307 research outputs found
Kajian Struktur Komunitas Fitoplankton Di Perairan Pantai Desa Tapak Kecamatan Tugu Kota Semarang
Fitoplankton merupakan biota tumbuhan yang bersifat autotrophic dan merupakan produsen primer di perairan. Perairan Pantai Desa Tapak dikelilingi oleh Perumahan penduduk, kawasan industri, dan aktivitas nelayan yang akan menyumbang pencemaran organik maupun pencemaran anorganik. Perairan ini mengalami peningkatan ketinggian permukaan sebagai akibat pasang tinggi rob, yang mengakibatkan penggenangan daerah pertambakan dan sebagian dari estuaria. Penggenangan tersebut berakibat kepada peningkatan percampuran unsur hara yang diduga akan mempengaruhi keberadaan dan struktur komunitas fitoplankton pada daerah tersebut. Penelitian ini bertujuan untuk mengetahui struktur komunitas fitoplankton yang meliputi kelimpahan, keanekaragaman, keseragaman, dan dominasi fitoplankton di Perairan Pantai Desa Tapak, Kecamatan Tugu, Semarang. Penelitian ini dilaksanakan pada Oktober - November 2013. Metode yang digunakan dalam penelitian ini adalah deskriptif eksploratif, pengumpulan data dengan Sample Survey Method. Sampel fitoplankton diambil secara horizontal dengan planktonnet 37 µm. Sejumlah 3 stasiun pengambilan sampel telah ditetapkan dengan metode pertimbangan (purposive sampling method). Hasil penelitian menunjukkan bahwa struktur komunitas fitoplankton di Perairan Pantai Desa Tapak tersusun atas 3 kelas fitoplankton yang terdiri dari 24 genus fitoplankton, yaitu kelas Bacillariophyceae (17 genus), kelas Dinophyceae (5 genus), dan Cyanophyceae (2 genus). Genus yang mempunyai kelimpahan tertinggi adalah Skeletonema, Thalassionema, Thalassiothrix, dan Pleurosigma dari kelas Bacillariophyceae. Kelimpahan fitoplankton rata-rata berkisar 25.426 – 34.100 sel/m3. Nilai keanekaragaman berkisar 1,68 – 2,05, dimana tertinggi pada stasiun II dan terendah pada stasiun III. Keseragaman berkisar 0,66 – 0,76, dimana tertinggi pada stasiun II yang diikuti dengan stasiun I dan stasiun III. Nilai dominansi berkisar 0,24 – 0,34, dimana tertinggi pada stasiun III dan diikuti stasiun I dan stasiun I
First observation of Cherenkov rings with a large area CsI-TGEM-based RICH prototype
We have built a RICH detector prototype consisting of a liquid C6F14 radiator
and six triple Thick Gaseous Electron Multipliers (TGEMs), each of them having
an active area of 10x10 cm2. One triple TGEM has been placed behind the liquid
radiator in order to detect the beam particles, whereas the other five have
been positioned around the central one at a distance to collect the Cherenkov
photons. The upstream electrode of each of the TGEM stacks has been coated with
a 0.4 micron thick CsI layer.
In this paper, we will present the results from a series of laboratory tests
with this prototype carried out using UV light, 6 keV photons from 55Fe and
electrons from 90Sr as well as recent results of tests with a beam of charged
pions where for the first time Cherenkov Ring images have been successfully
recorded with TGEM photodetectors. The achieved results prove the feasibility
of building a large area Cherenkov detector consisting of a matrix of TGEMs.Comment: Presented at the International Conference NDIP-11, Lyon,July201
Aberrant DNA hypermethylation of the ITIH5 tumor suppressor gene in acute myeloid leukemia
Epigenetic mechanisms such as DNA hypermethylation and modifications of histone amino acids are known to play an important role in the control of gene expression both in normal human development and tumorigenesis. Hypermethylation of CpG islands within promoter regions of tumor suppressor genes is associated with transcriptional inactivation and represents, in addition to genetic aberrations, an important mechanism of gene silencing in the pathogenesis of human cancer. Inter-α-trypsine inhibitors (ITIs) are a family of serine protease inhibitors consisting of one light chain (bikunin) and two heavy chains (ITI heavy chains, ITIHs). ITIHs stabilize the extracellular matrix (ECM) by interacting with hyaluronic acid, which is a major ECM component. Hypermethylation in the upstream region of the promoter-associated CpG island of ITIH5, the most recently described member of the ITIH family, has been previously detected in breast cancer and was associated with an adverse outcome. In this study, we determined the DNA methylation status of the promoter region near the transcription start site of the ITIH5 tumor suppressor gene in leukemia cell lines and primary samples from patients with acute myeloid leukemia (AML) as well as the potential use of demethylating agents to restore a demethylated state of the promoter. Aberrant ITIH5 promoter hypermethylation occurred in 15 of 104 (14.4%) diagnostic AML samples. There were no statistically significant correlations between the ITIH5 methylation status and clinical prognostic parameters. Our results indicate that aberrant ITIH5 promoter hypermethylation is a novel epigenetic event in AML
Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures
For the ex vivo expansion of CD34+ cells, culture conditions were optimized using cytokine cocktails and media change methods. In addition, static, orbital-shake, and stirred cultures were compared. After cultivation, total cell expansion, immunophenotypes, clonogenic ability, and metabolite concentration in media were analyzed. Optimized media change methods enhanced the number of total nucleated cells (TNCs) by 600-fold (from 104 to 6 × 106 cells) in static cultures. Furthermore, intermittent orbital-shake cultures gave the highest fold increase of TNCs and CD34+/CD38− cells. These results imply that proliferation of CD34+ cells in intermittent shake cultures was more efficient than that in static cultures under optimized culture conditions
Lowering the radioactivity of the photomultiplier tubes for the XENON1T dark matter experiment
The low-background, VUV-sensitive 3-inch diameter photomultiplier tube R11410
has been developed by Hamamatsu for dark matter direct detection experiments
using liquid xenon as the target material. We present the results from the
joint effort between the XENON collaboration and the Hamamatsu company to
produce a highly radio-pure photosensor (version R11410-21) for the XENON1T
dark matter experiment. After introducing the photosensor and its components,
we show the methods and results of the radioactive contamination measurements
of the individual materials employed in the photomultiplier production. We then
discuss the adopted strategies to reduce the radioactivity of the various PMT
versions. Finally, we detail the results from screening 216 tubes with
ultra-low background germanium detectors, as well as their implications for the
expected electronic and nuclear recoil background of the XENON1T experiment.Comment: 10 pages, 5 figure
Search for Event Rate Modulation in XENON100 Electronic Recoil Data
We have searched for periodic variations of the electronic recoil event rate
in the (2-6) keV energy range recorded between February 2011 and March 2012
with the XENON100 detector, adding up to 224.6 live days in total. Following a
detailed study to establish the stability of the detector and its background
contributions during this run, we performed an un-binned profile likelihood
analysis to identify any periodicity up to 500 days. We find a global
significance of less than 1 sigma for all periods suggesting no statistically
significant modulation in the data. While the local significance for an annual
modulation is 2.8 sigma, the analysis of a multiple-scatter control sample and
the phase of the modulation disfavor a dark matter interpretation. The
DAMA/LIBRA annual modulation interpreted as a dark matter signature with
axial-vector coupling of WIMPs to electrons is excluded at 4.8 sigma.Comment: 6 pages, 4 figure
Search for Two-Neutrino Double Electron Capture of Xe with XENON100
Two-neutrino double electron capture is a rare nuclear decay where two
electrons are simultaneously captured from the atomic shell. For Xe
this process has not yet been observed and its detection would provide a new
reference for nuclear matrix element calculations. We have conducted a search
for two-neutrino double electron capture from the K-shell of Xe using
7636 kgd of data from the XENON100 dark matter detector. Using a
Bayesian analysis we observed no significant excess above background, leading
to a lower 90 % credibility limit on the half-life
yr. We also evaluated the sensitivity of the XENON1T experiment, which is
currently being commissioned, and find a sensitivity of
yr after an exposure of 2 tyr.Comment: 6 pages, 4 figure
Removing krypton from xenon by cryogenic distillation to the ppq level
The XENON1T experiment aims for the direct detection of dark matter in a
cryostat filled with 3.3 tons of liquid xenon. In order to achieve the desired
sensitivity, the background induced by radioactive decays inside the detector
has to be sufficiently low. One major contributor is the -emitter
Kr which is an intrinsic contamination of the xenon. For the XENON1T
experiment a concentration of natural krypton in xenon Kr/Xe < 200
ppq (parts per quadrillion, 1 ppq = 10 mol/mol) is required. In this
work, the design of a novel cryogenic distillation column using the common
McCabe-Thiele approach is described. The system demonstrated a krypton
reduction factor of 6.410 with thermodynamic stability at process
speeds above 3 kg/h. The resulting concentration of Kr/Xe < 26 ppq
is the lowest ever achieved, almost one order of magnitude below the
requirements for XENON1T and even sufficient for future dark matter experiments
using liquid xenon, such as XENONnT and DARWIN
Asymmetric Cell Divisions Sustain Long-Term Hematopoiesis from Single-sorted Human Fetal Liver Cells
Hematopoietic stem cells (HSCs) in adult marrow are believed to be derived from fetal liver precursors. To study cell kinetics involved in long-term hematopoiesis, we studied single-sorted candidate HSCs from fetal liver that were cultured in the presence of a mixture of stimulatory cytokines. After 8–10 d, the number of cells in primary cultures varied from <100 to >10,000 cells. Single cells in slow growing colonies were recloned upon reaching a 100–200 cell stage. Strikingly, the number of cells in subclones varied widely again. These results are indicative of asymmetric divisions in primitive hematopoietic cells in which proliferative potential and cell cycle properties are unevenly distributed among daughter cells. The continuous generation of functional heterogeneity among the clonal progeny of HSCs is in support of intrinsic control of stem cell fate and provides a model for the long-term maintenance of hematopoiesis in vitro and in vivo
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