The importance and direction of current and future plant-UV research : break-out session discussions at the UV4Plants Network Meeting in Bled (April 15th -18th , 2018)

During the 2nd Network Meeting of UV4Plants at Bled (14th–18th April, 2018) the delegates engaged in a group discussion of prescient questions concerning the future of in plant-UV research. The discussion group was tasked to identify the most valuable directions for plant UV research to take, and to create a coherent framework for how to move the field forward. Here, the outcome of these discussions is summarised in sections that follow the composition of discussion groups as ideas taken from a molecular, biochemical and physiological perspective followed by those from an ecological and plant production perspective. In each case, first basic research questions are considered and then applications and methodological considerations are put forward. Finally, some common ground bringing the two perspectives together is discussed, with the aim of solving scaling problems and ways in which the UV4Plants network might be put to good use.Peer reviewe

Evidence That Gene Activation and Silencing during Stem Cell Differentiation Requires a Transcriptionally Paused Intermediate State

A surprising portion of both mammalian and Drosophila genomes are transcriptionally paused, undergoing initiation without elongation. We tested the hypothesis that transcriptional pausing is an obligate transition state between definitive activation and silencing as human embryonic stem cells (hESCs) change state from pluripotency to mesoderm. Chromatin immunoprecipitation for trimethyl lysine 4 on histone H3 (ChIP-Chip) was used to analyze transcriptional initiation, and 3′ transcript arrays were used to determine transcript elongation. Pluripotent and mesodermal cells had equivalent fractions of the genome in active and paused transcriptional states (∼48% each), with ∼4% definitively silenced (neither initiation nor elongation). Differentiation to mesoderm changed the transcriptional state of 12% of the genome, with roughly equal numbers of genes moving toward activation or silencing. Interestingly, almost all loci (98–99%) changing transcriptional state do so either by entering or exiting the paused state. A majority of these transitions involve either loss of initiation, as genes specifying alternate lineages are archived, or gain of initiation, in anticipation of future full-length expression. The addition of chromatin dynamics permitted much earlier predictions of final cell fate compared to sole use of conventional transcript arrays. These findings indicate that the paused state may be the major transition state for genes changing expression during differentiation, and implicate control of transcriptional elongation as a key checkpoint in lineage specification

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Convective heat transfer performance of sand for thermal energy storage

This thesis seeks to examine the effective convective heat exchange of sand as a heat exchange medium. The goal of this exploratory research is to quantify the heat transfer coefficient of sand in a proposed Thermal Energy Storage (TES) system which intends to complement solar thermal power generation. Standard concentrator solar thermal power plants typically employ a heat transfer fluid (HTF) that is heated in the collector field then routed to the power generators or TES unit. A fairly clear option for a TES system would be to utilize the existing HTF as the working storage medium. However, the use of conventional HTF systems may be too expensive. These fluids are quite costly as the quantity needed for storage is high and for some fluids their associated high vapor pressures require expensive highly reinforced containment vessels. The proposed storage system seeks to use sand as the storage medium; greatly reducing the expenses involved for both medium and storage costs. Most prior TES designs using sand or other solids employed them in a fixed bed for thermal exchange. The proposed TES system will instead move the sand to drive a counter flow thermal exchange. This counter flow design allows for a much closer temperature of approach when compared to a fixed bed. As cost and performance are the primary goals to tackle of the proposed system, the evaluation of the sandâ s thermal exchange effectiveness in a flowing state is necessary. Experiments will be conducted to measure the effective heat transfer coefficient between the sand and representative solid surfaces used as the heat transfer conduits. Additional experiments that will be looked at are wear caused by the sand as a consideration for long term design viability as well as angle of repose of the sand and its effect on scoop design for improved materials handling. Key investigational aspects of these experiments involve the sand grain size as well as shape of the heat exchanger surfaces. The thesis will evaluate the resulting convective heat transfer coefficient of the sand as related to these features. The data will then be compared and verified with available literature of previously studied characteristic thermal properties of sand. The measured and confirmed data will then be used to further aid in a design model for the proposed TES system.M.S.Committee Chair: Jeter, Sheldon; Committee Member: Abdel-Khalik, Said; Committee Member: Ghiaasiaan, Seye

Pregnancy-induced chromatin remodeling in the breast of postmenopausal women.

Early pregnancy and multiparity are known to reduce the risk of women to develop breast cancer at menopause. Based on the knowledge that the differentiation of the breast induced by the hormones of pregnancy plays a major role in this protection, this work was performed with the purpose of identifying what differentiation-associated molecular changes persist in the breast until menopause. Core needle biopsies (CNB) obtained from the breast of 42 nulliparous (NP) and 71 parous (P) postmenopausal women were analyzed in morphology, immunocytochemistry and gene expression. Whereas in the NP breast, nuclei of epithelial cells were large and euchromatic, in the P breast they were small and hyperchromatic, showing strong methylation of histone 3 at lysine 9 and 27. Transcriptomic analysis performed using Affymetrix HG_U133 oligonucleotide arrays revealed that in CNB of the P breast, there were 267 upregulated probesets that comprised genes controlling chromatin organization, transcription regulation, splicing machinery, mRNA processing and noncoding elements including XIST. We concluded that the differentiation process induced by pregnancy is centered in chromatin remodeling and in the mRNA processing reactome, both of which emerge as important regulatory pathways. These are indicative of a safeguard step that maintains the fidelity of the transcription process, becoming the ultimate mechanism mediating the protection of the breast conferred by full-term pregnancy

Thermal Performance Evaluation of Two Thermal Energy Storage Tank Design Concepts for Use with a Solid Particle Receiver-Based Solar Power Tower

This paper presents the results of an extensive study of two thermal energy storage (TES) systems. The goal of the research is to make solar energy cost-competitive with other forms of electricity. A small-scale TES system was first built. The inner to outer layers were made of firebrick (FB), autoclaved aerated concrete (AAC) and reinforced concrete brick (CB). The experiments were conducted at temperatures of up to 1000 °C for sustained periods of time. AAC was found to be prone to cracking at temperatures exceeding 900 °C; as a result, AAC was eliminated from the second TES system. The second, larger-scale TES system was subsequently built of multiple layers of readily available materials, namely, insulating firebrick (IFB), perlite concrete (PC), expansion joint (EJ), and CB. All of the surfaces were instrumented with thermocouples to estimate the heat loss from the system. The temperature was maintained at approximately 800 °C to approximate steady state conditions closely. The steady state heat loss was determined to be approximately 4.4% for a day. The results indicate that high-temperature TES systems can be constructed of readily available materials while meeting the heat loss requirements for a falling particle receiver system, thereby contributing to reducing the overall cost of concentrating solar power systems

On-sun testing of an advanced falling particle receiver system

A 1 MWth high-temperature falling particle receiver was constructed and tested at the National Solar Thermal Test Facility at Sandia National Laboratories. The continuously recirculating system included a particle elevator, top and bottom hoppers, and a cavity receiver that comprised a staggered array of porous chevron-shaped mesh structures that slowed the particle flow through the concentrated solar flux. Initial tests were performed with a peak irradiance of ~300 kW/m2 and a particle mass flow rate of 3.3 kg/s. Peak particle temperatures reached over 700 °C near the center of the receiver, but the particle temperature increase near the sides was lower due to a non-uniform irradiance distribution. At a particle inlet temperature of ~440 °C, the particle temperature increase was 27 °C per meter of drop length, and the thermal efficiency was ~60% for an average irradiance of 110 kW/m2 . At an average irradiance of 211 kW/m2 , the particle temperature increase was 57.1 °C per meter of drop length, and the thermal efficiency was ~65%. Tests with higher irradiances are being performed and are expected to yield greater particle temperature increases and efficiencies