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On-chip Acousto Thermal Shift Assay for Rapid and Sensitive Assessment of Protein Thermodynamic Stability
In article number 2003506, Xiaoyun Ding and co‐workers present an acousto thermal shift assay (ATSA) utilizing acoustic mechanisms to denature proteins and concentrate protein precipitates for rapid and label‐free assessment of protein thermodynamic changes in real time. The whole process, including sample handling, protein unfolding, and measurement, takes place within a microfluidic chip in less than two minutes, and is faster than other current thermal shift assays.</p
Comparison of ubiquinol and cytochrome c terminal oxidases An alternative view
AbstractThere have been numerous instances in the recent literature where the properties of ubiquinol and cytochrome c terminal oxidases are compared. Here we specifically examine the cytochrome bo3-type ubiquinol oxidase from Escherichia coli and the cytochrome aa3-type cytochrome c oxidases. A second redox-active copper site (CuA) is present only in the cytochrome c oxidases and the physiological electron donors for the two enzymes are different (ubiquinol-8 vs. ferrocytochrome c). In our opinion, these differences are significant and most likely indicate that distinct turnover mechanisms are operative in the two enzymes