).</p> <p>Conclusions</p> <p>The significant positive correlation and absence of differences between two sperm samples evaluated after a time interval with respect to normal morphology and LNV spermatozoa indicated that MSOME seems reliable (at least for these two specific sperm forms) for analyzing semen. The present result supports the future use of MSOME as a routine method for semen analysis.</p

Россия и Монголия близки

В статье дается обзор презентации монографий российских, монгольских ученых о Монголии и ее культуре, которая состоялась в г. Улабаатор (Монголия)

Actual problems of Eurasianism

Reader’s note on: Eurasianism: theoretical potential and practical application: materials of Sixth All-Russian Science and Practice conference (with international participants) Barnaul, June 25–26, 2012: in 2 v. / ed. by V.Ya. Barkalov, A.V. Ivanov, Barnaul: IG "Si-press”, 2012

Hallmark of Dentistry: The Evolution and Benefits of the Dental Magnifying Loupe

ABSTRACTBackground Dental education has increasingly recognized magnification as an integral part of dentistry and based on the advantages and benefits recommends its use as early as in the first year of dental school. Dental students and practitioners have become aware of their enhanced quality of work, while preventing eye fatigue and strain. Thanks to proper ergonomics, long-term bodily afflictions such as MSDs can be prevented and increase the dental students and practitioners’ ability to enhance career longevity.Case Description This review aims to present the evolution and benefits of dental magnifying loupes used to improve diagnosis and treatment outcomes through enhancement of the visual acuity, thus preventing musculoskeletal disorders and improving ergonomics. An overview of the type of magnification is presented discussing single lens loupes, Galilean loupes and prism loupes. Furthermore, visual acuity, working distance and depth of field, declination angle and working angle, field of view and convergence angle, magnification and illumination are explained.Practical Implications Loupes offer the dental profession enhanced visualization of the operating field, while at the same time they reduce eyestrain providing ergonomic advantages, and preventing long-term complications such as musculoskeletal disorders (MSDs). When dental students and practitioners wear dental loupes in an ergonomically correct neutral position, significantly more benefits have been reported compared to operators that do not use magnification. Dental magnifying loupes are becoming the expected hallmark of dentistry; thus, they are considered a new identity and icon of the dental profession