Experimental Evaluation of Design Procedures for Shear Strength of Deep Reinfoced Concrete Beams

In this paper, results from the monotonic testing of four reinforced concrete deep beams are presented. The behavior of the deep beams is described in terms of cracking pattern, load-versus-deflection response, failure mode, and strains in steel reinforcement and concrete. Despite different failure modes, the failure loads and corresponding ultimate deflections were similar in all four specimens. Yielding of both longitudinal and transverse reinforcement occurred prior to failure. Based on the test results, the shear design procedures contained in the ACI 318-99 Code and Appendix A of the ACI 318-02 Code were evaluated. Both design procedures yielded conservative predictions of the shear strength of the single-span deep beams

Relación entre los serotipos de lengua azul y su vector, en Europa y cuenca mediterránea

La Lengua Azul es una enfermedad de distribución mundial que afecta a rumiantes y se transmite a través de la picadura de las hembras del mosquito del Género Culicoides. Tradicionalmente, la enfermedad ha estado ligada a la presencia del mosquito Culicoides imicola, pero estudios recientes apuntan a una posible implicación de otras especies de mosquitos que puedan sobrevivir en regiones más frías. Utilizando un sistema de visualización geográfica ARCGIS 9.2 (ESRI®) se ha podido representar la distribución de las principales especies de Culicoides implicadas en la transmisión de Lengua Azul en Europa y norte de África y compararlo con el mapa de distribución de los serotipos del virus de la Lengua Azul. El análisis de los mapas realizados prueba que Culicoides imicola está relacionado con la transmisión de los serotipos 1, 2, 4, 9 y 16 y que el complejo Obsoletus transmite el serotipo 8 y podría ser capaz de transmitir otros serotipos del virus con los que hasta ahora no ha tenido contacto.Bluetongue (BT) is a worldwide spread disease affecting ruminants, which is transmitted by the biting of female midges from the Genus Culicoides. Traditionally, this disease has been linked with the presence of the midge Culicoides imicola, but, recent studies have suggested the possibility of other midge species being involved in the transmission of Bluetongue in cooler regions. By applying the geographic information system ARCGIS 9.2 (ESRI®), the distribution of the main Culicoides species involved in Blue Tongue transmission in Europe and the North of Africa and the distribution of BT serotypes in the same region has been represented. The analysis of the maps obtained shows that Culicoides imicola is involved in the transmission of Bluetongue serotypes 1, 2, 4, 9 and 16 and the Obsoletus complex could be able to transmit, besides BTV-8, other BT serotypes with which it has never been in contact so far

The Dynamics of the Pulmonary Microbiome During Mechanical Ventilation in the Intensive Care Unit and the Association with Occurrence of Pneumonia

RATIONALE: Ventilator-associated pneumonia (VAP) is the most common nosocomial infections in patients admitted to the ICU. The adapted island model predicts several changes in the respiratory microbiome during intubation and mechanical ventilation. OBJECTIVES: We hypothesised that mechanical ventilation and antibiotic administration decrease the diversity of the respiratory microbiome and that these changes are more profound in patients who develop VAP. METHODS: Intubated and mechanically ventilated ICU-patients were included. Tracheal aspirates were obtained three times a week. 16S rRNA gene sequencing with the Roche 454 platform was used to measure the composition of the respiratory microbiome. Associations were tested with linear mixed model analysis and principal coordinate analysis. MEASUREMENTS AND MAIN RESULTS: 111 tracheal aspirates were obtained from 35 patients; 11 had VAP, 18 did not have VAP. Six additional patients developed pneumonia within the first 48 hours after intubation. Duration of mechanical ventilation was associated with a decrease in α diversity (Shannon index; fixed-effect regression coefficient (β): -0.03 (95% CI -0.05 to -0.005)), but the administration of antibiotic therapy was not (fixed-effect β: 0.06; 95% CI -0.17 to 0.30). There was a significant difference in change of β diversity between patients who developed VAP and control patients for Bray-Curtis distances (p=0.03) and for Manhattan distances (p=0.04). Burkholderia, Bacillales and, to a lesser extent, Pseudomonadales positively correlated with the change in β diversity. CONCLUSION: Mechanical ventilation, but not antibiotic administration, was associated with changes in the respiratory microbiome. Dysbiosis of microbial communities in the respiratory tract was most profound in patients who developed VAP.info:eu-repo/semantics/publishedVersio

Centre-specific bacterial pathogen typing affects infection-control decision making

Whole-genome sequencing is becoming the de facto standard for bacterial outbreak surveillance and infection prevention. This is accompanied by a variety of bioinformatic tools and needs bioinformatics expertise for implementation. However, little is known about the concordance of reported outbreaks when using different bioinformatic workflows. In this multi-centre proficiency testing among 13 major Dutch healthcare-affiliated centres, bacterial whole-genome outbreak analysis was assessed. Centres who participated obtained two randomized bacterial datasets of Illumina sequences, a Klebsiella pneumoniae and a Vancomycin-resistant Enterococcus faecium, and were asked to apply their bioinformatic workflows. Centres reported back on antimicrobial resistance, multi-locus sequence typing (MLST), and outbreak clusters. The reported clusters were analysed using a method to compare landscapes of phylogenetic trees and calculating Kendall–Colijn distances. Furthermore, fasta files were analysed by state-of-the-art single nucleotide polymorphism (SNP) analysis to mitigate the differences introduced by each centre and determine standardized SNP cut-offs. Thirteen centres participated in this study. The reported outbreak clusters revealed discrepancies between centres, even when almost identical bioinformatic workflows were used. Due to stringent filtering, some centres failed to detect extended-spectrum beta-lactamase genes and MLST loci. Applying a standardized method to determine outbreak clusters on the reported de novo assemblies, did not result in uniformity of outbreak-cluster composition among centres

Influence of pH and Temperature on Struvite Purity and Recovery from Anaerobic Digestate

The precipitation of struvite (MgNH4PO4.6H2O) from wastewater streams simultaneosuly recovers nitrogen (N) and phosphorus (P) for reuse as fertilisers. Struvite crystallisation is controlled by pH, saturation index, temperature and other ions in the solution (e.g., Ca2+, Mg2+ and CO32−). This work studies the effect of pH and temperature on phosphorus and nitrogen removal via struvite precipitation and the quality of the resulting precipitate product (i.e., crystal size, morphology and purity). Struvite was precipitated in batch reactors from the supernatant produced during anaerobic sludge dewatering at a wastewater treatment works, under controlled pH (8, 9 and 10) and temperature (25, 33 and 40 °C) conditions. The optimal P removal as struvite, reduction of the co-precipitation with Ca and the increase in particle size of the struvite precipitates were determined. The results showed that temperatures of 33 °C and 40 °C are not recommended for struvite precipitation—i.e., at 33 °C the purity is lower, and at 40 °C the ammonia losses are induced by volatilisation. At all pH-tests, the P removal efficiency was >93%, but the highest phosphate content and purity as struvite were obtained at a pH of 9.0. The optimum pH and temperature for the formation of large crystals (84 µm) and a high purity (>70%) of the struvite precipitates were 9 and 25 °C, respectively

Strut-and-tie model based on crack band theory for deep beams

yesA simplified strut-and-tie model including size effect based on the crack band theory is proposed to evaluate the shear capacity of deep beams. Concrete struts are idealized as uniformly tapered prismatic members with a stress relief strip whereas horizontal and vertical shear reinforcements are assumed to be an internally statically indeterminate system. The shear transfer mechanism of concrete and shear reinforcement is then driven by using the energy equilibrium in the stress relief strip and crack band zone of concrete struts. The shear capacity predictions of deep beams obtained from the present models are in better agreement with 637 test results than those determined from strut-and-tie models proposed by ACI 318-08, EC-2, and Tan and Cheng. In addition, the trend of the shear capacity of deep beams against different parameters as predicted by the present models has a consistent agreement with that observed from experimental results. In particular, the present model shows that the normalized shear capacity of deep beams is proportional to (h)−0.25, where h = section overall depth

Application of Plasticity Theory to Reinforced Concrete Deep Beams

yesThis paper reviews the application of the plasticity theory to reinforced concrete deep beams. Both the truss analogy and mechanism approach were employed to predict the capacity of reinforced concrete deep beams. In addition, most current codes of practice, for example Eurocode 1992 and ACI 318-05, recommend the strut-and-tie model for designing reinforced concrete deep beams. Compared with methods based on empirical or semi-empirical equations, the strut-and-tie model and mechanism analyses are more rational, adequately accurate and sufficiently simple for estimating the load capacity of reinforced concrete deep beams. However, there is a problem of selecting the effectiveness factor of concrete as reflected in the wide range of values reported in the literature for deep beams