Development and evaluation of lessons for class and group situations in grade I. Volume I.

Thesis (Ed.M.)--Boston University For volume II, please see: http://hdl.handle.net/2144/1415

Testing Sunyaev-Zel'dovich measurements of the hot gas content of dark matter haloes using synthetic skies

The thermal Sunyaev-Zel'dovich (tSZ) effect offers a means of probing the hot gas in and around massive galaxies and galaxy groups and clusters, which is thought to constitute a large fraction of the baryon content of the Universe. The Planck collaboration recently performed a stacking analysis of a large sample of `locally brightest galaxies' (LBGs) and, surprisingly, inferred an approximately self-similar relation between the tSZ flux and halo mass. At face value, this implies that the hot gas mass fraction is independent of halo mass, a result which is in apparent conflict with resolved X-ray observations. We test the robustness of the inferred trend using synthetic tSZ maps generated from cosmological hydrodynamical simulations and using the same tools and assumptions applied in the Planck study. We show that, while the detection and the estimate of the `total' flux (within $5 r_{500}$) is reasonably robust, the inferred flux originating from within $r_{500}$ (i.e. the limiting radius to which X-ray observations typically probe) is highly sensitive to the assumed pressure distribution of the gas. Using our most realistic simulations with AGN feedback, that reproduce a wide variety of X-ray and optical properties of groups and clusters, we estimate that the derived tSZ flux within $r_{500}$ is biased high by up to to an order of magnitude for haloes with masses $M_{500} \sim 10^{13}$ M$_{\odot}$. Moreover, we show that the AGN simulations are consistent with the total tSZ flux-mass relation observed with Planck, whereas a self-similar model is ruled out.Comment: 16 pages, 10 figures, MNRAS, accepted after minor revisio

Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia

Aberrant placentation generating placental oxidative stress is proposed to play a critical role in the pathophysiology of preeclampsia. Unfortunately, therapeutic trials of antioxidants have been uniformly disappointing. There is provisional evidence implicating mitochondrial dysfunction as a source of oxidative stress in preeclampsia. Here we provide evidence that mitochondrial reactive oxygen species mediates endothelial dysfunction and establish that directly targeting mitochondrial scavenging may provide a protective role. Human umbilical vein endothelial cells exposed to 3% plasma from women with pregnancies complicated by preeclampsia resulted in a significant decrease in mitochondrial function with a subsequent significant increase in mitochondrial superoxide generation compared to cells exposed to plasma from women with uncomplicated pregnancies. Real-time PCR analysis showed increased expression of inflammatory markers TNF-α, TLR-9 and ICAM-1 respectively in endothelial cells treated with preeclampsia plasma. MitoTempo is a mitochondrial-targeted antioxidant, pre-treatment of cells with MitoTempo protected against hydrogen peroxide-induced cell death. Furthermore MitoTempo significantly reduced mitochondrial superoxide production in cells exposed to preeclampsia plasma by normalising mitochondrial metabolism. MitoTempo significantly altered the inflammatory profile of plasma treated cells. These novel data support a functional role for mitochondrial redox signaling in modulating the pathogenesis of preeclampsia and identifies mitochondrial-targeted antioxidants as potential therapeutic candidates

Forest Decline and Tree Mortality in a Southeastern Ohio Oak-Hickory Forest

Author Institution: Department of Environmental and Plant Biology, Ohio UniversityMany forests throughout the central Appalachians have been suspected, or observed, to be in declining health. Few studies have examined the vitality of southeastern Ohio's forests. The purpose of our study was to evaluate the health of a representative southeastern Ohio oak-hickory forest. Thirty-two 0.1 ha plots were sampled in August 1995 on the 550 ha Waterloo Wildlife Experiment Station (ODNR) in Athens County, OH. Data were collected for all living and dead stems with a DBH >10 cm (1,891 stems sampled). For living trees, a decline index (DI) was determined to evaluate the percentage of branch dieback, undersized leaves, and chlorosis. Dead trees were identified and categorized by mortality class (log vs. snag). Quercus spp. and Carya spp. accounted for 68% of all stems sampled (83% living). Among the live trees, only three species (Juglans nigra, Sassafras albidum, and Cornus florida) exhibited nonhealthy DI values. All other species were observed to be healthy or exhibiting only trace symptoms of decline. In contrast to decline, high mortality was observed for S. albidum (29.1%), Liriodendron tulipifera (21.7%), and Prunus serotina (21.6%). Quercus spp. (17.0%), and Carya spp. (16.2%) exhibited moderate mortality. Acer spp. had the highest vitality among the dominant species. Most of the observed patterns can be explained by successional dynamics and known pathogenic processes. Various incongruities emphasize the need for long-term studies of forest dynamics and forest health monitoring

Mitochondrial [dys]function; culprit in pre-eclampsia?

Mitochondria are extensively identified for their bioenergetic capacities; however, recently these metabolic hubs are increasingly being appreciated as critical regulators of numerous cellular signalling systems. Mitochondrial reactive oxygen species have evolved as a mode of cross-talk between mitochondrial function and physiological systems, to sustain equipoise and foster adaption to cellular stress. Redox signalling mediated by exaggerated mitochondrial-ROS (reactive oxygen species) has been incriminated in a plethora of disease pathologies. Excessive production of mitochondrial ROS is intrinsically linked to mitochondrial dysfunction. Furthermore, mitochondrial dysfunction is a key facilitator of oxidative stress, inflammation, apoptosis and metabolism. These are key pathogenic intermediaries of pre-eclampsia, hence we hypothesize that mitochondrial dysfunction is a pathogenic mediator of oxidative stress in the pathophysiology of pre-eclampsia. We hypothesize that mitochondrial-targeted antioxidants may restrain production of ROS-mediated deleterious redox signalling pathways. If our hypothesis proves correct, therapeutic strategies directly targeting mitochondrial superoxide scavenging should be actively pursued as they may alleviate maternal vascular dysfunction and dramatically improve maternal and fetal health worldwide

Rotational Spectroscopy of PAHs: Acenaphthene, Acenaphthylene and Fluorene

Pure rotational spectra of three polycyclic aromatic hydrocarbons - acenaphthene, acenaphthylene and fluorene - have been obtained by Fourier transform microwave spectroscopy of a molecular beam and subsequently by millimeter wave absorption spectroscopy for acenaphthene and fluorene. The data presented here will be useful for deep radio astronomical searches for PAHs employing large radio telecopes.Comment: 2 pages, 1 figure (uses iaus.sty), to appear in IAU Symposium No. 231, Astrochemistry - Recent Successes and Current Challenges, eds. D. C. Lis, G. A. Blake & E. Herbst (Cambridge Univ. Press

Search for anions in molecular sources: C4H- detection in L1527

We present the results of a search for the negative ion C4H- in various dark clouds, low mass star-forming regions and photon-dominated regions (PDRs). We have also searched for C6H-, C2H- and CN- in some of the sources. The millimeter-wave observations were carried out with the IRAM-30m telescope. We detect C4H-, through the J = 9-8 and J = 10-9 rotational transitions, in the low mass star-forming region L1527. We thus confirm the tentative detection of the J = 9-8 line recently reported toward this source. The [C4H-]/[C4H] ratio found is 0.011 %, which is slightly lower than the value observed in IRC +10216, 0.024 %, but above the 3 sigma upper limit we derive in TMC-1, < 0.0052 %. We have also derived an upper limit for the [C6H-]/[C6H] ratio in the Horsehead Nebula, and for various anion-to-neutral ratios in the observed sources. These results are compared with recent chemical models.Comment: 4 pages, 1 figure; accepted for A&A Letter

Dust-forming molecules in VY Canis Majoris (and Betelgeuse)

The formation of inorganic dust in circumstellar environments of evolved stars is poorly understood. Spectra of molecules thought to be most important for the nucleation, i.e. AlO, TiO, and TiO2, have been recently detected in the red supergiant VY CMa. These molecules are effectively formed in VY CMa and the observations suggest that non-equilibrium chemistry must be involved in their formation and nucleation into dust. In addition to exploring the recent observations of VY CMa, we briefly discuss the possibility of detecting these molecules in the dust-poor circumstellar environment of Betelgeuse.Comment: contribution to Betelgeuse Workshop 2012: "The physics of Red Supergiants: recent advances and open questions", 26-29 Nov 2012 Paris (France

Laboratory And Astronomical Detection Of The Negative Molecular Ion C3N-

The negative molecular ion C3N- has been detected at millimeter wavelengths in a low-pressure laboratory discharge, and then with frequencies derived from the laboratory data in the molecular envelope of IRC+10216. Spectroscopic constants derived from laboratory measurements of 12 transitions between 97 and 378 GHz allow the rotational spectrum to be calculated well into the submillimeter-wave band to 0.03 km s(-1) or better in equivalent radial velocity. Four transitions of C3N- were detected in IRC+10216 with the IRAM 30 m telescope at precisely the frequencies calculated from the laboratory measurements. The column density of C3N- is 0.5% that of C3N, or approximately 20 times greater than that of C4H- relative to C4H. The C3N- abundance in IRC+10216 is compared with a chemical model calculation by Petrie & Herbst. An upper limit in TMC-1 for C3N- relative to C3N (< 0.8%) and a limit for C4H- relative to C4H (< 0.004%) that is 5 times lower than that found in IRC+10216, were obtained from observations with the NRAO 100 m Green Bank Telescope (GBT). The fairly high concentration ofNRFKorean government MEST 2012R1A1A1014646, 2012M4A2026720Southeast Physics Network (SEP-Net)Science and Technology Facilities Council ST/F002858/1, ST/I000976/1Swedish Research Council 2009-4088U.S. NSF AST-0708176, AST-1009799NASA NNX07AH09G, NNG04G177G, NNX11AE09GChandra grant SAO TM8-9009XBiochemistr