Landscape at Collioure

The painting is of an abstract landscape in hues of blue, red, yellow, green, purple, and orange

Landscape at Collioure

full vie

Maladie coeliaque, dernières données sur la génétique (avancées, perspectives)

LYON1-BU Santé (693882101) / SudocRENNES1-BU Santé (352382103) / SudocSudocFranceF

miR-92a: A Novel Potential Biomarker of Rapid Aortic Valve Calcification

International audienceBackground and aim of the study: The study aim wasto compare the tissular expression of microRNAs(miRs) in bicuspid and tricuspid valves, and to evaluate their use as potential novel biomarkers of aortic valve calcification in bicuspid valves. Methods: A prospective single-center observational study was conducted on stenotic bicuspid andtricuspid human aortic valves. According to their potential role in valve vascular and valvular calcification, a decision was taken to include miR-92a, miR-141, and miR-223 in this analysis. A real-time quantitative polymerase chain reaction was used to measure the expression of each miR, using U6and Cel-miR-39 as endogenous and exogenous genecontrols, respectively. Results: Among a total of 47 human calcified aortic valves collected, 30 (63.8%) were tricuspid valves. Theme an preoperative transvalvular gradient was 50.8mmHg (range: 37-89 mmHg), with no significant difference between bicuspid and tricuspid valves(50 mmHg versus 51.2 mmHg; p = 0.729). Theme an aortic valve area was 0.79 cm2 (range: 0.33-1.3 cm2), again with no significant difference between the two groups (p = 0.34). The level of miR-92a expression was twofold higher in bicuspid valves compared to tricuspid valves (0.38 versus 0.17; p = 0.016), butno significant difference in miR-141 and miR-223 expression was observed between the two groups (p =0.68 and p = 0.35, respectively). A positive correlation was observed between miR-92a expression and mean preoperative transvalvular gradient (r = 0.3257, p =0.04). Conclusion: miR-92a is overexpressed in calcified bicuspid aortic valves, and may serve as a potential biomarker of rapid aortic valve calcification. Further studies based on these results may be designed to correlate the relative expression of miR-92a in the serum with its tissular expression in AS

Indoxyl sulfate impairs erythropoiesis at BFU-E stage in chronic kidney disease

Chronic kidney disease (CKD) is a global health condition characterized by a progressive deterioration of kidney function. It is associated with high serum levels of uremic toxins (UT), such as Indoxyl Sulfate (IS), which may participate in the genesis of several uremic complications. Anemia is one of the major complications in CKD patients that contribute to cardiovascular disease, increase morbi-mortality, and is associated with a deterioration of kidney failure in these patients. Our study aimed to characterize the impact of IS on CKD-related erythropoiesis. Using cellular and pre-clinical models, we studied cellular and molecular effects of IS on the growth and differentiation of erythroid cells. First, we examined the effect of clinically relevant concentrations of IS (up to 250 μM) in the UT7/EPO cell line. IS at 250 μM increased apoptosis of UT7/EPO cells at 48 h compared to the control condition. We confirmed this apoptotic effect of IS in erythropoiesis in human primary CD34+ cells during the later stages of erythropoiesis. Then, in IS-treated human primary CD34+ cells and in a (5/6 Nx) mice model, a blockage at the burst-forming unit-erythroid (BFU-E) stage of erythropoiesis was also observed. Finally, IS deregulates a number of erythropoietic related genes such as GATA-1, Erythropoietin-Receptor (EPO-R), and β-globin. Our findings suggest that IS could affect cell viability and differentiation of erythroid progenitors by altering erythropoiesis and contributing to the development of anemia in CKD

Bilan du séquençage de collections de gènes exprimés des animaux d'élevage

National audienc

Unconscious integration of multisensory bodily inputs in the peripersonal space shapes bodily self-consciousness

International audienceRecent studies have highlighted the role of multisensory integration as a key mechanism of self-consciousness. In particular, integration of bodily signals within the peripersonal space (PPS) underlies the experience of the self in a body we own (self-identification) and that is experienced as occupying a specific location in space (self-location), two main components of bodily self-consciousness (BSC). Experiments investigating the effects of multisensory integration on BSC have typically employed supra-threshold sensory stimuli, neglecting the role of unconscious sensory signals in BSC, as tested in other consciousness research. Here, we used psychophysical techniques to test whether multisensory integration of bodily stimuli underlying BSC also occurs for multisensory inputs presented below the threshold of conscious perception. Our results indicate that visual stimuli rendered invisible through continuous flash suppression boost processing of tactile stimuli on the body (Exp. 1), and enhance the perception of near-threshold tactile stimuli (Exp. 2), only once they entered PPS. We then employed unconscious multisensory stimulation to manipulate BSC. Participants were presented with tactile stimulation on their body and with visual stimuli on a virtual body, seen at a distance, which were either visible or rendered invisible. We found that participants reported higher self-identification with the virtual body in the synchronous visuo-tactile stimulation (as compared to asynchronous stimulation; Exp. 3), and shifted their self-location toward the virtual body (Exp.4), even if stimuli were fully invisible. Our results indicate that multisensory inputs, even outside of awareness, are integrated and affect the phenomenological content of self-consciousness, grounding BSC firmly in the field of psychophysical consciousness studies