166 research outputs found
Repulsive bimodal atomic force microscopy on polymers
Bimodal atomic force microscopy can provide high-resolution images of polymers. In the bimodal operation mode, two eigenmodes of the cantilever are driven simultaneously. When examining polymers, an effective mechanical contact is often required between the tip and the sample to obtain compositional contrast, so particular emphasis was placed on the repulsive regime of dynamic force microscopy. We thus investigated bimodal imaging on a polystyrene-block-polybutadiene diblock copolymer surface and on polystyrene. The attractive operation regime was only stable when the amplitude of the second eigenmode was kept small compared to the amplitude of the fundamental mode. To clarify the influence of the higher eigenmode oscillation on the image quality, the amplitude ratio of both modes was systematically varied. Fourier analysis of the time series recorded during imaging showed frequency mixing. However, these spurious signals were at least two orders of magnitude smaller than the first two fundamental eigenmodes. Thus, repulsive bimodal imaging of polymer surfaces yields a good signal quality for amplitude ratios smaller than A 01/A 02 = 10:1 without affecting the topography feedback. © 2012 Gigler et al; licensee Beilstein-Institut.This work was financially supported by the European Commission (FORCETOOL, NMP4-CT-2004-013684) and the Excellence Cluster “Nanosystems Initiative Munich> (NIM).Peer Reviewe
Single Higgs-boson production through gamma-gamma scattering within the general 2HDM
The production of a single neutral Higgs boson h through (loop-induced)
gamma-gamma collisions is explored in the context of the linear colliders
within the general Two-Higgs-Doublet Model (2HDM). Two different mechanisms are
analyzed: on the one hand, the scattering gamma gamma-> h of two real photons
in a gamma-gamma collider; on the other, the more traditional mechanism of
virtual photon fusion, e+e- -->e+e- + h. Owing to the peculiar properties of
the Higgs boson self-interactions within the general 2HDM, we find that the
overall production rates can be boosted up significantly, provided the charged
Higgs mass is not too heavy. For example, if the latter is slightly above 100
GeV and, in addition, the lightest CP-even Higgs boson falls in the ballpark of
the LEP bound on the SM Higgs mass up to a few hundred GeV, the cross-sections
may typically render \sigma(gamma gamma-> h)= 0.1-1 pb and \sigma(e+e- --> e+e-
+ h)\sim 0.01 pb -- in both cases well above the SM prediction. Although for
charged Higgs masses above 300 GeV the rates become virtually insensitive to
the Higgs boson self-couplings, a significant tail of non-SM effects produced
by the combined contribution of the Yukawa couplings and gauge bosons could
still reveal a smoking gun.Comment: References and comments added. Accepted in Phys. Lett.
A non-canonical RNA silencing pathway promotes mRNA degradation in basal fungi
The increasing knowledge on the functional relevance of endogenous small RNAs (esRNAs) as riboregulators has stimulated the identification and characterization of these molecules in numerous eukaryotes. In the basal fungus Mucor circinelloides, an emerging opportunistic human pathogen, esRNAs that regulate the expression of many protein coding genes have been described. These esRNAs share common machinery for their biogenesis consisting of an RNase III endonuclease Dicer, a single Argonaute protein and two RNA-dependent RNA polymerases. We show in this study that, besides participating in this canonical dicer-dependent RNA interference (RNAi) pathway, the rdrp genes are involved in a novel dicer-independent degradation process of endogenous mRNAs. The analysis of esRNAs accumulated in wild type and silencing mutants demonstrates that this new rdrp-dependent dicer-independent regulatory pathway, which does not produce sRNA molecules of discrete sizes, controls the expression of target genes promoting the specific degradation of mRNAs by a previously unknown RNase. This pathway mainly regulates conserved genes involved in metabolism and cellular processes and signaling, such as those required for heme biosynthesis, and controls responses to specific environmental signals. Searching the Mucor genome for candidate RNases to participate in this pathway, and functional analysis of the corresponding knockout mutants, identified a new protein, R3B2. This RNase III-like protein presents unique domain architecture, it is specifically found in basal fungi and, besides its relevant role in the rdrp-dependent dicer-independent pathway, it is also involved in the canonical dicer-dependent RNAi pathway, highlighting its crucial role in the biogenesis and function of regulatory esRNAs. The involvement of RdRPs in RNA degradation could represent the first evolutionary step towards the development of an RNAi mechanism and constitutes a genetic link between mRNA degradation and post-transcriptional gene silencing
Communication, development, and social change in Spain: A field between institutionalization and implosion
This paper renders an account of the rapid institutionalization of the academic field of Communication for Development and Social Change (CDCS) in Spain in recent years following a period of neglect and marginalization. The ongoing expansion of the field of CDSC in the Spanish context is understood as a process of implosion, i.e. a collapse inwards, which results from the inconsistencies and weaknesses of fast and late institutionalization. The methodological approach for this inquiry is a documental review of both academic literature and research and institutional reports produced in Spain between 1980 and 2010. Based on this review, the paper contrasts the trajectory of the field in Spain with the debates at the international level, establishing relevant continuities and differences.This article is part of the Research Project (Ministry of Economy and Competitiveness,
Spain) CSO2014-52005-R titled ‘Evaluation and Monitoring of Communication for
Development and Social Change in Spain: design of indicators to measure its social
impact’ (2015–2017)17 página
Measurement of the cosmic ray spectrum above eV using inclined events detected with the Pierre Auger Observatory
A measurement of the cosmic-ray spectrum for energies exceeding
eV is presented, which is based on the analysis of showers
with zenith angles greater than detected with the Pierre Auger
Observatory between 1 January 2004 and 31 December 2013. The measured spectrum
confirms a flux suppression at the highest energies. Above
eV, the "ankle", the flux can be described by a power law with
index followed by
a smooth suppression region. For the energy () at which the
spectral flux has fallen to one-half of its extrapolated value in the absence
of suppression, we find
eV.Comment: Replaced with published version. Added journal reference and DO
Energy Estimation of Cosmic Rays with the Engineering Radio Array of the Pierre Auger Observatory
The Auger Engineering Radio Array (AERA) is part of the Pierre Auger
Observatory and is used to detect the radio emission of cosmic-ray air showers.
These observations are compared to the data of the surface detector stations of
the Observatory, which provide well-calibrated information on the cosmic-ray
energies and arrival directions. The response of the radio stations in the 30
to 80 MHz regime has been thoroughly calibrated to enable the reconstruction of
the incoming electric field. For the latter, the energy deposit per area is
determined from the radio pulses at each observer position and is interpolated
using a two-dimensional function that takes into account signal asymmetries due
to interference between the geomagnetic and charge-excess emission components.
The spatial integral over the signal distribution gives a direct measurement of
the energy transferred from the primary cosmic ray into radio emission in the
AERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air
shower arriving perpendicularly to the geomagnetic field. This radiation energy
-- corrected for geometrical effects -- is used as a cosmic-ray energy
estimator. Performing an absolute energy calibration against the
surface-detector information, we observe that this radio-energy estimator
scales quadratically with the cosmic-ray energy as expected for coherent
emission. We find an energy resolution of the radio reconstruction of 22% for
the data set and 17% for a high-quality subset containing only events with at
least five radio stations with signal.Comment: Replaced with published version. Added journal reference and DO
Measurement of the Radiation Energy in the Radio Signal of Extensive Air Showers as a Universal Estimator of Cosmic-Ray Energy
We measure the energy emitted by extensive air showers in the form of radio
emission in the frequency range from 30 to 80 MHz. Exploiting the accurate
energy scale of the Pierre Auger Observatory, we obtain a radiation energy of
15.8 \pm 0.7 (stat) \pm 6.7 (sys) MeV for cosmic rays with an energy of 1 EeV
arriving perpendicularly to a geomagnetic field of 0.24 G, scaling
quadratically with the cosmic-ray energy. A comparison with predictions from
state-of-the-art first-principle calculations shows agreement with our
measurement. The radiation energy provides direct access to the calorimetric
energy in the electromagnetic cascade of extensive air showers. Comparison with
our result thus allows the direct calibration of any cosmic-ray radio detector
against the well-established energy scale of the Pierre Auger Observatory.Comment: Replaced with published version. Added journal reference and DOI.
Supplemental material in the ancillary file
Deep Drilling in the Time Domain with DECam: Survey Characterization
This paper presents a new optical imaging survey of four deep drilling fields
(DDFs), two Galactic and two extragalactic, with the Dark Energy Camera (DECam)
on the 4 meter Blanco telescope at the Cerro Tololo Inter-American Observatory
(CTIO). During the first year of observations in 2021, 4000 images covering
21 square degrees (7 DECam pointings), with 40 epochs (nights) per field
and 5 to 6 images per night per filter in , , , and/or , have
become publicly available (the proprietary period for this program is waived).
We describe the real-time difference-image pipeline and how alerts are
distributed to brokers via the same distribution system as the Zwicky Transient
Facility (ZTF). In this paper, we focus on the two extragalactic deep fields
(COSMOS and ELAIS-S1), characterizing the detected sources and demonstrating
that the survey design is effective for probing the discovery space of faint
and fast variable and transient sources. We describe and make publicly
available 4413 calibrated light curves based on difference-image detection
photometry of transients and variables in the extragalactic fields. We also
present preliminary scientific analysis regarding Solar System small bodies,
stellar flares and variables, Galactic anomaly detection, fast-rising
transients and variables, supernovae, and active galactic nuclei.Comment: 22 pages, 17 figures, 2 tables. Accepted to MNRA
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