337 research outputs found
Topical photodynamic therapy using different porphyrin precursors leads to differences in vascular photosensitization and vascular damage in normal mouse skin
Different distributions of hexyl aminolevulinate (HAL), aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) in the superficial vasculature are not well studied but they are hypothesized to play an important role in topical photodynamic therapy (PDT). The colocalization of fluorescent CD31 and protoporphyrin IX (PpIX) was calculated using confocal microscopy of mouse skin sections to investigate the vascular distribution after topical application. Vascular damage leads to disruption of the normal endothelial adherens junction complex, of which CD144 is an integral component. Therefore, normal CD31 combined with loss of normal fluorescent CD144 staining was visually scored to assess vascular damage. Both the vascular PpIX concentration and the vascular damage were highest for HAL, then ALA and then MAL. Vascular damage in MAL was not different from normal contralateral control skin. This pattern is consistent with literature data on vasoconstriction after PDT, and with the hypothesis that the vasculature plays a role in light fractionation that increases efficacy for HAL and ALA-PDT but not for MAL. These findings indicate that endothelial cells of superficial blood vessels synthesize biologically relevant PpIX concentrations, leading to vascular damage. Such vascular effects are expected to influence the oxygenation of tissue after PDT which can be important for treatment efficacy. The ability of the vasculature to synthesize PpIX and be damaged by PDT was compared between HAL, ALA and MAL in mouse skin using confocal microscopy and fluorescent CD31 and CD144 antibodies. Colocalization of CD31 and PpIX (left images) was calculated to measure endothelial PpIX synthesis. Vascular damage was scored as loss of normal CD144 staining (right images). Both PpIX synthesis and vascular damage were highest for HAL, then ALA, then MAL. This illustrates that superficial blood vessels synthesize biologically relevant amounts of PpIX. Vascular responses can limit oxygen supply during or after PDT and are expected to influence outcome
The role of the interaction matrix in mean-field spin glasses
Mean-field models of 2-spin Ising spin glasses with interaction matrices
taken from ensembles which are invariant under O(N) transformations are
studied. A general study shows that the nature of the spin glass transition can
be deduced from the eigenvalue spectrum of the interaction matrix. A simple
replica approach is derived to carry out the average over the O(N) disorder.
The analytic results are confirmed by extensive Monte Carlo simulations for
large system sizes and by exact enumeration for small system sizes.Comment: 14 pages, 4 figure
Constraints on the CMB temperature redshift dependence from SZ and distance measurements
The relation between redshift and the CMB temperature,
is a key prediction of standard cosmology, but is violated in many non-standard
models. Constraining possible deviations to this law is an effective way to
test the CDM paradigm and search for hints of new physics. We present
state-of-the-art constraints, using both direct and indirect measurements. In
particular, we point out that in models where photons can be created or
destroyed, not only does the temperature-redshift relation change, but so does
the distance duality relation, and these departures from the standard behaviour
are related, providing us with an opportunity to improve constraints. We show
that current datasets limit possible deviations of the form
to be up to a redshift
. We also discuss how, with the next generation of space and
ground-based experiments, these constraints can be improved by more than one
order of magnitude.Comment: 27 pages, 11 figure
Muon Track Reconstruction and Data Selection Techniques in AMANDA
The Antarctic Muon And Neutrino Detector Array (AMANDA) is a high-energy
neutrino telescope operating at the geographic South Pole. It is a lattice of
photo-multiplier tubes buried deep in the polar ice between 1500m and 2000m.
The primary goal of this detector is to discover astrophysical sources of high
energy neutrinos. A high-energy muon neutrino coming through the earth from the
Northern Hemisphere can be identified by the secondary muon moving upward
through the detector. The muon tracks are reconstructed with a maximum
likelihood method. It models the arrival times and amplitudes of Cherenkov
photons registered by the photo-multipliers. This paper describes the different
methods of reconstruction, which have been successfully implemented within
AMANDA. Strategies for optimizing the reconstruction performance and rejecting
background are presented. For a typical analysis procedure the direction of
tracks are reconstructed with about 2 degree accuracy.Comment: 40 pages, 16 Postscript figures, uses elsart.st
Virtual Compton Scattering and Neutral Pion Electroproduction in the Resonance Region up to the Deep Inelastic Region at Backward Angles
We have made the first measurements of the virtual Compton scattering (VCS)
process via the H exclusive reaction in the nucleon resonance
region, at backward angles. Results are presented for the -dependence at
fixed GeV, and for the -dependence at fixed near 1.5 GeV.
The VCS data show resonant structures in the first and second resonance
regions. The observed -dependence is smooth. The measured ratio of
H to H cross sections emphasizes the different
sensitivity of these two reactions to the various nucleon resonances. Finally,
when compared to Real Compton Scattering (RCS) at high energy and large angles,
our VCS data at the highest (1.8-1.9 GeV) show a striking -
independence, which may suggest a transition to a perturbative scattering
mechanism at the quark level.Comment: 20 pages, 8 figures. To appear in Phys.Rev.
Acoustic phonon exchange, attractive interactions, and the Wentzel-Bardeen singularity in single-wall nanotubes
We derive the effective low-energy theory for interacting electrons in
metallic single-wall carbon nanotubes taking into account acoustic phonon
exchange within a continuum elastic description. In many cases, the nanotube
can be described as a standard Luttinger liquid with possibly attractive
interactions. We predict surprisingly strong attractive interactions for thin
nanotubes. Once the tube radius reaches a critical value \AA, the Wentzel-Bardeen singularity is approached, accompanied by strong
superconducting fluctuations. The surprisingly large indicates that this
singularity could be reached experimentally. We also discuss the conditions for
a Peierls transition due to acoustic phonons.Comment: 11 pages, 2 figures, final version to be published in Phys. Rev.
Metabolic diagnosis and medical prevention of calcium nephrolithiasis and its systemic manifestations: a consensus statement
Background: Recently published guidelines on the medical management of renal stone disease did not address relevant topics in the field of idiopathic calcium nephrolithiasis, which are important also for clinical research. Design: A steering committee identified 27 questions, which were proposed to a faculty of 44 experts in nephrolithiasis and allied fields. A systematic review of the literature was conducted and 5216 potentially relevant articles were selected; from these, 407 articles were deemed to provide useful scientific information. The Faculty, divided into working groups, analysed the relevant literature. Preliminary statements developed by each group were exhaustively discussed in plenary sessions and approved. Results: Statements were developed to inform clinicians on the identification of secondary forms of calcium nephrolithiasis and systemic complications; on the definition of idiopathic calcium nephrolithiasis; on the use of urinary tests of crystallization and of surgical observations during stone treatment in the management of these patients; on the identification of patients warranting preventive measures; on the role of fluid and nutritional measures and of drugs to prevent recurrent episodes of stones; and finally, on the cooperation between the urologist and nephrologist in the renal stone patients. Conclusions: This document has addressed idiopathic calcium nephrolithiasis from the perspective of a disease that can associate with systemic disorders, emphasizing the interplay needed between urologists and nephrologists. It is complementary to the American Urological Association and European Association of Urology guidelines. Future areas for research are identified
Dynamics of the O(e,e'p) cross section at high missing energies
We measured the cross section and response functions (R_L, R_T, and R_LT) for the 16O(e,e'p) reaction in quasielastic kinematics for missing energies 25 60 MeV and P_miss > 200 MeV/c, the cross section is relatively constant. Calculations which include contributions from pion exchange currents, isobar currents and short-range correlations account for the shape and the transversity but only for half of the magnitude of the measured cross section
Rotavirus group : a genotype circulation patterns across Kenya before and after nationwide vaccine introduction, 2010-2018
Background
Kenya introduced the monovalent G1P [8] RotarixÂź vaccine into the infant immunization schedule in July 2014. We examined trends in rotavirus group A (RVA) genotype distribution pre- (January 2010âJune 2014) and post- (July 2014âDecember 2018) RVA vaccine introduction.
Methods
Stool samples were collected from children aged <â13âyears from four surveillance sites across Kenya: Kilifi County Hospital, Tabitha Clinic Nairobi, Lwak Mission Hospital, and Siaya County Referral Hospital (children aged <â5âyears only). Samples were screened for RVA using enzyme linked immunosorbent assay (ELISA) and VP7 and VP4 genes sequenced to infer genotypes.
Results
We genotyped 614 samples in pre-vaccine and 261 in post-vaccine introduction periods. During the pre-vaccine introduction period, the most frequent RVA genotypes were G1P [8] (45.8%), G8P [4] (15.8%), G9P [8] (13.2%), G2P [4] (7.0%) and G3P [6] (3.1%). In the post-vaccine introduction period, the most frequent genotypes were G1P [8] (52.1%), G2P [4] (20.7%) and G3P [8] (16.1%). Predominant genotypes varied by year and site in both pre and post-vaccine periods. Temporal genotype patterns showed an increase in prevalence of vaccine heterotypic genotypes, such as the commonly DS-1-like G2P [4] (7.0 to 20.7%, P <â.001) and G3P [8] (1.3 to 16.1%, P <â.001) genotypes in the post-vaccine introduction period. Additionally, we observed a decline in prevalence of genotypes G8P [4] (15.8 to 0.4%, P <â.001) and G9P [8] (13.2 to 5.4%, P <â.001) in the post-vaccine introduction period. Phylogenetic analysis of genotype G1P [8], revealed circulation of strains of lineages G1-I, G1-II and P [8]-1, P [8]-III and P [8]-IV. Considerable genetic diversity was observed between the pre and post-vaccine strains, evidenced by distinct clusters.
Conclusion
Genotype prevalence varied from before to after vaccine introduction. Such observations emphasize the need for long-term surveillance to monitor vaccine impact. These changes may represent natural secular variation or possible immuno-epidemiological changes arising from the introduction of the vaccine. Full genome sequencing could provide insights into post-vaccine evolutionary pressures and antigenic diversity
Search for Neutrino-Induced Cascades with AMANDA
We report on a search for electro-magnetic and/or hadronic showers (cascades)
induced by high energy neutrinos in the data collected with the AMANDA II
detector during the year 2000. The observed event rates are consistent with the
expectations for atmospheric neutrinos and muons. We place upper limits on a
diffuse flux of extraterrestrial electron, tau and muon neutrinos. A flux of
neutrinos with a spectrum which consists of an equal mix
of all flavors, is limited to at
a 90% confidence level for a neutrino energy range 50 TeV to 5 PeV. We present
bounds for specific extraterrestrial neutrino flux predictions. Several of
these models are ruled out.Comment: 18 pages, 12 figure
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