Submarine lava flow emplacement and faulting in the axial valley of two morphologically distinct spreading segments of the Mariana back-arc basin from Wadatsumi side-scan sonar images

International audienceHigh-resolution, deep-tow side-scan sonar data were collected over two distinct spreading segments in the central part of the Mariana back-arc basin. These data allow mapping of small fissures and faults and the distinguishing of hummocky from smooth lava flows. Using these data, we observe spatial variations in seafloor deformation and volcanic activity within each segment, and also significant differences in the degree of tectonic deformation between the two segments. One segment, characterized by an hourglass shape suggestive of magmatic processes dominating over tectonic processes, is in fact currently dominated by intense deformation rather than volcanism. The other segment, which exhibits morphology (wide and deep linear axial valley) typical of magma-starved segments, is subjected to very limited deformation and is covered by mostly unfaulted, recent flows. Each segment also displays along-axis variations in the degree of tectonic deformation and in lava flows freshness. We observe a decrease of lava effusion rate from segment centers toward their ends. We also investigated the apparent asymmetry of the Mariana basin. On the southern segment of the study area, azimuths of tectonic structures are divided into two groups, one segment-parallel and one ∼15° oblique to strike of the segment. These two trends of linear features developed synchronously with volcanic activity. Currently oblique structures seem to be the most active ones. The tectonic structures that are parallel to the overall valley trend are distributed over the entire valley, whereas the oblique structures are only located proximal to the eastern valley wall. They are likely related to changes in the local stress field related to the obliquely trending eastern axial valley wall. Asymmetric character, such as nonuniform spacing and throw of faults, was not observed. These observations suggest that the asymmetry of the basin is not due to asymmetric spreading, but rather to eastward ridge jumps of several kilometers. Such small-amplitude ridge jumps likely occurred frequently during basin development because the asymmetric character of the basin is strongly pronounced and no abandoned rift valley is recognizable within the entire basi

High-resolution magnetic signature of active hydrothermal systems in the back-arc spreading region of the southern Mariana Trough

International audienceHigh-resolution vector magnetic measurements were performed on five hydrothermal vent fields of the back-arc spreading region of the southern Mariana Trough using Shinkai 6500, a deep-sea manned submersible. A new 3-D forward scheme was applied that exploits the surrounding bathymetry and varying altitudes of the submersible to estimate absolute crustal magnetization. The results revealed that magnetic-anomaly-derived absolute magnetizations show a reasonable correlation with natural remanent magnetizations of rock samples collected from the seafloor of the same region. The distribution of magnetic-anomaly-derived absolute magnetization suggests that all five andesite-hosted hydrothermal fields are associated with a lack of magnetization, as is generally observed at basalt-hosted hydrothermal sites. Furthermore, both the Pika and Urashima sites were found to have their own distinct low-magnetization zones, which could not be distinguished in magnetic anomaly data collected at higher altitudes by autonomous underwater vehicle due to their limited extension. The spatial extent of the resulting low magnetization is approximately 10 times wider at off-axis sites than at on-axis sites, possibly reflecting larger accumulations of nonmagnetic sulfides, stockwork zones, and/or alteration zones at the off-axis sites

Eicosapentaenoic Acid Intake Associated with Reduced Risk of Posttraumatic Stress Disorder after the Great East Japan Earthquake and Tsunami

Posttraumatic stress disorder (PTSD) is a debilitating condition characterized by intrusion, avoidance, hyperarousal symptoms after exposure to traumatic events. Since polyunsaturated fatty acids (PUFAs) have been implicated, we examined the possible association of PTSD with plasma PUFA level and dietary fish intake in 563 women who was struck by the Great East Japan Earthquake and Tsunami. The impact event scale-revised (IES-R) was used to assess PTSD symptoms. Dietary intake was estimated by a self-report questionnaire. Multivariate analysis controlling for age, body mass index, and stress revealed that PTSD status (IES-R ≥ 25) was associated with plasma eicosapentaenoic acid (EPA) level (P = 0.039). In the high-stress group, there were significantly inverse correlations of plasma EPA with IES-R total (r = −0.389, P = 0.031), intrusion (r = −0.370, P = 0.04), and hyperarousal scores (r = −0.480, P = 0.006), although such correlations were not found in the moderate-stress group. Fish intake that increased plasma EPA showed similar correlations with IES-R scores in the severely stressed group. Our results suggest that higher plasma EPA level and EPA-increasing fish intake are associated with a lower risk for PTSD in individuals who have suffered severe stress in a natural disaster

本邦で分離されたカルバペネマーゼ産生肺炎桿菌の分子遺伝学的解析

Carbapenemase-producing Enterobacteriaceae represent a serious public health threat worldwide. Carbapenemase genes, harbored on a transferable plasmid, have been isolated globally with distinct geographical features. Klebsiella pneumoniae, included in Enterobacteriaceae, also produces carbapenemase and often shows hypervirulence. Overlapping carbapenem resistance and hypervirulence in K. pneumoniae have been reported, but such strains have not yet been found in Japan. Here, we screened 104 carbapenemase-producing K. pneumoniae isolates collected from 37 hospitals and outpatient clinics in Japan between September 2014 and July 2015. PCR and DNA sequencing demonstrated IMP-1 in 21 isolates and IMP-6 in 83 isolates, 77 of which coharbored CTX-M-2. Most of the isolates showed low MICs toward imipenem and meropenem but high MICs toward penicillin and cephalosporins. Conjugation experiments with an Escherichia coli J53 recipient showed that most of the plasmids in IMP-6 producers were transferable, whereas only one-half of the plasmids in IMP-1 producers were transferable. PCR-based replicon typing and multiplex PCR identified five isolates belonging to the CG258 non-tonB79 cluster and no isolate belonging to the CG258-tonB79 cluster or sequence type 307 (ST307). Four K1-ST23 isolates, 10 K2-ST65 isolates, and 7 K2-ST86 isolates were detected that harbored virulence genes. The resistance genes in 85 isolates were transferable, but the virulence genes were not transferred. These results demonstrate the acquisition of IMP-type carbapenemase genes and CTX-M-type genes among hypervirulence isolates in Japan, warranting further attention and countermeasures. In this study, we have determined the molecular characteristics and epidemiology of IMP-6 producers that coharbored various CTX-M genes in Japan.IMPORTANCE Carbapenems serve as a last resort for the clinical treatment of multidrug-resistant infections. Therefore, the rapid spread of carbapenemase-producing strains represents a serious public health threat, further limiting antibiotic choices. The current findings of hypervirulent carbapenemase-producing Klebsiella pneumoniae clinical isolates in Japan demonstrate the potential broad spread and transfer of these genes, necessitating close surveillance.博士（医学）・乙第1509号・令和3年3月15日Copyright © 2020 Yonekawa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license(https://creativecommons.org/licenses/by/4.0/)

Validation of Plasmodium vivax centromere and promoter activities using Plasmodium yoelii

Plasmodium vivax is the leading cause of malaria outside Africa and represents a significant health and economic burden on affected countries. A major obstacle for P. vivax eradication is the dormant hypnozoite liver stage that causes relapse infections and the limited antimalarial drugs that clear this stage. Advances in studying the hypnozoite and other unique biological aspects of this parasite are hampered by the lack of a continuous in vitro laboratory culture system and poor availability of molecular tools for genetic manipulation. In this study, we aim to develop molecular tools that can be used for genetic manipulation of P. vivax. A putative P. vivax centromere sequence (PvCEN) was cloned and episomal centromere based plasmids expressing a GFP marker were constructed. Centromere activity was evaluated using a rodent malaria parasite Plasmodium yoelii. A plasmid carrying PvCEN was stably maintained in asexual-stage parasites in the absence of drug pressure, and approximately 45% of the parasites retained the plasmid four weeks later. The same retention rate was observed in parasites possessing a native P. yoelii centromere (PyCEN)-based control plasmid. The segregation efficiency of the plasmid per nuclear division was > 99% in PvCEN parasites, compared to ?90% in a control parasite harboring a plasmid without a centromere. In addition, we observed a clear GFP signal in both oocysts and salivary gland sporozoites isolated from mosquitoes. In blood-stage parasites after liver stage development, GFP positivity in PvCEN parasites was comparable to control PyCEN parasites. Thus, PvCEN plasmids were maintained throughout the parasite life cycle. We also validated several P. vivax promoter activities and showed that hsp70 promoter (?1 kb) was active throughout the parasite life cycle. This is the first data for the functional characterization of a P. vivax centromere that can be used in future P. vivax biological research

Faulting and volcanism in the axial valley of the slow-spreading center of the Mariana back arc basin from Wadatsumi side-scan sonar images

Author Posting. © American Geophysical Union, 2005. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry Geophysics Geosystems 6 (2005): Q05006, doi:10.1029/2004GC000881.We analyzed in detail the geology of the median valley floor of the Mariana Basin slow-spreading ridge using sea surface geophysical data and a high-resolution deep-tow side-scan sonar survey over one spreading segment. Analysis of surface magnetic data indicates highly asymmetric accretion, with the half-spreading rate on the western side of the basin being two to three times larger than on the eastern side. Surface magnetic and reflectivity data together suggest that asymmetric spreading is accomplished through eastward ridge jumps of ∼10 km of amplitude. Deep-tow backscatter data indicate along-axis variations of the volcanic processes with the emplacement of smooth and hummocky flows at the segment center and end, respectively. This variation likely relates to changes in the effusion rate due to the deepening or even disappearance of the magma chamber toward the segment end. Concerning tectonic processes, we find a power law distribution of the fractures, with an exponent of 1.74. This suggests that within the inner valley floor, fracture growth prevails over fracture nucleation and coalescence and that fractures accommodate less than 8% of the strain. According to our calculation based on a ratio of 0.02 to 0.03 between the vertical displacement and the length of faults, the amount of tectonic strain accommodated in the inner valley floor would consistently be ∼1.1–3.4%. Data also show two distinct sets of fractures. One trend is parallel to the rift direction at the segment center (∼N160°E) and perpendicular to the plate separation direction. Another set trends ∼17° oblique to this direction (∼N175°E) and is located over the eastern part of the valley, in the vicinity of a major bounding fault also trending ∼N175°E, that is, obliquely to the direction of plate motion. We modeled the stress field near a major fault that is oblique to the regional stress field associated with plate separation using a three-dimensional boundary element approach. We found that the orientation of the predicted fissuring near the oblique fault is locally rotated by ∼15° due to a flexure of the bending plate close to this fault.The KR03-12 cruise was funded by both JAMSTEC and ORI. This research was supported by the Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution, with funding provided by the United States Geological Survey

Plasmodium knowlesi thioredoxin peroxidase 1 binds to nucleic acids and has RNA chaperone activity

Malaria parasites are under oxidative attack throughout their life cycle in human body and mosquito vector. Therefore, Plasmodium antioxidant defenses are crucial for its survival and being considered as interesting target for antimalarial drug design. Plasmodium knowlesi has emerged recently from its simian host to human in Southeast Asia and has been recognized as the fifth Plasmodium species that can cause human malaria. In this study, we cloned and characterized thioredoxin peroxidase 1 from P. knowlesi (PkTPx-1). PkTPx-1 gene was cloned, and recombinant protein was produced by heterologous overexpression in Escherichia coli. The recombinant protein was used for evaluation of enzymatic activity and polyclonal antibody production. Using the recombinant PkTPx-1 protein, its antioxidant activity was confirmed in a mixed-function oxidation assay where PkTPx-1 prevented nicking of DNA by hydroxyl radicals. PkTPx-1 was able to bind to double-strand DNA and RNA and had RNA chaperone activity in a nucleic acid melting assay indicating new function of PkTPx-1 other than antioxidant activity. Using specific polyclonal antibodies, it was indicated that PkTPx-1 is expressed in the cytoplasm of the parasite. Altogether, these results suggest that PkTPx-1 not only protects the parasite from the adverse effects of reactive oxygen species but also has RNA chaperone activity

Displacement Transition in CH/Cyclohexane Adsorbed on Graphite

The adsorption of methane on cyclohexane-preplated graphite has been studied volumetrically at 77 K. Methane undergoes a displacement transition as well as layer-by-layer condensation transitions, similarly to Kr studied previously. The isotherm step of the displacement transition is clear and sharp, being indicative of a first-order phase transition. The transition takes place when the adsorbed methane molecules exceed the pre-adsorbed cyclohexane in number. The results support our previous interpretation that the displacement transition is a change from the layer-on-layer mode to the two-phase mode of two-component adsorption

R/V Yokosuka Cruise Report YK18-05

調査海域: 四国沖, 日向灘 / Area: Off Shikoku, Hyuga-Nada ; 期間: 2018年4月29日～2018年5月3日 / Operation Period: April 29, 2018～May 3, 2018http://www.godac.jamstec.go.jp/darwin/cruise/yokosuka/yk18-05/