Editorial: Abiotic Stresses in Agroecology:A Challenge for Whole Plant Physiology

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Regional isoprenoid emission from Eucalyptus grandis forests in northeastern Argentina

Eucalyptus species are strong source of isoprenoid emission. The objective of the present study was to estimate isoprenoid emissions from Eucalyptus grandis forest crops in the Entre Rios province in the Mesopotamia region of Argentina. The emission rates of isoprene and monoterpenes were measured on individual leaves under; controlled environmental conditions. At 30°C and with a PPFD of 1000 μM m-2 s-1 the emission rate of isoprene emission was 12.5 ± 1.9 nM m-2 s-1 and monoterpenes 15.1 ± 3.1 nM m-2 s-1. Emission was significantly affected by leaf position and decreased significantly from East to West. The most abundant compound emitted was limonene, accounting for between 50 and 68% of the total monoterpene emission. The time course of the isoprene emission course showed its strong dependence on light. On sunny days 42% of the isoprene emission comes from top of the canopy and only 2% from bottom of the canopy. But on overcast days, the relative contribution of diffuse radiation may be larger and can exceed the contribution of direct radiation. A model procedure in a Geographic Information System was implemented to estimate isoprene emissions at a regional scale. A forest inventory, data from a meteorological station and leaf area indices derived from satellite data served as inputs for the model. For the Entre Rios province (78781 km2), the isoprene emission totals up to 39.5 t d-1 on a clear summer day. The methodology applied to estimate isoprenoid emissions on a regional scale contributes to the understanding of carbon exchange between biosphere and atmosphereInst. de Clima y AguaFil: Richter, Klaus. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; ArgentinaFil: Centritto, Mauro. National Research Council. Trees and Timber Institute; ItaliaFil: Di Bella, Carlos Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Posse Beaulieu, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentin

Partial Root-Zone Drying of Olive (Olea europaea var. "Chetoui") Induces Reduced Yield under Field Conditions

The productivity of olive trees in arid and semi-arid environments is closely linked to irrigation. It is necessary to improve the efficiency of irrigation techniques to optimise the amount of olive fruit produced in relation to the volume of water used. Partial root-zone drying (PRD) is a water saving irrigation technique that theoretically allows the production of a root-to-shoot signal that modifies the physiology of the above-ground parts of the plant; specifically reducing stomatal conductance (gs) and improving water use efficiency (WUE). Partial root-zone drying has been successfully applied under field conditions to woody and non-woody crops; yet the few previous trials with olive trees have produced contrasting results. Thirty year-old olive trees (Olea europaea ‘var. Chetoui’) in a Tunisian grove were exposed to four treatments from May to October for three-years: ‘control’ plants received 100% of the potential evapotranspirative demand (ETc) applied to the whole root-zone; ‘PRD100’ were supplied with an identical volume of water to the control plants alternated between halves of the root-zone every ten-days; ‘PRD50’ were given 50% of ETc to half of the root-system, and; ‘rain-fed’ plants received no supplementary irrigation. Allowing part of the root-zone to dry resulted in reduced vegetative growth and lower yield: PRD100 decreased yield by ~47% during productive years. During the less productive years of the alternate bearing cycle, irrigation had no effect on yield; this suggests that withholding of water during ‘off-years’ may enhance the effectiveness of irrigation over a two-year cycle. The amount and quality of oil within the olive fruit was unaffected by the irrigation treatment. Photosynthesis declined in the PRD50 and rain-fed trees due to greater diffusive limitations and reduced biochemical uptake of CO2. Stomatal conductance and the foliar concentration of abscisic acid (ABA) were not altered by PRD100 irrigation, which may indicate the absence of a hormonal root-to-shoot signal. Rain-fed and PRD50 treatments induced increased stem water potential and increased foliar concentrations of ABA, proline and soluble sugars. The stomata of the olive trees were relatively insensitive to super-ambient increases in [CO2] and higher [ABA]. These characteristics of ‘hydro-passive’ stomatal behaviour indicate that the ‘Chetoui’ variety of olive tree used in this study lacks the physiological responses required for the successful exploitation of PRD techniques to increase yield and water productivity. Alternative irrigation techniques such as partial deficit irrigation may be more suitable for ‘Chetoui’ olive production.This work was supported by the Ministero dell’Istruzione, dell’Università e della Ricerca of Italy: PRIN 2010–2011 “PRO-ROOT” and Progetto Premiale 2012 “Aqua”. MH acknowledges funding from a Marie Curie IEF (2010–275626). We are grateful to Dr Mohamed Ghrab (Olive Tree Institute) for technical assistance and scientific discussion. The comments of Georgios Koubouris (Hellenic Agricultural Organization) and two anonymous reviewers significantly improved this manuscript

Dissecting Adaptation Mechanisms to Contrasting Solar Irradiance in the Mediterranean Shrub Cistus incanus

Molecular mechanisms that are the base of the strategies adopted by Mediterranean plants to cope with the challenges imposed by limited or excessive solar radiation during the summer season have received limited attention. In our study, conducted on C. incanus plants growing in the shade or in full sunlight, we performed measurements of relevant physiological traits, such as leaf water potential, gas exchange and PSII photochemistry, RNA-Seq with de-novo assembly, and the analysis of differentially expressed genes. We also identified and quantified photosynthetic pigments, abscisic acid, and flavonoids. Here, we show major mechanisms regulating light perception and signaling which, in turn, sustain the shade avoidance syndrome displayed by the ‘sun loving’ C. incanus. We offer clear evidence of the detrimental effects of excessive light on both the assembly and the stability of PSII, and the activation of a suite of both repair and effective antioxidant mechanisms in sun-adapted leaves. For instance, our study supports the view of major antioxidant functions of zeaxanthin in sunny plants concomitantly challenged by severe drought stress. Finally, our study confirms the multiple functions served by flavonoids, both flavonols and flavanols, in the adaptive mechanisms of plants to the environmental pressures associated to Mediterranean climate

Opportunities and limitations of crop phenotyping in southern european countries

ReviewThe Mediterranean climate is characterized by hot dry summers and frequent droughts. Mediterranean crops are frequently subjected to high evapotranspiration demands, soil water deficits, high temperatures, and photo-oxidative stress. These conditions will become more severe due to global warming which poses major challenges to the sustainability of the agricultural sector in Mediterranean countries. Selection of crop varieties adapted to future climatic conditions and more tolerant to extreme climatic events is urgently required. Plant phenotyping is a crucial approach to address these challenges. High-throughput plant phenotyping (HTPP) helps to monitor the performance of improved genotypes and is one of the most effective strategies to improve the sustainability of agricultural production. In spite of the remarkable progress in basic knowledge and technology of plant phenotyping, there are still several practical, financial, and political constraints to implement HTPP approaches in field and controlled conditions across the Mediterranean. The European panorama of phenotyping is heterogeneous and integration of phenotyping data across different scales and translation of “phytotron research” to the field, and from model species to crops, remain major challenges. Moreover, solutions specifically tailored to Mediterranean agriculture (e.g., crops and environmental stresses) are in high demand, as the region is vulnerable to climate change and to desertification processes. The specific phenotyping requirements of Mediterranean crops have not yet been fully identified. The high cost of HTPP infrastructures is a major limiting factor, though the limited availability of skilled personnel may also impair its implementation in Mediterranean countries. We propose that the lack of suitable phenotyping infrastructures is hindering the development of new Mediterranean agricultural varieties and will negatively affect future competitiveness of the agricultural sector. We provide an overview of the heterogeneous panorama of phenotyping within Mediterranean countries, describing the state of the art of agricultural production, breeding initiatives, and phenotyping capabilities in five countries: Italy, Greece, Portugal, Spain, and Turkey. We characterize some of the main impediments for development of plant phenotyping in those countries and identify strategies to overcome barriers and maximize the benefits of phenotyping and modeling approaches to Mediterranean agriculture and related sustainabilityinfo:eu-repo/semantics/publishedVersio

Effects of single or combined water deficit and aphid attack on tomato volatile organic compound (VOC) emission and plant-plant communication

Plants release a broad spectrum of volatile organic compounds (VOCs). The composition of the released VOC blend is dependent on the physiological status and, consequently, is affected by biotic and abiotic stresses. Stress-related VOCs can be perceived by different organisms, including natural enemies of herbivores and neighboring plants. Here, the responses of tomato plants (emitters) to single or combined abiotic (water stress) and biotic (aphid attack) stresses, and the effect of VOC released by emitters on neighboring unstressed plants (receivers), have been investigated. Emissions of α-pinene and methyl salicylate from plants exposed to single or combined stress, and of camphene from plants exposed to water or combined stress were significantly higher than in unstressed plants. In receivers, only the release of methyl salicylate increased when companion emitters were stressed. The expression of genes related to VOC biosynthesis and plant defense responses was unaffected or declined in water-stressed emitters, and was generally higher in receivers than in emitters. The gene coding for methyl salicylate biosynthesis was particularly active in aphid-attacked emitters and in receivers that were conditioned by the infested emitters. In addition, VOCs emitted by stressed plants induce VOC emission in unstressed receivers, and this increases attraction of parasitic wasps, which may improve protection against aphid attacks under conditions of reduced water availability

Metabolic plasticity in the hygrophyte Moringa oleifera exposed to water stress

Over the past decades, introduction of many fast-growing hygrophilic, and economically valuable plants into xeric environments has occurred. However, production and even survival of these species may be threatened by harsh climatic conditions unless an effective physiological and metabolic plasticity is available. Moringa oleifera Lam., a multipurpose tree originating from humid sub-tropical regions of India, is widely cultivated in many arid countries because of its multiple uses. We tested whether M. oleifera can adjust primary and secondary metabolism to efficiently cope with increasing water stress. It is shown that M. oleifera possesses an effective isohydric behavior. Water stress induced a quick and strong stomatal closure, driven by abscisic acid (ABA) accumulation, and leading to photosynthesis inhibition with consequent negative effects on biomass production. However, photochemistry was not impaired and maximal fluorescence and saturating photosynthesis remained unaffected in stressed leaves. We report for the first time that M. oleifera produces isoprene, and show that isoprene emission increased three-fold during stress progression. It is proposed that higher isoprene biosynthesis helps leaves cope with water stress through its antioxidant or membrane stabilizing action, and also indicates a general MEP (methylerythritol 4-phosphate) pathway activation that further helps protect photosynthesis under water stress. Increased concentrations of antioxidant flavonoids were also observed in water stressed leaves, and probably cooperate in limiting irreversible effects of the stress in M. oleifera leaves. The observed metabolic and phenotypic plasticity may facilitate the establishment of M. oleifera in xeric environments, sustaining the economic and environmental value of this plant

Isoprene Responses and Functions in Plants Challenged by Environmental Pressures Associated to Climate Change

The functional reasons for isoprene emission are still a matter of hot debate. It was hypothesized that isoprene biosynthesis evolved as an ancestral mechanism in plants adapted to high water availability, to cope with transient and recurrent oxidative stresses during their water-to-land transition. There is a tight association between isoprene emission and species hygrophily, suggesting that isoprene emission may be a favorable trait to cope with occasional exposure to stresses in mesic environments. The suite of morpho-anatomical traits does not allow a conservative water use in hygrophilic mesophytes challenged by the environmental pressures imposed or exacerbated by drought and heat stress. There is evidence that in stressed plants the biosynthesis of isoprene is uncoupled from photosynthesis. Because the biosynthesis of isoprene is costly, the great investment of carbon and energy into isoprene must have relevant functional reasons. Isoprene is effective in preserving the integrity of thylakoid membranes, not only through direct interaction with their lipid acyl chains, but also by up-regulating proteins associated with photosynthetic complexes and enhancing the biosynthesis of relevant membrane components, such as mono- and di-galactosyl-diacyl glycerols and unsaturated fatty acids. Isoprene may additionally protect photosynthetic membranes by scavenging reactive oxygen species. Here we explore the mode of actions and the potential significance of isoprene in the response of hygrophilic plants when challenged by severe stress conditions associated to rapid climate change in temperate climates, with special emphasis to the concomitant effect of drought and heat. We suggest that isoprene emission may be not a good estimate for its biosynthesis and concentration in severely droughted leaves, being the internal concentration of isoprene the important trait for stress protection

Comparing actual transpiration fluxes as measured at leaf-scale and calculated by a physically based agro-hydrological model

The main purpose of this paper is to compare the actual transpiration rates from tomato crop, as measured at leaf scale and estimated by a macroscopic approach in an agro-hydrological model, named FLOWS-HAGES, under variable soil properties and water availability. To this aim, sixteen plots were cultivated with tomatoes in Metaponto, Southern Italy. Soil hydraulic properties (SHP) were obtained using a fast in-situ characterization method. Leaf-area index (LAI) was measured using a leaf-area meter. SHP and LAI were then used in the physically-based FLOWS-HAGES which allowed calculating the macroscopic transpiration rates, Ta,m. Single-leaf transpiration rates, Ta,l, and stomatal conductance, gs,l, were measured in situ. For comparing with Ta,m, gs,l was upscaled by Big-Leaf approach to canopy scale stomatal conductance, gs,c, which was applied to Penman-Monteith model to obtain the canopy-scale transpiration, Ta,c. Finally, multiple linear regression (MLR) was used to find the statistical relationship between Ta,m and Ta,c, and the SHP and gs,c. Results showed that the macroscopic approach smooths the spatial variability of transpiration rates. Ta,c increased with the saturated water content, θs, and the slope of the water retention curve, n, while Ta,m decreased with increasing θs and n. MLR improved significantly by introducing gs,c to predict Ta,m