Assessment of Buffet Forcing Function Development Process Using Unsteady Pressure Sensitive Paint

A wind tunnel test was conducted at the Ames Unitary Plan Wind Tunnel to characterize the transonic buffet environment of a generic launch vehicle forebody. The test examined a highly instrumented version of the Coe and Nute Model 11 test article first tested in the 1960s. One of the measurement techniques used during this test was unsteady pressure sensitive paint (uPSP) developed at the Arnold Engineering Development Complex. This optical measurement technique measured fluctuating pressures at over 300,000 locations on the surface of the model. The high spatial density of these measurements provided an opportunity to examine in depth the assumptions underpinning the development of buffet forcing functions (BFFs) used in the development of the Space Launch System vehicle. The comparison of discrete-measurement-based BFFs to BFFs developed by continuous surface pressure integration indicates that the current BFF development approach under predicts low frequency content of the BFFs while over predicting high frequency content. Coherence-based adjustments employed to reduce over prediction in the surface integration of discrete pressure measurements contribute to the inaccuracy of the BFFs and their implementation should be reevaluated

Ares Launch Vehicle Transonic Buffet Testing and Analysis Techniques

It is necessary to define the launch vehicle buffet loads to ensure that structural components and vehicle subsystems possess adequate strength, stress, and fatigue margins when the vehicle structural dynamic response to buffet forcing functions are considered. In order to obtain these forcing functions, the accepted method is to perform wind-tunnel testing of a rigid model instrumented with hundreds of unsteady pressure transducers designed to measure the buffet environment across the desired frequency range. The buffet wind-tunnel test program for the Ares Crew Launch Vehicle employed 3.5 percent scale rigid models of the Ares I and Ares I-X launch vehicles instrumented with 256 unsteady pressure transducers each. These models were tested at transonic conditions at the Transonic Dynamics Tunnel at NASA Langley Research Center. The ultimate deliverable of the Ares buffet test program are buffet forcing functions (BFFs) derived from integrating the measured fluctuating pressures on the rigid wind-tunnel models. These BFFs are then used as input to a multi-mode structural analysis to determine the vehicle response to buffet and the resulting buffet loads and accelerations. This paper discusses the development of the Ares I and I-X rigid buffet model test programs from the standpoint of model design, instrumentation system design, test implementation, data analysis techniques to yield final products, and presents normalized sectional buffet forcing function root-mean-squared levels

Analysis of a Transonic Alternating Flow Phenomenon Observed During Ares Crew Launch Vehicle Wind Tunnel Tests

A transonic wind tunnel test of the Ares I-X Rigid Buffet Model (RBM) identified a Mach number regime where unusually large buffet loads are present. A subsequent investigation identified the cause of these loads to be an alternating flow phenomenon at the Crew Module-Service Module junction. The conical design of the Ares I-X Crew Module and the cylindrical design of the Service Module exposes the vehicle to unsteady pressure loads due to the sudden transition from separated to attached flow about the cone-cylinder junction with increasing Mach number. For locally transonic conditions at this junction, the flow randomly fluctuates back and forth between a subsonic separated flow and a supersonic attached flow. These fluctuations produce a square-wave like pattern in the pressure time histories which, upon integration result in large amplitude, impulsive buffet loads. Subsequent testing of the Ares I RBM found much lower buffet loads since the evolved Ares I design includes an ogive fairing that covers the Crew Module-Service Module junction, thereby making the vehicle less susceptible to the onset of alternating flow. An analysis of the alternating flow separation and attachment phenomenon indicates that the phenomenon is most severe at low angles of attack and exacerbated by the presence of vehicle protuberances. A launch vehicle may experience either a single or, at most, a few impulsive loads since it is constantly accelerating during ascent rather than dwelling at constant flow conditions in a wind tunnel. A comparison of a wind-tunnel-test-data-derived impulsive load to flight-test-data-derived load indicates a significant over-prediction in the magnitude and duration of the buffet loa

Analysis of Ares Crew Launch Vehicle Transonic Alternating Flow Phenomenon

A transonic wind tunnel test of the Ares I-X Rigid Buffet Model (RBM) identified a Mach number regime where unusually large buffet loads are present. A subsequent investigation identified the cause of these loads to be an alternating flow phenomenon at the Crew Module-Service Module junction. The conical design of the Ares I-X Crew Module and the cylindrical design of the Service Module exposes the vehicle to unsteady pressure loads due to the sudden transition between a subsonic separated and a supersonic attached flow about the cone-cylinder junction as the local flow randomly fluctuates back and forth between the two flow states. These fluctuations produce a square-wave like pattern in the pressure time histories resulting in large amplitude, impulsive buffet loads. Subsequent testing of the Ares I RBM found much lower buffet loads since the evolved Ares I design includes an ogive fairing that covers the Crew Module-Service Module junction, thereby making the vehicle less susceptible to the onset of alternating flow. An analysis of the alternating flow separation and attachment phenomenon indicates that the phenomenon is most severe at low angles of attack and exacerbated by the presence of vehicle protuberances. A launch vehicle may experience either a single or, at most, a few impulsive loads since it is constantly accelerating during ascent rather than dwelling at constant flow conditions in a wind tunnel. A comparison of a windtunnel- test-data-derived impulsive load to flight-test-data-derived load indicates a significant over-prediction in the magnitude and duration of the buffet load. I. Introduction On

Effect of Surface Pressure Integration Methodology on Launch Vehicle Buffet Forcing Functions

The 2014 test of the Space Launch System (SLS) Rigid Buffet Model conducted at the NASA Langley Transonic Dynamics Tunnel employed an extremely high number of unsteady pressure transducers. The high channel count provided an opportunity to examine the effect of transducer placement on the resulting buffet forcing functions (BFFs). Rings of transducers on the forward half of the model were employed to simulate a single-body vehicle. The impact of transducer density, circumferential distribution, and loss of a single transducer on the resulting BFFs were examined. Rings of transducers on the aft half of the SLS model were employed to examine the effect of transducer density and circumferential distribution on BFFs for a multi-body configuration. Transducer placement considerations with respect to model size, facility infrastructure, and data acquisition system capabilities, which affect the integration process, are also discussed

Increased compensatory kidney workload results in cellular damage in a short time porcine model of mixed acidemia – Is acidemia a ‘first hit’ in acute kidney injury?

Acute kidney injury (AKI) corrupts the outcome of about 50% of all critically ill patients. We investigated the possible contribution of the pathology acidemia on the development of AKI. Pigs were exposed to acidemia, acidemia plus hypoxemia or a normal acid-base balance in an experimental setup, which included mechanical ventilation and renal replacement therapy to facilitate biotrauma caused by extracorporeal therapies. Interestingly, extensive histomorphological changes like a tubular loss of cell barriers occurred in the kidneys after just 5 hours exposure to acidemia. The additional exposure to hypoxemia aggravated these findings. These 'early' microscopic pathologies opposed intra vitam data of kidney function. They did not mirror cellular or systemic patterns of proinflammatory molecules (like TNF-α or IL 18) nor were they detectable by new, sensitive markers of AKI like Neutrophil gelatinase-associated lipocalin. Instead, the data suggest that the increased renal proton excretion during acidemia could be an 'early' first hit in the multifactorial pathogenesis of AKI

Pretransplant HLA typing revealed loss of heterozygosity in the major histocompatibility complex in a patient with acute myeloid leukemia

Introduction Chromosomal abnormalities are frequent events in hematological malignancies. The degree of HLA compatibility between donor and recipient in hematopoietic stem cell transplantation is critical. Purpose of the study In this report, we describe an acute myeloid leukemia case with loss of heterozygosity (LOH) encompassing the entire HLA. Materials and methods HLA molecular typing was performed on peripheral blood (PB) and buccal swabs (BS). Chromosomal microarray analysis (CMA) was performed using a whole genome platform. Results Typing results on PB sample collected during blast crisis demonstrated homozygosity at the -A, -B, -C, -DR, and -DQ loci. A BS sample demonstrated heterozygosity at all loci. A subsequent PB sample drawn after count recovery confirmed heterozygosity. The CMA performed on PB samples collected during and after blast crisis revealed a large terminal region of copy-neutral LOH involving chromosome region 6p25.3p21.31, spanning approximately 35.9 Mb. The results of the CMA assay on sample collected after count recovery did not demonstrate LOH. Conclusions LOH at the HLA gene locus may significantly influence the donor search resulting in mistakenly choosing homozygous donors. We recommend confirming the HLA typing of recipients with hematological malignancies when homozygosity is detected at any locus by using BS samples, or alternatively from PB when remission is achieved

Overview of the Space Launch System Transonic Buffet Environment Test Program

Fluctuating aerodynamic loads are a significant concern for the structural design of a launch vehicle, particularly while traversing the transonic flight environment. At these trajectory conditions, unsteady aerodynamic pressures can excite the vehicle dynamic modes of vibration and result in high structural bending moments and vibratory environments. To ensure that vehicle structural components and subsystems possess adequate strength, stress, and fatigue margins in the presence of buffet and other environments, buffet forcing functions are required to conduct the coupled load analysis of the launch vehicle. The accepted method to obtain these buffet forcing functions is to perform wind-tunnel testing of a rigid model that is heavily instrumented with unsteady pressure transducers designed to measure the buffet environment within the desired frequency range. Two wind-tunnel tests of a 3 percent scale rigid buffet model have been conducted at the Langley Research Center Transonic Dynamics Tunnel (TDT) as part of the Space Launch System (SLS) buffet test program. The SLS buffet models have been instrumented with as many as 472 unsteady pressure transducers to resolve the buffet forcing functions of this multi-body configuration through integration of the individual pressure time histories. This paper will discuss test program development, instrumentation, data acquisition, test implementation, data analysis techniques, and several methods explored to mitigate high buffet environment encountered during the test program. Preliminary buffet environments will be presented and compared using normalized sectional buffet forcing function root-meansquared levels along the vehicle centerline

Utility of an attention-based performance validity test for the detection of feigned cognitive dysfunction after acquired brain injury

Introduction: The Groningen Effort Test (GET) is a recently developed performance validity test (PVT) for the identification of noncredible performance in a neuropsychological assessment of attention abilities. Because the majority of already established PVTs are based on memory functions, the GET has the potential to make a valuable contribution to validity testing.Method: The current study examined the utility of the GET in the detection of feigned cognitive dysfunction after acquired brain injury (ABI) and its incremental validity over already established PVTs, namely the Test of Memory Malingering (TOMM), the Dot Counting Test (DCT), and the b Test. Three hundred and forty-eight participants took part in this study, including 58 patients with ABI (stroke or traumatic brain injury), 43 healthy individuals instructed to show normal behavior, and 247 healthy individuals instructed to feign cognitive dysfunction after ABI.Results: With excellent overall classification accuracy, the GET performed close to the level of the TOMM, and superior to the b Test and DCT. Data analyses further revealed that the GET provides additional diagnostic accuracy compared to the b Test and the DCT in the detection of feigned cognitive dysfunction, but has no incremental validity over the TOMM. For each of the four PVTs in this study, diagnostic sensitivity was independent of the simulation strategy used.Conclusions: It is concluded that the GET is an attention-based PVT with promising test characteristics and high diagnostic accuracy in the detection of noncredible cognitive performance using a simulation design. Given the results can be replicated in studies using known-groups methodology, it may be a useful tool for clinical practice to complement neuropsychological assessments of patients with ABI

Systematic interaction network filtering identifies CRMP1 as a novel suppressor of huntingtin misfolding and neurotoxicity

Assemblies of huntingtin (HTT) fragments with expanded polyglutamine (polyQ) tracts are a pathological hallmark of Huntington's disease (HD). The molecular mechanisms by which these structures are formed and cause neuronal dysfunction and toxicity are poorly understood. Here, we utilized available gene expression data sets of selected brain regions of HD patients and controls for systematic interaction network filtering in order to predict disease-relevant, brain region-specific HTT interaction partners. Starting from a large protein-protein interaction (PPI) data set, a step-by-step computational filtering strategy facilitated the generation of a focused PPI network that directly or indirectly connects 13 proteins potentially dysregulated in HD with the disease protein HTT. This network enabled the discovery of the neuron-specific protein CRMP1 that targets aggregation-prone, N-terminal HTT fragments and suppresses their spontaneous self-assembly into proteotoxic structures in various models of HD. Experimental validation indicates that our network filtering procedure provides a simple but powerful strategy to identify disease-relevant proteins that influence misfolding and aggregation of polyQ disease proteins.DFG [SFB740, 740/2-11, SFB618, 618/3-09, SFB/TRR43 A7]; BMBF(NGFN-Plus) [01GS08169-73, 01GS08150, 01GS08108]; HDSA Coalition for the Cure; EU (EuroSpin) [Health-F2-2009-241498, HEALTH-F2-2009-242167]; Helmholtz Association (MSBN, HelMA) [HA-215]; FCT [IF/00881/2013]info:eu-repo/semantics/publishedVersio